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Implementing Student Evaluation of Teaching in the University of Science and Tachnology;A Case Study
No full textExperiences and Demands of Dental Service of Complete Denture Wesrers - A Grounded Theory Application
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Effects of Multimdia Compact Dise of Exercise on Low Back Pain,Low Back Disability and Sleep Quality in Primary Caregivers
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Study on the Antioxidation Property of Osmanthus fragrans Flower in BALB/c mice and human, and Anti-Hearing Loss Effect of Noise-Exposed workers.
No full text目錄 I
圖目錄 IV
表目錄 VI
誌謝 VII
中文摘要 IX
ABSTRACT XI
縮寫對照表 XIII
第一章 緒論 - 1 -
第一節 研究背景與動機 - 1 -
一、 桂花背景介紹 - 1 -
二、 桂花之多酚化合物 - 2 -
第二節 研究動機 - 10 -
第二章 文獻回顧 - 13 -
第一節 噪音的定義 - 13 -
第二節 噪音與聽力損失的相關性 - 13 -
第三節 噪音與自由基 - 15 -
第四節 噪音與抗氧化酵素機制 - 17 -
第五節 抗氧化防禦系統 - 21 -
一、 抗氧化防禦系統與氧自由基 - 21 -
二、 體內之抗氧化防禦系統 - 22 -
第三章 研究方法 - 28 -
第一節 桂花成份分析 - 28 -
第二節 桂花 75 %乙醇萃取物對於 BALB/C 小鼠抗氧化能力之評估 - 32 -
第三節 桂花多酚飲品提升抗氧化能力的評估 - 39 -
第四節 不同劑量之桂花膠囊對人體抗氧化能力之評估 - 45 -
第五節 桂花膠囊對噪音性聽力損傷之預防研究 - 49 -
第四章 結果與討論 - 55 -
第一節 桂花之總多酚、類黃酮及抗氧化指標成份分析 - 55 -
第二節 桂花75%乙醇萃取物對BALB/C小鼠抗氧化能力評估 - 58 -
一、桂花75 %乙醇萃取物對BALB/c小鼠各臟器之總抗氧化能力之影響 - 58 -
二、桂花75 %乙醇萃取物對BALB/c小鼠各臟器之GSH含量之影響 - 58 -
三、桂花75 % 乙醇萃取物對BALB/c小鼠各臟器之TBARs含量之影響 - 59 -
第三節 飲用桂花茶對於提升人體抗氧化能力之評估 - 65 -
一、 受試者血漿中 glutathione (GSH) 之含量 - 65 -
二、 受試者血漿中總抗氧化能力 (ORAC) 測定 - 65 -
三、 受試者血漿中 superoxide dismutase (SOD) 之活性 - 66 -
四、 受試者血漿中 glutathione peroxidase (GPx) 之活性 - 66 -
第四節 不同劑量桂花膠囊對人體抗氧化能力評估 - 71 -
一、 桂花粉末之安全劑量 - 71 -
二、 血漿中總抗氧化能力 (ORAC) 之含量 - 71 -
三、 血漿中 GSH 之含量 - 72 -
四、 安全性評估 - 72 -
第五節 噪音性聽力損傷與服用桂花膠囊後抗氧化能力之相關性研究 - 67 -
一、 所得均能音壓結果測定 - 67 -
二、 廠內環境噪音類型測定 - 67 -
三、 三條產線音壓強度分析 - 70 -
四、 個人劑量分析 - 71 -
五、 純音聽力檢查結果 - 72 -
六、 4000Hz 暫時性聽力損失之影響 - 73 -
七、 血漿中提升總抗氧化能力 (ORAC) 之影響 - 74 -
八、 血漿中提升 GSH 含量之影響 - 76 -
九、 血漿中 GPx 活性之影響 - 77 -
十、 血漿中 GSSG 含量之影響 - 77 -
十一、 血漿中SOD活性之影響 - 78 -
十二、 抗氧化項目與 4000Hz 暫時性聽力損失之相關係數 - 79 -
第五章 結論 - 104 -
第六章 參考文獻 - 105 -
附錄 - 118 -
附錄一、受試者同意書 - 119 -
附錄二、飲食建議表 - 122 -
附錄三、動物與人體的每公斤體重劑量拆算係數表 - 123 -
附錄四、美國十大職業病和傷害 - 124 -
參考文獻
林俊德 (1991)。噪音與健康。工業安全衛生月刊。24：52-55。
林麗梅 (2004)。廢紙回收再製廠勞工噪音暴露對聽力與 DNA 的損傷。
中國醫藥大學環境醫學研究所碩士論文。
林政佑、蔡朋枝 (2009)。噪音性聽力損失與抗氧化基因易感性的相關性，
以及其可能的藥物治療。工業安全科技 68，16-20。
林雅玲 (2003)。探討台灣鄉土蔬菜 紅甘藷葉中多酚類之生體可利用率
及對人體抗氧化狀態之影響抗氧化狀態。台北醫學大學保健營養學
系碩士論文。
呂峰洲 (2000)。神奇抗氧化劑：免疫乳漿蛋白濃縮物。台北。元氣齋
出版社。
