Pustaka Kesehatan
Not a member yet
642 research outputs found
Sort by
Pengaruh Minyak Ikan Lemuru (Sardinella longiceps) terhadap Ekspresi Tumor Necrosis Factor (TNF-α) Kartilago yang Diinduksi Complete Freund's Adjuvant (Effect of Lemuru (Sardinella longiceps) Fish Oil on Tumor Necrosis Factor (TNF-α) Expression of Cartilage that Induced by Complete Freund's Adjuvant)
Get PDFOsteoarthritis is a chronic joint disorder with weakening and disintegration of the joint cartilage followed by the growth of new bone and cartilage. TNF-α is one of the inflammatory mediator that plays an important role in the progression of osteoarthritis. In the others hands, lemuru (Sardinella longiceps) fish oils that contain EPA and DHA has an anti-inflammatory effect. The purpose of this study was to investigate the effect of lemuru fish oil on decreasing the expression of TNF-α in cartilage that induced by Complete Freunds Adjuvant. This was a experimental design study with randomized post test only control group design. The sample was divided into 2 groups: the negative control group (K-) and the treatment group (P), each group was divided into three subgroups based on duration of lemuru fish oils treatment, 7th, 14th and 21st days. TNF-α expression data was obtained from immunohistochemical observations on tibiofemoral articular cartilage. The result showed that the expression of TNF-α in the treatment group is lower than the negative control group and One Way Anova test showed that there is significant difference between groups (p = 0.000). We can conclude that lemuru (Sardinella longiceps) fish oil decreased the expression of TNF-α. Keywords: Osteoarthritis, TNF-α, lemuru (Sardinella longiceps) fish oil, cartilag
Analisis Profil Protein Ekstrak Aquades dan Etanol Daun Mimba (Azadirachta Indica A. Juss) dengan Metode SDS-PAGE (Protein Profile Analysis of Aquadest and Ethanol Extract of Neem Leaves by Means of SDS-PAGE Method)
Get PDFBackground: Neem leaf is used to increase body imune response, antivirus, antifungi,antibacterial, dan antioxidant. These biology activities was suspected because of proteinin neem leaf. Protein in plants can be used as pharmacogenetic identification becausesome proteins in plants posse characteristic can defense to some pathogenmcroorganism. The way to know protein in the organism is using profile protein analyticthrough SDS-PAGE method. SDS-PAGE separates protein molecules by size andparticular shapping and also can determine protein molecular weight. Protein profilescan be obtained with protein extracts using solvents. Protein is a polar compound whichis bonded to a polar solvent therefore chosen ethanol and aquadest as a liquid asextraction solvent . Purpose: To know the protein profile of aquadest and ethanolextracts of neem leaf by using SDS-PAGE. Method: This study uses neem leaf as asample, aquadest and 70% ethanol as the extraction solvent then analyzed using SDSPAGE.Result: The results showed 9 protein bands on the aquadest extract of neemleaf and 8 of protein bands on the ethanol extract of neem leaf. Conclusion: From thestudy, there is a difference between the amount of protein bands aquadest extracts ofneem leaf with ethanol extract of neem leaf.Keywords: aquadest extract, ethanol extract, neem leaf, protein profile, SDS-PAG
Efektivitas Chlorhexidine sebagai Inhibitor MMP-2 dan MMP-9 dalam Proses Dermonekrosis Akibat Racun Kumbang Tomcat (Paederus sp.) (The Effectiveness of Chlorhexidine as MMP-2 and MMP-9 inhibitor in Dermonecrotic Process due to Rove Beetles Toxin (Paederus sp.))
