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    CtrlAQUA Final Report

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    Effect of cold plasma generated with different gas mixtures on safety, quality and nutritional aspects of fresh sea bream fillets

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    Cold atmospheric plasma (CAP) is a non-thermal technology, successfully used to decontaminate and extend the shelf-life of various foods. However, since CAP is highly oxidative, it can cause quality deterioration in sensitive matrices, such as fish products. This research aimed to evaluate the effect of CAP treatment with a surface dielectric barrier discharge (SDBD) with different gas mixtures (80% Ar/20% O2, or 80% N2/20% O2) on the decontamination of inoculated pathogens (E.coli and L.innocua; log 4 CFU/g inoculum) and endogenous spoilage microbiota and on the main quality indices of seabream (Spaurus aurata) fillets. For selected appropriate treatments, the impact on the nutritional value of the products was investigated through in vitro digestion, bioaccessibility of fatty acids and the degree of protein hydrolysis. The use of CAP resulted in a decrease in the bacterial load in the fresh sea bream fillets up to 1 log CFU/g obtained with Ar/O2 gas mixture for 20 min, affected by the treatment duration, but not by the gas mixture. Although a slight increase in lipid oxidation was observed (from 0.5 mg MDA/kg to a maximum of 4 mg MDA/kg), the digestibility of the products was not affected. Industrial relevance From an industrial point of view, increasing shelf-life of perishable products such as fish fillets with an environmentally friendly and non-thermal technology could represent a great advantage; however, maintaining quality is of paramount importance for the industrial use of this novel processing technology. The results of the present study show negligible effects on the nutritional quality of seabream fillets, which encourages further research.publishedVersio

    Near infrared light penetration in watermelon related to internal quality evaluation

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    The interaction between near infrared (NIR) light and the sample is a key factor to take into consideration when developing applications using NIR spectroscopy. The aim of this study was to assess the penetration depth of the NIR light through both intact watermelons and the red flesh of the cut fruit, using an interactance NIR system. The results showed a maximum penetration of between 9 and 11 mm in the assays carried out on intact watermelons and 11 mm in the watermelon flesh. The prediction performance of the different NIR system configurations used were compared by quantifying the SSC in intact watermelons in a non-destructive way. The results obtained for the assay in which the illuminated regions were separated by 28 mm (Distance 4) reported the best results for determination of SSC (%) obtaining an R2cv = 0.73 and RMSECV = 0.39 % and an R2p = 0.81 and RMSEP = 0.30 % when the set of data for that distance was split into calibration and internal validation sets.publishedVersio

    What do we talk about when we talk about integration: towards a differentiated view on integration and fragmentation in coastal and marine spatial planning

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    Bob Dylan once sang that he contained multitudes. So too does integration. More integrated planning of coasts and oceans has long been hailed as a goal and is seen as a pathway towards a more legitimate, cost-efective, equitable and sustainable planning of marine space. However, a reading of the literature indicates that many integration eforts have seemingly failed to reach their potential, and there is no clear understanding of what integration means or how we should best go about achieving it. The paper claims that this uncertainty partially stems from a unnuanced and static treatment of the concept, and a lack of recognition of the multitudes of integration. The paper argues frstly that fragmentation should not uncritically be seen as the antithesis to integration and as a negative property to be avoided. Secondly, there needs to be greater recognition of both the varying degrees of integration and the contextually dependent necessity of diferent degrees of integration. Lastly, it is more fruitful to see the multitude of nodes in the expanding ‘network of planning’ not as fragmentation, but as diferentiation. Such an approach allows us to see integration as a mean towards more sustainable planning of coastal and marine areas, not end in and of itself.What do we talk about when we talk about integration: towards a differentiated view on integration and fragmentation in coastal and marine spatial planningpublishedVersio

    Evaluation of complementary numerical and visual approaches for investigating pairwise comparisons after principal component analysis

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    We propose and evaluate numerical and visual methods for investigating paired comparisons after principal component analysis (PCA). PCA results can be visualized to facilitate an understanding of the relationships between the products and the sensory attributes. But identifying and visualizing significant product differences in multiple PCs simultaneously is not straightforward. A benefit of the proposed methods is that they provide a screening tool for evaluating PCA results rapidly. We begin with a real data set which is analyzed and submitted to the truncated total bootstrap (TTB) procedure. This TTB procedure simulates and analyzes results from virtual panels. The TTB-derived results form clouds of uncertainty around each product and paired comparison. Although these clouds can be visualized directly or by plotting the smallest contours that enclose 95% of their kernel-estimated densities, we propose that plotting TTB-derived 95% confidence ellipsoids provide a less cumbersome approach. We show that it is also possible to calculate P values that evaluate whether pairs of products are discriminated in the PCA subspace. The interpretation of these P values coincides with the visual interpretation of the confidence ellipsoids. The volumes of these confidence ellipsoids, which quantify uncertainty, are calculated easily. The confidence ellipsoids, the P values, and the volumes provide a simple and consistent approach for investigating paired comparisons after PCA. We illustrate the methods with two real data sets, one a sensory quantitative-descriptive data set from a trained panel, the other a check-all-that-apply (CATA) data set from a consumer panel. We also conduct a simulation study based on each of these data sets. The results from these simulation studies show that under repetition, the 95% confidence ellipsoids often have coverage of approximately 95%, but in some cases, coverage can be substantially lower. This indicates that the proposed ellipsoids have an approximately frequentist interpretation, but coverage varies. The complementary numerical and visual approaches can be applied to a wide range of data sets from sensory evaluation and to data from other domains.submittedVersio

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