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Potential biomarker of circulating hsa-miR-1273g-3p level for detection of recurrent epithelial ovarian cancer
No full textÇevik, Nazife (Arel Author)PurposeOvarian cancer (OC) is first gynaecologic cancer that causes women death and epithelial ovarian cancer (EOC) is the most lethal ovarian cancer type. While treatment is commonly successful, some cases (10-20%) show resistance to chemotherapy which is followed by recurrence. MicroRNA (miRNA) based diagnosis methods are slightly important for recurrent ovarian cancer diagnosis. We aimed to detect novel circulating miRNAs to be used as an early diagnosis and prediction tools for recurrent EOC.MethodsIn this study, recurrent EOC serum samples and healthy control serum samples were compared for miRNA expression analysis by microarray. Microarray results were analyzed by bioinformatics tools and differentially expressed hsa-miR-1273g-3p was obtained. After microarray analysis, differentially expressed hsa-miR-1273g-3p was validated by Real-Time PCR (RT-qPCR). The relation between target genes of hsa-miR-1273g-3p and ovarian cancer were examined by Pathway Studio((R)) (v.11.4.0.8).ResultsThe expression of hsa-miR-1273g-3p was found to be significantly down-regulated by t test Bonferroni FWER corrected p2, in recurrence EOC compare with healthy controls groups. The RT-qPCR results confirmed that relative expressions of the serum hsa-miR-1273g-3p were significantly down-regulated in patients with recurrent EOC (p=0.0275). Serum hsa-miR-1273g-3p levels could discriminate patients with recurrent EOC from healthy controls, with a power area under the curve (AUC) of 0.7.ConclusionThis study suggested that hsa-miR-1273g-3p plays a significant role in regulation of related genes, which are TNF-alfa, COL1A1, MMP-2, MMP-9, with recurrent EOC outcome. hsa-miR-1273g-3p may be used as a prognostic marker for recurrent EOC after chemotherapy
Computational insight into the phthalocyanine-DNA binding via docking and molecular dynamics simulations
No full textÖzbil, Mehmet (Arel Author)Phthalocyanines are considered as good DNA binders, which makes them promising anti-tumor drug leads. The purpose of this study is to investigate the interactions between DNA and quaternary metallophthalocyanine derivatives (Q-MPc) possessing varying metals (M = Zn, Ni, Cu, Fe, Mg and Ca) by molecular docking since there seems to be a lack of information in the literature regarding this issue. In this direction, Autodock Vina and Molegro Virtual Docker programs were employed. Autodock Vina results reveal that each Q-MPc derivative binds to DNA strongly with similar binding energies and almost identical binding modes. They bind to the grooves of DNA by constituting favorable interactions between phosphate groups of DNA and Q-MPcs. Although changing the metal has no significant effect on binding, presence of quaternary amine substituents increases the binding constant K-b by about 2-fold comparing to the core Pc (ZnPc). Contrary to Autodock Vina, the calculated Molegro Virtual Docker binding scores have been more diverse indicating that the scoring function of Molegro is better in differentiating these metals. Despite the fact that Molegro is superior to Autodock Vina in terms of metal characterization, Autodock Vina and Molegro exhibit similar binding sites for the studied metallophthalocyanines. We propose that Q-MPc derivatives designed in this study are promising anti-tumor lead compounds since they tightly bind to DNA with considerably high K-b values. Cationic substituents and presence of metal have both positive effects on DNA binding which is critical for designing DNA-active drugs. Additional calculations employing molecular dynamics (MD) simulations verified the stability of Q-MPc-DNA complexes which remained in contact after 20 ns via attractive interactions mainly between DNA backbone and the Pc metal center
Ufrs finansal araçlar standartları çerçevesinde riskten korunma muhasebesi ve bir uygulama
