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Effects of chilled storage and cryopreservation of sperm, and sperm to egg ratio on fertilization success in lumpfish (Cyclopterus lumpus)
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Valideringsrapport – tilleggsrapport: Analyse av cystein/cystin (BIOLAB A99) ved bruk av Büchi Multivapor™ og Waters ACQUITY UPLC/Waters AccQ•Tag Ultra
Get PDFDet ble validert nytt inndampingsutstyr (Büchi MultivaporTM) og nytt instrument (Waters ACQUITY UPLC) for metoden BIOLAB A99 Cystein/cystin. Selektivitet, linearitet, robusthet, presisjon og riktighet ble validert, måleområde ble vurdert, og måleusikkerhet ble beregnet. Valideringen demonstrerte at ytelsen til metoden er god og som forventet.Valideringsrapport – tilleggsrapport: Analyse av cystein/cystin (BIOLAB A99) ved bruk av Büchi Multivapor™ og Waters ACQUITY UPLC/Waters AccQ•Tag UltrapublishedVersio
Optimized multilayer coating using layer-by-layer assembly method for excellent oxygen barrier of poly(lactic acid) based film
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PCB-126 spiked to polyethylene microplastic ingested by juvenile Atlantic cod (Gadus morhua) accumulates in liver and muscle tissues
Get PDFIn the present study, polyethylene (PE) microplastics (150–300 μm) were added to Atlantic cod (Gadus morhua) feeds at 1 %, either in their present form (Virgin PE) or spiked with PCB-126 (Spiked PE). The feeds were given to juvenile cod for a 4-week period. The fish grew from 11 to 23 g with no significant difference between dietary treatments. Cod fed spiked PE showed a significantly higher concentration of PCB-126 in liver and muscle samples compared to control and fish ingesting virgin PE. In accordance with the accumulation of PCB-126 in the liver, the expression of hepatic cyp1a was higher in cod fed spiked PE. Notably, we observed that spiked PE, as well as virgin PE, have an effect on skin. Overall changes indicated a reduced skin barrier in fish fed a diet containing PE. Indicating that PE itself through interaction with gut tissue may influence skin health in fish.PCB-126 spiked to polyethylene microplastic ingested by juvenile Atlantic cod (Gadus morhua) accumulates in liver and muscle tissuespublishedVersio
Triploid Atlantic salmon in aquaculture - Consequences for fish health and welfare under farming conditions
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Development of genomic markers associated to growth-related traits and sex determination in lumpfish (Cyclopterus lumpus)
Get PDFCleaner fish species have gained great importance in the control of sea lice, among them, lumpfish (Cyclopterus lumpus) has become one of the most popular. Lumpfish life cycle has been closed, and hatchery reproduction is now possible, however, current production is reliant on wild caught broodstock to meet the increasing demand. Selective breeding practices are called to play an important role in the successful breeding of most aquaculture species, including lumpfish. In this study we analysed a lumpfish population for the identification of genomic markers linked to production traits. Sequencing of RAD libraries allowed us to identify, 7193 informative markers within the sampled individuals. Genome wide association analysis for sex, weight, condition factor and standard length was performed. One single major QTL region was identified for sex, while nine QTL regions were detected for weight, and three QTL regions for standard length. A total of 177 SNP markers of interest (from QTL regions) and 399 high Fst SNP markers were combined in a low-density panel, useful to obtain relevant genetic information from lumpfish populations. Moreover, a robust combined subset of 29 SNP markers (10 associated to sex, 14 to weight and 18 to standard length) provided over 90% accuracy in predicting the animal's phenotype by machine learning. Overall, our findings provide significant insights into the genetic control of important traits in lumpfish and deliver important genomic resources that will facilitate the establishment of selective breeding programmes in lumpfish.publishedVersio
Differentiation and traffic of IgM<sup>+</sup> B cells between focal dark spots in skeletal muscle of Atlantic salmon, lymphoid and adipose tissues
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Calanus fnmarchicus hydrolysate improves growth performance in feeding trial with European sea bass juveniles and increases skeletal muscle growth in cell studies
Get PDFThe world will be dependent on the development of novel feed ingredients from renewable sources to ensure sustainable growth of the aquaculture industry. Zooplankton like Calanus finmarchicus are viable new raw material candidates, as they have optimal nutrient profiles for aquatic animals and may be sustainably harvested in large volumes. In this study, the aim was to investigate if a protein hydrolysate of C. finmarchicus was able to influence the growth performance of fish. The effect of dietary inclusion of hydrolysates was tested in a feeding trial with European sea bass (Dicentrarchus labrax) juveniles, benchmarking calanus hydrolysate (CH) against commercially available hydrolysates. The diet with CH inclusion yielded increased growth, with significantly higher body weight than hydrolysates of sardine and tuna fish at the end of the trial. The observed growth-promoting effects were further examined using an in vitro model with skeletal muscle cells from Atlantic salmon. Through bioactivity experiments with muscle cells grown in media containing CH, low-molecular fractions were found to have the greatest positive effect on proliferation, viability, and expression of muscle-specific genes. Characterization of the most potent fraction revealed an abundance of small peptides, along with amino acids and marine metabolites associated with increased muscle growth.publishedVersio