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Cyclic Step Potentiometry of Dye Sensitized Solar Cells
No full textA dye sensitized solar cell was created using dye extracted from raspberries, a TiO2 semiconductor paste, and an iodine electrolyte solution for the purpose of performing cyclic step chronopotentiometry. A voltage was applied and removed to observe how the voltage across the solar cell rose and decayed. The decays were fit to a biexponential curve and the constants were compared between the various trials to analyze trends between the time the voltage is on and off, a light being shown or not, 30 μA and 30 mA, and a electrolyte solution with or without lithium. The biexponential demonstrated how there are lifetimes for electrons diffusing out of the solar cell on the scale of seconds
Bridgewater College, Students baking, probably early 1950s
No full textArchival photograph from Bridgewater College Special Collectionshttps://digitalcommons.bridgewater.edu/family_and_consumer_sciences/1008/thumbnail.jp
Bridgewater College, Paul Yoder Jr. (photographer), Students passing a serving dish during a candlelit meal, early 1950s
No full textArchival photograph from Bridgewater College Special Collectionshttps://digitalcommons.bridgewater.edu/family_and_consumer_sciences/1018/thumbnail.jp
Cloning and Overexpression of a Bacteriophage Endolysin Gene in Escherichia coli
No full textEndolysins are enzymes used by bacteriophages (bacterial viruses) at the end of their replication cycle to degrade the peptidoglycan layer of their host bacterium from within the cell. This action weakens the integrity of the bacterial cell wall, resulting in lysis and release of the newly synthesized bacteriophages. Research suggests that endolysins could be used in place of antibiotics to fight bacterial infections in humans by targeting disease-causing bacteria without harming desirable bacteria or human cells, circumventing some of the problems with antibiotic therapy. A bacteriophage specific to Streptomyces sp. SFB5A, Brock, was previously isolated and its genome sequenced. Its genome contained two potential endolysin genes. Our goal was to clone and overexpress one of these genes, DNAM5_40, in Escherichia coli, purify the endolysin, and evaluate its activity against bacterial cell walls. We successfully cloned the E. coli DH5α transformants into E. coli BL21(DE3)RIL but were unable to get the protein to express despite conducting a temperature study, inducing with different IPTG concentrations, and trying several different media
Bridgewater College, Football game, probably 1970s
No full textArchival photograph from Bridgewater College Special Collectionshttps://digitalcommons.bridgewater.edu/football_photos/1059/thumbnail.jp
Bridgewater College, Bob Anderson (photographer), Football players, early 1970s
No full textArchival photograph from Bridgewater College Special Collectionshttps://digitalcommons.bridgewater.edu/football_photos/1050/thumbnail.jp
Bridgewater College, Football action photograph featuring Neal Hudson, early 1970s
No full textArchival photograph from Bridgewater College Special Collectionshttps://digitalcommons.bridgewater.edu/football_photos/1046/thumbnail.jp
Characterization of Streptomyces nymphaeiformis sp. nov., and Its Taxonomic Relatedness to Other Polyhydroxybutyrate-Degrading Streptomycetes
Get PDFA polyhydroxybutyrate (PHB)-degrading actinomycete, strain SFB5AT, was identified as a species of Streptomyces based on its membrane fatty acid profile and the presence of LL-diaminopimelic acid in the cell wall. It formed sporulating mycelia on most agar media, but flat or wrinkled, moist colonies on trypticase soy agar. Spores were smooth, cylindrical, and borne on long, straight to flexuous chains. It produced a light brown diffusible pigment, but not melanin. Comparison of genomic digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values indicated that strain SFB5AT was related to Streptomyces litmocidini JCM 4394T , Streptomyces vietnamensis GIMV4.0001T , Streptomyces nashvillensis JCM 4498T and Streptomyces tanashiensis JCM 4086T , plus 11 other species. However, the dDDH and ANI values were well below the species differentiation thresholds of \u3c70 and \u3c95%, respectively; also, multilocus sequence analysis distances exceeded the species threshold of 0.007. Moreover, strain SFB5AT differed from the other species in pigmentation and its ability to catabolize arabinose. Strain SFB5AT and 11 of its 15 closest relatives degraded PHB and have genes for extracellular, short-chain-length denatured polyhydroxyalkanoate depolymerases. These enzymes from strain SFB5AT and its closest relatives had a type 1 catalytic domain structure, while those from other relatives had a type 2 structure, which differs from type one in the position of a consensus histidine in the active site. Thus, phenotypic and genotypic differences suggest that strain SFB5AT represents a new species of Streptomyces, for which we propose the name Streptomyces nymphaeiformis sp. nov. The type strain is SFB5AT (=NRRL B-65520T =DSM 112030T )