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    Hidrogéis de celulose bacteriana incorporados com frações de Aloe vera

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    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2014.A Aloe vera, popularmente denominada babosa no Brasil, é uma planta originária da África, utilizada por culturas antigas do Mediterrâneo e do Egito devido às suas propriedades terapêuticas e medicinais. O extrato do parênquima de reserva desta planta, denominado gel de A. vera, apresenta ampla gama de compostos que possuem atividades farmacológicas de interesse medicinal. A incorporação de porções de A. vera no desenvolvimento de novos materiais, como a celulose bacteriana (CB), promove alterações morfológicas, mecânicas e químicas de grande interesse para engenharia tecidual e na produção de novas classes de dispositivos biomédicos. Na produção dos hidrogéis celulose bacteriana e A. vera, o meio de cultura da bactéria Gluconacetobacter hansenii foi formulado com três porções de A. vera, variando as concentrações de 20%, 40%, 60%, 80% a 100% (v/v). Apenas as formulações de 20%, 40% e 60% (v/v), das três porções, produziram com sucesso materiais com características distintas e singulares de microestrutura, caracterizadas por microscopia eletrônica de varredura (MEV); de propriedades mecânicas, químicas, avaliadas qualitativamente por espectroscopia de infravermelho por refletância total atenuada (FTIR-ATR); e também foram caracterizadas quanto à capacidade de absorção de água e cristalinidade. Além disso, células de fibroblastos da linhagem L929, foram semeadas sobre os materiais para avaliação da citotoxicidade, adesão e morfologia celular em função do tempo. Os fibroblastos permaneceram viáveis em até 48 horas de cultura em todos os hidrogéis desenvolvidos e apresentaram morfologia alongada e melhor aderência nas membranas formuladas com 60% das três porções. Os materiais formulados com 60% de A. vera na celulose bacteriana se mostraram plataformas promissoras para o desenvolvimento de dispositivos para regeneração de pele.Abstract : Aloe vera, usually known in Brazil as "babosa", originary plant from Africa, was used by ancient cultures of the Mediterranean and Egypt due to therapeutic and medicinal properties. The parenchyma reserve extract of this plant, called A. vera gel, presents a wide range of compounds which has pharmacological activities of medicinal interests. The incorporation of A. vera portions in development of new materials, such as bacterial cellulose (BC), promotes morphological, chemical and mechanical changes of great interests for tissue engineering and production of new classes of biomedical devices. In the production of bacteria cellulose and A. vera hydrogels, the culture medium of the bacteria Gluconacetobacter hansenii was formulated with three portions A. vera, raging the concentrations from 20%, 40 %, 60 %, 80% to 100% (v/v). Only formulations of 20 % , 40 % and 60 % (v/v), for three portions, produced successfully materials with distinct and unique characteristics of microstructure; characterized by scanning electron microscopy (SEM); mechanical and chemical properties; evaluated qualitatively by infrared spectroscopy by attenuated total reflectance (FTIR-ATR), and were also characterized by their ability to absorb water and crystallinity. Moreover, fibroblast cells of the L929 strain were seeded on the material to evaluate cytotoxicity, cell adhesion and morphology as a function of time. Fibroblasts remained viable for up to 48 hours of culture, in all developed hydrogels, and showed elongated morphology and better adhesion on the membranes formulated with 60 % of the three portions. The materials formulated with 60% of A. vera into bacterial cellulose proved are promising scaffold for the development of new devices for skin regeneration

    Dr Vera Scantlebury and her brother Dr George Clifford Scantlebury

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    This record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/336700Photograph of Lieutenant (Dr) Vera Scantlebury (1889-1946) and her brother Captain (Dr) George Clifford Scantlebury (1890-1976) dressed in WWI military uniforms. Commencing 1917 Vera was enlisted in the Royal Army Medical Corps as assistant surgeon at Endell Street Military Hospital, England.135952 Item: [2013.0058.00001] "Dr Vera Scantlebury and her brother Dr George Clifford Scantlebury