郭芳榕 (2004)。台灣產桂花抗氧化成分及其生理活性之研究。中華醫
事學院生物科技研究所碩士論文
趙文婉、張珍田、周淑姿（2002）。淺談植物類化學物質對抗自由基之
機制。食品工業 34(2)，49-59。
洪千雅、蘇正德 (1994)。槐花芸香苷之分離，純化及抗氧化性之研究，
東海學報 35，243-258。
游錫榕、陳建志 (2000)。白珠藤之成份研究。中醫藥雜誌11(3)，129-133。
劉紹興 (1994)。台灣職業病面面觀與職業醫學之發展。中華職業醫學雜
誌 1(2) : 16-20。
勞研所 (1994)。勞工作業環境安全衛生狀況認知調查。台北：行政院勞
工委員會勞工安全衛生研究所。
黃福龍 (2007)。台灣產桂花之安全性、抗致敏性氣喘及護肝功能之評
估。中華醫事科技大學生物科技研究所碩士論文。
柯慧珊 (2008)。桂花萃取物對 OVA 致敏小鼠腦部氧化傷害的保護作用。中華醫事科技大學生物科技研究所碩士論文。
郭肇凱、顏國欽、許輔、張隆仁 (2006)。山防風水萃物對小鼠體內免疫調節之研究。台中區農業改良場研究彙報 90，41-50。
石東生、陳秋蓉 (2008)。噪音暴露勞工服用抗氧化藥物對噪音引起的聽
覺影響。行政院勞工委員會勞工安全衛生研究所。
史雅中 (2004)。補充葡萄籽萃取物對馬拉松運動所導致氧化壓力之影響。國立體育學院運動科學研究所碩士論文。
Abbate, C., Concetto, G., Fortunato, M. O. (2005). Influence of environmental factors on the evolution of industrial noise-induced hearing loss. Environ Monit Assess 107, 351-61.
Akerboom, T. P. and Sies, H. (1994). Transport of glutathione disulfide and glutathione S-conjugates in hepatocyte plasma membrane vesicles. Methods in Enzymology 233, 416-425.
Andrea, G., Fausta, N., Alessia, G., Luigi, M., Paolo, F., and Scaccini, C. (2000). Beer increases plasma antioxidant capacity in humans. J Nutr Biochem 11, 76-80.
Brand-Williams, W., Cuvelier, M.E., and Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology 28, 25-30.
Calabro, M. L., Tommasini, S., Danato, P., Stancanelli, R., Raneri, D., Catania, D., Costa, C., Villari, V., Ficarra, P., and Ficarra, R. (2005). The rutin/²- cyclodextrin interactions in fully aqueous solution: spectroscopic studies and biological assays. J. Pharm. Biomed. Anal., Amsterdam 36 (5) : 1019-1027.
Cao, G., Booth, S.L., Sadowski, J.A., and Prior, R.L. (1998). Increases in human plasma antioxidant capacity after consumption of controlled diets high in fruit and vegetables. Am J Clin Nutr 68, 7.
Carlsson, P.I., Van Laer, L., and Borg E. (2005). The influence of genetic variation in oxidative stress genes on human noise susceptibility. Hear 202, 87-96.
Cheng, C. C., Yang, M. H., Wen, H. M. AND Cherm, J. C. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10(3), 178-182.
Chimi, H., Cillard, J., Cillard, P., and Rahmani, M. (1991). Peroxyl and hydroxyl radical scavenging activity of some natural phenolic antioxidants. J Am Oil Chem Soc 68, 307- 312.
Christov, A., Ottman, J.T., and Grammas, P. (2004). Vascular infla- mmatory, oxidative and protease-based processes: implications for neuronal cell death in Alzheimer's disease. Neurol Res 26, 540-546.
