593 research outputs found
Current and historic gene flow of the sand goby <i>Pomatoschistus minutus</i> on the European Continental Shelf and in the Mediterranean Sea
No full textPhylogeographical patterns of the sand goby Pomatoschistus minutus (Gobiidae, Teleostei) were studied by means of sequence and single-stranded conformational polymorphism analysis of a 283-bp fragment of the cytochrome b locus of the mtDNA. A total of 228 individuals sampled at 13 sites throughout the species's distributional range revealed a moderate level of diversity and a low but significant level of overall genetic differentiation at all but one site. The goby sample from the Adriatic Sea differed in sequence by approximately 10% from the Atlantic P. minutus and is thought to belong to a cryptic species of the genus Pomatoschistus. Limited genetic differentiation with a weak pattern of isolation-by-distance was recorded throughout the distributional range of the typical P. minutus. Phylogeographical analysis suggested a contiguous range expansion in the Atlantic and Baltic basins during the Eemian and evidence for a glacial refugium in the southern North Sea during the Weichselian. In P. minutus from the western Mediterranean Sea a high number of endemic haplotypes as well as the most common Atlantic haplotype were recorded in appreciable frequencies. This might be explained by secondary contact between different mitochondrial lineages, which evolved in allopatry
Archives affabulatoires, engagisme queer et ZOM-FAM de Kama La Mackerel
No full textL’auteure avance que le recueil de poésie ZOM-FAM, de Kama La Mackerel, constitue une archive affabulatoire de l’engagisme queer (queer indenture). Elle situe d’abord son recueil dans le contexte des histoires d’esclavage et d’engagisme de l’île Maurice, pour observer ensuite la façon dont ZOM-FAM offre une optique queer par des approches décoloniales au langage, ainsi qu’une esthétique qui éclaire de nouvelles généalogies d’engagisme queer et de genre décolonial.The author proposes that Kama La Mackerel’s ZOM-FAM is a fabulatory archive for queer indenture. She contextualizes this book of poems within Mauritius and the island’s histories of enslavement and indenture and observe how ZOM-FAM offers a queers lens through decolonial approaches to language alongside an aesthetic that illuminates new genealogies in queer indenture and decolonial gender.La autora afirma que la colección de poesía ZOM-FAM, de Kama La Mackerel, constituye un archivo ficticio de engagisme queer (queer indenture). En primer lugar, sitúa su colección en el contexto de los relatos de esclavitud y de engagisme de la Isla Mauricio, luego observa cómo ZOM-FAM ofrece una perspectiva queer a través de enfoques decoloniales del lenguaje, así como una estética que alumbra nuevas genealogías de engagisme queer y de género decolonial
Magnetospirillum sulfuroxidans sp. nov., capable of sulfur-dependent lithoautotrophy and a taxonomic reevaluation of the order Rhodospirillales
No full textA spiral-shaped, highly motile bacterium was isolated from freshwater sulfidic sediment. Strain J10 T is a facultative autotroph utilizing sulfide, thiosulfate, and sulfur as the electron donors in microoxic conditions. Despite high 16S rRNA gene sequence sequence identity to Magnetospirillum gryphiswaldense MSR-1 T (99.6 %), digital DNA-DNA hybridisation homology and average nucleotide identity between the two strains was of the different species level (25 % and 83 %, respectively). Strain J10 T is not magnetotactic. The DNA G + C content of strain J10 T is 61.9 %. The predominant phospholipid ester-linked fatty acids are C18:1ω7, C16:1ω7, and C16:0. Strain J10 T (=DSM 23205 T = VKM B-3486 T) is the first strain of the genus Magnetospirillum showing lithoautotrophic growth and is proposed here as a novel species, Magnetospirillum sulfuroxidans sp. nov. In addition, we propose to establish a framework for distinguishing genera and families within the order Rhodospirillales based on phylogenomic analysis using the threshold values for average amino acid identity at ̴ 72 % for genera and ̴ 60 % for families. According to this, we propose to divide the existing genus Magnetospirillum into three genera: Magnetospirillum, Paramagnetospirillum, and Phaeospirillum, constituting a separate family Magnetospirillaceae fam. nov. in the order Rhodospirillales. Furthermore, phylogenomic data suggest that this order should accomodate six more new family level groups including Magnetospiraceae fam. nov., Magnetovibrionaceae fam. nov., Dongiaceae fam. nov., Niveispirillaceae fam. nov., Fodinicurvataceae fam. nov., and Oceanibaculaceae fam. nov. Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.BT/Environmental Biotechnolog
Dethiobacteraceae fam. nov.
