Indonesian Center for Animal Research and Development : Scientific Journal of ICARD
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Melioidosis: Important Neglected Zoonosis and Their Presence in Animal in Indonesia, Handling Constraints and Solutions
Full text linkMelioidosis is a zoonosis caused by the bacterium Burkholderia pseudomallei, a saprophyte bacteria that is widely found in the environment. This bacteria causes Melioidosis in various animal species with clinical manifestations vary depending on species, but generally fever, depression, weight loss, respiratory sign, lameness, swelling of the joints, and potentially death. The incidence of Melioidosis in humans has been widely reported in many countries and it is estimated that every year there are 20,038 cases with a mortality rate of 51%. In contrary, there have not been many reports of Melioidosis in animals in the world, due to Melioidosis is not well known, limited diagnostic techniques and laboratory facilities in identifying the disease correctly. The presence of Melioidosis in animals and humans has been reported in Indonesia but the incidence is not yet known. This paper discusses Melioidosis in animals, etiological agent, epidemiology, modes of transmission, clinical manifestations, diagnosis and prevention and their presence in animals in Indonesia and constraints and solutions
The Role of Selenium in Controlling Reproductive Disorder in Beef Cattle
Full text linkReproductive failure is regarded as a problem in the breeding of beef cattle as indicated by low calving rate, low conception rate, longer periods of calving interval, and neonatal mortality. Selenium deficiency may lead to non-infectious reproduction disorder in ruminants. The purpose of this review is to evaluate the role of selenium in preventing reproduction failure of beef cattle. In the reproduction process, selenium is required for cellular respiration, cellular oxidation, DNA and RNA replication, the integrity of cells membrane, and releasing free radicals. Selenocysteine (SeCys) is the main component of selenoprotein consisting of glutathione peroxidase (GPX) to inhibit free radicals formation and reduce risks of tissue damage. Glutathione peroxidases (GPXs) involved are: GPX-1 (cytosolic), GPX-2 (gastrointestinal specific), GPX-3 (plasma/extracellular), GPX-4 (phospholipid-hydroperoxides/intracellular) and GPX-5 (spermatozoa mitochondrial capsule). Selenium as an antioxidant is potentially involved in reducing fertility, stillbirth, abortus, and premature in cows based on oxidative stress and selenium deficiency. In male cattle, the role of selenium is to maintain the integrity of cells membrane, spermatozoa fertility, and ejaculation. The selenium deficiency may therefore affect spermatozoa fertility, motility, and fusion to oocytes due to lipid peroxidation. It is then regarded as a factor in reproductive failure mainly in the infertility of cattle. Treatments for reproductive failure due to selenium deficiency could be conducted by supplementing selenium in the mineral mix, periodical dosing of selenium salt, and providing Se-mineral block, the combination of selenium and vitamin E or selenium supplementation in soils to improve the quality of fodder and feeds
Changes in Physiological Condition of Broiler Chickens Sprayed with Water before Transportation
Full text linkTransportation to the slaughterhouse is a series of processes that can cause high levels of stress in broilers. Heat stress will increase if the distance between the farm and the slaughterhouse is far. One of the solutions to overcome heat stress due to transportation is to do watering a few minutes before the transportation so that the broilers are still able to maintain their homeostatic conditions. This study aims to determine the effect of watering methods before transportation with different distances on the haematological, hormonal, and quality status of broiler chickens. This study was arranged based on a factorial randomized block design (RAK). A total of 54 broilers of the Cobb strain aged 35 days were divided into 2 treatments, namely; without watering (P0) and watering (P1). Chickens in each treatment were transported to the poultry slaughterhouse with 2 different distances; 30km (J1) and 60km (J2), as well as 1 control treatment (without transport) (J0). Watering is done just before the transportation process. The results showed that the transportation distance increased the yellowness value (b*) of the breast meat, decreased the brightness value (L*), and increased the pH of the broiler thigh meat (P0.05). Hematocrit values, hemoglobin levels, and concentrations of the hormone triiodothyronine (T3), other meat color components, as well as the pH of the breast meat, did not change significantly, both in terms of watering, distance traveled, and the interaction between the two (P0.05). The solution of water spraying before transportation on different distances could not restore the hematology and hormonal status, as well as the meat quality of the broiler. However, the decline in meat quality was shown in transported broilers
Physiological Responses, Performance, Behaviour, and Welfare of Garut Sheep Cared using Semi-Intensive System in Indonesia
