162,421 research outputs found
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Structural determinants of salmon calcitonin bioactivity -the role of the leu-based amphipatic alfa-helix
No full textSalmon calcitonin (sCT) forms an amphipathic helix in the region 9-19, with the C-terminal decapeptide interacting with the helix (Amodeo, P., Motta, A., Strazzullo, G., Castiglione Morelli, M. A. (1999) J. Biomol. NMR 13, 161-174). To uncover the structural requirements for the hormone bioactivity, we investigated several sCT analogs. They were designed so as to alter the length of the central helix by removal and/or replacement of flanking residues and by selectively mutating or deleting residues inside the helix. The helix content was assessed by circular dichroism and NMR spectroscopies; the receptor binding affinity in human breast cancer cell line T 47D and the in vivo hypocalcemic activity were also evaluated. In particular, by NMR spectroscopy and molecular dynamics calculations we studied Leu(23),Ala(24)-sCT in which Pro(23) and Arg(24) were replaced by helix inducing residues. Compared with sCT, it assumes a longer amphipathic alpha-helix, with decreased binding affinity and one-fifth of the hypocalcemic activity, therefore supporting the idea of a relationship between a definite helix length and bioactivity. From the analysis of other sCT mutants, we inferred that the correct helix length is located in the 9-19 region and requires long range interactions and the presence of specific regions of residues within the sequence for high binding affinity and hypocalcemic activity. Taken together, the structural and biological data identify well defined structural parameters of the helix for sCT bioactivity
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
Mr. Melvin J. Collier, RWWL AUC, June 2011
No full textThis video is a conversation with Mr. Melvin J. Collier. Mr. Collier talks about his book, "From Mississippi to Africa: A Journey of Discovery". Daniel Le, AUC Woodruff Library, is the interviewer
Analysis of the SARS-CoV-2 inactivation mechanism using violet-blue light (405 nm)
No full textThe COVID-19 pandemic has highlighted the urgent need for efficient and sustainable disinfection solutions that can reduce the spread of airborne and surface infections. Light-based disinfection technologies have attracted significant interest due to their non-invasive properties, adaptability and environmental benefits. Violet-blue light (VBL) at 405 nm has been shown to be an effective method, inducing the production of reactive oxygen species (ROS) that reduce microbial viability. This work aims to clarify the inactivation processes of SARS-CoV-2 during VBL exposure, thereby aiding the development of novel antiviral methods.
The study assessed the effects of VBL on cell viability, replication, carbonylation of 3 structural proteins (spike (S), envelope (E) and nucleoprotein (N)) and 1 non-structural protein (NSP13 helicase), and direct damage to viral RNA. SARS-CoV-2 was exposed to increasing doses of VBL along with influenza A and B viruses to assess susceptibility to VBL compared to other airborne RNA viruses. At the higher dose, SARS-CoV-2 was significantly more susceptible to VBL compared to influenza viruses, with 2.33 log10 viral titer reduction after 90’ exposure at 21.6 J/cm2.
Viral RNA after exposure to VBL showed no significant changes as assessed by NGS and qRT-PCR, suggesting that the inactivation process does not involve direct nucleic acid damage. Cell viability experiments were performed using different dilutions of DMEM medium in PBS (1:3, 1:20 and 1:1,000) to exclude the responsibility of the culture suspension in the inactivation process. The results indicated that the suspension medium played a minor role in virus inactivation, as viability did not increase with increasing dilution of DMEM. Subsequent tests with three different antioxidants (NAC, AsA and SOD) at different concentrations contributed to counteract the oxidative process and increase viability up to a maximum of 14.33% (with SOD 0.003 mM). Carbonylation of S and E protein was more pronounced when viruses were suspended in DMEM medium containing photosensitive molecules, although tests showed that the intrinsic properties of the viral membrane were a crucial element to consider in relation to its susceptibility to VBL. Indeed, lipid peroxidation has been identified as an important factor affecting the structural integrity and function of the viral envelope, impairing the ability of the virus to interact with host cells and leading to non-infectivity. The SARS-CoV-2 envelope lipidomic, composed predominantly of glycerophospholipids and devoid of cholesterol and sphingolipids, appears to be the critical factor in its susceptibility, distinguishing it from influenza viruses, which have a lipid profile richer in protective components against oxidative stress.
The results highlight the efficacy of VBL as a non-invasive disinfection method for use in healthcare, public areas and air filtration systems. In contrast to conventional UV disinfection techniques, VBL operates at safer wavelengths, reducing the risks associated with exposure. This makes it a particularly attractive choice for environments that require regular and safe disinfection.