No full textDe.thi’o.bac.ter.a.ce.a.e. N.L. masc. n. Dethiobacter, the type genus of the family; L. fem. pl. n. suff. -aceae, ending to denote a family; N.L. fem. pl. n. Dethiobacteraceae, the family of the genus Dethiobacter. The family Dethiobacteraceae includes obligately anaerobic, moderately salt-tolerant, and obligately alkaliphilic bacteria able to grow chemolithoautotrophically by elemental sulfur disproportionation and fixingCO2 by the Wood–Ljungdahl pathway. The two strains currently known in pure culture are both isolated from saline soda lakes. H2 can serve as an additionalelectron donor for sulfur and thiosulfate reduction. The family consists of a single genus Dethiobacter with the type species Dethiobacter alkaliphilus. DNA G +C content (%): 48.3–48.5 (genomes of two isolates). Type genus: Dethiobacter Sorokin et al. 2008, VL123.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work publicBT/Environmental Biotechnolog
Phylogenetic study of the Nemaliales (Rhodophyta) based on large-subunit ribosomal DNA sequences supports segregation of the Scinaiaceae fam. nov. and resurrection of Dichotomaria Lamarck
No full textGene sequence data have been newly obtained for 18 species in 13 genera of the order Nemaliales (Rhodophyta), allowing for the first time a relatively comprehensive molecular phylogenetic appraisal of the order. The phylogenetic trees generated from these data support the recognition of three families: (i) the Liagoraceae as presently constituted; (ii) a reduced Galaxauraceae includingActinotrichia,Galaxaura (sensu lato), andTricleocarpa; and (iii) a new family, Scinaiaceae, segregated from the Galaxauraceae and including the generaScinaia,Gloiophloea,Nothogenia, and probablyWhidbeyella. The four genera of the Scinaiaceae differ from the newly circumscribed Galaxauraceae in being uncalcified, and having heteromorphic life histories in which the tetrasporophyte is much reduced and filamentous or crustose. This type of life history is found in onlyTricleocarpaof the Galaxauraceae. The results also showGalaxaurato be para/polyphyletic ifActinotrichiaandTricleocarpaare recognized. To remedy this, theGalaxaura marginataspecies complex,Galaxaura diesingiana, andGalaxaura obtusataare removed from the genus and placed in the resurrectedDichotomariaLamarck.Galaxaura marginata, presently thought to be wide-ranging and morphologically variable, is shown to comprise several species. As a consequence,Galaxaura teneraKjellman andBrachycladia australisSonder are removed from the synonymy ofG. marginataand restored as independent species inDichotomariafor South African and Australian isolates, respectively. The Liagoraceae is shown to encompass genera previously placed in the segregate families Nemaliaceae and Dermonemataceae, and the value of the reproductive characters used to define those taxa is discussed. [ABSTRACT FROM AUTHOR]Peer reviewedfinal article publishedDichotomariaScinaiaceae fam. novphylogenyNemalialesLiagoraceaeGalaxaura marginataGalaxauracea
Comparative visual and DNA-based diet assessment extends the prey spectrum of polar cod Boreogadus saida
No full textsponsorship: Research was funded by Research Foundation Flanders (FWO) through a fellowship for strategic basic research (FWO-SB, grant no. 1S04418N) and by a Brilliant Marine Research Idea Philanthropy Award 2019 granted by the Flanders Marine Institute (VLIZ), Belgium, to S.M.M. and was carried out with infrastructure support from European Marine Biological Resource Centre (EMBRC) Belgium-FWO project I001621N. H.C. was supported by a fellowship from the former Flemish Agency for Innovation by Science and Technology, now Flanders Innovation & Entrepreneurship (VLAIO, grant no. 141328). Fieldwork was supported by the Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research (AWI), under expedition grant no. AWI_PS106/1_2-00 and the Netherlands Ministry of Agriculture, Nature and Food Quality (LNV) under its Statutory Research Task Nature & Environment WOT-04-009-047.04. We thank Captain Thomas