38 research outputs found
Proses dan Tahapan Recruitment Crew Kapal oleh PT. TJS Marine Indonesia
No full textThis final assignment aims to find out the process and stages of ship crew recruitment by PT. TJS MARINE INDONESIA. The problems that will be discussed in this final assigment focus on the causes of obstacles faced in the implementation of ship crew recruitment mechanisme to facilitate crewing management and what efforts are being made to overcome the obstacles faced. This research was carried out from April to Mei 2024 and the place where this reearch was carriet out was PT. TJS MARINE INDONESIA. This research is descrptive in nature with a qualitative approach. The method used to obtain the required information from related partie is that the author prepares observation, interview and documentation methods to obtain accurate and comprehensive data.Analysis of problems in recruitment and evaluation of the recruitment process implemented by PT. TJS MARINE INDONESIA is used as input.or recommendation for improvements and improvement strategis to increase the efficiency and quality of ship crew recruitment carried out by the compan
Photoperiod- and Triiodothyronine-Dependent Regulation of Reproductive Neuropeptides, Proinflammatory Cytokines, and Peripheral Physiology in Siberian Hamsters (Phodopus sungorus).
No full textAcknowledgements The study hypotheses and design were conceived by TJS. RB and TJS conducted, collected, and analyzed the data. RB, MD, and TJS wrote the paper. This work was financed by the University of Aberdeen College of Life Sciences and Medicine fund to TJS. We thank Elisabetta Tolla for her technical assistance. Conflict of Interest Statement The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. Funding The author(s) received no financial support for the research, authorship, and/or publication of this article.Peer reviewe
Prolactin and glucocorticoid signaling induces lactation-specific tight junctions concurrent with beta-casein expression in mammary epithelial cells
No full textAlveolar mammary epithelial cells (MECs) in mammary glands are highly specialized cells that produce milk for suckling infants. Alveolar MECs also form less permeable tight junctions (TJs) to prevent the leakage of milk components after parturition. In the formation process of less permeable TJs, MECs show a selective downregulation of Cldn4 and a localization change of Cldn3. To investigate what induces less permeable TJs through these compositional changes in Cldns, we focused on two lactogenesis-related hormones: prolactin (Prl) and glucocorticoids. Prl caused a downregulation of Cldn3 and Cldn4 with the formation of leaky TJs in MECs in vitro. Prl-treated MECs also showed low beta-casein expression with the activation of STAT5 signaling. By contrast, dexamethasone (Dex), a glucocorticoid analogue, upregulated Cldn3 and Cldn4, concurrent with the formation of less permeable TJs and the activation of glucocorticoid signaling without the expression of beta-casein. Cotreatment with Prl and Dex induced the selective downregulation of Cldn4 and the concentration of Cldn3 in the region of Tjs concurrent with less permeable TJ formation and high beta-casein expression. The inhibition of Prl secretion by bromocriptine in lactating mice induced the upregulation of Cldn3 and Cldn4 concurrent with the downregulation of milk production. These results indicate that the coactivation of Prl and glucocorticoid signaling induces lactation-specific less permeable TJs concurrent with lactogenesis
Structural stability and energetics of grain boundary triple junctions in face centered cubic materials
