Pelita Perkebunan (Coffee and Cocoa Research Journal, CCRJ)
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Protein and Mineral Contents of Cocoa Beans Fermentation from Luwu Timur Regency, South Sulawesi
Get PDFCocoa beans are raw materials of cocoa processed food products rich in protein and minerals. Availability of sufficient quantities greatly determines the nutritional quality of the food. One of the post-harvest processes affecting the protein and minerals contents in cocoa beans is fermentation. The purpose of this study was to determine the protein, macro, and micronutrients contents in fermented cocoa beans of PBC 123, BR 25, and MCC 02 clones with fermentation time variations for 24, 48, 72, 96, and 120 hours. Methods of analysis used Kjeldahl and Atomic Absorption Spectrophotometer. The results showed that clones, fermentation time, and their interaction affect protein, macro and micronutrients content in cocoa beans. The interaction between BR 25 clone and 48-hour fermentation time showed the highest protein content (13.34%). The highest macronutrients content were found in PBC 123 clone with 72-hour fermentation time for Ca, in MCC 02 clone with 24-hour fermentation time for Mg, in BR 25 clone with 72-hour fermentation time for Na, and in BR 25 clone with 24-hour fermentation time for K. The highest micronutrients contents were found in PBC 123 clone with 96-hour fermentation time for Fe, in MCC 02 clone with 120-hour fermentationtime for Mn, and in BR 25 clone with 96-hour fermentation time for Zn. Variations of protein, macronutrients and micronutrients contents in cocoa beans were affected by clone variety and fermentation time treatment
Intensity of vascular streak dieback in different cocoa clones and various agro-climatic conditions
Get PDFVascular streak dieback (VSD) is one of the main diseases on cocoa. This disease can produce a heavy damage in susceptible plants. Agro-climatic condition influences the VSD disease severity level. A study on the relationship between agro-climatic condition and VSD disease severity was conducted in eight locations which were selected based on difference in agro-climatic conditions including altitude, rainfall, number of wet, and dry months. Randomized complete block design was used consisting of eight agro-climatic conditions as treatments which consisted of 200 trees samples, and scored for VSD intensity. A study was also conducted on the response of cocoa clones with different level of resistance at different altitude at Kendeng Lembu, Jatirono, Sungai Lembu, Banjarsari, and Sumber Asin Plantations. A split plot design was applied consisting of two factors. The first factor was location including Pager Gunung (highland) and Besaran (lowland). The second factor was clone resistance with two levels: PA 191 (resistant) and BL 703 (susceptible). VSD scores and stomatal characteristics (stomata number, stomata diameter, and stomata aperture) were determined. The results of experiment showed that VSD scoring differed significantly between the eight agro-climatic conditions. The highest VSD score occurred in the lowland (Gereng Rejo, Banjarsari Plantation, 38 m asl.), where the average annual rainfall was 2161 mm, with five dry months. Cocoa trees in Sumber Asin (580 m asl.), with the average annual rainfall of 2302 mm and 8.5 wet months/3.5 dry months were mostly free of VSD disease. Altitude was positively correlated with rainfall, and negatively correlated with VSD severity. Number of wet months was negatively correlated with VSD severity. Conversely, number of dry months was positively correlated with VSD. The result indicated that genotype, environment, or their interaction did not significantly affect number and aperture of stomata. Although stomatal diameter was significantly affected by environment, genotypes or their interaction with environment did not influence this character
Development of Pratylenchus coffeae in Biochar Applied Soil, Coffee Roots and Its Effect on Plant Growth
