2,759 research outputs found
Differential stability of 2'F-ANA*RNA and ANA*RNA hybrid duplexes: roles of structure, pseudohydrogen bonding, hydration, ion uptake and flexibility
No full text14 pags., 7 figs., 3 tabs.Hybrids of RNA with arabinonucleic acids 2′F-ANA and ANA have very similar structures but strikingly different thermal stabilities. We now present a thorough study combining NMR and other biophysical methods together with state-of-the-art theoretical calculations on a fully modified 10-mer hybrid duplex. Comparison between the solution structure of 2′F-ANA•RNA and ANA•RNA hybrids indicates that the increased binding affinity of 2′F-ANA is related to several subtle differences, most importantly a favorable pseudohydrogen bond (2′F-purine H8) which contrasts with unfavorable 2′-OH-nucleobase steric interactions in the case of ANA. While both 2′F-ANA and ANA strands maintained conformations in the southern/eastern sugar pucker range, the 2′F-ANA strand's structure was more compatible with the A-like structure of a hybrid duplex. No dramatic differences are found in terms of relative hydration for the two hybrids, but the ANA•RNA duplex showed lower uptake of counterions than its 2′F-ANA•RNA counterpart. Finally, while the two hybrid duplexes are of similar rigidities, 2′F-ANA single strands may be more suitably preorganized for duplex formation. Thus the dramatically increased stability of 2′F-ANA•RNA and ANA•RNA duplexes is caused by differencesin at least four areas, of which structure and pseudohydrogen bonding are the most important. © The Author(s) 2010. Published by Oxford University Press.Spanish Ministerio de Ciencia e Innovacion (grants
CTQ2007-68014-C02-02 to CG and BIO2009-10964 to
MO); Fundacion Marcelino Botin (grant to MO);
Canadian Institutes for Health Research (grant to
M.J.D.); Natural Sciences and Engineering Research
Council of Canada (postgraduate scholarship to
J.K.W.). Funding for open access charge: Canadian
Institutes for Health Research
Viajantes ex-cêntricas nas histórias de Ana Miranda
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão. Programa de Pós-Graduação em Literatura.No conjunto das narrativas ficcionais da escritora brasileira Ana Miranda, a temática da viagem # considerada uma das mais férteis da literatura ocidental de todos os tempos # ocupa um espaço de centralidade, podendo até mesmo ser vista como o mais importante eixo de estruturação de suas obras. O estudo realizado pela presente tese tem por objetivo analisar o tratamento dado ao tema da viagem nos romances Desmundo, O retrato do rei, Dias & Dias e Amrik, evidenciando que por intermédio das narradora-viajantes # Oribela, Mariana, Feliciana e Amina # a autora promove um diálogo entre diferentes culturas, gêneros, etnias e gerações, ao mesmo tempo em que estabelece um profícuo diálogo com o passado em sua invariante problematização concernente aos limites e cruzamentos entre o discurso ficcional e os discursos narrativos extraliterários que o cercam, sobretudo o histórico e o biográfico. In the whole of Brazilian writer Ana Miranda#s fictional narrative, the travel thematic # considered one of the most fertile themes of the western literature of all times # occupies a central space, and can even been seen as the most important structuring axis of Miranda#s works. The aim of the present thesis is to analyze the treatment given to the travel subject in the novels Desmundo, O retrato do rei, Dias & Dias and Amrik, emphasizing that, through the traveling narrators, Oribela, Mariana, Feliciana and Amina, the author promotes a connection between different cultures, genders, ethnics and generations, at the same time that establishes a profitable dialogue with the past in her invariant problematization concerning the limits and crossings between the fictional and the extra-literary narrative discourses that surrounds it, mainly the historical and the biographic ones
As mulheres do império: uma leitura de Ana de Amsterdam / Women’s Empire: A Reading of Ana de Amsterdam