No full textabstract: We present a systematic study to elucidate the role of triple junctions (TJs) and their constituent grain boundaries on the structural stability of nanocrystalline materials. Using atomistic simulations along with the nudge elastic band calculations, we explored the atomic structural and thermodynamic properties of TJs in three different fcc materials. We found that the magnitude of excess energy at a TJ was directly related to the atomic density of the metal. Further, the vacancy binding and migration energetics in the vicinity of the TJ were examined as they play a crucial role in the structural stability of NC materials. The resolved line tension which takes into account the stress buildup at the TJ was found to be a good measure in predicting the vacancy binding tendency near the TJ. The activation energy for vacancy migration along the TJ was directly correlated with the measured excess energy. Finally, we show that the resistance for vacancy diffusion increased for TJs with larger excess stored energy and the defect mobility at some TJs is slower than their constituent GBs. Hence, our results have general implications on the diffusional process in NC materials and provide new insight into stabilizing NC materials with tailored TJs.The final version of this article, as published in Scientific Reports, can be viewed online at: https://www.nature.com/articles/srep0869
Diversity and community composition of methanogenic archaea in the rumen of Scottish upland sheep assessed by different methods
No full textAcknowledgments We thank Bob Mayes and Dave Hamilton of the James Hutton Institute for their permission and help in sampling the sheep digesta. Gillian Campbell and Pauline Young provided an excellent DNA sequencing service. We also thank Dr Matthew McCabe for preparing V6–V8 amplicon libraries. Author Contributions Conceived and designed the experiments: RJW. Performed the experiments: RJW BG NM SMW CJC. Analyzed the data: TJS MW SMW CJC RJW. Contributed reagents/materials/analysis tools: NM RJW MW SMW CJC. Contributed to the writing of the manuscript: TJS MW SMW CJC RJW.Peer reviewe
HGF and the regulation of tight junctions in human prostate cancer cells
No full textHepatocyte growth factor (HGF) may impact the metastasis of prostate cancer via its action on prostate stem cells or their progeny. Tight junctions (TJs) are crucial to the process of metastasis and have been previously shown to be regulated by HGF. The present study aimed to evaluate the effect of HGF on the function of TJs in human prostate epithelial, prostate stem cell-like and prostate cancer cell lines. Four human prostate cancer cell lines (PC-3, DU-145, PZHPV-7, CaHPV-10), normal adult prostate parental epithelial cells (RWPE-1) and a stem cell-like derivative of RWPE-1 (WPE-STEM) were used to assess HGF-induced changes in TJs. A significant difference was noted in the behaviour between the WPE-STEM, RWPE-1 and the cancer cell lines which was HGF concentration-dependent. However, in the WPE-STEM cells, the effect was biphasic, with the cells seemingly resistant to HGF-modulated Ti disruption. Closer examination revealed that HGF affected the redistribution of ZO-1, ZO-2 and ZO-3 away from the TJs of confluent cells with concurrent loss of claudin-1 and claudin-5, and western blot analysis revealed a loss in Ti protein expression of ZO-1 and ZO-2. We demonstrated for the first time that HGF regulates TJ function in human prostate cells. Moreover, this regulation was dependent on the tumourigenicity of the cells, with the most aggressive cells most susceptible and the stem cell-like cells least susceptible. These data offer an intriguing glimpse of how TJs affect the behaviour of prostate cancer cells and how HGF modulates the expression and function of the molecules maintaining Ti structure and function.OncologySCI(E)[email protected]
Examining a new automobile global manufacturing system Advances in logistics, operations, and management science (ALOMS) book series./ Kakuro Amasaka.
