30,200 research outputs found
The adjuvant MF59 induces ATP release from muscle that potentiates response to vaccination
No full textVaccines are the most effective agents to control infections [1]. In addition to the pathogen antigens, vaccines contain adjuvants that are used to enhance the specific immune responses. Despite their effectiveness and their wide use, the mechanism of action of many adjuvants is poorly characterized [2]. Therefore, adjuvant research is crucial to better understand how they work and to exploit their full potential in vaccinology [3]. Release of endogenous danger signals has been linked to adjuvanticity, however the role of extracellular ATP during vaccination has never been explored. Extracellular ATP can work as "danger signal" and, as such is a strong modulator of immune responses [4-6]. Here, we tested whether ATP release is involved in the immune boosting effect of four common adjuvants: aluminium hydroxide, calcium phosphate (CaPi), incomplete Freund’s adjuvant (IFA) and the squalene-based oil in water emulsion MF59.
Experiments were performed ex vivo in excised mice muscles (tibialis anterior and quadriceps) and in vivo in live mice injected with the reporter system luciferase-luciferin that reports on ATP changes. We found that intramuscular injection in general is always associated to a weak transient release of ATP. In contrast, a greatly enhanced ATP release was found upon injection of MF59 but not by all other adjuvants tested.
Therefore, we wanted to dissect whether and how ATP release would contribute to the activity of MF59. The strong adjuvanticity of MF59 [7-8] has been ascribed to its capability to induce an immunocompetent environment in the muscle, characterized by a rapid and transient influx of a large number of immune cells participating in antigen uptake and transport to draining lymph nodes [9-11]. We found that the local injection of apyrase, an ATP-hydrolyzing enzyme, reduced the immune cells recruitment induced by MF59 but not by alum or IFA. These findings indicated that the ability of MF59 to induce migration of different immune cells into the injected muscle is partly due to induced ATP release. Moreover, co-injection of apyrase and MF59 at the muscle injection site reduces the number of antigen positive cells in the draining lymph nodes in a cell type-specific manner. Indeed, co-injection of apyrase negatively impacts the number of antigen positive B cells induced by MF59, suggesting that B cells could be a key component in ATP-mediated signaling during vaccination. Strong innate immune responses lead to enhanced adaptive immune responses [12]. Accordingly, we compared the impact of MF59-induced ATP release on T cells responses and antibody titers. Groups of mice were immunized with an experimental trivalent influenza vaccine (TIV) either as plain antigens or together with MF59 with or without apyrase. Apyrase strongly inhibited influenza specific T cell responses, total IgG and hemagglutination inhibition titers in response to an MF59-adjuvanted trivalent influenza vaccine. These data demonstrate that a transient ATP release is required for innate and adaptive immune responses induced by MF59 and link for the first time extracellular ATP to an enhanced response to vaccination
TRATAMENTO ENDODÔNTICO EM SESSÃO ÚNICA DO MOLAR SUPERIOR COM QUATRO CANAIS RADICULARES REALIZADO COM LIMA VONO.
