Chung Hwa University of Medical Technology
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The Role of Mst3 ( Mammalian Ste20 Kinase 3) in Renal Tubulogenesis
No full text研究領域:基礎醫學類, 生物技術
計畫編號:NSC97-2311-B273-001-MY3[[abstract]]腎臟發育時，分枝管狀形成的受損，是幼童常見的腎臟衰竭的原因。分枝管狀之形成與細胞之爬行，凋亡及生長週期有關。以HGF 刺激腎臟細胞MDCK，可使其發育成分枝的管狀。當HGF 刺激MDCK，COX-2 蛋白質的表現明顯上升。在缺乏COX-2 的老鼠中，腎臟的分枝過程會受損。另外，腎臟受傷復原時，HGF 接受器，COX-2 以及fibronectin 均會上升。若MDCK 不分泌fibronectin 則管狀分枝無法形成。屬Ste 20 激酶家族的MST3，其蛋白的功能被報導 (1)可促進細胞凋亡 (2) MST3 能控制生長週期 (3) MST3 能抑制細胞移動。這3 項功能均與分化的過程有關。加上我們發現 (i) 在老鼠出生後的腎臟中，MST3 活性上升。(ii) MST3 只有表現在腎臟的細上升枝。(iii) MDCK 大量表現MST3 時， COX-2 明顯上升。 (iv) 必須將細胞培養在舖有fibronectin 之培養皿上，當MDCK 細胞表現大量 MST3 時，才會使細胞移動速度變慢，顯示MST3 與fibronectin 有關。故我們選擇研究MST3 對腎臟發育管狀分枝形成之影響。 In vivo，我們將比較表現活化態MST3 的轉殖性老鼠與野生型MST3 的老鼠，找出MST3 在不同發育時期，在分枝管狀形成的過程，是否有所改變。In vitro，我們將會利用大量表現野生型MST3 及dominant negative MST3 之MDCK 細胞株，研究MST3 是否可經由COX-2 上升，再配合MST3 可增加fibronectin 的分泌而使而促進腎臟分枝發育。再回到in vivo 中，解出MST3 否是經由COX-2 及 fibronectin，控制腎臟分枝管狀發育的詳細機轉。
Disruption of renal branching tubulogenesis during kidney development resulted in renal dysplasia, the major cause of renal failure in young children. The morphogenetic steps are controlled by apoptosis, cell cycle and cell motility. An in vitro model in which HGF induces branching morphogenesis in MDCK epithelial cell culture in three-dimensional collagen gels is established. The COX-2 mRNA and protein expression in MDCK cells are apparently increased after stimulation with HGF. Mouse mutation of COX-2 gene resulted in defects in renal morphogenesis. Besides HGF and COX-2, the interaction of extracelluar matrix (ECM) and cells was also the regulator of branching tubulogenesis. COX-2, HGF receptor, and fibronectin are all increased when mouse was administrated with renal injury. The secretion of fibronectin by MDCK cells promoted renal tubulogenesis. Without the secretion of fibronectin, the tubule could not be formed. The Ste20 family consists of MST1-4 (mammalian Ste20 kinase 1-4)。It was reported that (1) MST3 can promote apoptosis. (2) MST3 can regulate cell cycle and (3) MST3 can inhibit migration. Our results showed (i) the activity of MST3 was elevated in the kidney of 1-2 week old rat (ii) MST3 was exclusively expressed at the thin ascending limb of the kidney. (iii) COX-2 was upregulated in MST3 overexpressed MDCK. (iv) The migration inhibition function of MST3 was appeared when fibronectin was served as the matrix. In vivo, we will compare the active HA-MST3 expressing transgenic mice with wild type MST3 mice to find the effect of MST3 on renal tubulogenesis. In vitro, we will proof that MST3 can stimulate COX-2 to control HGF-induced renal tubulogenesis. Furthermore, HA-MST3 MDCK and dominant negative MST3 will be used to answer if MST3 can secrete fibronectin to lead the formation of renal tubulogenesis. Finally, in vivo and in vitro evidences regarding the relationship between COX-2, fibronectin and MST3 will be considered, rendering new insight into regulating roles for MST3 in renal tubulogenesis
Establishment of Environmental Friendly Bioremediation Approaches for Solving Residual Hydrocarbon Problems
No full text研究領域:環保工程
計畫編號:NSC98-2221-E273-001-MY
Waveguide Device Analyses and Applications of the Tunable Phononic Crystal
No full text研究領域:電子電機工程類
計畫編號:NSC96-2221-E273-009-MY3[[abstract]]在本計畫提案中，將以二維之聲子晶體結構所產生之能隙現象為基礎，並配合智能材料的特性，作為依據來討論系統的波傳導特性，並利用智能材料所具有的主動控制特性，設計出具主動可調式之聲子晶體結構系統，並利用點、線缺陷的設計概念，用以發展新型的聲波之波導元件設計。首先將以二維週期結構之理論分析模型為基礎，利用數值分析方法，發展出一套完整的理論分析模式，緊接著將利用智能材料本身所具有的主動可調式之材料特性，依據理論分析的結果，將兩者做一統合設計應用。後續將利用已知的模擬分析軟體，根據主動控制之概念，並加入點、線缺陷的設計，設計出一系列可作為主動控制聲波能隙的元件設計(如共振腔、波導之設計)，冀望能夠建立一系列完整的分析模型、實驗系統與設計規範。在元件的設計應用方面，本計畫首先將以高效率及高準度的聲波濾波器設計為主，並輔以智能材料的配合應用，設計出能夠以聲波、電能、光能與熱能加以控制的新型聲波元件。在熟悉完美週期結構的聲子晶體應用後，將配合點、線缺陷的設計概念，設計出新型的共振腔、超音波波導與微波波導等元件，甚至類似以聲光調變的應用設計等等的統合應用設計，在未來一系列的研究當中也能夠達成。相信此計畫若能成功運作，將可以使各類的智能材料更進一步使用於工業設計應用上，也能夠使相關之聲波方面的設計應用有更進一步的實現與成長。