No full textResumo: Enquadrado no bojo da produção identificada como “literatura dos retornados”, o interesse principal de Ana de Amsterdam (2016a), de Ana Cássia Rebelo, não recai nas imagens traumáticas do retorno ou na violência praticada entre colonizadores e colonizados, como é recorrente no gênero. De forma até sintomática, as lembranças de África são esporádicas na menina de cinco anos que deixou Moçambique junto à família. Em seu lugar, a exuberância de uma Índia portuguesa sonhada e projetada por ela ocupam as lacunas de um presente insatisfatório, dividido entre a criação dos três filhos de um casamento em crise e o emprego burocrático desempenhado numa Lisboa pouco atrativa. Em ambos, tanto na Goa portuguesa como no trajeto para o trabalho, despontam narrativas de mulheres que constituem a síntese entre o diário íntimo de Ana e a escrita testemunhal da diáspora. Numa primeira parte do estudo, recupera-se a gênese do romance no formato do blog assinado pela autora, evidenciando a “escrita do eu”, nos moldes dos estudos de autobiografias, diários e afins. O segundo momento volta-se para a escrita testemunhal no lastro da narrativa pós-colonial e também da pós-memória. Em comum, os dois planos tratam da perspectiva feminina, seja na batalha contemporânea da cosmopolita Lisboa, seja nos desdobramentos silenciados do pós-colonialismo, em meio às histórias duplicadas de outras tantas Anas.Palavras-chave: Ana de Amsterdam; Ana Cássia Rebelo; diário íntimo; literatura de testemunho; blogs.Abstract: Framed in the center of the production identified as “literature of the returnees”, the main focus of Ana de Amsterdam (2016a) by Ana Cássia Rebelo, does not lie in the traumatic images of the return or in the violence practiced between colonizers and colonized, as it is usually the case in this genre. Somehow, even symptomatically, African memories are sporadic in the five-year-old girl who left Mozambique with her family. Instead, the exuberance of a Portuguese India, dreamed and projected by her, occupies the gaps of an unsatisfactory present, dividing herself to raise three children of a marriage in crisis and work in the bureaucratic employment situated in an unattractive Lisbon. In both, Portuguese Goa and on the way to work, narratives of women emerge and represent the synthesis between Ana’s private diary and the testimonial writing of the diaspora. In a first part of the study, the genesis of the novel is recovered in the form of a blog signed by the author, emphasizing the “writing of the self”, in the molds of autobiographies, journals and etc. The second moment turns to the testimonial writing in the basis of the postcolonial narrative and also of the post-memory. In common, the two plans deal with the feminine perspective, whether in the contemporary battle of cosmopolitan Lisbon or in the silenced developments of postcolonialism, in the middle of the duplicate stories of so many Anas.Keywords: Ana de Amsterdam; Ana Cássia Rebelo; diary; testimonial literature; blogs
Neither author, not only function-author : but also idealizer-subject
No full textO trabalho apresenta o desenvolvimento de uma noção proposta em nossa Dissertação, mas não desenvolvida até o momento. Trata-se de função-idealizador. A partir de reflexões sobre sujeito, função-autor e função-leitor, dentro do Projeto ‘História das Ideias’, propomos algumas análises sobre constituição, formulação e circulação de sentidos e explicitamos, levando em conta a teoria e as análises, o desenvolvimento de nossas questões acerca da noção de função-autor.The paper presents the development of a notion proposed in our master’s research but not yet concluded. It is the notion of idealizer-function. From reflections on subjects like function of author and function-of reader, inside the Project ‘History of Ideas’ we develop some analysis on the constitution, formulation and circulation of senses, considering our questions on the notion of author-function
Determining the need for an expanded fitness component in the Fire Science Program at Milwaukee Area Technical College
No full textIncludes bibliographical references
Does strict employment protection discourage job creation? Evidence from Croatia