Get PDFPaederus dermatitis is dermonecrotic condition caused by Rove beetles (Paederus sp.). Paederin toxin come from endosymbiotic between rove beetles and Pseudomonas aeruginosa and can produces MMP-2 and MMP-9. Chlorhexidine is expected to be alternative therapy and prevention of dermatitis paederus due to it's MMP inhibitor and bacteriostatic effect. The study aimed to investigated the effectivity of chlorhexidine as MMP-2 and MMP-9 inhibitor in dermonecrotic process due to rove beetles toxin. Research groups consisted of negative control (paederin toxin), treatment 1 (paederin toxin + chlorhexidine 0,012%), treatment 2 (paederin toxin + chlorhexidine 0,06%), treatment 3 (paederin toxin + chlorhexidine 0,3%), and normal control (aquadest). As much as 9,3 µg paederin toxin and chlorhexidine are injected to the well of 8% acrylamide gel containing gelatin. Measuring of band density using ImageJ showed a decreasing of MMP-2 and MMP-9 band density. Negative control group was considered to have 1 of relative optical density. The result showed that chlorhexidine 0,06% can inhibits 21,6% MMP-2 activities and at 0,3% can inhibits 16,4% MMP-9 activities. One way ANOVA test show different significantly between negative control and treatment group (p<0,05). In conclusion chlorhexidine has potency as MMP-2 and MMP-9 inhibitor in dermonecrotic process due to Rove Beetles toxin. Keywords: Rove beetles(Paederus sp.), dermonrcrotic, MMP-2, MMP-9, chlorhexidin
Survei Pengetahuan dan Pilihan Pengobatan Jerawat di Kalangan Mahasiswa Kesehatan Universitas Jember (A Survey on Knowledge and Treatment Options of Acne Vulgaris Among Health Science Students of Universitas Jember)
Get PDFAcne vulgaris is a common disorder that may impact physically and psychologically. It can be treated either using appropriate self-medication or medical assistance. This present study aimed to explore the knowledge of acne vulgaris and its treatment ofchoice among health science students of Universitas Jember. Data were collected using a 44 item questionnaire. A total of 193 respondents from Faculty of Pharmacy (34 students), Faculty of Public Health (59 students), Faculty of Dentistry (27 students), Faculty of Medicine (29 students), and School of Nursing (44 students) participated in the study after giving their consents. All respondents have had acne at some points of their life. The average level of knowledge was 17.3 ± 3.6 (max score 30) and there was astatistically significant difference of means (p=0.002) among the respondents, based on their faculties. Self-medication (n=49; 58%) was more prefered than medical assistance (n=35; 41.7%). This study showed the need to increase awareness among young peopleto practice appropriate self-medication.Keywords: acne vulgaris, knowledge, treatment option
Penetapan Kadar Fenol Total dan Pengujian Aktivitas Antioksidan Minyak Jahe Gajah (Zingiber officinale var. officinale) (Determination of Total Phenolic Content and Antioxidant Activity of Jahe Gajah (Zingiber officinale var. officinale) Oil)
Get PDFJahe gajah (Zingiber officinale var. officinale) is one of export commodity plant in Indonesia. Part of jahe gajah often to be used is the rhizome, that content lots of essentials oil. One of the essential oil content is a phenolic compound. Phenolic compound is a natural product function as antioxidant reducing oxidative damage in the body that cause inflammation. The aim of this study was to determine the total phenolic content and antioxidant activity of jahe gajah oil. The total phenol content was determined using Folin-Ciocalteu method and the antioxidant activity assay was done using DPPH method. The total phenol content for jahe gajah oil was 0.626 ± 0.022 mg GAE/g . The IC50 of jahe gajah oil was 5,766 ± 0,087 µl/ml. Antioxidant activity from jahe gajah oil was suggested to be contributed by the phenolic compound and result from synergistic action of all constituents.
Keywords: essential oil, aktivitas antioxidant, phenolic content
 
Aktivitas Minyak Jahe Merah (Zingiber Officinale var. rubrum) Terhadap Nyeri Inflamasi pada Mencit Balb-C dengan Induksi CFA (Completed Freund’s Adjuvant) (Activity of Red Ginger Oil (Zingiber officinale var. rubrum) on Inflammatory Pain in Mice Balb-C Wi
Get PDFPain is a multidimentional experiences. Chronic pain is caused by inflammation. Nowdays, the treatment of chronic pain is still a challenge. Plants that known to have analgetic activity is red ginger (Zingiber officinale var. rubrum). This research observed the effect of red ginger oil on inflammatory pain. Inflammatory condition was induced by intraplantar injection of CFA (Completed Freund's Adjuvant). Twenty mice were divided into 4 groups i.e sham, negative control, red ginger oil in two different doses (100 and 200 mg/kg BW). Red ginger oil was administrated per oral once a day for seven consecutive days, a week after CFA injection. Latency time toward thermal stimulus was measured on days 0, 1, 3, 5, 7, 8, 10, 12, and 14 after CFA injection. Paw thickness at the ipsilateral site was also measured on days 0, 1, 3, 5, 7, 8, 10, 12 and 14 after CFA injection. The result showed that the administration of red ginger oil significantly increased latency time toward thermal stimulus compared to negative control. It also significantly reduced paw thickness especially at dose of 200 mg/kg BW. These findings suggested that administration of red ginger oil could treat the inflammatory pain, caused by CFA injection.
Keywords: red ginger oil, inflammatory pain, CFA, hyperalgesia
 
Aktivitas Antioksidan dan Kemampuan Proteksi terhadap Kerusakan DNA dari Protein Isolat Biji Melinjo (Gnetum Gnemon L.) Terhidrolisis Menggunakan Alkalase Terimobilisasi (Antioxidant Activity and Protection Ability Against DNA Damage of Isolate Protein Me
Get PDFHydrolyzed protein isolates of melinjo seed using free alcalase has been known to have antioxidant potential. However, free alcalase can only be used once. Enzymes immobilization technique with entrapment method would overcome this problem. The immobilized enzyme characteristic was observed by SEM and hydrolysis degree value. The success of hydrolysis process was observed by SDS-PAGE. Antioxidant activity test was determined by the ability to inhibit hydroxyl radical and protection against DNA damage in 1% agarose gel. The result showed that protein isolates from melinjo seed had been successfully hydrolyzed by immobilized alcalase. Following hydrolysis showed that immobilized alcalase was effective to hydrolyze protein up to four times. Based on hydroxyl radical reduction activity test, antioxidant activity of hydrolyzed protein using immobilized alcalase had the same as that of glutathione as positive control antioxidant activity. In addition, hydrolyzed protein using immobilized alcalase had the ability toprotect DNA damage which showed by reversible change of supercoil band.