In conclusion, VBL offers a realistic option for viral inactivation, particularly against SARS-CoV-2. Further research should improve VBL procedures, investigate its synergistic interactions with other disinfection techniques, and evaluate its efficacy in practical applications to facilitate its use as a novel and efficient disinfection approach
A Tripartite Post-Recession Rebalancing
No full textIn this latest Advance & Rutgers Report, entitled “A Tripartite Post-Recession Rebalancing,” Dean James W. Hughes and Professor Joseph J. Seneca deliver an incisive assessment of the current market conditions and obstacles in the path of our economic recovery. They offer a statistical cautionary tale that the private and public sector need to hear and acknowledge in order for the economy to make continued progress.This report was published as Issue Paper Number 7, November 2011, in Advance & Rutgers Report
Evidence for the decay B0→J/ψω and measurement of the relative branching fractions of meson decays to J/ψη and J/ψη′
No full textFirst evidence of the B 0 → J / ψ ω decay is found and the B s 0 → J / ψ η and B s 0 → J / ψ η ′ decays are studied using a dataset corresponding to an integrated luminosity of 1.0 fb -1 collected by the LHCb experiment in proton-proton collisions at a centre-of-mass energy of sqrt(s) = 7 TeV. The branching fractions of these decays are measured relative to that of the B 0 → J / ψ ρ 0 decay:frac(B (B 0 → J / ψ ω), B (B 0 → J / ψ ρ 0)) = 0.89 ± 0.19 (stat) - 0.13 + 0.07 (syst),frac(B (B s 0 → J / ψ η), B (B 0 → J / ψ ρ 0)) = 14.0 ± 1.2 (stat) - 1.5 + 1.1 (syst) - 1.0 + 1.1 (frac(f d, f s)),frac(B (B s 0 → J / ψ η ′), B (B 0 → J / ψ ρ 0)) = 12.7 ± 1.1 (stat) - 1.3 + 0.5 (syst) - 0.9 + 1.0 (frac(f d, f s)), where the last uncertainty is due to the knowledge of f d / f s, the ratio of b-quark hadronization factors that accounts for the different production rate of B 0 and B s 0 mesons. The ratio of the branching fractions of B s 0 → J / ψ η ′ and B s 0 → J / ψ η decays is measured to befrac(B (B s 0 → J / ψ η ′), B (B s 0 → J / ψ η)) = 0.90 ± 0.09 (stat) - 0.02 + 0.06 (syst)
The vanishing author in computer-generated works: a critical analysis of recent Australian case law
No full textAbstract
The use of software is ubiquitous in the creation of many copyright works, yet the requirement in copyright law that every work have a human author who engages in independent intellectual effort means that its use may prevent copyright subsistence. Several recent Australian cases have refocused attention on authorship as an essential criterion of copyright subsistence, and these cases suggest that much computer-produced output may be authorless and thus lack copyright protection. This article, the first in a two-part series, analyses how each case deals with the question of authorship of computer-produced works and why the use of software diminishes copyright protection for a significant number of computer-generated works. The article critiques the application of conventional notions of human authorship developed in the pre-computer age to modern productions and suggests alternative approaches to authorship that satisfy both the major objectives of copyright policy and the need to adapt to the computer age. The article argues that, without a broader judicial approach to authorship of computer-generated works, Parliament must remedy the lacuna in protection for these ‘authorless’ works. Possible solutions for reform are suggested. In a forthcoming article, the author comprehensively examines those reform proposals
Identity complexity and integration in lesbian, gay, bisexual and heterosexual adolescents and emerging adults: Implications for clinical practice
No full textThe present study aimed at assessing whether differences exist in identity complexity and integration between 31 lesbian, gay, and bisexual (LGB) and 33 heterosexual youths (mean age 21.47, SD = 3.27), both Italian and US. Participants completed a newly created questionnaire, the Identity Labels and Life Contexts Questionnaire (ILLCQ), which assesses the interplay between identity dimensions and life contexts. The ILLCQ assesses identity integration on three levels: (a) integration among the different domains of identity in their intersection with the various life contexts (assessed through salience and centrality); (b) integration between an individual’s self-definition and the definition of self made by others (perceived self-recognition); and (c) the integration between how the person perceives her/himself to be and the way she/he shows her/himself to others. Results suggest that identity salience varies significantly across life contexts for both LGB and heterosexual youths. The only significant difference between the LGB and heterosexual groups was higher salience and centrality of the sexual orientation domain for LGB youths. Sexuality represents a core identity domain for LGB participants, and perhaps less so for heterosexual participants. LGB youths reported lower general identity recognition from other people. Implications for clinical practice are discussed
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