Chung, K. Y., Lee, S. J., Chung S. M., Lee, M. Y., Bae O.N., and Chung, J. H. (2005). Generation of free radical by interaction of iron with thiols in human plasma and its possible significance. Thrombosis Research 116, 157-164.
Cross, C. E. (1997). General biological consequences of inhaled enviro- nmental toxicology. In The Lung: Scientific Foundations,2421. Raven Press, Philaphia.
Daniela, E., Patrizia, R., Alessandra, B., Paola, F., Pier, L. and Biagi, G. T. (2005). Effectiveness of moderate green tea consumption on antioxidative status and plasma lipid profile in humans. Journal of Nutritional Biochemistry 16, 144– 149.
Deepak M, and Handa SS. (2000). Antiinflammatory activity and chemical composition of extracts of Verbena officinalis. Phytother Res 14, 463-5.
Derekoy, F. S., Dundar, Y., Aslan, R., Cangal A. (2001). Influence of noise exposure on antioxidant system and TEOAEs in rabbits. Eur Arch Otorhinolaryngol 258, 518-22.
Diaz, A.M., Abad, M.J., Fernandez, L., Recuero, C., Villaescusa, L., Silvan, A.M., and Bermejo, P. (2000). In vitro anti-inflammatory acti- vity of iridoids and triterpenoid compounds isolated from Phillyrea- latifolia L. Biol Pharm Bull 23, 1307-1313.
Emara, A.M., and El-Bahrawy, H. (2008). Green tea attenuates benzene- induced oxidative stress in pump workers. J Immunotoxicol 5, 69-80.
Esterbauer, H., Dieber-Rotheneder, M., Striegl, G., and Waeg, G. (1991). Role of vitamin E in preventing the oxidation of low-density lipoprotein. Am J Clin Nutr 53, 314S-321S.
Fetoni, A. R., Piacentini, R., Fiorita, A., Paludetti, G., Troiani, D., (2009).
Water-soluble coenzyme Q10 formulation (Q-ter) promotes outer hair cell
survival in a guinea pig model of noise induced hearing loss (NIHL). Brain Res 1257, 108–116.
Fortunato, G., Marciano, E., Zarrilli, F. (2004). Paraoxonase and superoxide
dismutase gene polymorphisms and noise-induced hearing loss. Clin Chem 50, 2012-2018.
Fridovich, I. (1998). The trail to superoxide dismutase. Protein Sci 7, 2688-2690.
Gao, J. J., Igalashi, K.; Nukina, M., (1999). Radical scavenging activity of phenylpropanoid glycosides in Caryopteris incana. Bioscience Biotechnology & Biochemistry, 983-988.
Greene, M. (1995). Musician’s health issues often overlooked. Billboard 107, 8.
Halliwell, B., Gutteridge, J. M., and Cross, C. E. (1992). Free radicals, anti- oxidants, and human disease: where are we now? J Lab Clin Med 119, 598-620.
Halliwell, B., Gutteridge, J., 1999. Free Radicals in Biology and Disease.
Oxford University Press, Oxford.
Hamid R. Zare, Reza Samimi, M. Mazloum Ardakani, (2009). A Comparison of the Electrochemical Behavior of Rutin at an Inactivated, Activated, and Multi Wall Carbon Nanotubes Modified Glassy Carbon Electrode. Int. J. Electrochem. Sci 4, 730 – 739.
He Z. D., But P. P. H., Chan T. W., Dong H., Xu H. X., Lau C. P., Sun H. D., (2001). Antioxidative glucosides from the fruits of Ligustrum lucidum. Chem Pharm Bull 49(6) , 780-4.
Heffner, J. E., and Repine, J. E. (1989). Pulmonary strategies of antioxidant defense. Am Rev Respir Dis 140, 531-554.
Henderson, D., Bielefeld, E. C., Harris, and K. C. (2006). The role of oxidative stress in noise-induced hearing loss. Ear. Hear 27, 1-19.
Henderson, D., McFadden, S. L., Liu, C. C., (1999). The role of antioxidants
in protection from impulse noise. Acad. Sci. 884, 368-380.
Hertog, M. G., Feskens, E. J., Hollman, P. C., Katan, M. B., Kromhout, D. (1993). Dietary antioxidant flavonoids and risk of coronary heart disease:the Zutphen Eldarly Study. Lancet .342, 1007-1011.
