342 research outputs found
Alt(r)o gradimento. Guerrini, la saga di Buttiglione e altri innesti
No full textRappresentante esemplare di una generazione di giovani intellettuali e artisti eclettici, attivi in diversi ambiti dell’industria culturale, Giacomo (Mino) Guerrini uno dei segreti meglio custoditi di sempre della storia del cinema italiano. Guerrini è stato molte cose: oltre che uomo di cinema – i cui film spaziano dalla commedia all’italiana all’horror, dalla spy-story al comico popolare – anche pittore e artista, giornalista e scrittore, autore televisivo e radiofonico. Un uomo di spettacolo e un intellettuale capace di muoversi su più piani e in diversi ambiti, senza mai preoccuparsi di distinguere tra cultura alta e bassa. L'intervento si focalizza in particolare sulla tetralogia di pellicole dedicate al popolare personaggio del Colonnello Buttiglione, ideato da Mario Marenco per la trasmissione radiofonica "Alto gradimento": un primo, sgangherato esempio di intermedialità da valutare tuttavia con un'attenzione che ne metta in luce, insieme agli innumerevoli difetti, gli elementi d'interesse.An exemplary representative of a generation of young, eclectic intellectuals and artists, active in various areas of the cultural industry, Giacomo (Mino) Guerrini was one of the best preserved secrets ever in the history of Italian cinema. Guerrini was many things: besides being a man of cinema - whose films range from Italian comedy to horror, from spy-story to popular comedy - he was also a painter and artist, journalist and writer, television and radio author. A man of show business and an intellectual capable of moving on many levels and in different areas, without ever bothering to distinguish between high and low culture. The essay focuses in particular on the film tetralogy dedicated to the popular character of Colonel Buttiglione, created by Mario Marenco for the radio program "Alto gradimento": a first, ramshackle example of intermediality to be evaluated, however, with an attention that highlights, along with the many flaws, the elements of interest
Rural Communities
No full textRecords describing life and organization in rural communities, particularly how the national government maintains relations with these villages.Royal Lao Government. "Rural Communities, Cantons or Tassengs, Villages or Bans" Excerpted from "Administrative Organization of Laos" by Dominique Guerrini, UN Expert in Public Administration, circa 196
SUBTELOMERIC AS WELL AS TELOMERIC SEQUENCES ARE LOST FROM CHROMOSOMES IN PROLIFERATING B-LYMPHOCYTES
No full textB lymphocytes purified from peripheral blood can be normally cultured in vitro for only one doubling. They can undergo an unlimited number of cell divisions after transformation with a DNA tumor virus such as the Epstein-Barr virus. We have shown that the terminal restriction fragments of virus transformed B lymphocytes are shortened in the course of proliferation and that this process is accompanied by structural modifications. We have identified the sequences that are lost during the shortening process by hybridization to the canonical human telomeric simple repeat TTAGGG, to other simple sequences that are found at the ends of human chromosomes, and to a human subtelomeric sequence. We have observed that by 20 doublings over half the TTAGGG sequences, but few or no TGAGGG sequences, are lost from the TRFs. The subtelomeric sequence was removed from most of the TRFs on which it was present. The implications that these observations have on the problems of cell senescence and oncology are discussed
Yeast linear plasmid with T(2)AG(3) telomeres: TEL+CEN antagonisms and genetic and molecula stability
No full textA linear plasmid containing ARS1, CEN4, and 48 bp of vertebrate (T(2)AG(3)) telomeric sequences at each end was used to transform Saccharomyces cerevisiae. Only circular plasmids that had lost the centromere and had retained the T(2)AG(3) sequences were obtained, indicating that the vertebrate T(2)AG(3) sequences and the yeast CEN4 could not be simultaneously present in this vector. This hypothesis was verified by removing the CEN4 sequence from the construct. In fact, the resulting transformants contained two classes of efficiently replicating linear plasmids: one of the expected size and one about twice as large. During subsequent growth, plasmids of the former, but not latter, class were subjected to concatemer formation. This can best be explained by recombination events involving the T(2)AG(3) sequences al the ends of the molecule, since very similar centric and acentric linear plasmids bearing Tetrahymena telomeric ends replicated faithfully
ON THE LEVEL OF PLASMID-BEARING CELLS IN TRANSFORMED CULTURES OF SACCHAROMYCES-CEREVISIAE
