1,759,337 research outputs found
Additional file 1 of Microglia-derived exosomes modulate myelin regeneration via miR-615-5p/MYRF axis
No full textAdditional file 1: Figure S1. The map of plasmid. A MYRF 3′UTR-Luciferase vector. B Mut MYRF 3′UTR-Luciferase vector. Figure S2. The expression of MBP and Iba1 in MS Lesion. A Immunofluorescence staining of MBP and Iba1 in brain of NAWM and MS lesion and B, C MBP fluorescence intensity and the number of Ibal+ cells per field. Scale bar = 100 μm. All data are represented by mean ± SEM (n = 5, each group). One-way ANOVA was used to determine P values (B, C). ****p < 0.0001. Figure S3. The expression of PDGFRα and MYRF. A After co-incubation of OPCs with supernatant, the expression of PDGFRα and MYRF and B the percentage of PDGFRα+MYRF+ cells were detected by immunofluorescence staining. C Immunofluorescence staining to determine the PDGFRα and MYRF expression intensity after OPCs treatment with EXOs/EXOs-LPS, and D the percentage of PDGFRα + MYRF + cells. One-way ANOVA was used to determine P values. *P < 0.05, **P < 0.01, ****P < 0.0001. Scale bar = 50 μm. Figure S4. Expression of miR-615-5p in EXOs/EXOs-LPS. A, B After exosomes were captured by CD81/CD9 chip, miR-615-5p was detected in EXOs/EXOs-LPS by Exoview. In addition, surface markers CD63 and CD9 of EXOs/EXOs-LPS were detected by Exoview. C, D ExoView detected the average colocalization percent of EXOs or EXOs-LPS. Figure S5. The co-expression of miR-615-5p and PDGFRα, APC, GFAP and NeuN. A Immunofluorescence staining and FISH detected the expression of PDGFRα and miR-615-5p in EAE/naïve spinal cords, and B the number of PDGFRα+miR-615-5p+ cells per field. C Immunofluorescence staining and FISH detected the expression of APC and miR-615-5p in EAE/naïve spinal cords, and D the number of APC +miR-615-5p+ cells per field. E Immunofluorescence staining and FISH detected the expression of GFAP and miR-615-5p in EAE/naïve spinal cords, and F the number of GFAP+miR-615-5p+ cells per field. G Immunofluorescence staining and FISH detected the expression of NeuN and miR-615-5p in EAE/naïve spinal cords, and H the number of NeuN +miR-615-5p+ cells per field. Scale bar = 50 μm. All data are represented by mean ± SEM (n = 5, each group). One-way ANOVA was used to determine P values. Figure S6. Expression of miR-615-5p. A The expression of miR-615-5p was detected by qRT-PCR in the brain and spinal cords of naïve/EAE mice. B The expression of miR-615-5p was detected by qRT-PCR in SM and SM-LPS. C The expression of miR-615-5p was detected by qRT-PCR in microglia, activated microglia, OPCs, and activated OPCs. D Raw264.7 was stimulated with 100 ng/ml LPS. FISH detected the expression of miR-615-5p in Raw264.7 and Raw264.7-LPS, and E the miR-615-5p fluorescence intensity. All data are mean ± SEM. t-tests were used to determine P values (B, E). One-way ANOVA was used to determine P values (C). Two-way ANOVA was used to determine p values (A). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One representative of three independent experiments is shown. Figure S7. Expression of miR-615-5p target genes. A–F After OPCs were incubated with SM-derived supernatant/SM-LPS-derived supernatant, qRT-PCR was used to detect the other target genes of miR-615-5p, including Plp2, Tcf7l2, Cdk5, Sox6. T-test was used to determine P values. **P < 0.01, ****P < 0.0001. One representative of three independent experiments is shown. Figure S8. Antagonistic miR-615-5p inhibited the expression of GFAP and APP. A Immunofluorescence staining of GFAP and APP in spinal cords of naïve and EAE mice, and B, C the number of GFAP+ cells and APP+ cells per field. Scale bar = 100 μm. All data are represented by mean ± SEM (n = 10, each group). One-way ANOVA was used to determine P values (B, C). ****p < 0.0001. One representative of three independent experiments is shown. Table S1. Primers for PCR. Table S2. Primers for qRT-PCR. Table S3. miR-615-5p target gene network expression table
The “Cybercrimes” phenomenon. A critical analysis of the crime of unauthorized access to a computer system or telematic system pursuant to Article 615 ter c.p.
No full textreservedCybercrimes
Articolo 615 ter c.p.Cybercrime
MOESM1 of The novel circular RNA circ-CAMK2A enhances lung adenocarcinoma metastasis by regulating the miR-615-5p/fibronectin 1 pathway
No full textAdditional file 1 :Figure S1. Northern blot analysis of circ-CAMK2A expression in three pairs of LUAD and normal tissues. 18S was used as control reference. Figure S2. qRT-PCR analysis of CAMK2A expression in LUAD cells with circ-CAMK2A overexpression or knockdown. Figure S3. RIP assay detecting the enrichment of circ-CAMK2A by Ago2 in HCC827 cells. **p < 0.01. Figure S4. The effect of miR-615-5p on circ-CAMK2A expression. qRT-PCR analysis of circ-CAMK2A expression in A549 cell lines transfected with control or miR-615-5p inhibitors. **p < 0.01. Figure S5. The protein quantification of FN1, MMP2 and MMP9 in LUAD cell lines with miR-615-5p knockdown or overexpression. **p < 0.01
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Additional file 10 of Circular RNA circ-MTHFD1L induces HR repair to promote gemcitabine resistance via the miR-615-3p/RPN6 axis in pancreatic ductal adenocarcinoma
No full textAdditional file 10: Table S5. Downstream target genes of miR-615-3p predicted by ENCORI, miRDB, mirDIP, miRTarBase and TargetScan databases and the intersection of five databases
22nd and 25th days [of the festival dedicated] to Ishtar of Hattarina - CTH 615
No full textNotes on the tablet concordances listed in CTH 615 related to the 22nd and 25th days of the festival dedicated to Ishtar, the goddess of love, war, fertility, and sexuality, particularly worshipped in northern Mesopotamia, at the Assyrian cities of Nineveh, Ashur and Arbela (Erbil). Emmanuel Laroche translated this text as "22e-25e jours : à Istar de Hattarina,” and classified in the category of texts of festivals and cults dedicated to AN.TAH.SUMsar (Catalogue des textes Hittites, No. 615).Paper (1 sheet
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
615-loppukoestuksen päivitys RELION-alustalle
No full textTämän opinnäytetyön tavoitteena oli muokata RELION-alusta, adapteri sekä ohjelmistot
toimimaan 615-tuotteettelle ABB Oy Distribution Solutions-yksikölle. Järjestelmälle olisi tarvetta, sillä se lisää tarkkuutta ja lisäisi testikapasiteettia 615 tuotteita
valmistavilla tehtailla.
Testijärjestelmässä on käytetty elektroniikkaa, tietotekniikkaa ja mekaniikkaa.
Opinnäytetyö on suurimmaksi osaksi tietotekniikkaa ja LabView G-kielellä suoritettua
testiohjelman luomista ja muokkausta. Testiohjelman ja mekaniikan muokkaus toimivan kokonaisuuden muodostamiseksi 615-releen lopputestaukselle RELION-alustalla
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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