EKSAKTA - Berkala Ilmiah Bidang MIPA (E-Journal)
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Compound Profile of Methanol Extract of Katang-Katang Leaves (Ipomoea pes-caprae) and Evaluation of Its Antibacterial and Antioxidant Activities
Full text link Increasing antibiotic resistance has prompted the search for safer and more sustainable natural alternatives. Ipomoea pes-caprae, a coastal plant traditionally used for the treatment of wounds and inflammation, is known to be rich in secondary metabolites such as flavonoids, terpenoids, alkaloids, and phenolic acids that have potential antioxidant and antibacterial properties. This study aimed to map the metabolite profile of the methanolic extract of I. pes-caprae leaves using LC-HRMS, to analyze its relationship with antioxidant activity measured by the DPPH method, and to evaluate its antibacterial activity against Staphylococcus aureus and Escherichia coli. LC-HRMS analysis identified 406 compounds with Palmitic Acid as the dominant component (24.503%), including primary and secondary metabolite groups such as flavonoids (quercetin, rutin, kaempferol), phenolic acids (caffeic, ferulic, chlorogenic acid), terpenoids (caryophyllene oxide, lupeol), alkaloids, and phytohormones. The methanol extract showed moderate antioxidant activity (IC₅₀ = 136.71 ppm) and antibacterial activity against E. coli (inhibition zone 9.5 mm), which is thought to originate from the synergy of various secondary metabolites through free radical scavenging mechanisms and bacterial membrane damage. These findings strengthen the scientific basis for I. pes-caprae as a natural source of antioxidant and antibacterial agents for the development of natural pharmaceutical products
The Evaluation of Microplastic Reduction in Biofloc Aquaculture for Sustainable Nile Tilapia Cultivation
Full text linkSustainable aquaculture requires environments free from microplastic contamination. However, microplastics are now commonly found in aquatic systems, including fish farms, where they can accumulate in organisms and enter the food chain. This study evaluates the effectiveness of biofloc technology in reducing microplastic levels in water and Nile tilapia (Oreochromis niloticus), using ecological risk indices: Pollution Hazard Index (PHI), Pollution Load Index (PLI), and Potential Ecological Risk Index (PERI). The experiment lasted 50 days with four treatments, including polyethylene (PE) and polyethylene terephthalate (PET) exposure. Microplastic concentrations ranged from 0.12 to 0.33 particles/L, with highest accumulation in the fish esophagus (39.2 ± 6.87 particles/g). Identified polymers included PE, PVC, and PA. Risk indices showed PHI = 166.69, PLI = 1.01–1.66, and PERI = 21.49, indicating medium to high ecological risks. Results show that biofloc effectively reduces microplastic levels, making it a promising solution for sustainable aquaculture. The study highlights the need for better plastic waste management policies and stricter regulation of PVC and PET near farming areas
Synthesis and Characterization of Sodium Aluminate from Aluminum and Sodium Hydroxide
Full text linkThe synthesis of sodium aluminate from aluminum and sodium hydroxide was successfully conducted to optimize its potential as a precursor for zeolite production. Aluminum was reacted with NaOH solution under continuous stirring for five hours, with variations in pH and Al-to-NaOH molar ratio to determine optimal synthesis conditions. The highest aluminum conversion (94.33%) was achieved at pH 13.6 and an Al-to-NaOH molar ratio of 1:4. Fourier-transform infrared (FTIR) spectroscopy confirmed the formation of tetrahedral [AlO₄]⁻ units through characteristic aluminate vibrational bands at 624 and 727 cm⁻¹. X-ray diffraction (XRD) analysis revealed sharp reflections at 2θ ≈ 34.8°, corresponding to crystalline NaAlO₂ (JCPDS No. 33-1200), indicating high crystallinity and phase purity. Scanning electron microscopy coupled with energy-dispersive X-ray (SEM–EDX) analysis showed irregular plate-like crystalline particles with a near-stoichiometric Na:Al ratio (~1:1), confirming compositional homogeneity. Overall, optimized alkalinity and stoichiometry were found to be critical for producing highly crystalline and compositionally pure sodium aluminate, suggesting its suitability as a high-quality, environmentally friendly precursor for zeolite synthesis
The Effectiveness of Asystasia gangetica (L.) T. Anderson Leaf Extract as a Corrosion Inhibitor for Mild Steel in Hydrochloric Acid Medium
