984 research outputs found
Construction of E.coli-C.acetobutylicum shuttle vectors and transformation of C.acetobutylicum strains
No full textSeveral E. coli-C. acetobutylicum shuttle vectors were constructed and used to transform two C. acetobutylicum strains ATCC 824 and NCIMB 8052. Other than pSYL2, none of these vectors were able to transform ATCC 824 due to the presence of a restriction system. However, all of them could transform NCIMB 8052 with efficiencies of 8 x 10(2) - 6 x 10(3) transformants per mu-g DNA.This work was carried out at Northwestern University and was supported by Grant #BCS-8912209 from the National Science Foundation, USA
To Live Amongst the Dead: an Ethnographic Exploration of Mass Graves in Cambodia
Get PDFThis thesis uses mass graves as a lens through which to examine how people in contemporary Cambodia use the Khmer Rouge period (1975 – 1979) to reconstitute and re-imagine the world they live in. Based on sixteen months of multisited ethnographic fieldwork, this thesis will argue that the Khmer Rouge regime was a critical event (Das 1997) in Cambodian life, and as such has triggered a re-shaping of relationships between local and the national, and the national and the global, leading to new forms of social and community life and action in post Khmer Rouge Cambodia. As physical markers of violence and political instability, mass graves are inherently political and articulate these re-imaginations on the state, community, and individual level. The Cambodian state exercises and legitimates its authority by constructing modern history in reference to a narrative of liberation from the Khmer Rouge, and the ‘innocent suffering’ of Cambodia and its people, while local communities use Buddhism and animism to narrate and conceptualise the period, bringing it into expected and understandable events within Khmer Buddhist cosmology. These approaches are not necessarily in opposition to one another, but rather represent the overlapping plurality of connections with mass graves.
This thesis provides a unique exploration of social relationships to mass graves in Cambodia contributing to debates within the anthropology of politics, violence and collective memory by examining how moments of national mass violence re-shape the state and relationships within it, and how destructive periods of violence nonetheless create new fields for the imagination of the political, the religious, and the social. It also contributes to the emerging field of Cambodian ethnography that combines local considerations with wider national and geo-political discourses and how these are played out at the local level
GN-z11: The environment of an active galactic nucleus at
Get PDFRecent observations with the James Webb Space Telescope (JWST) have further refined the spectroscopic redshift of GN-z11, one of the most distant galaxies identified with the Hubble Space Telescope, at z = 10.603. The presence of extremely dense gas (> 1010 cm−3), the detection of high-ionisation lines and of CII*1335 emission, and the presence of an ionisation cone indicate that GN-z11 also hosts an active galactic nucleus. Further photometric and spectroscopic follow-up demonstrates that it lies in a large-scale, overdense structure with possible signatures of Population III stars in its halo. Surprisingly, Lyα has also been detected despite the expected largely neutral intergalactic medium at such a redshift. We exploit recent JWST/NIRSpec integral field unit observations to demonstrate that the Lyα emission in GN-z11 is part of an extended halo with a minimum size of 0.8–3.2 kpc, depending on the definition used to derive the halo size. The surface brightness of the Lyα halo around GN-z11 appears consistent with Lyα halos observed around z ∼ 6 quasars. At the wavelength of Lyα at z ∼ 10.6, we identify three other emission line candidates within the integral field unit field of view with no UV rest-frame counterpart visible in deep images from the JWST/NIRCam. If confirmed, this could be the first evidence that the local region of GN-z11 represents a candidate protocluster core, forming just 400 Myr after the Big Bang. We give a first estimate of the dark matter halo mass of this structure , which is consistent with a Coma-like cluster progenitor
On some properties of singular matrices
No full textAbstractSome results of Ostrowski in [5] are generalized to the case of monotonic norms
CadC-mediated activation of the cadBA promoter in Escherichia coli
Get PDFThe transcriptional activator CadC in Escherichia coli, a member of the ToxR-like proteins, activates transcription of the cadBA operon encoding the lysine decarboxylase CadA and the lysine-cadaverine antiporter CadB. cadBA is induced under conditions of acidic external pH and exogenous lysine; anoxic conditions raise the expression level up to 10 times. To characterize the binding mechanism of CadC, procedures for the purification of this membrane-integrated protein and its reconstitution into proteoliposomes were established. The binding sites of CadC upstream of the cadBA promoter region were determined by in vitro DNaseI protection analysis. Two regions were protected during DNaseI digestion, one from - 144 to - 112 bp, designated Cad1, and another one from - 89 to - 59 bp, designated Cad2. Binding of purified CadC to Cad1 and Cad2 was further characterized by DNA-binding assays, indicating that CadC was able to bind to both DNA fragments. Genetic analysis with promoter-lacZ fusions confirmed that both sites, Cad1 and Cad2, are essential for activation of cadBA transcription. Moreover, these experiments revealed that binding of H-NS upstream of the CadC-binding sites is necessary for repression of cadBA expression at neutral pH and under aerobic conditions. Based on these results, a model for transcriptional regulation of the cadBA operon is proposed, according to which H-NS is involved in the formation of a repression complex under non-inducing conditions. This complex is dissolved by binding of CadC to Cad1 under inducing conditions. Upon binding of CadC to Cad2 cadBA expression is activated. Copyright (C) 2005 S. Karger AG, Basel