No full textThis is a case report that aims to describe the endodontic treatment of the upper right first molar with mesiovestibular canal 2 (MV2). A 54-year-old male patient attended a private practice and, after anamnesis and clinical examination, was diagnosed with irreversible pulpitis. Treatment was performed in a single session, starting with anesthesia, absolute isolation and access surgery. After locating all canals with the The Finder ultrasound insert (Helse, Brazil), chemical-mechanical preparation was performed with the Vono file system: enlargement of the cervical and middle thirds with Vono rotary file # 15.08, and upon reaching the apparent length of the tooth, foraminal electronic odontometry was performed. Preparation was then conducted with Vono files numbers # 15.03, # 20.04 and # 25.04 and 2.5% sodium hypochlorite irrigating solution. The obturation technique of choice was a single cone, with Sealer Plus resin cement (MK Life, Brazil). Coronal shielding was performed at the end of the session. It was concluded that the Vono files provided excellent modeling of the root canals, facilitating the obturation of the root canal system, including the MV2.Se presenta un reporte de caso con el objetivo de describir el tratamiento endodóntico del primer molar superior derecho con canal mesiovestibular 2 (MV2). : Paciente masculino de 54 años que acudió a consultorio privado y tras anamnesis y exploración clínica se le diagnosticó pulpitis irreversible. El tratamiento se realizó en una sola sesión, iniciando con anestesia, aislamiento absoluto y cirugía de acceso, luego de localizar todos los canales con el inserto ultrasónico The finder (Helse, Brasil), se realizó la preparación químico-mecánica con el sistema de limas Vono: aumento de tercio cervical y medio con lima rotatoria Vono #15.08, la cual al alcanzar la longitud aparente del diente se realizó odontometría foraminal electrónica. Luego se realizó la preparación con limas Vono #15.03, #20.04 y #25.04 y solución irrigante de hipoclorito de sodio al 2,5%. La técnica de obturación de elección fue de cono único, con cemento resinoso Sealer Plus (MK Life, Brasil), al finalizar la sesión se realizó blindaje coronario. Se concluye que las limas Vono proporcionaron un excelente modelado de los conductos radiculares, facilitando la obturación del sistema de conductos radiculares, incluido MV2.Trata-se de um relato de caso com objetivo de descrever o tratamento endodôntico do primeiro molar superior direito com canal mésio-vestibular 2 (MV2). Paciente do gênero masculino, 54 anos de idade, compareceu a consultório particular e após anamnese e exame clínico recebeu o diagnóstico de pulpite irreversível. O tratamento foi realizado em sessão única, iniciando pela anestesia, isolamento absoluto e cirurgia de acesso, após localização de todos os canais com o inserto de ultrassom The finder (Helse, Brasil), o preparo químico-mecânico foi realizado com o sistema de limas Vono: ampliação dos terços cervical e médio com lima rotatória Vono # 15.08, que ao chegar no comprimento aparente do dente, realizou-se a odontometria eletrônica foraminal. O preparo então foi conduzido com as limas Vono de números #15.03, #20.04 e #25.04 e solução irrigadora hipoclorito de sódio a 2,5%. A técnica de obturação de escolha foi de cone único, com cimento resinoso Sealer Plus (MK Life, Brasil), finalizando a sessão foi realizada a blindagem coronária. Conclui-se que as limas Vono determinaram uma excelente modelagem dos canais radiculares, facilitando a obturação do sistema de canais radiculares, incluindo o MV2
Maria Bersani
No full textLa voce illustra la biografia e l'apporto letterario dato da Maria Bersani alla letteratura per l'infanziaThe headword explains the biography and the contribution of the author Maria Bersani to the children's literatur
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
O curso de licenciatura em educação física da Universidade Federal de Santa Catarina: suas concepções de ensino e de educação física
No full textDissertação (mestrado) - Universidade Federal de Santa Maria. Centro de Educação Fisica e Desporto
Erratum: Lack of immunity against rubella among Italian young adults. [BMC Infect Dis., 17, (2017) (199)] Doi: 10.1186/s12879-017-2295-y
No full textAfter publication of this article [1], the authors noted that the given names and family names of all authors had been inverted, and are therefore incorrect in the original article. In the original article, the author names appear as the following: Gallone Maria Serena, Gallone Maria Filomena, Larocca Angela Maria Vittoria, Germinario Cinzia and Tafuri Silvio. However, this is incorrect, and the author names should appear as per the below: Maria Serena Gallone, Maria Filomena Gallone, Angela Maria Vittoria Larocca, Cinzia Germinario, Silvio Tafuri. The author names have been corrected in the author list and the citation for this Erratum
