1,727,602 research outputs found
The crystal structure of the Y140F mutant of ADP-L-glycero-D-manno-heptose 6-epimerase bound to ADP-beta-D-mannose suggests a one base mechanism
No full textSupported by Wellcome Trust grant 081862/Z/06/ZBacteria synthesize a wide array of unusual carbohydrate molecules, which they use in a variety of ways. The carbohydrate L-glycero-D-manno-heptose is an important component of lipopolysaccharide and is synthesized in a complex series of enzymatic steps. One step involves the epimerization at the C6 '' position converting ADP-D-glycero-D-manno-heptose into ADP-L-glycero-D-manno-heptose. The enzyme responsible is a member of the short chain dehydrogenase superfamily, known as ADP-L-glycero-D-manno-heptose 6-epimerase (AGME). The structure of the enzyme was known but the arrangement of the catalytic site with respect to the substrate is unclear. We now report the structure of AGME bound to a substrate mimic, ADP-beta-D-mannose, which has the same stereochemical configuration as the substrate. The complex identifies the key residues and allows mechanistic insight into this novel enzyme.Peer reviewe
A manno-oligoszacharidok izolálása nyersélesztőből
No full textA szénhidrátok általános jellemzése, azon belül pedig a manno-oligoszacharidok, konkrétan a mannobióz és mannotrióz kinyerése nyersélesztőbőlgjBiomérnökBSc/B
Il Modello Standard Supersimmetrico Non Minimale
No full texttesi di laurea quadriennale di Elisa Manno. Relatore Claudio Coriano', pp12
Il marxismo come teoria contro natura, ovvero come pedagogia. L'approdo in terra Karoli del Personalismo critico
No full textIl contributo cerca di illuminare le fonti marxiane della teoresi di Mario Manno
Meta-symplectic geometry of 3rd order Monge-Ampère equations and their characteristics
No full textThis paper is a natural companion of [Alekseevsky D.V., Alonso Blanco R., Manno G., Pugliese F., Ann. Inst. Fourier (Grenoble) 62 (2012), 497-524, arXiv:1003.5177], generalising its perspectives and results to the context of third-order (2D) Monge-Ampère equations, by using the so-called ''meta-symplectic structure'' associated with the 8D prolongation M⁽¹⁾ of a 5D contact manifold M. We write down a geometric definition of a third-order Monge-Ampère equation in terms of a (class of) differential two-form on M⁽¹⁾. In particular, the equations corresponding to decomposable forms admit a simple description in terms of certain three-dimensional distributions, which are made from the characteristics of the original equations. We conclude the paper with a study of the intermediate integrals of these special Monge-Ampère equations, herewith called of Goursat type.This paper is a contribution to the Special Issue on Analytical Mechanics and Dif ferential Geometry in honour
of Sergio Benenti. The full collection is available at http://www.emis.de/journals/SIGMA/Benenti.html.
The authors wish to express their gratitude towards the anonymous referees whose comments
contributed to shape the paper into its final form. The authors thank C. Ciliberto, E. Ferapontov
and F. Russo for stimulating discussions. The research of the first author has been partially
supported by the project “Finanziamento giovani studiosi – Metriche proiettivamente equivalenti,
equazioni di Monge–Amp`ere e sistemi integrabili”, University of Padova 2013–2015, by
the project “FIR (Futuro in Ricerca) 2013 – Geometria delle equazioni dif ferenziali”. The
research of the second author has been partially supported by the Marie Sk lodowska–Curie
Action No 654721 “GEOGRAL”, by the University of Salerno, and by the project P201/12/G028
of the Czech Republic Grant Agency (GA CR). Both the authors are members of G.N.S.A.G.A. ˇ
of I.N.d.A.M
Specious Art Count Matrix for Developing Mouse Brain (La Manno et al 2020)
No full textLog-normalized count matrix for dev_all.loom from La Manno et al 2020
Premio per tesi di laurea sul tema della disabilità 2018, per tesi discusse nell' A.A. 2017-2018 conferito dall'Ateneo di Roma "La Sapienza"
No full textLa tesi è stata premiata il 6 dicembre 2019 a seguito della partecipazione al bando promosso dall'Ateneo "Sapienza" per tesi che affrontano tematiche sulla disabilità discusse nell' A.A. 2017-2018. Oggetto della tesi è la progettazione condotta secondo l'approccio tecnologico al progetto, dalla fattibilità alla fase esecutiva mettendo in risalto i contenuti metaprogettuali di un servizio assistenziale semiresidenziale del tutto innovativo rivolto all'assistenza di adolescenti affetti da disturbi psichiatrici
High-throughput virtual screening and molecular dynamics simulation reveals NPC170742 a novel chalconoid compound as a potential inhibitor of D-glycero-D-manno-heptose-1,7-bisphosphate 7-phosphatase in <i>Helicobacter pylori</i>
No full textHelicobacter pylori is a gram negative spiral shaped bacteria that causes peptic ulcer and gastric cancer. It Is the sixth most prevalent cancer in the world and the third leading cause of cancer death. The increase in reported cases of H. pylori resistance to the drugs and antibiotics shows the need for the development of new and efficient drugs against the pathogen. In the present study, D-glycero-D-manno-heptose-1,7-bisphosphate 7-phosphatase (GmhB), an enzyme involved in the biosynthesis of lipopolysaccharides that encourages bacterial adherence, self-aggregation and identifying the host cells was modelled and the active sites were predicted through POCASA which is an automated ligand binding site prediction server. Natural product activity and species source (NPASS) is a database of 96,481 natural compounds that were subjected to virtual screening workflow that includes Qikprop, Lipinski rule, filtering out reactive functional groups followed by high throughput virtual screening and the top 10 compounds were selected for further induced fit docking along with the substrate D-glycero-β-D-manno-heptose 1,7-bisphosphate. The compound NPC170742 (Alpha, Beta, 3,4,5,2′,4′,6′-Octahydroxy dihydrochalcone) showed higher affinity than the substrate, and both the substrate D-glycero-β-D-manno-heptose 1,7-bisphosphate and the compound NPC170742 were subjected to molecular dynamics simulation. The results exposed the compound NPC170742 could be a potential lead compound against the enzyme D-glycero-D-manno-heptose-1,7-bisphosphate 7-phosphatase of H. pylori. Communicated by Ramaswamy H. Sarma</p
Highly Diastereoselective 1,4-Addition of an Organocuprate to Methyl α-d-Gluco-, α-d-Manno-, or α-d-Galactopyranosides Tethering an α,β-Unsaturated Ester. Novel Asymmetric Access to β-C-Substituted Butanoic Acids
No full textThe 1,4-addition of magnesium divinylcuprate prepared from vinylmagnesium bromide and cuprous
bromide to some 4-O-crotonyl derivatives of methyl α-d-glucopyranoside proceeds with a high level
of diastereochemical induction, providing the adduct in good-to-excellent yields. Other organocuprates also serve as effective carbon nucleophiles for the 1,4-addition. Removal of the carbohydrate
moiety from each adduct afforded a variety of β-C-substituted butanoic esters in remarkable
enantiomeric excess. The 1,4-addition of the same cuprate to some methyl α-d-manno- or α-d-galactopyranosidic substrates in which a crotonyl group was incorporated, each at 3-OH, was also
investigated. The reverse π-facial attack of the cuprate was observed when some d-manno-type
substrates were subjected to 1,4-addition conditions similar to those used for the d-gluco-type
substrates. Furthermore, some d-galacto-type substrates provided 1,4-adducts with higher diastereoselectivities
- …