Wunderlich and the crew of RV 'Polarstern' expedition PS106/2 for their support. We are very grateful to everyone who collected samples, that is Nils Koschnick, Jennifer Steffen, Erik Sulanke, Kim Vane and Susanne Fuchs with the assistance of Elisa Bravo Rebolledo, Giulia Castellani and Julia Ehrlich. We sincerely thank Birte Matthiessen, Paul E. Renaud and 2 anonymous reviewers for their constructive comments that helped improve the paper. Polar cod were sampled and processed according to and within the laws, guidelines and policies of the German Animal Welfare Organisation; no specific permissions were required. The fish collected are neither endangered nor protected in the coastal waters of the Svalbard Archipelago. Polar cod were sacrificed immediately after sampling. (Research Foundation Flanders (FWO) through a fellowship for strategic basic research (FWO-SB)|1S04418N, Flanders Marine Institute (VLIZ), Belgium, European Marine Biological Resource Centre (EMBRC) Belgium-FWO|I001621N, Flanders Innovation & Entrepreneurship (VLAIO)|141328, Helmholtz Centre for Polar and Marine Research (AWI)|AWI_PS106/1_2-00, Netherlands Ministry of Agriculture, Nature and Food Quality (LNV) under its Statutory Research Task Nature Environment|WOT-04-009-047.04, Captain Thomas Wunderlich, Alfred Wegener Institute, Academy of Finland (AKA)|141328)status: Published onlin
Reconstructing colonization dynamics of the human parasite Schistosoma mansoni following anthropogenic environmental changes in northwest Senegal
No full textSchistosoma parasites successfully colonize new regions following the construction of water schemes for power production or agricultural purposes. Here we investigated the colonization history of the human parasite Schistosoma mansoni in Northwest Senegal following the construction of two dams in the `80s. Parasites were collected at several time points following the disease outbreak and their genetic profile was characterized using molecular markers. Our results showed that many genetically different parasites must have been introduced at the onset of the epidemic, which complies with the extensive immigration of infected seasonal agricultural workers from neighboring regions in Senegal, Mauritania and Mali. Furthermore, we showed that parasite transmission occurred over a large geographic distance, which implies that new alleles, like resistance alleles, could spread rapidly in this system. These new insights demonstrate how colonization following anthropogenic environmental changes may lead to genetically diverse parasite populations within a short time span. High genetic diversity is often linked with a stronger potential to cope with selective pressures such as drug treatment, which may complicate efforts to control the disease
Sulfurivermis fontis gen. nov., sp nov., a sulfur-oxidizing autotroph, and proposal of Thioprofundaceae fam. nov
No full textA novel Gram-stain-negative, chemolithoautotrophic sulfur oxidizer, strain JG42(T), was isolated from a hot spring microbial mat. As an electron donor for autotrophic growth, strain JG42(T) utilized sulfide, thiosulfate, tetrathionate and elemental sulfur. Cells of strain JG42(T) were oxidase-positive and catalase-negative. The G+C content of the genomic DNA was 65 mol%. The predominant cellular fatty acid was C-16:0. Phylogenetic analysis of the 16S rRNA gene indicated that strain JG42(T) belonged to the order Chromatiales, but sequence similarities to the known species were less than 94 %. On the basis of its properties, strain JG42(T) (= DSM 104776(T) = NBRC 112696(T)) is proposed as the type strain of a novel species of a new genus, Sulfurivermis fontis gen. nov., sp. nov., which belongs to the family Thioalkalispiraceae. A new family, Thioprofundaceae fam. nov., is also proposed to accommodate the genus Thioprofundum, transferred from the family Thioalkalispiraceae
Natronogracilivirga saccharolytica gen. nov., sp. nov. and Cyclonatronum proteinivorum gen. nov., sp. nov., haloalkaliphilic organotrophic bacteroidetes from hypersaline soda lakes forming a new family Cyclonatronaceae fam. nov. in the order Balneolales