Keywords: melinjo seed, hydrolyzed protein, immobilized alcalase, antioxidan
Perlekatan Streptococcus mutans pada Aplikasi Fissure Sealant Berbahan Resin Dibandingkan dengan Ionomer Kaca Fuji VII (The Attachment of Streptococcus mutans for Fissure Sealant application Made of Resin Compare With Glass Ionomer Fuji VII)
Get PDFBackground : Dental caries is the hard tissue disease. Caries is caused by multiple factors that is of them are morphology the surface of the tooth and bacteria Streptococcus mutans. The pit and fissure are primary habitats of S. mutans. Therefore, prevention of fissure sealants are needed. The materials of fissure sealant are composite resin and glass ionomer fuji VII. S. mutans can attach to foreign objects, one of them is material restoration. Objective : The purpose of this research is to know the difference of S. mutans attachment for fissure sealant application made of resin and glass ionomer fuji VII. Method : The experimental laboratory was post test only control design. The samples size from two group are 32 samples. The tool that used is spectofotometer with unit of measurement is colony forming unit a mL. Result and Conclusion : The average of S. mutans attachtment of composite resin and glass ionomer are 5,201 x 108 and 3,963 x 108. The attachtment was tested by independent sample T-tes there was differences of S. mutans attachment for fissure sealant application made of resin and glass ionomer fuji VII. Resin composite have a higher total number than ionomer fuji VII.
Keywords: dental caries, fissure sealant, glass ionomer, resin, s. mutans,
 
Efektivitas Ekstrak Etanol Daun Bayam Merah sebagai Hepatoprotektor terhadap Kadar ALP Serum Mencit yang Diinduksi Isoniazid (Hepatoprotective Activity of Red Spinach (Amaranthus tricolor L.) Leaves Ethanolic Extract on ALP Serum Mice Induced by Isoniazi
Get PDFIsoniazid (INH) is metabolized become hidrazine that depleted glutation (GSH) in hepatocytes, resulted imbalance oxidant and antioxidant along with increased of free radicals, which can increase alkaline phosphatase (ALP). Ethanolic extract of red spinach (Amaranthus tricolor L.) leaves contains antioxidant which can donates the electron so free radical can be stabilized. The aim of this study was to investigate the effect of ethanolic extract of red spinach leaves on ALP levels of mice induced by INH and its effective dose. This research used 28 mices that divided into seven groups: K(N) was given normal saline, K(-) was given INH 100 mg/kgBW, K1, K2, K3, K4 and K5 group which given INH 100 mg/kgBW peroral and after 2 hours would be given ethanolic extract of red spinach leaves in the dose 1,05 mg/20gBW, 2,1 mg/20gBW, 4,2 mg/20gBW, 8,4 mg/20gBW and 16,8 mg/20gBW for ten days. ALP serum was measured on the day 11th.The result of average ALP levels were K(N) 41,36 U/L, K(-) 110,97 U/L, K1 79,26 U/L, K2 71,34 U/L, K3 66,51 U/L, K4 62,82 U/L and K5 47,21 U/L. In conclusion the ethanolic extract of red spinach leaves could prevent the increasing of ALP levels and the effective dose of the extract was 6,75 mg/20gBW.
Keywords: red spinach leaves, ALP, INH
 
Pengaruh Pemberian Niasin dan Kafein dalam Model Minuman Berenergi terhadap Fisiologi Hati Tikus Wistar Jantan (The Effect of Caffeine and Niacin Administration within Energy Drink Model towards Liver Physiology of Male Wistar Rat)
Get PDFRecently,there are many hepatotoxicity case reports induced by energy drinks and its niacin content is assumed to be the cause of liver injury. Caffeine in energy drinks actually has a hepatoprotective effect. However, this hepatoprotective effect of caffeine is less potent in counteracting the hepatotoxic effect of niacin. This study aimed at determining niacin and caffeine influences in inducing the disruption of liver physiology and the dose of niacin and caffeine in energy drink model which can induce the disruption of liver physiology by measuring AST, ALT, and GGT. As many as 28 male albino wistar rats were divided into seven groups;1 control group and 6 experimental groups, for 49 days. At day 50th, the rats were terminated to measure the AST, ALT, and GGT. The AST and ALT enzyme levels were not significantly correlated (p> 0,05). Otherwise, the GGT enzyme level was significantly correlated (p<0,05). It was showed that niacin and caffeine elevate GGT enzyme at doses 0,491 mg/ 200gr body weight/ day niacin and 1,2275 mg/ 200gr body weight/ day caffeine. These doses are equal to the consumption of three packs of energy drinks per day in human.
Keywords: niacin, caffeine, energy drinks, AST, ALT, GGT