No full textIncludes bibliographical references and index."In this book, the author predicts the form of next generation automobile manufacturing, presenting a "New Automobile Global Manufacturing System" (NAGMS) that contains the hardware system with five core elements - "TDS, TPS, TMS, TIS and TJS" (Total Development System, Total Production System, Total Marketing System, Total Intelligence Management System and Total Job Quality Management System) for transforming management technology into automobile management strategy-Surpassing JIT"--1 online resource (xiii, 504 pages)
Endothelial Tight Junctions Are Opened in Cholinergic-Evoked Salivation In Vivo
No full textBlood vessels provide the original supplies for the formation of primary saliva, which is regulated by the tight junctions (TJs) between endothelial cells. Previous studies have shown that blood flow increases with vasodilatation during cholinergic-evoked salivation. However, changes in vascular paracellular permeability and the role of endothelial TJs in salivation are unknown. Here, we established an in vivo paracellular permeability detection system and observed that the endothelial TJs were permeable to 4-kDa fluorescein isothiocyanate (FITC)-dextran while impermeable to 40- and 70-kDa FITC-dextran under an unstimulated condition in mouse submandibular glands (SMGs). Pilocarpine increased the flux of 4- and 40-kDa FITC-dextran out of blood vessels but did not affect 70-kDa FITC-dextran. Claudin 5, a TJ protein specifically localized in salivary endothelial cells, was redistributed from the apicolateral membranes to the lateral and basolateral membranes and cytoplasm in cholinergic-stimulated mouse SMGs and freshly cultured human SMG tissues. In the transplanted SMGs from epiphora patients, we found that claudin 5 was present in the basolateral membranes and cytoplasm, instead of the apical region in control SMGs. Moreover, the level of phospho-myosin light chain 2 increased within the blood vessels of the pilocarpine-stimulated mouse SMGs and transplanted human SMGs, while the downstream molecule F-actin was reorganized in the endothelial cells of the transplanted human SMGs. Taken together, our findings provide direct visual evidence that the opening of endothelial TJs and the redistribution of claudin 5 are essential events contributing to cholinergic-evoked salivation, thus enriching our understanding of the secretory mechanisms that link blood flow to primary saliva formation by regulating the endothelial paracellular permeability.National Natural Science Foundation of China [81300893, 81470756]; Leading Academic Discipline Project of Beijing Education Bureau [BMU20110254]; Youth Talent Support Program from the China Association for Science and TechnologySCI(E)ARTICLE5562-5709
Évoluer vers les principes régulateurs au moyen de la thérapie du jeu synergique
No full textChildren generally do not possess the complex, expressive language skills needed to communicate the struggles they are experiencing. In response to this, a variety of play therapy models have emerged. This article concentrates on the application of a research-informed model of play therapy delivery called synergetic play therapy (SPT), which combines interpersonal neurobiology, attachment theory, nervous system regulatory principles, mindfulness, physics, and the self of the therapist. By combining this model with child-centred play therapy (CCPT), the author draws on two case study examples to demonstrate the efficacy of the SPT model when it is coupled with CCPT. The findings and case studies suggest that this approach reduces the severity of identified behavioural concerns. Future investigations in this area are recommended given the gap in the literature regarding combining SPT and CCPT.En règle générale, les enfants ne possèdent pas les compétences linguistiques complexes et expressives qu’il faut pour communiquer les luttes intérieures qu’ils éprouvent. C’est pour combler cette lacune que divers modèles de thérapie par le jeu ont vu le jour. Le présent article est centré sur l’application d’un modèle inspiré par la recherche portant sur la prestation d’une thérapie par le jeu et appelée thérapie par le jeu synergique (TJS), qui conjugue des éléments de neurobiologie interpersonnelle, de la théorie de l’attachement, des principes régulateurs du système nerveux, de la pleine conscience et du Soi du thérapeute. En associant ce modèle à la thérapie par le jeu centrée sur l’enfant (TJCE), l’auteure s’inspire de deux exemples de cas à l’étude pour démontrer l’efficacité du modèle TJS utilisé en conjonction avec la TJCE. Les résultats et les études de cas semblent indiquer que cette approche réduit la gravité des problématiques comportementales décelées. On recommande d’effectuer de plus amples analyses dans ce domaine étant donné la pénurie de données sur la combinaison de la TJS et de la TJCE
Comparison of direct return and birth-death return receivers.
No full textThe performance of frame synchronized communication systems is governed largely by the design chosen for the receiver. Some systems employ a structure in which the receiver returns directly to the sync phase when a valid marker is encountered while in the loss verify state. These have been greatly studied. In some systems, the return path in the loss verify phase is implemented as a birth-death process. These systems have not received much attention. In this report, a receiver which employs a birth-death like structure in the loss path is investigated. Expressions for the performance parameters are derived and compared with those obtained for direct return systems. It is found that both types of receivers have the same performance at lower error rates and low loss verify number, M. At high error rates and high values of M, however, the birth-death receiver outperforms the direct-return receiver. To the knowledge of the author, this is the first time that performance expressions have been reported in this form.
Tanzania Journal of Science Volume 27 (2001), pp. 59-7
