6,799 research outputs found
Multi-gene analysis for differentiation of aster yellows phytoplasmas infecting carrots in Serbia. Annals of Applied Biology, 154(2): 219-229.
No full textDuring a survey of large carrot fields in Serbia, plants showing leaf reddening
and/or yellowing, adventitious shoot production and reduction in taproot size
and quality were observed in a low percentage of plants. To verify phytoplasma
association with the described symptoms and to carry out pathogen differentiation,
PCR assays followed by restriction fragment length polymorphism (RFLP)
analyses and/or sequencing of phytoplasma 16Sr DNA and ribosomal protein
genes l22 and s3, tuf, putative aa kinase plus ribosomal recycling factor genes
and DNA helicase gene were carried out. Phytoplasmas belonging to 16SrI-A
and 16SrI-B ribosomal subgroups and to rpI-A and rpI-B ribosomal protein
subgroups, respectively, were identified by RFLP analyses in 13 of 15 symptomatic
plants tested. No amplification was obtained with non-symptomatic
carrot samples. The identification was confirmed by sequence analyses of the
phytoplasma genes studied. In two carrot samples, presence of interoperon
sequence heterogeneity was detected and phytoplasma strains were identified
as belonging to 16SrI group but were not assigned to any 16S rRNA or ribosomal
protein subgroup. This research allowed the first molecular identification
of phytoplasmas infecting carrot in Serbia using several molecular
markers, and it indicates that under field conditions in non-epidemic outbreaks
a certain amount of genetic mutation may occur in conserved genes of
these prokaryotes
Developing a method for phytoplasma identification in cactus pear samples from California.
No full textCactus pear plants showing proliferation and stunting of cladodes in Californian cultivations were tested in order to define a molecular
methodology for reliable phytoplasma detection. After several unsuccessful trials a simple extraction method was developed
to reduce the mucilage content in nucleic acid preparations that was seriously affecting pathogen detection. Nested PCR on
16S ribosomal gene and RFLP analyses together with sequencing of obtained amplicons allow to verify the presence in symptomatic
plants of 16SrV-A and 16SrI-B phytoplasmas respectively related to ‘Candidatus Phytoplasma ulmi’ and ‘Ca. P. asteris’
[Poesia] Três poemas de Alberto Secama
No full textThree poems by Alberto Secama. About the author: Alberto Secama is an Angolan poet who has poems published on many websites and on facebook:https://www.facebook.com/Xungurra/abouthttp://www.pordentrodaafrica.com/cultura/africa-em-verso-rio-kwanza-por-alberto-secamahttp://www.pordentrodaafrica.com/cultura/africa-em-verso-zong-por-alberto-secamahttp://www.pordentrodaafrica.com/cultura/coluna-africa-em-verso-o-sol-la-fora-por-alberto-secamaTres poemas de Alberto Secama. Sobre el autor: Alberto Secama es un poeta angoleño que tiene poemas publicados en varios sitios y en el facebook:https://www.facebook.com/Xungurra/abouthttp://www.pordentrodaafrica.com/cultura/africa-em-verso-rio-kwanza-por-alberto-secamahttp://www.pordentrodaafrica.com/cultura/africa-em-verso-zong-por-alberto-secamahttp://www.pordentrodaafrica.com/cultura/coluna-africa-em-verso-o-sol-la-fora-por-alberto-secamaTrês poemas de Alberto Secama. Sobre o autor: Alberto Secama é um poeta angolano que possui poemas publicados em vários sites e no facebook:https://www.facebook.com/Xungurra/abouthttp://www.pordentrodaafrica.com/cultura/africa-em-verso-rio-kwanza-por-alberto-secamahttp://www.pordentrodaafrica.com/cultura/africa-em-verso-zong-por-alberto-secamahttp://www.pordentrodaafrica.com/cultura/coluna-africa-em-verso-o-sol-la-fora-por-alberto-secam
Orizzonti mantovani. Spunti e dinamiche paesaggistiche ne L'Illustrissimo di Alberto Cantoni
No full textIn the literary production of Alberto Cantoni, short story writer and novelist between the nineteenth and twentieth centuries, the novel L'Illustrissimo is highly important both because it is the last publication of the author, from Pomponesco, a small town a few kilometers south of Mantua, both because it summarizes in a single text the different nuances and different directions that his writing has taken over the course of his literary career, also due to a writing and processing time that embraces the entire span of years of his career itself. In the foreground, in addition to the numerous and brilliant characters, one of the protagonists is the Mantuan landscape which, not a simple background, becomes a true literary parameter which in different and significant ways affects the purposes and mechanisms of the novel
Identification of 16SrIX group phytoplasmas infecting Colombian periwinkles and molecular characterization on several genes.
No full textPlenary Session: Luis Alberto Urrea
No full texta) Plenary Session: Luis Alberto Urrea, Mexican American Chicago Writer
Luis Alberto Urrea is a 2005 Pulitzer Prize finalist for non-fiction and member of the Latino Literature Hall of Fame. He is a prolific and acclaimed writer who has used his dual-culture life border experiences to explore the complex and interconnected Mexican-US American reality. The critically acclaimed and best-selling Mexican-born author of 13 books, Urrea has won numerous awards for his poetry, fiction and essays.