Hiroaki, O., Akiko S., and Nobuo S. (2001). Plasma total glutathione concentrations in healthy pediatric and adult subjects. Clinica Chimica Acta 312, 227–229.
Hollman, P. C., van Trijp, J. M., Mengelers, M. J., de Vries , J. H., Katan, M.
B. (1997) Bioavailbility of the dietary antioxidant flavonol quercetin in man. Cancer Lett 114, 139-140.
Jacono, A. A., Hu, B., Kopke, R.D. (1998). Changes in cochlear antioxidant enzyme activity after sound conditioning and noise exposure in the chinchilla. Hear Res 117, 31-38.
Janbaz, Khalid H., Saeed, Sheikh A., Gilani, Anwar H., (2002). Protective
effect of rutin on paracetamol- and CCl4-induced hepatotoxicity in
rodents. Fitoterapia. 73, 7-8, 557-563.
Ingold, K. U., Webb, A. C., Witter, D., Burton, G. W., Metcalfe, T. A., and Muller, D. P. (1987). Vitamin E remains the major lipid-soluble, chain -breaking antioxidant in human plasma even in individuals suffering severe vitamin E deficiency. Arch Biochem Biophys 259, 224-225.
Jones, C. (1996). Occupational hearing loss and vibration induced disorders. British Medical Journal 313, 223-226.
Janse van Rensburg, C., Erasmus, E., Loots, D.T., Oosthuizen, W., Jerling, J.C., Kruger, H.S., Louw, R., Brits, M., and van der Westhuizen, F.H. (2005). Rosa roxburghii supplementation in a controlled feeding study increases plasma antioxidant capacity and glutathione redox state. Eur J Nutr 44, 452-457.
Keaney, J. R. and Frei, B., (1994). Antioxidant protection of low-density lipoprotein and its role in the prevention of atherosclerotic vascular disease. In”Nature Antioxidants in Human Hearth and Disease.” Ed by B. Frei, pp. 303-351. Academic Press: San Diego, CA.
Kim, K. H., Lee, K. W., Kim, D. Y., Park, H. H., Kwon, I. B., Lee, H. J., (2005). Optimal recovery of high-purity rutin crystals from the whole plant of Fagopyrum esculentum Moench (buckwheat) by extraction, fractionation and recrystalization. Bioresour. Technol 96 (15), 1709-1712.
Kitts, D. D. (1997). An evaluation of the multiple effects of the anti- oxidant vitamins.Trends Food Sci. Tech.8, 198-203.
La Casa, C., Villegas, I., Alarcon de la Lastra, C., Motilva, V., and Martin Calero, M.J. (2000). Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharmacol 71, 45-53
Lanningham-Foster, L., Chen, C., Chance, D. S., Loo, G. (1995). Grape
Extract inhibits lipid peroxidation of human low density lipoprotein. Biol Pharm Bull 18, 1347-1351.
Lautermann, J., Crann, S. A., McLaren, J. (1997). Glutathione - dependent antioxidant systems in the mammalian inner ear: effects of aging ototoxic drugs and noise. Hear Res 114, 75-82.
Lenaz, G., Fato, R., Formiggini, G., Genova, M.L., (2007). The role of Coenzyme Q in mitochondrial electron transport. Mitochondrion 7 Suppl, S8–33.
Lenzi, P., Frenzilli, G., Gesi, M., Ferrucci, M., Lazzeri, G., Fornai, F., Nigro, M. (2003). DNA damage associated with ultrastructural alterations in rat myocardium after loud noise exposure. Environ Health Perspect 111, 467-71.
Li, J. X., Xin, D., Li, H., Lu, J. F., Tong, C. W., Gao, J. N., Chan, K. M. (1999). Effect of verbascoside on decreasing concentration of oxygen free radicals and lipid peroxidation in skeletal muscle" Chung-Kuo Yao Li Hsueh Pao — Acta Pharmacologica Sinica pp. 126-130.
Liao, Y. H., Houghton P. J. and Hoult J. R., (1999). Novel and Known Constituents from Buddleja Species and Their Activity against Leukocyte Eicosanoid Generation. Journal of Natural Products 62 (9), 1241-1245.
Lin, C. Y., Wu J. L., Shih, T. S., Tsai, P. J., Sun, Y. M., Mag, M. C., and
Guo, Y. L. (2010). N-Acetyl-cysteine against noise-induced temporary
threshold shift in male workers. Hearing Research, 1-6.