    Project of the Fashion Industries Oral History Series: Vera Maxwell Part 2

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    Vera Maxwell was New York City in 1901. She trained as a ballet dancer and joined the Metropolitan Opera Ballet in 1919. In 1924, she married Raymond J. Maxwell and shortly after began her first foray into the fashion world as a model for a wholesale company, eventually starting to sketch, design and model her own clothes in 1929. By 1936 Vera's designs were receiving positive reviews in the fashion press and her freelance collections were receiving attention in New York. She was also designing for 7th Avenue firms such as Adler and Adler, Glenhunt and Max Milstein. Vera opened Vera Maxwell Originals in 1946 and became known for her classic separates and suits, dresses teamed with jackets, print dresses, Chesterfield coats and Wraparound jersey dresses. During the war years, she gained a reputation for her practical sense, which was evident in the one-piece overalls she designed for female factory workers. Her simple lapel-less suits were spare and gave no hint of having been stripped of their decorations, which demonstrated her skill at overcoming the fabric rations of the time. Other innovations Vera became known for were her desire to design for women of short height, or without a model's figure and for women whose jobs took them beyond their desks. In 1974, she designed a "speedsuit" made of jersey with an elasticized waist, which could be pulled on in just 17 seconds. Vera closed her business in 1985, eventually passing away in January 1995."Vera Maxwell was a designer of American Classics. She started in the coat business. Nancy White, then working at Home and Garden, asked her to do children's coats for the magazine, which she did. She later got into women's fashion. “Vera’s a designer who understands women and understands their needs. There is a timelessness about her clothes, as we know, and thank heaven there is.”" - Kathy TankusThird interview in a series of three of Vera Maxwell, possibly conducted by John Touhey

    Project of the Fashion Industries Oral History Series: Vera Maxwell Part 3

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    Vera Maxwell was New York City in 1901. She trained as a ballet dancer and joined the Metropolitan Opera Ballet in 1919. In 1924, she married Raymond J. Maxwell and shortly after began her first foray into the fashion world as a model for a wholesale company, eventually starting to sketch, design and model her own clothes in 1929. By 1936 Vera's designs were receiving positive reviews in the fashion press and her freelance collections were receiving attention in New York. She was also designing for 7th Avenue firms such as Adler and Adler, Glenhunt and Max Milstein. Vera opened Vera Maxwell Originals in 1946 and became known for her classic separates and suits, dresses teamed with jackets, print dresses, Chesterfield coats and Wraparound jersey dresses. During the war years, she gained a reputation for her practical sense, which was evident in the one-piece overalls she designed for female factory workers. Her simple lapel-less suits were spare and gave no hint of having been stripped of their decorations, which demonstrated her skill at overcoming the fabric rations of the time. Other innovations Vera became known for were her desire to design for women of short height, or without a model's figure and for women whose jobs took them beyond their desks. In 1974, she designed a "speedsuit" made of jersey with an elasticized waist, which could be pulled on in just 17 seconds. Vera closed her business in 1985, eventually passing away in January 1995."Vera Maxwell was a designer of American Classics. She started in the coat business. Nancy White, then working at Home and Garden, asked her to do children's coats for the magazine, which she did. She later got into women's fashion. “Vera’s a designer who understands women and understands their needs. There is a timelessness about her clothes, as we know, and thank heaven there is.”" - Kathy TankusThird interview in a series of three of Vera Maxwell, possibly conducted by John Touhey

    Project of the Fashion Industries Oral History Series: Vera Maxwell Part 1

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    Vera Maxwell was New York City in 1901. She trained as a ballet dancer and joined the Metropolitan Opera Ballet in 1919. In 1924, she married Raymond J. Maxwell and shortly after began her first foray into the fashion world as a model for a wholesale company, eventually starting to sketch, design and model her own clothes in 1929. By 1936 Vera's designs were receiving positive reviews in the fashion press and her freelance collections were receiving attention in New York. She was also designing for 7th Avenue firms such as Adler and Adler, Glenhunt and Max Milstein. Vera opened Vera Maxwell Originals in 1946 and became known for her classic separates and suits, dresses teamed with jackets, print dresses, Chesterfield coats and Wraparound jersey dresses. During the war years, she gained a reputation for her practical sense, which was evident in the one-piece overalls she designed for female factory workers. Her simple lapel-less suits were spare and gave no hint of having been stripped of their decorations, which demonstrated her skill at overcoming the fabric rations of the time. Other innovations Vera became known for were her desire to design for women of short height, or without a model's figure and for women whose jobs took them beyond their desks. In 1974, she designed a "speedsuit" made of jersey with an elasticized waist, which could be pulled on in just 17 seconds. Vera closed her business in 1985, eventually passing away in January 1995."Vera Maxwell was a designer of American Classics. She started in the coat business. Nancy White, then working at Home and Garden, asked her to do children's coats for the magazine, which she did. She later got into women's fashion. “Vera’s a designer who understands women and understands their needs. There is a timelessness about her clothes, as we know, and thank heaven there is.”" - Kathy TankusThird interview in a series of three of Vera Maxwell, possibly conducted by John Touhey

    Incorporação de Aloe vera em membranas de celulose bacteriana afeta a proliferação de fibroblastos e queratinócitos

