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Calibration transfer between different spectrometers by wavelength correspondence
In this paper we present a method for transferring calibrations between different spectrometers based on assigning wavelength correspondence. It has been tested for near-infrared (NIR) and Raman spectroscopic instruments, and three examples are included in the paper. The calibration transfer is done in three steps: first wavelength correspondence is established. Second, PLS models are built and tuned for the new spectrometer. Third, the PLS models are slope and bias corrected. The advantages with this approach are that it does not require transfer samples and that there is only one parameter to tune: the number of PLS components. While a few samples with reference values are required for the tuning, it is fewer than methods with multiple parameters that need to be tuned.publishedVersio
Effects of the Interaction between Dietary Vitamin D3 and Vitamin K3 on Growth, Skeletal Anomalies, and Expression of Bone and Calcium Metabolism-Related Genes in Juvenile Gilthead Seabream (Sparus aurata)
The interaction between vitamin D and vitamin K is crucial for regulating bone metabolism and maintaining calcium homeostasis across diverse animal species due to their complementary roles in calcium metabolism and bone health. However, research on this interaction of vitamin D and K in fish, particularly Mediterranean species like gilthead seabream, is limited or not studied. This study aimed to understand the effects of different dietary combinations of vitamin D3 and K3 on juvenile gilthead seabream. Accordingly, seabream juveniles were fed with varying combinations of vitamin D3/vitamin K3 (mg/kg diet) for 3 months: (0.07/0.01), (0.20/0.58), (0.19/1.65), (0.51/0.74), (0.56/1.00). At the end of the trial, survival, growth, body morphology, serum calcitriol, and vertebral mineral composition remained unaffected by varying vitamin levels, while gene expression patterns related to bone formation, resorption, and calcium regulation in various tissues were significantly influenced by both vitamins and their interaction. Gilthead seabream juveniles fed the 0.07/0.01 mg/kg diet upregulated calcium-regulating genes in the gills, indicating an effort to enhance calcium absorption to compensate for dietary deficiencies. Conversely, an increase in vitamin D3 and K3 up to 0.19 and 1.65 mg/kg, respectively, upregulated bone formation, bone remodeling, and calcium homeostasis-related gene expression in vertebra and other tissues. On the contrary, a dietary increase in these vitamins up to 0.56 mg/kg vitamin D3 and 1.00 mg/kg vitamin K3 downregulated calcium metabolism-related genes in tissues, suggesting an adverse interaction resulting from elevated levels of these vitamins in the diet. Hence, sustaining an equilibrium in the dietary intake of vitamin D3 and vitamin K3, in an appropriately combined form, may potentially induce interactions between the vitamins, contributing to favorable effects on bone development and calcium regulation in gilthead seabream juveniles.publishedVersio
Protein fractionation by microfiltration of goat milk: Influence on proteolysis, metabolites and sensory characteristics of cheeses produced throughout lactation
This study addresses seasonal variations in goat milk by examining the effects of microfiltration (MF) and micro- and diafiltration (MDF) in standardising goat milk composition for cheese production. The research focuses on MF and MDF treatments at two lactation stages, analysing their impact on the quality of a hard goat milk cheese. Key parameters such as total solids, pH, organic acids, free amino acids, protein profiles, and sensory properties during cheese ripening were evaluated. Sensory attributes were assessed using Quantitative Descriptive Analysis (QDA), revealing different profiles between treatments. The MF cheeses showed a balanced profile, while MDF cheeses varied more in sourness, acidity, and texture, also influenced by lactation stage.publishedVersio
Industrial CO2 capturing by mass cultivation of microalgae (diatoms): Processes, sustainability and applications
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Tracing the Reverse Relationship Among Environmental Benefits, Business Model Innovation, and Eco-Innovation: Does Cooperation Matter?
This study investigates the direct and reverse relationships among environmental benefits, eco-innovation, and business model innovation (BMI), emphasizing the role of national and foreign cooperation among firms. We employed a structural equation model using data from the 2014 European Community Innovation Survey (CIS), the most recent survey to include questions on firms' environmental activities and eco-innovations. We studied 6827 firms from the Czech Republic (2363), Germany (3264), and Greece (1200). Findings confirm reverse relationships between eco-innovation and BMI and between eco-innovation and environmental benefits. This study challenges the notion that (foreign) cooperation positively impacts eco-innovation, highlighting a paradox that warrants further investigation. Additionally, we emphasize the reliance of companies on cooperative partners for BMI. Our results underscore the need for a holistic approach to eco-innovation in light of increasing global environmental challenges. Practical implications include heightened environmental awareness, adjustments to corporate business models, and enhanced cooperation within the analyzed countries.publishedVersio
Fish dispersal from a sabotage-mediated massive escape event
Farm sabotage can cause massive fish escape events with significant ecological and socio-economic risks. This study examined the fate of Mediterranean seabass Dicentrarchus labrax escapees following a large-scale escape event caused by sabotage in the Western Mediterranean Sea. We monitored the escapee density and size structure over 3 mo after the escape at increasing distances from the escape point, up to 45 km away. Fish density adjacent to the escape location 5 d after the sabotage was, on average, 114 ± 44.7 (SE) fish per 100 m2. Our analyses showed that fish density decreased by 17% for every km away from the location, dropping to 2 and 1% after 1 and 2 mo, respectively, following the escape event. As escapee density declined throughout time and space, the size distribution of seabass shifted towards larger sizes. The rapid decrease in fish densities highlights the need for contingency plans focusing on fishing efforts in the coastal areas near the escape location (<20 km) within the first 24 h. These results are paramount to mitigating the risks associated with escape events cost-effectively. We emphasise the importance of sabotage prevention measures, such as security systems that can quickly detect intruders and trigger an immediate response to deter them. Additionally, enforcing appropriate sanctions based on the severity of the damage caused could help to discourage future sabotage attempts.publishedVersio