In the proposal, characteristics of the wave propagations of the selective twodimensional phononic crystals will be studied and applied to design a new type of devices according to the characteristics of the band gaps of the two-dimensional phononic crystals and combining the particular material properties of the common smart materials. Additionally, the smart materials are used to be as tunable units on the periodic system and the characteristics of the wave propagations of the system are investigated in this study. First, the characteristics of the phononic crystals are investigated and a detailed analytic model will be developed to study the band structures in this phononic system. Then, the characteristics of the smart materials, such as piezoelectric, electro-rheological fluid (ER), magneto-rheological fluid (MR), shape memory alloy (SMA), elastomers will be studied and also applied to combine the smart materials with the periodic system. Then, the point or linear defect is added into the tunable phononic crystal to act as a cavity or waveguide device. According to the above investigations, we can design a series tunable and active control acoustic device. Finally, we can design some new types of devices and more convenient applications on the acoustic wave and it can be applied to investigate other tunable ultrasonic devices in future studied according to the results of this proposal. This novel application about the phononic crystals and smart materials is helpful to the wave propagation development
Comparison of Effects of One-Time and Multiple---Time Eating Disorders Prevention Programs
No full text研究領域:教育學
計畫編號:NSC98-2410-H273-002[[abstract]]本計畫乃延續96、97 年國科會專題計畫（NSC-96-2413-H-273-002、 NSC-97-2410-H-273-004）之研究成果。前述研究已証實「飲食異常預防方案」之有效性，但在招募過程，忙碌的大專生卻常因方案週數太多而無法參與，故本研究嘗試將方案濃縮為單次，比較單次方案（6 小時）與多次方案（連續四週，1.5 小時/週）在飲食異常預防效果的差異。研究對象設定為兩所大專院校之飲食異常高危險群女學生，每校成立「單次方案組」與「多次方案組」各5 組、每組6-8 人之團體，總收案人數約120-160 人；在方案介入後，比較兩組在方案結束當時、三個月與六個月於「飲食態度量表-26 題」、「身體意象量表」及「中文版暴食問卷」的得分差異。團體結束後亦將針對改變顯著與不顯著之參與者共60 位，進行深入之質性訪談與分析，以暸解此兩種執行方式之有效性與限制性。本計劃成果可暸解兩種形式之飲食異常預防方案之立即與延續性效果，有助於未來更彈性地選擇適用在各級校園中的方案執行方式，以降低飲食異常問題之發展與嚴重性。
This is an extended study of previous projects in 1996 (project no.: NSC-96-2413-H-273-002) and 1997 (project no.: NSC-97-2410-H-273-004). These two projects have proven the effectiveness of the Prevention Program for Eating Disorders. But during the recruitment process, busy college students were not able to participate because the program took too many times. Therefore, this present research tries to compress several sessions into a single event, and see the differences between one-time (6 hours) program and multiple-time (1.5 hours/week for 4 weeks) program in the effect of preventing eating disorders. Targeted subjects are set to high-risk female students of eating disorders at two colleges. Each school will have 5 one-time groups and 5 multiple-time groups, with 6-8 participants in each group. The total number of participants will be 120-160. Scoring differences will be compared among three inventories— EAT-26, MBSRQ, and BITE-Chinese version at the end of session, and at 3- and 6-month follow-ups. In-depth qualitative interviews and analyses will also be conducted to 60 participants after intervention to comprehend the effectiveness and limitation of these two types of programs. Being able to understand the prompt effect and continuity of the prevention program of eating disorders, results of this research will help the planners select applicable programs to all kinds of schools to reduce the development and the seriousness of eating disorders