Funes, L., Laporta, O., Calero, M. C., Micol, V., (2010). Effects of verbascoside, a phenylpropanoid glycoside from lemon verbena, on phospholipid model membranes. Chemistry and Physics of Lipids 163, 190–199.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem 193, 265-275.
Lynch, E. D., Kil, J. (2005). Compounds for the prevention and treatment of
noise-induced hearing loss. D.D.T. 10, 1291-1298.
Lu, Y. and Foo,L. Y. (2000). Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem 68, 81-85.
Jacob, R. A. （1995）. The integrated antioxidant system. International Journal for Vitamin and Nutrition Research 15, 755-766.
Ma, S. C., HE, Z. D., DENG, X. L., Paul P. H. B., Vincent E. C. O., XU H. X., Spencer H. S. L., and LEE S. F. (2001). In vitro evaluation of secoiridoid glucosides from the Fruits of Ligustrum lucidum as antiviral agents. Chem. Pharm. Bull. 49(11), 1471 - 1473.
Maki, K., Hayame, N., Shinsuke, H., Akiharu W. and Kunihisa S. (2007). Blood superoxide dismutase (SOD) decrease following oral admin- istration of plant SOD to healthy subjects. Journal of Health 53(5), 608-614.
Martinez-Cayuela, M. (1995). Oxygen free radicals and human disease. Biochimie 77, 147-161.
McFadden, S. L., Ohlemiller, K. K., Ding, D., (2001). The influence of superoxide dismutase and glutathione peroxidase deficiencies on noise-
Induced hearing loss in mice. Noise Health 3, 49-64.
Meister, A. (1983). Selective modification of glutathione metabolism. Science 220, 472-477.
Meister, A. (1994). Glutathione-ascorbic acid antioxidant system in animals. J Biol Chem 269, 9397-9400.
Michelle M., Louise L. and Paul L. (2007). Red wine consumption increases
antioxidant status and decreases oxidative stress in the circulation of both
young and old humans. Nutrition Journal, 1-8.
Morata, T. C., Dunn, D. E., Sieber, W. K., (1994). Occupational exposure to noise and ototoxic organic solvents. Arch. Environ. Health. 49, 359-365.
Morrissey, P. A., and O'Brien, N. M. (1998). Dietary Antioxidants in Health and Disease. International Dairy Journal 8, 463-472.
Mortazavi, S., Kashani, M. M., Khavanin, A., Alameh, A., Mirzaee, R., Akbari, M. (2010). Effects of N-Acetylcysteine on auditory brainstem response threshold shift in rabbits exposed to noise and carbon monoxide. American Journal of Applied Sciences 7 (2), 201-207.
Moskaug, J. O., Carlsen, H., Myhrstad, M. C., and Blomhoff, R. (2005). Poly- phenols and glutathione synthesis regulation. Am J Clin Nutr 81, 277S-283S
Naczk, M., and Shahidi, F. (2004). Extraction and analysis of phenolics in food. J Chromatogr A 1054, 95-111.
Nazaroglu, N.K., Sepici-Dincel, A., and Altan, N. (2007). The effects of sulfonylurea glyburide on superoxide dismutase, catalase, and gluta- thione peroxidase activities in the brain tissue of streptozotocin-induced diabetic rat. J Diabetes Complications.
Nuttall, S. L., Martin, U., Sinclair, A. J., and Kendall, M. J. (1998). Glutathione: in sickness and in health. Lancet 351, 645-646.
Ostrakhovitch, Elena A. a; Afanas’ev, Igor B. b, 2001. Oxidative stress in rheumatoid arthritis leukocytes: suppression by rutin and other antioxidants and chelators , Biochemical Pharmacology62(6), 743-746.
Ostrachovich, E.A.a; Korkina, L.G.a; Abramova, N.E.a; Afanas'ev, I.B.aO., (1995). Effect of Rutin on Oxygen Radical Production in Normal and Iron-overloaded Rats., Toxicology Letters 78, 63.
Ohlemiller, K. K., McFadden, S. L., Ding, D. L., Flood, D. G., Reaume, A. G., Hoffman, E. K. (1999). Targeted deletion of the cytosolic Cu/Zn- su- peroxide dismutase gene (Sod1) increases susceptibility to noise-induced hearing loss. Audiol Neurootol 4, 237-246.
