6 research outputs found

    Complex scenographic design of Jean Genet's drama

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    V této diplomové práci se pokusím detailně rozebrat nejen samotný text Jeana Geneta Služky, ale i specifika samotného autora a celkově jeho tvorby. Pokusím se pochopit Geneta jako člověka. V této práci nechci odpovídat na otázky, ale chci prozkoumat, co s čím souvisí, co se jak vyvíjí a případnému čtenáři předložit podklady k zamyšlení. Dále chci rozebrat témata související s materiálem, o kterém píšu. Pokusím se charakterizovat různé pohledy na jedno dílo.In this thesis I will try to analyse in detail not only the text of Jean Genet's The Handmaid's Tale, but also the specifics of the author himself and his work in general. I will try to understand Genet as a person. I do not want to answer questions in this thesis, but I want to explore what is related to what, how it develops and provide food for thought for the potential reader. I also want to discuss themes related to the material I am writing about. I will attempt to characterize different perspectives on one work

    Carbon target as neutron source from 12C(d,n)13N and D(d,n)3He reactions

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    Possibility to use carbon as a target material to generate fast neutrons from D(d,n)3He nuclear reaction and intermediate energy neutrons from 12C(d,n)13N nuclear reaction was studied. The experiment results were obtained to characterize an adsorption of the deuterium while stuffing the carbon target by beam of accelerated ions and a desorption of the deuterium from the same carbon target as the function of its temperature. Bombardment of carbon target with 400 keV deuteron beam of 1 mA current leads to the 12C(d,n)13N nuclear reaction, allowing to generate neutrons of (10 ÷ 100) keV energy range with neutron flux density (106 - 107) n/(cm2·s) near the carbon target

    Growing a circular economy with fungal biotechnology:a white paper

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    Fungi have the ability to transform organic materials into a rich and diverse set of useful products and provide distinct opportunities for tackling the urgent challenges before all humans. Fungal biotechnology can advance the transition from our petroleum-based economy into a bio-based circular economy and has the ability to sustainably produce resilient sources of food, feed, chemicals, fuels, textiles, and materials for construction, automotive and transportation industries, for furniture and beyond. Fungal biotechnology offers solutions for securing, stabilizing and enhancing the food supply for a growing human population, while simultaneously lowering greenhouse gas emissions. Fungal biotechnology has, thus, the potential to make a significant contribution to climate change mitigation and meeting the United Nation's sustainable development goals through the rational improvement of new and established fungal cell factories. The White Paper presented here is the result of the 2nd Think Tank meeting held by the EUROFUNG consortium in Berlin in October 2019. This paper highlights discussions on current opportunities and research challenges in fungal biotechnology and aims to inform scientists, educators, the general public, industrial stakeholders and policymakers about the current fungal biotech revolution.</p

    VEuPathDB: the eukaryotic pathogen, vector and host bioinformatics resource center

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    The Eukaryotic Pathogen, Vector and Host Informatics Resource (VEuPathDB, https://veupathdb.org) represents the 2019 merger of VectorBase with the EuPathDB projects. As a Bioinformatics Resource Center funded by the National Institutes of Health, with additional support from the Welllcome Trust, VEuPathDB supports &gt;500 organisms comprising invertebrate vectors, eukaryotic pathogens (protists and fungi) and relevant free-living or non-pathogenic species or hosts. Designed to empower researchers with access to Omics data and bioinformatic analyses, VEuPathDB projects integrate &gt;1700 pre-analysed datasets (and associated metadata) with advanced search capabilities, visualizations, and analysis tools in a graphic interface. Diverse data types are analysed with standardized workflows including an in-house OrthoMCL algorithm for predicting orthology. Comparisons are easily made across datasets, data types and organisms in this unique data mining platform. A new site-wide search facilitates access for both experienced and novice users. Upgraded infrastructure and workflows support numerous updates to the web interface, tools, searches and strategies, and Galaxy workspace where users can privately analyse their own data. Forthcoming upgrades include cloud-ready application architecture, expanded support for the Galaxy workspace, tools for interrogating host-pathogen interactions, and improved interactions with affiliated databases (ClinEpiDB, MicrobiomeDB) and other scientific resources, and increased interoperability with the Bacterial &amp; Viral BRC

    Aplicación de la economía circular en el proceso de cultivo de macrohongos en subproductos del café