Full text linkSheep farming in Indonesia still relies on the intensive system which is very labor-intensive, movement restrictions, and reduced quantity of sheep can be kept, developed countries, on the other hand, have evolved a semi-intensive system that may minimize the number of staff while still providing welfare to the sheep. This study aimed to compare the performance, physiological responses, behavior, and welfare of reared Garut sheep in semi-intensive management with outdoor pen and full indoor pen treatments, employing a T-test experimental design using ten replicates (n=10). This experiment used 20 ewes sheep (one-year-old) with an average body weight of 18.74±2.53 kg. This experiment found no difference in dry matter intake (gram/head/day) or average daily gain (gram/head/day) between both treatments (P0.05). When compared to ewes with full indoor pen, ewes with access to outdoor pen had better physiological status especially heart rate and respiratory rate, particularly in the morning and afternoon (P0.05). The ratio of blood N/L for ewes with an outdoor pen showed better results (P0.05), and the rearing environment also did not show any difference nor induce stress on livestock with access to outdoor pen (P0.05). Ewes' welfare increases and they become more active after being provided access to outdoor pen. In conclusion, a semi-intensive system with an outside enclosure enhances Garut ewes' performance while also improving their physiological health and welfare index fulfillment. This approach has to be promoted throughout Indonesia for better farming management
Application of Infrared Thermography as a Determinant of Sub-Clinical Mastitis in Sapera Dairy Goats
Full text linkApplication of infrared thermography (IRT) sensing results versus somatic cell count (SCC) and mastitis test reagent from Bogor Agricultural University (IPB-1) was evaluated in this study for infection detection in dairy goats with subclinical mastitis. Eight Sapera dairy goats with a 35-40 kg live weight were evaluated throughout their lactation. The parameters observed including milk production, physicochemical characteristics, SCC, IPB1, and IRT sensing in the udder. The collected data were analysed using MIXED and CORR procedures from SAS. Results showed that the physicochemical characteristic of milk (fat, non-fat solids, lactose, protein, freezing point, pH), SCC and IRT were significantly different (P0.05), especially the test results for mastitis between normal and +3. The average production of goat milk with a normal until +2 mastitis test score during lactation was 1.281±253 ml/day, while a mastitis test score of +3 was 957±250 ml/day. A positive correlation was found in both the left and right udder of IPB1-SCC (r=0.70-0.74), IPB1-IRT (r=0.70-0.71), and SCC-IRT (r=0.62-0.65). This is substantial evidence that combining IRT results with SCC and IPB1 parameters can be valuable for screening subclinical mastitis in dairy goats
A Systems Dynamic Approach for Modeling Policy of Dairy Cattle Development in Indonesia
No full textMost farmers conduct small-scale dairy farming in Indonesia, so the appropriate policies are required for its development. Problems in the development of dairy farming in Indonesia cannot be solved partially but need to be solved holistically from the upstream-downstream subsystem. Existing conditions indicate that the rate of milk consumption has not been followed by milk production, so imports are still required. This paper aims to describe a systems dynamic approach model to obtain policy recommendations for dairy farming development in Indonesia. The development of the dairy cattle population and milk production in the four periods of the strategic plan of the Ministry of Agriculture for the period 2000-2019 and the development of the dairy cattle population and milk production in Indonesia for the period 2000-2021 showed positive growth. In the same period, the share of the dairy cattle population was 98.47%, and milk production was 98.93% in Java. Problem-solving in dairy cattle development has been modeled by researchers using a systems dynamic approach. Policy recommendations for dairy farming development using a system dynamic approach: good dairy farming practices, optimizing local resources, empowering farmers and institutions, utilizing appropriate technology, controlling productive dairy cows slaughter, strengthening dairy cooperatives, training for farmers, increasing business scale, and imports of dairy cows. However, improving a holistic model from the upstream to downstream subsystems is still necessary to provide alternative policy recommendations for dairy farming development in Indonesia
Mammalian Contribution to Transmission of Schistosoma japonicum Infection in West Lore, Poso, Central Sulawesi, Indonesia