Moderator: Dr. Héctor García, Loyola University Chicag
O duplo significado do sentir e do pensar nas obras de Alberto Caeiro e Descartes
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em LiteraturaA pretensão desta dissertação intitulada O duplo significado do sentir e do pensar nas obras de Alberto Caeiro e Descartes é investigar a relação entre o sentir e o puro pensar, usando como referência a poesia O Guardador de Rebanhos, de Alberto Caeiro, que apresenta de forma evidente o tema; e como este assunto é tratado nas Meditações Metafísicas de René Descartes, também de forma claramente visível. Podemos perceber em ambos os autores, uma diplopia, uma espécie de duplo olhar sobre a relação sentir e puro pensar. No primeiro capítulo vou falar sobre a tese do duplo olhar de Descartes, a respeito da relação sentir e puro pensar na leitura de Merleau-Ponty, salientando a visão de natureza. No segundo capítulo, parto da leitura das três primeiras Meditações Metafísicas para caracterizar a tensão que se pode verificar entre Descartes e Alberto Caeiro quando se referem ao puro pensar. O foco principal de Descartes, nas três primeiras Meditações, é o pensar puro, e neste ponto Alberto Caeiro critica o pensar puro. No terceiro capítulo, analisando as três últimas Meditações, percebe-se que há uma virada no pensamento de Descartes, no qual o autor inverte seu ponto de vista, aproximando-se da idéia de Alberto Caeiro. No quarto capítulo, mostrarei a leitura que outros autores fazem acerca do sentir e do pensar na obra de Alberto Caeiro, mostrando que assim como Descartes, este também ostenta uma diplopia, não do sentir, mas do pensar. The pretension of this work entitled O duplo significado do sentir e do pensar nas obras de Alberto Caeiro e Descartes is to investigate the relationship between the feeling and the pure thinking, using as reference the poetry O Guardador de Rebanhos, by Alberto Caeiro, that presents in an evident way this theme; and as this subject is treated in René Descartes' Metaphysical Meditations, also in a clearly visible way. It is possible to notice in both authors, the diplopy, a sort of double look about the relationship between feeling and pure thinking. The thesis about Descartes'double look regarding the relation of feeling and pure thinking, in Merleau-Ponty reading, pointing out the nature vision, will be treated in the first chapter. In the second chapter, I start from the reading of the first three Metaphysical Meditations to characterize the tension that can be verified between Descartes and Alberto Caeiro when they refer to the pure thinking. Descartes' main focus in the first three Meditations is pure thinking and, in this point, Alberto Caeiro criticizes pure thinking. In the third chapter, analyzing the last three Meditations, it is possible to notice that there is an alteration in Descartes' thought, in which the author inverts his point of view, approaching of Alberto Caeiro's idea. In the fourth chapter, I will show the reading that other authors do concerning feeling and thinking in Alberto Caeiro's work, showing that as well as Descartes, this also shows a diplopy, not of feeling, but of thinking
Phytoplasma detection in corn with reddening in Italy
No full textDuring the second half of August 2009 in corn fields located in Northern Italy scattered plants showing reddening symptoms were observed, mainly located at the edge of the fields. Symptoms were clearly visible on the main leaf midribs, and/or on the stalks, and eventually affect the whole plant. Symptomatic plants had smaller size than healthy ones, and corn cobs were sometime malformed and of very little size. In some of the symptomatic plants the cobs produced were of regular size and contains poor shrivelled grains as reported for reddening disease of corn in Serbia (Duduk & Bertaccini, Plant Disease, 90, 1313-1319. 2006). Ten samples of symptomatic, and 4 of asymptomatic corn plants were collected in two different locations and nested PCR assays were carried out on total nucleic acids from 1 g of main leaf midrib and phloem stalk tissues chloroform/phenol extracted. Direct PCR assays with phytoplasma universal primer pair P1/P7 followed by nested PCR with 16S758F/16S1242R (Gibb et al., Phytopathology, 85, 169-174. 1995) primers allowed amplification of 500 bp amplicons from all samples from symptomatic plants, no bands were obtained from asymptomatic samples. Identification of detected phytoplasmas done using RFLP analyses with TruI, Tsp509I and MboII restriction enzymes allow preliminary identification of phytoplasmas belonging to 16SrI (aster yellows), 16SrIII (X disease) and 16SrXII (stolbur) groups, in same cases in mixed infection. Further molecular characterization of these phytoplasmas is in progress together with epidemiological studies to verify the presence of phytoplasma sources, and of possible insect vectors in the two environments. Presence of stolbur phytoplasmas in corn samples with reddening symptoms is confirming the finding in Serbia (Duduk & Bertaccini, above), however this is the first report in Europe of 16SrI group phytoplasmas, and the first report of 16SrIII in corn. The diverse phytoplasmas are associated with indistinguishable symptoms in plants as already worldwide reported in this and in other plant species for phytoplasma infection
Molecular identification of “Bois Noir” phytoplasmas in grapevine in Bulgaria.
No full textField inspection in 4 vineyards located near Plovdiv (Bulgaria) allows observation of yellows symptom presence. PCR/RFLP
analyses on 16S ribosomal gene identified phytoplasmas belonging to 16SrXII-A ribosomal subgroup in both symptomatic grapevines
and bindweed growing in infected vineyard. Grapevine mother plant and young plants were also infected suggesting that,
even if this is the first report of this disease in Bulgaria, the environment is epidemically affected
Lobbying for change as a new theory and practice of active citizenship: author interview with Alberto Alemanno
No full textDo you believe that lobbying is a vehicle for representing the interests of some rather than all, meaning its actors can never be a force for good? Alberto Alemanno, author of Lobbying for Change: Find Your Voice to Create a Better Society, might convince you of the contrary. This interview with Barbara Aubin, LSE Public Affairs, reflects on the importance of engaging with our political representatives, holding them accountable and lobbying for the causes we deeply care about as fundamental elements of a healthy democracy
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