No full textYüksek lisans tezi.Muhasebe uygulamalarına yön veren kurallar bütünü olarak adlandırılan standartların temeli muhasebe ilkelerine, muhasebe ilkelerinin temeli ise muhasebenin temel kavramlarına dayanmaktadır. Muhasebe uygulamalarına yön veren bu standartlar ise aynı şekilde muhasebe politikalarını belirlemektedir. Tüm dünya ülkelerinin düzenlemeleri arasında bir standart olması amacıyla oluşturulan Uluslararası Muhasebe Standartları kurulu, çıkarılan Uluslararası Finansal Raporlama Standartları yayınlamaktadır. Türkiye Muhasebe Standartları bu standartların Türkçeye çevirisidir. Bu çalışmanın amacı riskten korunma muhasebesinde farklı dönemlerde rapor altına alınan kazanç ve kayıpları tutarsızlıkları yok ederek daha açık bir muhasebe kaydı oluşturmaktır. UFRS finansal araçlar standartları çerçevesinde riskten korunma muhasebesini ve işletmelerdeki kayıt tutarsızlığını gidermek amacıyla geliştirilmiş UFRS 9 Finansal Araçlar Standartları gereğince getirilen yeniliklerin ne olduğunu ve IAS 39 Finansal Araçlar: Muhasebeleştirme ve Ölçme standardından farklılığını ortaya koymaktır
New Mixed Conductivity Mechanisms in the Cold Plasma Device Based on Silver-Modified Zeolite Microporous Electronic Materials
No full textKoç, Emrah (Arel Author)We have analyzed the interaction between microdischarge and microporous zeolite electronic materials modified by silver (Ag-0) nanoparticles (resistivity similar to 10(11) to 10(6) Omega cm) on the atmospheric pressure cold plasma generation in air. The generation and maintenance of stable cold plasma is studied according to the effect of the Ag-0 nanoparticles. The role of charge carriers in mixed conductivity processes and electrical features of zeolite from low pressure to atmospheric pressure is analyzed in air microplasmas for both before and after breakdown regimes. The results obtained from the experiments indicate that Ag-0 nanoparticles play a significant role in considerably reducing the breakdown voltage in plasma electronic devices with microporous zeolite electronic materials
Assessment of ManSeg 2.6b application's accuracy [ManSeg 2.6b Uygulama Başarisinin De?erlendirilmesi]
No full text4th Electric Electronics, Computer Science, Biomedical Engineerings' Meeting, EBBT 2018 -- 18 April 2018 through 19 April 2018 --The present study aims to demonstrate a new fully/semi-automated segmentation tool, ManSeg 2.6b, for calculating the volume of the abdominal organs. In order to validate the accuracy, a phantom study was developed and the segmentation and volume computations were done with ManSeg 2.6b. The phantom study validated volume computation of the ManSeg 2.6b and 98.29%±0.88 and 97.33%±1.77 accuracies were obtained for 2mm and 5mm thicknesses respectively. © 2018 IEEE
Molecular genetic analysis of the F11 gene in 14 Turkish patients with factor XI deficiency: identification of novel and recurrent mutations and their inheritance within families
No full textÇakır, Volkan (Arel Author), Berber, Ergül (Arel Author)Background. Factor XI (FXI) deficiency is an autosomal bleeding disease associated with genetic defects in the F11 gene which cause decreased FXI levels or impaired FXI function. An increasing number of mutations has been reported in the FXI mutation database, most of which affect the serine protease domain of the protein. FXI is a heterogeneous disorder associated with a variable bleeding tendency and a variety of causative F11 gene mutations. The molecular basis of FXI deficiency in 14 patients from ten unrelated families in Turkey was analysed to establish genotype-phenotype correlations and inheritance of the mutations in the patients' families. Material and methods. Fourteen index cases with a diagnosis of FXI deficiency and family members of these patients were enrolled into the study. The patients' F11 genes were amplified by polymerase chain reaction and subjected to direct DNA sequencing analysis. The findings were analysed statistically using bivariate correlations, Pearson's correlation coefficient and the non-parametric Mann-Whitney test.Results. Direct DNA sequencing analysis of the F11 genes revealed that all of the 14 patients had a F11 gene mutation. Eight different mutations were identified in the apple 1, apple 2 or serine protease domains, except one which was a splice site mutation. Six of the mutations were recurrent. Two of the mutations were novel missense mutations, p.Val522Gly and p.Cys581Arg, within the catalytic domain. The p.Trp519Stop mutation was observed in two families whereas all the other mutations were specific to a single family. Discussion. Identification of mutations confirmed the genetic heterogeneity of FXI deficiency. Most of the patients with mutations did not have any bleeding complications, whereas some had severe bleeding symptoms. Genetic screening for F11 gene mutations is important to decrease the mortality and morbidity rate associated with FXI deficiency, which can be life-threatening if bleeding occurs in tissues with high fibrinolytic activity