Pannala, A. S., Singh, S. and Rice-Evans, C., (1999). Flavonoids as pero- xynitrite scavengers In Vitro. In "Methods in Enzymology: Oxidants and antioxidants 299, 207-235.
Papucci, L., Schiavone, N., Witort, E., Donnini, M., Lapucci, A., Tempestini, A., Formigli, L., Zecchi-Orlandini, S., Orlandini, G., Carella, G., Brancato, R., Capaccioli, S., (2003). Coenzyme Q10 prevents apoptosis by inhibiting mitochondrial depolarization independently of its free radical scavenging property. Biol. Chem. 278, 8220–8228.
Park, S. Y., Bok, S. H., Jeon, S. M., Park, Y. B., Lee, S. J., Jeong, T. S. and Choi, M. S. (2002). Effect of rutin and tannic acid supplements on cholesterol metabolism in rats. Nutrition Research 22(3), 283-295.
Pennacchio, M., Syah, Y.M., Ghisalberti, E.L., and Alexander, E. (1996). Cardioactive compounds from Eremophila species. J Ethnopharmacol 53, 21-27.
Pinar A., Ihsan C., Nursen B. and A. Ahmet Basaran, (2002). In vitro immunomodulatory activity of verbascoside from Nepeta ucrainica L. Phytother. Res 16, 593–595.
Rabinowitz, P.M., Pierce, W. J. and Hur, M. (2002). Antioxidant status and hearing function in noiseexposed workers. Hear Res 173, 164-171.
Ravindran, R., Devi, R. S., Samson, J., Senthilvelan, M. (2005). Noise- stress- induced brain neurotransmitter changes and the effect of ocimum sanctum (Linn) treatment in albino rats. J. Pharmacol Sci 98, 354-60.
Renaud S. and de Lorgeril M. (1992). Wine, alcohol, platelets, and the french
paradox for coronary heart disease. Lancet 339(8808), 1523-1526.
Rice-Evans, C.A., Miller, N.J., and Paganga, G. (1996). Structure-anti- oxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 20, 933-956.
Rice-Evans, C. (2004). Flavonoids and isoflavones: absorption, metab- olism, and bioactivity. Free Radic Biol Med 36, 827-828.
Saint-Cricq De Gaulejac, N., Provost, C., and Vivas, N. (1999). Comp arative study of polyphenol scavenging activities assessed by different methods. J Agric Food Chem 47, 425-431.
Schoneich, C. (1999). Reactive oxygen species and biological aging: a mechanistic approach. Exp Gerontol 34, 19-34.
Schulz, J.B., Lindenau, J., Seyfried, J., and Dichgans, J. (2000). Gluta thione, oxidative stress and neurodegeneration. Eur J Biochem 267, 4904-4911.
Sheng, G.Q., Zhang, J.R., Pu, X.P., Ma, J., and Li, C.L. (2002). Protective effect of verbascoside on 1-methyl-4-phenylpyridinium ion-induced- neurotoxicity in PC12 cells. Eur J Pharmacol 451, 119-124.
Shimada K., Fujikawa K., Yahara K. and Nakamura T., (1992). Autioxidative properties of xanthan on the autoxidation of soybean oil in cycodextrin emulsion. J.Agic. Food Chem. 40(6): 945-948.
Staal, F.J., Ela, S.W., Roederer, M., Anderson, M.T., and Herzenberg, L.A. (1992). Glutathione deficiency and human immunodeficiency- virus infection. Lancet 339, 909-912.
Stansfeld, S. A., Matheson, M. P. (2003). Noise pollution: non-auditory effects on health. Br Med Bull 68, 243-57.
Taga, M. S., Miller, E. E. and Pratt, D. E., (1984). Chia seeds as a source of natural lipid antioxidants. J. Am. Oil Chem. Soc. 61, 928-931
Terashvili, M., Pratt, P.F., Gebremedhin, D., Narayanan, J., and Harder, D.R. (2006). Reactive oxygen species cerebral autoregulation in health and disease. Pediatr Clin North Am 53, 1029-1037, xi.
Wang, P., Kang, J., Zheng, R., Yang, Z., Lu, J., Gao, J., and Jia, Z. (1996). Scavenging effects of phenylpropanoid glycosides from Pedicularis on superoxide anion and hydroxyl radical by the spin trapping method(95)02255-4. Biochem Pharmacol 51, 687-691.
Webster, R. P., Gawde, M. D., Bhattacharya, R. K. (1996). Protective effect of rutin, a flavonol glycoside, on the carcinogen-induced DNA damage and repair enzymes in rats. cancer letter 109(1-2), 185-191.