Surveying the open podcast landscape and finding our niche: Blog #1 for the Open Podcasting Project
No full textblog post#GO--GN Fellowshiphttps://go-gn.net/gogn/the-third-call-for-go-gn/Open educational practices (OEP)Student perspectivesOpen researchpodcast creatio
Introducing the "Into the Open" podcast series
No full textblog post#GO--GN Fellowshiphttps://go-gn.net/gogn/the-third-call-for-go-gn/Open Educational Practices (OEP)Student perspectivesOpen researchPodcast creatio
Small points on subvarieties of a torus
Get PDFLet V be a subvariety of a torus defined over the algebraic numbers. We give a qualitative and quantitative description of the set of points of V of height bounded by invariants associated to any variety containing V . Especially, we determine whether such a set is or is not dense in V . We then prove that these sets can always be written as the intersection of V with a finite union of translates of tori of which we control the sum of the degrees. As a consequence, we prove a conjecture by the first author and David up to a logarithmic factor
Altura de la rama mandibular y su relación con el ángulo SN GO-GN en radiografías cefálicas laterales digitales
Get PDFObjetivo: El presente estudio de investigación tiene como objetivo establecer la altura de la rama mandibular y su relación con el ángulo SN Go-Gn en radiografías cefálicas laterales digitales en pacientes de 18 a 45 años de edad de la ciudad de Cuenca-Ecuador. Materiales y Métodos: Este estudio, cuantitativo, observacional con un enfoque correlacional, se realizó en radiografías cefálicas laterales digitalizadas que reposan en el archivo de un centro radiológico de la ciudad de Cuenca - Ecuador e incluyó un total de 388 radiografías laterales escogidas bajo ciertos criterios de inclusión y exclusión. Las variables analizadas fueron altura de la rama mandibular, ángulo SN Go-Gn y sexo. Las medidas se realizaron, aplicando el software AUTOCAD 2018, por un examinador entrenado previamente para tal fin, y validados posteriormente por la autora del estudio. Los datos obtenidos de las diferentes mediciones fueron registrados en una hoja de cálculo Excel. Para evaluar la relación entre la altura de la rama mandibular y el ángulo SN Go-Gn se empleó el coeficiente de correlación r de Pearson, para la interpretación se siguió la sugerencia de Cohen (1988); correlación nula (0.00 ≤ r <0.10), correlación leve (0.10 ≤ r <0.30), correlación moderada (0.30 ≤ r <0.50) y correlación fuerte (0.50 ≤ r < 1). Para la comparación se empleó el estadístico paramétrico t-Student, la significancia considerada fue de 0.01 (p<0.01). El procesamiento de datos fue realizado en el programa estadístico SPSS V27. Resultados: Se encontró una correlación negativa fuerte, entre la altura de la rama mandibular y el ángulo SN Go-Gn (r= -0.535; p<0.001), lo que implica que a mayor altura (mm), menor ángulo (°) Conclusión: Los resultados mostraron que si existe relación entre la altura de la rama mandibular y el ángulo SN Go-Gn, en pacientes de 18 a 45 años de edad.The present research study aims to establish the height of the mandibular ramus and its relationship with the SN Go-Gn angle in digital lateral cephalic radiographs in patients from 18 to 45 years old from the city of Cuenca-Ecuador. Materials and Methods: This study, quantitative, observational with a correlational approach, was carried out on digitalized lateral cephalic radiographs that rest in the archive of a radiological center in the city of Cuenca - Ecuador and included a total of 388 lateral radiographs chosen under certain criteria of inclusion and exclusion. The variables analyzed were mandibular ramus height, SN Go-Gn angle, and sex. The measurements were made, applying the AUTOCAD 2018 software, by an examiner previously trained for this purpose, and subsequently validated by the author of the study. The data obtained from the different measurements were recorded in an Excel spreadsheet. To evaluate the relationship between the height of the mandibular ramus and the SN Go-Gn angle, the Pearson r correlation coefficient was used, for the interpretation the suggestion of Cohen (1988) was followed; null correlation (0.00 ≤ r <0.10), slight correlation (0.10 ≤ r <0.30), moderate correlation (0.30 ≤ r <0.50) and strong correlation (0.50 ≤ r < 1). For the comparison, the parametric t-Student statistic was used, the significance considered was 0.01 (p<0.01). The data processing was carried out in the statistical program SPSS V27. Results: A strong negative correlation was found between the height of the mandibular ramus and the SN Go-Gn angle (r= -0.535; p<0.001), which implies that the higher the height (mm), the lower the angle (°). Conclusion: The results showed that there is a relationship between the height of the mandibular ramus and the SN Go-Gn angle, in patients from 18 to 45 years old.
Key words: mandibular Ramus, mandibular growth0000-0002-3794-1942Especialista en OrtodonciaCuenc
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