Imagens de Otto Maria Carpeaux: esboço de biografia
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em História, Florianópolis, 2015.Este esboço de biografia procura citar algumas imagens de Otto Maria Carpeaux: construções biográficas de naturezas múltiplas, elaboradas em contextos, por atores e sob condições igualmente díspares. Está constituído a partir de uma visão crítica da História, o que permite que ?outras imagens?, fragmentárias e não monumentais, também tenham espaço. Em diálogo com o princípio da montagem, este esboço apresenta-se em duas partes. Na primeira, Imagens possíveis, estão citadas as imagens elaboradas em vida e post mortem acerca do austríaco-brasileiro que nasceu em Viena em 1900, se exilou no Brasil em 1939 e morreu no Rio de Janeiro, em 1978. Na segunda, Montagens possíveis, apresentam-se duas possibilidades de exercício biográfico: pela leitura alegórica do documentário O velho e o Novo (Otto Maria Carpeaux), entendido como instrumento de intervenção no contexto ditatorial brasileiro e de uma reelaboração biográfica concernentes às suas experiências europeias; e pelo Caderno de imagens críticas, registro dos encontros em Carpeaux pelo meio de imagens críticas produzidas a partir da cesura do presente.Abstract : This biographical sketch attempts to quote some images of Otto Maria Carpeaux: various types of biographical constructions, carried out in different contexts by disparate authors under conditions just as distinct. It stems from a critical view of history, allowing for ?other images? fragmented and non-monumental ? to share the space.In dialogue with the montage principle, this sketch has two parts. The first, Possible Images, quotes the images produced during and after the life of the Austrian-Brazilian, who was born in Vienna in 1900, went to Brazil in exile in 1939 and died in Rio de Janeiro in 1978. The second part, Possible Montages, presents two possibilities of a biographical exercise: through the allegorical reading of documentary O Velho e o Novo (Otto Maria Carpeaux), understood as an instrument of intervention in the Brazilian dictatorship context and as a biographical retelling of the author?s European experiences; and through my Scrapbook of Critical Images, a record of the encounters in Carpeaux through critical images produced from the caesura of the present
Maria José ao encontro de Tchekov
No full text“A Carta da Corcunda para o Serralheiro” (“Letter from the Hunchback Girl to the Metalworker”), signed by Maria José, was first performed in a theatre show in 1988 (by actress-director Maria do Céu Guerra), two years before the typescript was published in book form for the first time by Teresa Rita Lopes. The evidence upon which this article is built is that it was the theatre that revealed the dramatic destination of this singular epistle of the only female heteronym that Fernando Pessoa invented, which was also the last heteronym to be created by the author. The dramatic construction of Maria José can be approximated to the character-building strategies in Anton Chekhov’s realist dramaturgy, in a way that differs from Pessoa’s other dramaturgical experiences. The analysis of Chekhov’s ascendancy over Stanislavski’s research, as well as the importance of Schopenhauer’s conception of the tragicomic, which is simultaneously projected onto Chekhov’s theatre and Maria José’s profile, are favourable lines of reading for this rapprochement between Pessoa and the Russian fiction writer and playwright. Pessoa’s readings of Chekhov’s texts (such as the play Uncle Vania and the short story “Vanka”) provide relevant data for assessing the magnitude of this encounter.“A Carta da Corcunda para o Serralheiro”, assinada por Maria José, começou por ser difundida num espectáculo de teatro em 1988 (pela actriz-encenadora Maria do Céu Guerra) dois anos antes de o dactiloscrito se ver publicado em livro, pela primeira vez, por Teresa Rita Lopes. Parte-se da evidência de ter sido o teatro a revelar a destinação dramática da epístola singular do único heterónimo mulher que Fernando Pessoa inventou, que terá sido também o último a ser criado pelo autor. A construção dramática de Maria José pode ser aproximada às estratégias de construção de personagem na dramaturgia realista de Anton Tchekov, de um modo que se distingue de outras experiências dramatúrgicas de Pessoa. A análise do ascendente de Tchekov sobre as pesquisas de Stanislavski, bem como a importância da concepção de tragicómico em Schopenhauer, que se projecta em simultâneo sobre o teatro de Tchekov e sobre o perfil de Maria José, são linhas de leitura propícias para esta aproximação entre Pessoa e o ficcionista e dramaturgo russo. As leituras de textos de Tchekov por parte de Pessoa (como o caso da peça Tio Vânia e o conto “Vanka”) fornecem dados relevantes para avaliar a magnitude deste encontro
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