No full textTwo heterotrophic bacteroidetes strains were isolated as satellites from autotrophic enrichments inoculated with samples from hypersaline soda lakes in southwestern Siberia. Strain Z-1702 T is an obligate anaerobic fermentative saccharolytic bacterium from an iron-reducing enrichment culture, while Ca. Cyclonatronum proteinivorum Omega T is an obligate aerobic proteolytic microorganism from a cyanobacterial enrichment. Cells of isolated bacteria are characterized by highly variable morphology. Both strains are chloride-independent moderate salt-tolerant obligate alkaliphiles and mesophiles. Strain Z-1702 T ferments glucose, maltose, fructose, mannose, sorbose, galactose, cellobiose, N-acetyl-glucosamine and alpha-glucans, including starch, glycogen, dextrin, and pullulan. Strain Omega T is strictly proteolytic utilizing a range of proteins and peptones. The main polar lipid fatty acid in both strains is iso-C 15:0, while other major components are various C 16 and C 17 isomers. According to pairwise sequence alignments using BLAST Gracilimonas was the nearest cultured relative to both strains (<90% of 16S rRNA gene sequence identity). Phylogenetic analysis placed strain Z-1702 T and strain Omega T as two different genera in a deep-branching clade of the new family level within the order Balneolales with genus. Based on physiological characteristics and phylogenetic position of strain Z-1702 T it was proposed to represent a novel genus and species Natronogracilivirga saccharolityca gen. nov., sp. nov. (= DSMZ 109061 T =JCM 32930 T =VKM B 3262 T). Furthermore, phylogenetic and phenotypic parameters of N. saccharolityca and C. proteinivorum gen. nov., sp. nov., strain Omega T (=JCM 31662 T, =UNIQEM U979 T), make it possible to include them into a new family with a proposed designation Cyclonatronaceae fam. nov. Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.BT/Environmental Biotechnolog
The Relation between Treated Maternal Urinary Tract Infection and Adverse Maternal, Prenatal Outcomes in Pregnant Women of Ardabil, Iran
No full textBackground and Objective: Urinary tract infection is one of the most common bacterial infections during pregnancy and has also been implicated as a risk factor for adverse maternal and prenatal outcomes. The aim of our study was to determine the relation between maternal urinary tract infection and adverse maternal, prenatal outcomes in pregnant women of Ardabil, Iran.
Material and Methods: This retrospective-case-control study was conducted on prenatal file records of pregnant women in Ardabil (2011). The pregnant women who had a positive urine culture in their prenatal files (N= 211) were considered as a case group and 232 ones without urinary tract infection as a control. Using a research- made questionnaire, the data related to present pregnancy and prenatal information was collected and analyzed by KrusKal Wallis, Chi- Square and Fisher statistical tests.
Results: Maternal age of under 25 (%61.6 vs. 56.5), body mass index of more than 30 (%18.3 vs. 15.6), primigravida (%55 vs. 48.8), hypertension (%2.4 vs. 1.3), hyperemesis Gravidarum (%14.8 vs. 12.6), frequency and dysuria (%1.9 vs. 0.9), low birth weight (%95.4 vs. 93.2), congenital malformation (%3.5 vs. 1.8), artificial milk feeding (%6.5 vs. 2.7), neonatal death (%0.9 vs. 0.0) are higher in urinary infection group, however the differences are not statistically significant. Other maternal and prenatal adverse outcomes such as diabetes, pre-eclampsia , hemoglobin level, prematurity, abortion and stillbirth have not significant relation with urinary infection.
Conclusion: Because of low level of adverse maternal or prenatal outcomes reported in our study, we conclude that screening and treatment of urinary tract infection in Ardabil health service is appropriate; therefore, no change is needed for present screening or treatment processes
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