Full text linkStudies on the role of domestic animals in the transmission of schistosomiasis japonica in the West Lore Sub-district, Poso District, are still limited despite its importance as zoonosis. This study used a cross-sectional design to determine schistosomiasis prevalence in domestic mammals and identify the relative contribution of each mammalian species’ schistosomiasis transmission in the West Lore Sub-District. Fecal samples were obtained from 209 animals (seven buffaloes, 70 dogs, 44 cattle, 86 pigs, and two horses). The Danish Bilharziasis Laboratory technique was used to detect both the occurrence of S. japonicum egg in feces and the intensity of schistosomiasis infection. The examination of 1852 human fecal samples using the Kato-Katz method was carried out by the Laboratory of Schistosomiasis Lengkeka. The measurement of environmental pollution with S. japonicum eggs and the relative contribution of each species in the transmission was performed. The highest prevalence of S. japonicum infection in animals was in horses (100%; 2/2), cattle (54.55%; 24/44), and pigs (51.16%; 44/86). The prevalence in buffaloes and dogs was 28.57% (2/7) and 32.86% (23/70). Cattle (69.74%) were the main contributors to S. japonicum eggs contamination in the environment, followed by pigs (21.95%) and buffaloes (4.71%). This study reported a high prevalence of schistosomiasis in animals (45.46%), while low human schistosomiasis prevalence (0.59%)
Polymorphisms of the Thy1 (Thymocyte Differentiation Antigen 1) Gene in Candidate IPB D2 Chicken Line
No full textThe Thy1 gene is a member of the immunoglobulin superfamily, which is found in many different animals. This gene encodes a Thy1 protein (CD90) which measures 25–37 kDa. Although the function of the Thy1 gene is unknown, it is thought to play a crucial role in the immunological response, particularly in the activation of T cells. The goal of this study was to find a variation in the Thy1 gene in IPB D2 chickens that might be utilized as candidates for immunoresistance genetic markers. A total of 123 samples from IPB D2 chickens consisting of 38 samples from IPB D2 G0 (basic generation) chickens and 85 samples from IPB D2 G2 (second generation) chickens were used in this study. PCR methods and direct-DNA sequencing were used in this study. Data analysis in this study consisted of genotype frequency, allele frequency, observed heterozigosity, expected heterozygosity, and Hardy-Weinberg equilibrium. The results of the study discovered two SNPs on the intron (g.2139 CT and g.2542 AG). Four haplotypes were created by combining two SNPs. The g.2139 CTand g.2542 AG were polymorphic, but not in the Hardy-Weinberg equilibrium
Diversity of SNP c.795A>G PLAG1 Gene and its Association to Birth Weight of Bali Cattle
Full text linkPLAG1 gene is one of those that regulate growth and body size. This study aimedto look at the PLAG1 gene polymorphism and its relationship to birth weight in Bali cattle using PCR-RFLP. The total sample used was 104 samples consisting of 66 Bali cattle from BPTU-HPT Denpasar and 38 Bali cattle from BPT-HMT Serading, each of which had birth weight data. PLAG1 gene polymorphism was analyzed using PCR-RFLP and the Sac1 restriction enzyme. The genotype and allele frequencies, heterozygosity, and Hardy-Weinberg equilibrium were all examined using Popgen32. General Linear Model was used to analyze the association of SNP 795AG PLAG1 gene with birth weight in Bali cattle. Amplification of the PLAG1 gene resulted in 776 bp fragments and two alleles. The PLAG1 gene had three genotypes: AA (562 bp and 182 bp), AG (562 bp, 182 bp, and 104 bp), and GG (562 bp, 182 bp, and 104 bp). Based on the results, the PLAG1 gene in Bali cattle was polymorphic. The alleles frequency of Bali cattle wasin Hardy-Weinberg equilibrium. The SNP c.795AG PLAG1 gene genotype were associated with birth weight in Bali cattle. The A allele is a determinant of high birth weight in Bali cattle where the AG genotype has the highest birth weight
Impact of Aging on Sperm Quality of Sentul Roosters
Full text linkThis study was done to determine impact of aging on sperm quality of Sentul roosters. Materials used in this study were Sentul males aged 48, 58, 68 and 78 weeks, NaCl, eosin and aquades. Semen was collected by abdominal massage method. Completely randomized design (CRD) was applied in this study with 4 treatments in 5 repetitions. Statistical analysis showed that increasing age of rooster had a very significant effect (p0.01) on decreasing semen consistency, mass motility, spermatozoa concentration and increasing spermatozoa motility. Aging in Sentul roosters affected semen volume significantly (P0.05) but did not significantly affect (p0.05): semen pH, live spermatozoa, and spermatozoa abnormalities. Average volume of semen produced in this study ranged from 0.54-0.88ml; semen pH 6.80-7.12; mass movement score 1.60-3.00; spermatozoa concentration 2.76-4.86 x109/ml; spermatozoa motility 66-79%; Live spermatozoa 91.75-93.10%, and spermatozoa abnormalities 1.75-2.51%. Semen from Sentul males aged 48-68 weeks had a thick consistency and cloudy white in color. At the age of 78 weeks, the consistency varies from thick, medium and liquid. The color of semen in Sentul males aged 78 weeks also varied from clear white to cream. It is concluded that aging in Sentul roosters causes reproductive disorders which are characterized by low sperm quality. The best quality spermatozoa were produced by Sentul roosters aged 58-68 weeks