Full text link The inhibition effectiveness of the extract in hydrochloric acid solution was analyzed using the weight loss method at 30 °C for 7 hours to determine the corrosion rate and inhibition efficiency. The active compounds were identified through phytochemical tests, while the adsorption type was determined based on thermodynamic parameters. Characterization of surface changes on mild steel was carried out using UV-Vis spectroscopy and Field Emission Scanning Electron Microscopy (FESEM). The results revealed the presence of flavonoids, alkaloids, steroids, triterpenoids, and phenolics, which contribute to the adsorption process on the mild steel surface. The lowest corrosion rate was obtained at a concentration of 8 g/L, namely 0.43 mg/cm².h, with the highest inhibition efficiency of 73.42%. The R² value approaching 1 follows the Langmuir adsorption isotherm, with a value of -23.79, indicating that EDAG molecules are adsorbed on the mild steel surface through mixed adsorption. Surface morphology analysis showed a smoother steel surface after immersion in the inhibitor solution, indicating the formation of a protective layer. Thus, compared to previous studies, EDAG exhibits higher inhibition efficiency and demonstrates potential as an environmentally friendly corrosion inhibitor alternative
Learning and Memory Impairment in High Fat Diet Induced Obesity
Full text linkObesity is a manifestation of abnormal fat accumulation which can lead to impairment in several organs, including the brain. Neuroinflammation is considered the cause of cell death as well as reactive oxygen species in hippocampal neuron cells. It results in disturbance of memory forming process. Impaired learning and memory function affects a person's ability to carry out daily tasks and lower quality of life over time, so they should be circumvented with preventive, curative, and rehabilitative measures. The understanding of the pathological mechanisms of obesity-induced memory impairment based on the changes at molecular levels is imperative for an effective management. We collected and reviewed research articles to summarize the pathological mechanisms. Twenty studies were included in this review in terms of signaling pathway, molecular markers in brain and changes in memory and behavior pattern. It is showed that memory changes in obesity could be resulted from inflammation, impaired neurogenesis and cell senescence via various mechanisms and pathways. In conclusion, the understanding of the pathomechanisms in obesity-induced memory impairment aids to its the prevention and treatment
Application of Nipah Pseudovirus System for Development of Antibody Neutralization Assay
Full text linkNipah virus (NiV) is a type of virus that can make people and many animals very sick. It can cause serious breathing problems and brains welling. Because of how dangerous and deadly it is, the World Health Organization (WHO) sees NiV as a global healthrisk. It needs to be handled in special labs that have the highest safety measures, called Biosafety Level-4 (BSL-4) facilities. Rightnow, there isn't a good vaccine or treatment available for NiV. It could be a health risk for Indonesia since it has been found in nearby countries. Indonesia doesn't have a BSL-4 lab yet. So, we need a way to evaluation NiV vaccine that can be done in a BSL-2 lab. The NiV pseudovirus (PV NiV) has special proteins that help it attach to and enter mammal cells. It is made using a system based on HIV and includes a signal detector. This setup can help create tests to measure how well antibodies work against NiV. It can also be used to monitor infections, check community immunity, develop NiV vaccines, and research new treatments to fight NiV infections
Isolation and Characterization of Flavonoid from Mimosa pudica
Full text linkIndonesia has a rich biodiversity, including the putri malu plant (Mimosa pudica L.) which is known to have secondary metabolites with medicinal potential but has not been fully utilized. The purpose of this work is to isolate and analyze secondary metabolites from M. pudica. The aerial portions were dried and macerated in methanol before being partitioned using n-hexane, dichloromethane, and ethyl acetate solvents. The ethyl acetate extract was separated using liquid vacuum chromatography, then recrystallized and purity tested (KLT, melting point, and HPLC). The chemical structure was characterized using UV-Vis, FTIR, and NMR (1H and 13C) spectroscopy. One of the 17 fractions generated pure yellow crystals with a melting point of 242-244°C and single prominent peak on HPLC. The study revealed that the chemical was quercetin, a flavonoid with a variety of biological functions. This study demonstrates that M. pudica include active flavonoid compounds that can supportaid in the development of herbal medications based on natural ingredients. These findings lay the groundwork for additional research into its bioactivity and pharmacological potential, particularly as an antidiabetic drug and antioxidant