Get PDFBiochar improve physical, chemical and biological properties of soil. It may also be used as botanical pesticides. The experiment was aimed to determine the effect of biochar on population development of parasitic nematode Pratylenchus coffeae. The experiment was carried out in Nematology Laboratory and in a greenhouse of Indonesian Coffee and Cocoa Research Institute (ICCRI), Jember, Indonesia. The experiment was arranged according to completely randomized design with six treatments of biochar concentrations, i.e. 4%; 3%; 2%; 1%; 0.5% and 0% dry weight of soil. Research results revealed that biochar application of 4.0% was effectively killed 74.5% of P. coffeae, while the lowest mortality level of 37.5% at biochar 0.5% treatment. In the greenhouse trial, application of biochar 4.0% was the most effective in suppressing the population of parasitic nematode in coffee seedlings. Application of biochar was also able to increase the growth and biomass of coffee seedlings
Morphological Variation of Somatic Embryos of Coffea arabica L. During Some Sub-Culture Periods
Get PDFOne of factors that affects the success of a plant cultivation using somatic embryogenesis method is the formation of somatic embryos from embryogenesis callus. This research aimed to study the effect of sub-culture period on quality and quantity of the somatic embryos of Coffea arabica. This research used explants of somatic embryos of Arabica coffee obtained from the leaves of 2K Andungsari clone. The embryos were taken during embryogenes is callus phase using Murashige-Skoog culture media added with B5 vitamin and auxin hormone (2,4dichlorophenoxy acetic acid) 0.5 mg/L and sitokinin (benzyl amino purin) 1 mg/L. Observation on somatic embryos obtained from the sub-culture period of 3, 6, 9, 12 and 15 weeks. The parameters observed in this study included quantity and quality of the somatic embryos during each sub-culture period. Observations on quantity of the somatic embryos were conducted based on number of embryos per cluster, while quality was measured from the percentage of normal embryos, histological analysis, and morphological measurement on weight and size of the normal embryos. The result showed that the best quantity of somatic embryos was obtained from the sub-culture period of nine weeks with 18.4 somatic embryos per cluster. The best quality embryos were also obtained in the sub-culture period of nine weeks with the percentage of normal embryos 71.4%. Histological analysis carried out on the somatic embryos obtained from sub-culture of three weeks period showed that the cells of the embryos were formed by living and solid cells which nucleus were clearly seen in the center of the cell, indicating that the embryos were formed by young tissues. Data of morphological parameters showed that normal embryos during the sub-culture period 3 to 15 weeks weighed around 0.23–0.78 mg and length of around 0.18–0.25 cm. The data can be used to predict number of explants and required time to produce certain number of normal embryo
Carbon, Nitrogen, Phosphorus, and Potassium Content Partitioning of Cocoa Tree Parts in Serian, Sarawak
Get PDFMany claim that commodity plantations release carbon stock and thus contribute to climate change effects. Yet, studies on cocoa carbon stock and nutrients are limited, especially in Malaysia therefore, objective of this study was to determine if there were any significant differences of carbon stock in five different tree parts of cocoa, namely branches, main stem, main roots, fibrous roots, and leaves. Twenty cocoa trees (aged 10 years old) at Kpg Muara Ahi, Serian Sarawak were destructively sampled in March, 2016, and cut into five tree compartments prior to analysis by using CHN and AAS Analyzer. Mean comparison was carried out by using one-way ANOVA SPSS 21.0 software. Total cocoa carbon stock was 27.32 M C ha -1 which branches contributing 47% from the total carbon (12.92 Mg C ha -1 ), followed by main stem (5.42 Mg C ha -1 ), taproot (4.05 Mg C ha -1 ), fibrous root (2.49 Mg C ha -1 ) and leaves (2.44 Mg C ha -1 ). Branches and main stems contained higher total carbon stock due to high total biomass (kg), how-ever, main root showed significantly (p<0.01) highest in carbon content with 42.58% in terms of per tree parts. As for the nutrient content, leaves showed signifi-cantly(p<00.1) the highest in nitrogen, phosphorus and potassium content compared to other tree parts with N 2.55 ± 0.04 ppm; P 0.20 ± 0.006 ppm and K 2.68 ± 0.24 ppm, respectively. From the study, it showed that cocoa tree does help in storing carbon. By knowing N, P and K partitioning within cocoa tree, this enable further study to be done especially in applying fertilizer for optimum yield