Wettasinghe, M. and Shahidi, F. (2000). Scavenging of reactive-oxygen species and DPPH free radicals by extracts of borage and evening primrose meals. Food Chem 70, 17-26.
Woisky, R. and Salatino, A. (1998). Analysis of propolis:some parameters and procedures for chemical quality control. J. Apic. Res 37, 99-105.
Woochang, L., Won-Ki M., Sail, C., Yong-Wha, L. , Hyosoon, P., Do Hoon, L., You K.L., and Ji E.S., (2005). Long-term effects of green tea ingestion on atherosclerotic biological marker in smoker. Clinical Biochemistry 38, 84-87.
Xiong, Q., Kadota, S., Tani, T., and Namba, T. (1996). Antioxidative effects of phenylethanoids from Cistanche deserticola. Biol Pharm Bull 19, 1580-1585.[[abstract]]本研究目的在探討桂花萃取物提高生物體內抗氧化能力，提高體內麩胱甘肽的效果，並進而達到噪音聽損下的抗聽損之功能性。在第一部分，以動物模式來探討桂花 75 % 乙醇萃取物的抗氧化功效。採用 4 週 BALB/c 小鼠，分成控制組 (C) 及桂花高劑量組 (HKFE)，管餵 14 天後，發現 HKFE 能有效提高肝、脾、肺、腎及腦部的總抗氧化能力及 GSH 含量，並降低脂質過氧化之功效。第二部分，進行人體實驗，探討以 1 g 及 2 g 桂花所浸泡之桂花茶對於人體抗氧化之功效，結果顯示 2 g 桂花茶飲用 7 天，即可提體內總抗氧化能力及 GSH 含量。第三部分，將桂花製成不同劑量之桂花膠囊，將受試者分成 控制組、100 mg 組、200 mg 組、300 mg 組、400 mg 及 500 mg。服用 60 天後，結果顯示，服用 400 mg 桂花膠囊組在服用 2 個月後總抗氧化能力提升 7.71 ± 1.51 %， GSH 含量提升 14.61 ± 2.19 % 。而 500 mg 桂花膠囊之受試者在服用 2 個月後總抗氧化能力提升 8.62 ± 1.50 %，GSH 含量則提升 17.36 ± 3.62 % 。因此 400 mg 及 500 mg 桂花膠囊服用 60 天後之效果接近於飲用 2 g 桂花茶 1 個星期。由於前三部分研究顯示發現，桂花萃取物能有效提升抗氧化能力之效果，因此，第四部分針對南部某啤酒廠員工進行抗聽損及抗氧化之研究。給予 24 位勞工不同配方之抗氧化保健食品，隨機分成控制組、單方組及複方組，服用 270 天。結果顯示，在廠內環境噪音均能音壓均超過 90 dBA 之噪音作業場所，勞工之純音聽力檢查發現，複方組受試者服用 270 天後，4000 Hz 暫時性聽力損失有效降低 (p < 0.01)。而在抗氧化分析研究結果顯示，複方組受試者能有效提高體內之總抗氧化能力、GSH 含量及 GPx 活性；降低 GSSG 含量及 SOD 活性。因此，推論桂花是能提高抗氧化能力，進而有效減緩暫時性聽力損失。
Sweet osmanthus contains many anti-oxidative constituents. This study evaluated the antioxidant capacity and the content of GSH of Osmanthus fragrans flower extract in vivo, and effectiveness evaluation of Osmanthus fragrans flower extract for anti-hearing loss. In part 1, effect of oral administration the Osmanthus fragrans of 75 % ethanol extract for 14 days in BALB/c mice. The BALB/c mice were divided into two groups: control group (C) and 1g/kg B.W. group (HKFE), the result found significant increases total antioxidant capacity and significant decreases TBARs on liver, spleen, lung, kidney and brain. In part 2, effects of antioxidant 1g and 2g Osmanthus drinks in vivo. The results showed that increasing the total antioxidant capacity and GSH level in human with the consumption of 2g Osmanthus drinks for 7 days. In part 3, effect of the different doses Osmanthus fragrans capsule comsumption after 60 days, the results show consumption of 400mg Osmanthus capsules after 60 days taking to enhance the total antioxidant capacity and GSH level that equivalent of drinking of 2g (high dose group; HD) Osmanthus drinks after 7 days. T