3,315 research outputs found
Clustering and dynamics of cytochrome bd-I complexes in the Escherichia coli plasma membrane in vivo.
No full textThe cytochrome bd-I complex of Escherichia coli is a respiratory terminal oxidase and an integral component of the cytoplasmic membrane. As with other respiratory components, the organization and dynamics of this complex in living membranes is unknown. We set out to visualize the distribution and dynamics of this complex in vivo. By exchanging cydB for cydB-gfpgcn4 on the E. coli chromosome, we produced a strain (YTL01) that expresses functional GFP-tagged cytochrome bd-I terminal oxidase complexes under wild-type genetic control. We imaged live YTL01 cells using video-rate epifluorescence and total internal reflection fluorescence (TIRF) microscopy in combination with fluorescence recovery after photobleaching (FRAP) and saw mobile spots of GFP fluorescence in plasma membranes. Numbers of GFP molecules per spot were quantified by step-wise photobleaching giving a broad distribution with a mean of approximately 76, indicating that cytochrome bd-I is concentrated in mobile patches in the E. coli plasma membrane. We hypothesize that respiration occurs in mobile membrane patches which we call 'respirazones'
Sub-categories of CRSWD in BD patients.
No full textA total of 27 patients met the criteria for delayed sleep-wake phase disorder, 6 patients met the criteria for non-24-hour sleep-wake rhythm disorder, and 2 patients met the criteria for irregular sleep-wake rhythm disorder. CRSWD = circadian rhythm sleep-wake disorder. BD = bipolar disorder.</p
HILT : a terminology mapping service with a DDC spine
No full textThe role of DDC in the ongoing HILT (High-level Thesaurus) project is discussed. A phased initiative, funded by JISC in the UK, HILT addresses an issue of likely interest to anyone serving users wishing to cross-search or cross-browse groups of networked information services, whether at regional, national or international level - the problem of subject-based retrieval from multiple sources using different subject schemes for resource description. Although all three phases of HILT to date are covered, the primary concern is with the subject interoperability solution piloted in phase II, and with the use of DDC as a spine in that approach
Sleep-Wake Patterns of Adolescents with Borderline Personality Disorder and Bipolar Disorder
No full textInternational audienceSleep-wake patterns are rarely examined in adolescents with borderline personality disorder (BPD) or bipolar disorder (BD). Within a developmental perspective, this study explores the sleep-wake cycle of adolescents aged 12-17 years with BPD or BD and healthy controls (HC) during periods with and without entrainment by school/work schedules. Eighteen euthymic BPD, six euthymic BD, and 20 HC adolescents wore wrist actigraphy during nine consecutive days to assess sleep-wake patterns. During school/work days, BPD adolescents spent more time awake when they were in bed compared to HC and BD adolescents (p = 0.039). On schedule-free days, BPD and BD youths spent more time in bed compared to HC adolescents (p = 0.015). BPD adolescents woke up over 1 h later compared to HC (p = 0.003). Total sleep time was more variable between nights in BPD adolescents compared to the HC group (p = 0.031). Future research should explore if sleep-wake pattern disruptions are a cause or a consequence of BPD symptomatology in adolescents. Addressing sleep-wake pattern during clinical assessment and treatment of BPD adolescents may potentially reduce their symptoms; this therapeutic effect still needs to be evaluated
Quantitative, Competitive PCR and Fluorescent Microscopy Methods for the Study of Batrachochytrium dendrobatidis
No full textChytridiomycosis is a disease affecting amphibian populations worldwide and is caused by the parasitic fungus Batrachochytrium dendrobatidis (Bd). The catastrophic decline in numbers of amphibians dictates the need for full characterization of the fungus and amphibian host responses. Significant areas of research that would benefit from strengthening available methods are diagnostic techniques and fundamental molecular biology. A quantitative, competitive PCR (QC-PCR) technique is described that will improve cost efficiency of collecting data on the quantification of Bd in infected animals and the environment. QC-PCR adapts conventional PCR reagents and a competitor DNA sequence into a quantitative technique. Fluorescent microscopy is a vital tool used to study cellular and molecular biology. DNA and cell wall fluorescent staining of viable Bd observed with laser scanning confocal microscopy is described and should provide the foundation for future studies of Bd involving fluorescent microscopy. Both QC-PCR and fluorescent microscopy are technique advancements that will contribute to future studies and to understanding of Bd and chytridiomycosis
Cytochrome bd displays significant quinol peroxidase activity
No full textCytochrome bd is a prokaryotic terminal oxidase that catalyses the electrogenic reduction of oxygen to water using ubiquinol as electron donor. Cytochrome bd is a tri-haem integral membrane enzyme carrying a low-spin haem
Actigraphic assessment of delayed sleep–wake rhythm could predict functional impairment in patients with bipolar disorder