Molecular Pathway of Phytochemicals in Preventing Sarcopenia
Full text linkSarcopenia, the age-related decline in muscle mass and function, poses a significant global health challenge. This systematic review synthesizes evidence from preclinical studies published between 2020 and 2025 to elucidate the molecular pathways through which phytochemicals prevent sarcopenia. Following PRISMA guidelines, a systematic search of databases identified 16 eligible studies. The analysis reveals that phytochemicals, including flavonoids, polyphenols, and botanical extracts, mitigate muscle atrophy by concurrently enhancing anabolic signaling via the IGF-1/PI3K/Akt/mTOR axis, suppressing proteolysis, improving mitochondrial biogenesis, and reducing oxidative stress and inflammation. Notably, combinatorial formulations and multi-target extracts demonstrate superior efficacy. The review concludes that phytochemicals represent a promising multi-targeted strategy against sarcopenia; however, this promising potential necessitates future validation through standardized clinical trials to establish efficacy and safety in human populations
Molecular Insights into Acne Vulgaris: A Multi-Omics Approach Towards Precision Medicine
Full text linkAcne vulgaris is a common dermatological disorder that significantly impacts quality of life, yet its complex pathogenesis remains incompletely understood, contributing to the variability in clinical presentation and treatment response. This review summarizes recent advances in omics-based research on acne vulgaris and explores how these findings support the development of targeted therapy. A systematic literature search was conducted in PubMed using the keywords “acne genomic,” “acne transcriptomic,” “acne proteomic,” and “acne metabolomic.” Original research articles published in English, available in full text, and published between 2015 and 2025 were included. After screening for relevance and removing duplicates, 17 studies met the inclusion criteria. Additional relevant articles were also referenced to complement the discussion. The selected studies show that large-scale molecular analysis provides a more comprehensive understanding of the molecular mechanisms underlying acne vulgaris. These findings enable the identification of novel biomarkers, better insight into pathological pathways, and the development of more targeted therapeutic strategies. Further studies are needed to validate these findings and translate them into improved strategies for the diagnosis, prevention, and treatment of acne vulgaris
Design and Reliability Analysis of Four-Legged Jacket Type Offshore Platform in North Java Sea
Full text linkIn ideal conditions, offshore platform design follows standardized international criteria such as the American Petroleum Institute Recommended Practice 2A-LRFD (API RP2A-LRFD) to ensure structural reliability and safety. However, the real conditions in the Java Sea present unique challenges, as environmental loading patterns and regional factors may differ from those assumed in global standards. This study proposes a comprehensive solution through combined structural analysis and reliability assessment using Monte Carlo simulation methods. The urgency of this research stems from the critical need to validate and potentially adjust design standards for regional applications, ensuring the long-term safety and reliability of offshore structures in Southeast Asian waters. The research objectives focus on evaluating the structural reliability of a four-legged jacket type platform using both deterministic and probabilistic approaches, specifically assessing the applicability of API RP2A-LRFD criteria to Java Sea conditions. Results demonstrate that while the structure meets basic design criteria, the reliability indices (β = 16.70 for LRFD, β = 22.29 for unfactored) suggest current load factors may be overly conservative for regional conditions