No full textEmployment protection legislation in Croatia is among the most strict in Europe. Firing is difficult and costly, and flexible forms of employment are limited. Is this apparent rigidity reflected-as one would expect based on standard economic theory-in low labor market dynamics? Is job creation low and hiring limited? Is the job security of insiders achieved at the cost of outsiders not being able to enter thelabor market? The author attempts to answer these questions by examining job flows. If the employment protection legislation is binding, then job and worker turnover should be low. He shows that this is indeed the case. Hiring is limited and the average job tenure is very long in Croatia. Job destruction is low, however job creation is still lower. The result is accumulation of unemployment, in large part due to new labor market entrants not being able to find a job. The high degree of job protection also seems to strengthen the bargaining position of insiders and results in relatively high wages. So, wages in Croatia are higher than among its competitors, even after adjusting for productivity. These high labor costs are likely to contribute to limited job creation in existing firms, but also are likely to discourage the entry of-and thus job creation in-new firms. The author presents evidence that firm growth has been indeed limited in Croatia, contributing to the low employment level. The author examines other potential causes of high unemployment in Croatia (the unemployment benefit system, labor taxation, the wage structure, and skill and spatial mismatches). He argues that they do not play a substantial part in accounting for poor labor market outcomes in Croatia. The author concludes that the stringent employment protection legislation is the key labor market institution behind low job creation and high unemployment. Based on this he recommends specific measures aimed at liberalizing the labor market to foster job creation and employment.Labor Management and Relations,Labor Policies,Labor Markets,Environmental Economics&Policies,Trade Finance and Investment,Labor Markets,Labor Management and Relations,Labor Standards,Banks&Banking Reform,Environmental Economics&Policies
El Cerrito: Queretaro
No full textLa información de esta miniguía , se basa a la fecha de publicación de la misma, en los trabajos de la arqueóloga Ana María CrespoEste antiguo centro fue cabecera de un importante poblado de relevancia regional que tuvo su desarrollo hacia el primer milenio de nuestra era, fue contemporáneo de Teotihuacan y posteriormente de Tula, las dos grandes capitales del centro de México. El Cerrito domina una zona agrícola que es considerada como la entrada al Bajío, región rica en flora y fauna, recursos básicos para la economía prehispánica. El sitio se encuentra ubicado en el eje donde convergen los caminos que unen el centro con el norte y occidente de México.</p
The Personality of Ana Ozores
No full textHOUDKOVÁ, Sandra: Osobnost Any Ozores [Bakal愃Ā爃ఀsk愃Ā pr愃Āce]. - Univerzita Karlova. Filozofick愃Ā fakulta; 唃Āstav rom愃Ānsk礃Āch studi椃Ā. Hispanistika. - vedouc椃Ā pr愃Āce: doc. Juan Antonio Sánchez Fernández, Ph.D. La Regenta je mistrovsk礃Ām d椃Ālem španělského autora Leopolda Alase a p爃ఀedmětem zkoum愃Ān椃Ā této bakal愃Ā爃ఀské pr愃Āce. V prvn椃Ā č愃Āsti se zamě爃ఀ椃Ām na autora tohoto d椃Āla, zasad椃Ām d椃Ālo do historického kontextu, objasn椃Ām ž愃Ānr a p爃ఀibl椃Āž椃Ām roli ženy v 19. stolet椃Ā, abych p爃ఀibl椃Āžila postaven椃Ā protagonistky d椃Āla, Any Ozores, které se věnuje anal礃Āza druhé č愃Āsti této pr愃Āce. Anal礃Āza se nejd爃ఀ椃Āve zamě爃ఀ椃Ā na postavu samotnou a poté bude zkoumat č愃Āsti jej椃Āho života a lidi, které ji ovlivnili. P爃ఀesněji se soust爃ఀed椃Ā na jej椃Ā dětstv椃Ā, romantick礃Ā život, v椃Āru a společnost města, ve kterém Ana žije. Kl椃Āčové slova v češtině: La Regenta, Clarín, naturalismus, realismus, osobnost Any Ozores, psychologieHOUDKOV䄃Ā, Sandra: Osobnost Any Ozores [Bakal愃Ā爃ఀsk愃Ā pr愃Āce]. - Univerzita Karlova. Filozofick愃Ā fakulta; 唃Āstav rom愃Ānsk礃Āch studi椃Ā. Hispanistika. - vedouc椃Ā pr愃Āce: doc. Juan Antonio S愃Ānchez Fern愃Āndez, Ph.D. La Regenta is the masterpiece of the Spanish author Leopoldo Alas and the subject of this bachelor thesis. In the first part, I will focus on the author of this work, place the work in its historical context, explain the genre, and introduce the role of women in the 19th century in order to bring into focus the position of the protagonist of the work, Ana Ozores, who is the focus of the analysis of the second part of this thesis. The analysis will first focus on the character herself and then examine parts of her life and the people who influenced her. More specifically, it will focus on her childhood, romantic life, faith, and the society of the city in which Ana lives. Keywords in English: La Regenta, Clarín, naturalism, realism, personality Any Ozores, psychologyInstitute of Romance StudiesÚstav románských studiíFilozofická fakultaFaculty of Art
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