Selection of Prospective Drought-Tolerant Cocoa Hybrids Based on Additive Main Effect and Multiplicative Interaction Analyses
Get PDFGlobal warming impact in increasing temperature and becomes threat for cocoa production because growth of cocoa depends on the amount of water available in the environment. The objective of this study was to describe cocoa hybrid that may potentially be developed as drought-resistant cocoa hybrid. The study used 14 types of cocoa hybrids and ICCRI 06H as control. Those plants were approximately 4 years old and the hybrids were planted in Sumber Asin Experimental Station and Kendeng Lembu Plantation. The variables being observed to evaluate 2015 El-Nino effect were percentage of growing plants, tree diameter, jorquette height, flush and flower intensity in 2016. Analysis of variance, correlation and additive main effect and multiplicative interaction (AMMI) biplot were used to find out which hybrids were potentially drought-resistant. The finding showed that there was significant interaction between the genotypes (hybrids) and environment towards the percentage of growing plants, tree diameter, flush and flower intensity. The correlation between the variables showed that there was significant, negative correlation between the percentage of growing plants and height; even though there was significant correlation between the two, it was quite low. There was significant correlation between the tree diameter and the jourquette height while there was not any between the tree diameter and the percentage of growing plants. The AMMI analysis classified the drought tolerant hybrids into two groups, (1) cocoa hybrids adaptive to both normal as well as the temperature and water scarcity (drought), was ICCRI 03 x KW 617, and (2) drought tolerance cocoa hybrids, was KW 516 x KW 617
Auto-Dissemination of Beauveria Bassiana in Controlling Coffee Berry Borer
Get PDFCoffee berry borer (CBB), Hypothenemus hampei Ferr. (Coleoptera, Scolytidae) is generally controlled by applying integrated pest management, particularly through sanitation and using biological agents. Beauveria bassiana is wellknown as an entomopathogenic fungus, which has been proven to be the most effective and prospective agent that could be developed to control CBB. Dissemination of microbial biocontrol agents via insect or predators may has advantages for safe spore dispersal to targeted pests with the added benefit of predation. The research with aim to determine if spores of B. bassiana can be picked up by CBB from disseminated containers had been conducted at Plant Protection Laboratory and Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute. Ten infected coffee berries and ten CBB adults were sprayed with various concentration of B. bassiana suspension, and then infested them in 10 healthy berries in petridish. The same trials was conducted in the field, by infested them to healthy berries on coffee branches. Observation was done on the percentage of infected berries and number of CBB infected by B. bassiana inside the berries. The results showed that spores of B. bassiana could be picked up by CBB from disseminated containers, although it was very low (2.5%). B. bassiana was not effective in suppressing CBB infestation by auto-dissemination, therefore to improve the effectiveness of B. bassiana in controlling CBB, it was advised to re-spray at least 3 times in every cropping season, especially at the flying time of CBB around 2 P.M
Effectiveness of Humic Acid Application on Growth of Coffee Seedings
Get PDFSoil fertility is an important aspect to consider in a plantation because it can affect plantation productivity. Furthermore, degradation of soil fertility issue is increasing therefore it needs innovation to improve soil fertility. Humic acid is an alternative material which can be used for improving soil fertility and supporting plantation production. This research objective was to investigate the effectivenes of humic acid for growth of coffee seedlings. This research was done in Indonesian Coffee and Cocoa Research Institute green house. Experi-ment used completely randomized design trial with two factors. First factor was the materials used humic acid, coffee pulp compost, and manure. Second factor was doses of materials: 0 g, 10 g, and 30 g per 2 kg dry soil. Research parameters used plant height, number of leaves, stem diameter, wet weight, soil carbon, N, Pavailable, K available, and pH analysis. The results showed that humic acid 30 g significant increasing plant height and K available. Furthermore, humic acid dose 10 g and 30 g resulted root, stem diameter, and leaves weight were better than compost from coffee pulp and manure. Soil carbon was increase after humic acid, compost from coffee pulp, and manure application. Humic acid, compost from coffee pulp, and manure made soil pH were stabile