No full textAbstract Aim A delayed sleep–wake rhythm is a common but often under‐recognized characteristic of bipolar disorder (BD). This study aimed to examine the association between delayed sleep–wake rhythm and functional impairment using actigraphy. Methods Participants with BD in clinical remission (N = 47) were recruited. Sleep parameters, including the midpoint of sleep (o'clock), total sleep time (minutes), sleep efficiency (%), and wake after sleep onset (minutes), were averaged over a 14‐day period using continuous actigraphy. Functional impairment was assessed using the 12‐item World Health Organization Disability Assessment Schedule 2.0 (WHODAS 2.0). Correlation analyses were conducted to examine associations between total WHODAS 2.0 scores and sleep parameters. Multiple regression analyses were performed with WHODAS 2.0 total scores as the dependent variable and sleep parameters as independent variables, controlling for mood symptoms and insomnia severity. Results Correlation analysis revealed a significant positive relationship between the midpoint of sleep and total WHODAS 2.0 scores (r = 0.424, p = 0.003). Multiple regression analysis identified the midpoint of sleep as a significant predictor of total WHODAS 2.0 scores (β = 0.488, p = 0.005). Conclusion Delayed sleep–wake rhythms may contribute to functional impairment in euthymic individuals with BD. These findings suggest that advancing sleep–wake rhythm may contribute to improvements in social functioning. Actigraphy holds potential as a digital biomarker for assessing functional outcomes in this population
BD Rhapsody WTA AbSeq Analysis
No full text<p>Dataset and codes for BD Rhapsody single cell analysis system.</p>
<p>Disclaimer: The set of codes are optimised for the associated dataset only. For detailed understanding of the analysis procesure, please refer to https://github.com/INGEN-HOPE/Single-Cell-Multi-Omics-Data-Analysis-for-BD-Rhapsody. For any kind queries, please refer to the corresponding author, or post your query on our GitHub page.</p>
Sleep-wake cycle phenotypes in young people with familial and non-familial mood disorders
No full textObjectives: Converging evidence identifies that the offspring of parents with bipolar disorder (BD), individuals at clinical high risk of BD, and young people with recent onset BD may differ from other clinical cases or healthy controls in terms of sleep-wake profiles. However, it is possible that these differences may reflect current mental state, subtype of mood disorder, or familial traits. This study aimed to determine objective and subjective sleep-wake profiles in individuals aged 15-25 years with a current major depressive episode, in relation to familial traits.Methods: Frequency matching was employed to ensure that each individual with a confirmed family history of BD (FH+) could be compared to four controls who did not have a familial mood disorder (FH-). Pre-selected objective actigraphy and subjective Pittsburgh Sleep Quality Index (PSQI) ratings were compared using one-way analysis of variance (ANOVA) and applying the Benjamini-Hochberg (BH) correction for false discoveries.Results: The sample comprised 60 individuals with a mean age of 19 years. The FH+ (n=12) and FH-groups (n=48) differed on three key sleep parameters: mean sleep duration on week nights (P=.049), variability in waking after sleep onset (P=.038), and daily disturbances (PSQI dimension of sleep disturbance and daytime dysfunction; P=.01).Conclusions: The sleep profiles we identified in this study, especially the daily disturbances phenotype, provide support for research into endophenotypes for BD. Also, the findings may offer the opportunity for more tailored, personalized interventions that target specific components of the sleep-wake cycle in individuals with a family history of BD
Sleep-wake cycle phenotypes in young people with familial and non-familial mood disorders
No full textObjectives: Converging evidence identifies that the offspring of parents with bipolar disorder (BD), individuals at clinical high risk of BD, and young people with recent onset BD may differ from other clinical cases or healthy controls in terms of sleep–wake profiles. However, it is possible that these differences may reflect current mental state, subtype of mood disorder, or familial traits. This study aimed to determine objective and subjective sleep–wake profiles in individuals aged 15–25 years with a current major depressive episode, in relation to familial traits. Methods:Frequency matching was employed to ensure that each individual with a confirmed family history of BD (FH+) could be compared to four controls who did not have a familial mood disorder (FH–). Pre-selected objective actigraphy and subjective Pittsburgh Sleep Quality Index (PSQI) ratings were compared using one-way analysis of variance (ANOVA) and applying the Benjamini–Hochberg (BH) correction for false discoveries. Results: The sample comprised 60 individuals with a mean age of 19 years. The FH+ (n=12) and FH– groups (n=48) differed on three key sleep parameters: mean sleep duration on week nights (P=.049), variability in waking after sleep onset (P=.038), and daily disturbances (PSQI dimension of sleep disturbance and daytime dysfunction; P=.01). Conclusions: The sleep profiles we identified in this study, especially the daily disturbances phenotype, provide support for research into endophenotypes for BD. Also, the findings may offer the opportunity for more tailored, personalized interventions that target specific components of the sleep–wake cycle in individuals with a family history of BD.8 page(s
- …