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    Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia Química, Florianópolis, 2014.A celulose bacteriana (CB) é um polímero produzido por diversas bactérias, entre elas a bactérias do gênero Gluconacetobacter. Este polímero apresenta-se como uma rede de nanofibras com propriedades físico-químicas peculiares, o que o possibilita a ser utilizado como biomaterial em diversas áreas. Este biomaterial pode ter o seu espectro de aplicação ainda mais ampliado quando produzido com a incorporação de frações de extratos naturais. A Aloe vera (Aloe barbadensis Miller) é uma planta que apresenta propriedades terapêuticas e medicinais utilizadas há milhares de anos. Ela possui polissacarídeos em sua composição que atuam fortemente na regeneração e reparo de tecidos lesionados. O objetivo deste trabalho foi avaliar o comportamento das células de fibroblasto e queratinócito quando cultivadas na superfície mais porosa de membranas de CB pura e membranas de CB com incorporação de frações de Aloe vera (CB-Aloe), na perspectiva de se desenvolver futuramente um biomaterial que atue como substituto de pele humana. Por Microscopia Eletrônica de Varredura (MEV) mostrou-se que a incorporação de Aloe vera nas membranas CB não alterou a estrutura da rede nanofibras de CB pura. A análise por espectroscopia de infravermelho por refletância total atenuada (FTIR-ATR) identificou diferenças referentes aos grupos amino existentes nas frações de Aloe vera, bem como a presença de anéis piranosídicos, tais como de ß-D-manose e de ß-D-glicose. Foi possível identificar que células epiteliais quando cultivadas sobre a superfície porosa das membranas de CB com incorporação de duas frações específicas de Aloe vera (CB-G e CB-T) apresentaram atividade metabólica e proliferação celular mais expressiva do que as demais membranas estudadas. A biossíntese de colágeno por fibroblastos foi significantemente aumentada nas membranas de CB-Aloe quando comparada com a CB pura. A análise morfológica por MEV de células cultivadas sobre as membranas modificadas (CB-Aloe) mostraram que células epiteliais aderiram em todas as membranas, mas só foi observada que células cultivadas em CB pura e em CB com a incorporação de uma fração específica de Aloe vera (CB-F), estavam espraiadas indicando um efeito positivo dessa fração de Aloe vera na adesão celular.Abstract : Bacterial cellulose (BC) is a polymer produced by various bacteria, including the bacterium of the Gluconacetobacter genre. It is synthesized as a network of nanofibers with particular physicochemical properties, which make it a suitable biomaterial for the use in a variety of fields. The range of application of this biomaterial can be further expanded by the incorporation of natural extracts with biological activities. Aloe vera (Aloe barbadensis Miller) is a plant with well-documented medicinal and therapeutic properties, particularly active in regeneration and repair of injured tissues, mainly due to its particular polysaccharide composition. The objective of this study was to evaluate the behavior of keratinocyte and fibroblast cells when grown on the porous surface of pure BC membranes or on BC membranes incorporated with Aloe vera fractions (BC-Aloe), in view of the development of a biomaterial that could be used as human skin substitute. Scanning Electron Microscopy (SEM) analysis showed that the incorporation of Aloe vera into the BC nonofibers did not alter the original structure of the fiber network. Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) analysis identified differences in amino groups present in the membranes with or without Aloe vera, as well as the presence of pyranoside rings, such as ß-D-mannose and ß-D-glucose specifically in the BC-Aloe membranes. Metabolic activity and cell proliferation were more expressive when epithelial cells were cultured over the porous surface of BC membranes incorporated with two specific Aloe vera fractions (BC-T and BC-G). Biosynthesis of collagen was increased in fibroblasts cultivated on BC-Aloe membranes when compared to those cultivated on pure BC membranes. Morphological SEM analysis of cells cultivated over the modified membranes showed that epithelial cells adhered to all membranes, but spreading was only observed for cells grown on pure BC or BC incorporated with one specific Aloe vera fraction (BC-F), indicating a positive effect of this Aloe vera fraction on the cellular adhesion of the biomaterial

    Vera Kelsey Papers, 1944-1958

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    An accomplished journalist and author, Vera Kelsey's papers document her writing career through the manuscripts and research notes for her last four books, British Columbia Rides a Star, Red River Runs North!, Tomorrow is for You, and Young Men So Daring. For British Columbia Rides a Star it includes her travel notes from four trips around British Columbia

    Edgar Vera

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    Various family members carry the body of Edgar Vera, a Peruvian national, to a Catholic church to celebrate a funeral mass. Vera passed away on the 19th of August after suffering a heart attack on August 4th

    Taylor, Vera.

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    Taylor, Vera. Interview about growing up in Grand Falls-Windsor, community entertainment, and and her work with the Avalon Telephone Company. Vera Taylor discusses her family, growing up in Grand Falls-Windsor, community entertainment, her parents’ work in the mill, school, church, local businesses, police force, fires, holidays, and her work with the Avalon Telephone Company
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