Chromatographic identification of leaf color characteristics on fine-flavor and bulk cacao as selection indicator
Get PDFA problem encountered in plant breeding process to determine bean color quality of fine-flavor cocoa is a long selection period. Preliminary results indicatedthat the fine-flavor cocoa has a low color reflectance than bulk cocoa. The objectiveof this study is to find more applicable and easier method to do the early detectionof fine-flavor cocoa on the breeding population. Detection of the leaf color haracteristics was done by chromatographic and spectrophotometry analysis. hromatographic analysis was carried out in Kaliwining Experimental Station, IndonesianCoffee and Cocoa Research Institute using complete random design consistedof two types of cocoa; fine-flavor (10 clones) and bulk cocoa (10 clones). pectrophotometric analysis was conducted at Ma Chung University, Malang, East Java.Four clones of bulk cocoa and six clones of fine flavor cocoa were used in thisanalysis. The results of the study showed that the flush color characteristics of thefine-flavor cocoa were different from the bulk cocoa characteristics as shown bythe differences in the value of L*, b* and anthocyanin content. Flush characteristic s ofthe fine-flavor cocoa had brighter color, more toward green with higher yellowlevel, while the bulk cocoa had darker flush, red and lower yellow level. Thecolor parameter of L* and b* had a higher genetic variation compared with theenvironment variation. However, the parameter a* had a higher environmental variation compared with genetic variation. Grouping of fine-flavor cocoa and bulk cocoacould be done through the detection of flush anthocyanin content, when tendencyof flush anthocyanin content on fine-flavor cocoa was lower than bulk cocoa.The detection of flush color particularly L* and b* parameters through hromatographic analysis and anthocyanin content through spectrophotometric analysismay be used as a criteria for selection the fine flavor cocoa on seedling phase
Relationship Analysis Between Leaf-Stomata Characteristics with Cocoa Resistance to Vascular-Streak Dieback
Get PDFCharacteristics of leaf-stomata indicate having relationship with  the resistance of  cocoa  to  vascular-streak  dieback  (VSD)  caused  by  Ceratobasidum  theobromae. This  research  has  objective  to  identify  the  relationship  between leaf-stomata  haracteristicsto VSD resistance in order to develop criteria for selection. Trial was establised in  randomized-complete  block design  with three  blocks as replications  in  Kaliwining Experimental Station of Indonesian Coffee and Cocoa Research Institute (ICCRI) using 15  clones  which  had  different  background  on  VSD  resistance.  Leaf-stomata  of  the tested  clones  were characterized  using variables of number of stomata,  stomata size, width  of  opened-stomata  and  size  of  stomata  mouth  in  three  different  level  of  leaf growth,  namely  flush,  young  and  mature.  VSD  resistance  was  assessed  by  scoring the damage in the scale of 0-6 that  the tested clones were classified into five group sof resistance.  Analysis  of  variance  indicated  a  significant  effect  of  cocoa  clone  to  the number of  stomata  in  all categories  of leaf  sample  but the  other leaf-stomata  haracteristics  were  significantly  affected  by  cocoa  clone  just  at  the  young  and  mature leaf.  Correlation  analysis  performed  a  significant inter-correlation  between  variables of leaf-stomata characteristics in young leaf and the score of VSD resistance. However, number of stomata and width of opened-stomata in the mature leaf were significantly correlated  to  the  score  of  VSD  resistance.  Genetic  variance  analysis  showed  a  high category of broad sense heritability for the number of stomata and width of the  penedstomata  in  young  leaf  that  both  of  the  variables  can  be  developed  as  criteria  for selection on  VSD  resistance