No full textLCl, a YIP5-derived plasmid containing a human DNA fragment with ARS activity in yeast, has been used to study the replication of ARS plasmids in Saccharomyces cerevisiae. ARS plasmids carried in yeast hosts are normally mitotically unstable. In transformed cultures the fraction of cells that contain plasmid, measured by plating on selective media, is lower than would be expected from measured rates of plasmid loss. In the case of S. cerevisiae carrying either the plasmid LCl or YRP17, the assay yields values of the order of 10-20% or 30-50% respectively. We have found that by doing a double nutritional upshift that involves conditioned medium and casamino acids, a population of cells can be defined that carry plasmid but are unable to grow on media that select for the plasmid marker. Thus the total fraction of cells that can be shown to contain plasmid increases to greater than 70%. To distinguish between the inability of plasmid to replicate in these cells and lack of expression of the selectable gene, cultures grown from single cells were analysed for the presence of plasmid DNA. In a substantial fraction of the population, plasmid DNA could be detected only by polymerase chain reaction and not by standard blotting and hybridization. These results suggest that plasmid is unable to replicate in these cells. Growth kinetics experiments with transformed cultures are consistent with the notion that only a small fraction of the cells contains plasmid capable of replication upon dilution into selective medium. Possible explanations for the phenomena observed are discussed
Determination of plasmid copy number in yeast transformants by means of agarose plugs
No full textThe determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection. The determination is based essentially on quantitative Southern hybridization of an appropirate probe to a sequence present both on plasmid and chromosomal DNA in digested or undigested samples run out on conventional agarose gels. The DNA extraction procedure calls for treatment of cell lysates with organic solvents that could entail systemic losses of eithr plasmid or chromosomal DNA thus producing artifactual results. We propose here a method based on the assumption that quantitative analysis of plasmid and chromosomal DNA extracted from yeast cells embedded in agarose plugs will furnish more reliable results. With this procedure the cells are lysed in situ, thus avoiding possible losses of material, and the chromosomes and plasmid DNAs, trapped within the agarose matrix, can be separated by pulse field electrophoresis
Il collezionista e il bibliotecario: Ugo Da Como, Paolo Guerrini e la storia della stampa a Brescia
No full textThe paper reconstructs the relationship between Senator Ugo Da Como (1869-1941), a collector, and the priest Paolo Guerrini (1880-1960), a local historian. The sources are mainly documents preserved in the Foundation Ugo Da Como (Lonato del Garda), which offer interesting informations on the making of the personal book collection of Da Como and, more generally, on the history of printing in Brescia. The author further emphasizes a less known aspect of the life of Guerrini, who was a prominent figure in the local antiquarian book trade during the Twenties and the Thirties of the 20th century
Empathy between Art and Design
No full textThis short essay delineates the art contest in which the notion of empathy developed between the late 19th and the early 20th century. Explores its meaning as the condition that makes intersubjectivity possible in Husserlian Phenomenology, especially in Edith Stein’s interpretation. It provides clues to understand the reasons that brought design culture focusing on empathy from the late 1990s onward. The increase of the participatory approach in design processes puts intersubjectivity – and therefore empathy – at the centre of any design practice.
Since Alice Devecchi, the author of the book in which the essay appears, focuses on empathy or, more precisely, on designing the empathic experience, the text provides comments and clues about the contest, the approach and the outcomes of her work – primarily focusing on the relevance of the arts as a means to increase design knowledge, an approach to research that Devecchi shares with the author, who also was the supervisor of her PhD thesis
Performance measurement system change:a consulting company perspective
No full textThis article explores how consulting companies develop
performance measurement system change (PMSC) through an analysis of the
processes, tools and techniques they adopt and the internal actors involved.
Moreover, it aims to demonstrate the existence of a situational approach in
changing a PMS related to the nature of services provided by each consulting
company. In a multiple case study, the author selected 18 consulting companies
that offer PMSC services and collected data through 36 partially structured
interviews (two managers from each company) and a brainstorming session
with three consultants from different firms. The data were analysed using
cross-case and within case analysis and descriptive statistics
Il catalogo di qualità : che vi sia ciascun lo dice, dove sia, nessun lo sa
No full textQuality is essential in any field of interest and activity (ref. UNI EN ISO 8402:1995 Quality management and quality assurance). What does quality mean in a library catalog? What are the requirements for quality? Quantitative measures are not enough to determine the quality of a catalog. The author proposes ten principles of qualitative analysis: authority control; cataloguing rules; competence and speedness of work; availability of databases and indexes; software and hardware used; clearness and readability of the catalog; reading ability; record functionality; every reader his catalog; maintenance frequency
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