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    Figuras, tablasLos macrohongos contienen enzimas hidrolíticas (celulosas y hemicelulosas) y oxidativas (lacasas y peroxidosas) que permiten degradar y acelerar los procesos de descomposición de la materia orgánica, como los residuos de origen agrícola, contribuyendo al ciclo del carbono. Además, los macrohongos forman estructuras reproductivas visibles que cumplen funciones claves en el ciclo de vida del hongo y que, a su vez, pueden tener un valor biotecnológico importante para la sociedad. Por lo tanto, la comprensión del ciclo de vida y los requerimientos ambientales de los macrohongos pueden contribuir a la descomposición de residuos agrícolas como los subproductos del cultivo del café (Coffea arabica) y a la producción de biomasa con potencial medicinal, nutricional o biorremediador. En este sentido, en esta investigación exploratoria, se buscó evaluar la viabilidad del cultivo de tres especies de macrohongos: Ganoderma lucidum, Pleurotus ostreatus y Coprinus sp, en subproductos del café, principalmente del pericarpio del fruto (pulpa) que es el principal subproducto del beneficio del café. Para ello, se buscó evaluar las condiciones ambientales de humedad, pH, luminosidad y temperatura óptimos para cada una de las fases del cultivo de los hongos mencionados bajo una lógica de economía circular sustentable. Los resultados mostraron que la adecuación del sustrato óptima a base de pulpa para G. lucidum fue con fermentación sumergida de 10 días con peróxido de hidrógeno al 1 % y esterilización con autoclave por 20 minutos con el cual se obtuvo una eficiencia biológica de 5,8 %. Por otro lado, se encontró que es posible el crecimiento de P. ostreatus en residuos del cultivo de G. lucidum con tiempos más prolongados de crecimiento. Además, no se observó colonización completa de Coprinus sp. en pulpa de café fermentada 10 días o lavada, lo cual podría indicar que se necesita períodos más extensos de degradación de la pulpa para su crecimiento. En conclusión, G. lucidum es una especie que tiene un crecimiento potencial en la pulpa del café, lo cual puede aportar significativamente a la valorización de los residuos del café y a la producción de setas medicinales a través de un sistema de economía circular sustentable.Macrofungi contain hydrolytic (cellulose and hemicellulose) and oxidative (laccase and peroxidases) enzymes that degrade and accelerate the decomposition of organic matter, such as agricultural waste, contributing to the carbon cycle. Furthermore, macrofungi form visible reproductive structures that play key roles in the fungal life cycle and, in turn, can have significant biotechnological value for society. Therefore, understanding the life cycle and environmental requirements of macrofungi can contribute to the decomposition of agricultural waste, such as coffee (Coffea arabica) by-products, and to the production of biomass with medicinal, nutritional, or bioremediation potential. In this exploratory study, we look into to evaluate the viability of cultivating three species of macrofungi: Ganoderma lucidum, Pleurotus ostreatus, and Coprinus sp., in coffee by-products, primarily the fruit pericarp (pulp), which is the main by-product of the coffee processing industry. To this end, we look into to evaluate the optimal environmental conditions of humidity, pH, light, and temperature for each of the cultivation phases of the aforementioned fungi with a sustainable circular economy framework. The results showed that the optimal pulp-based substrate for G. lucidum was a 10-day submerged fermentation with 1% hydrogen peroxide and 20-minute autoclave sterilization, obtaining a biological efficiency of 5.8%. Furthermore, we found that P. ostreatus can grow on G. lucidum crop residues with longer growth times. Furthermore, complete colonization of Coprinus sp. was not observed in fermented or washed coffee pulp for 10 days, which could indicate that longer pulp degradation periods are required for its growth. In conclusion, G. lucidum is a specie with potential for growth in coffee pulp, which can significantly contribute to the valorization of coffee waste and the production of medicinal mushrooms through a sustainable circular economy system.Introducción -- Planteamiento del problema -- Justificación -- Marco teórico y antecedentes -- Cultivo de Ganoderma lucidum -- Sustratos -- Rendimientos del proceso -- Condiciones ambientales de operación -- Factores nutricionales -- Costos -- Cultivo de Pleurotus ostreatus -- Condiciones del sustrato -- Condiciones ambientales -- Cultivo de Coprinus sp -- Condiciones ambientales -- Materiales y métodos -- Aislamiento de las cepas -- Activación de las cepas -- Producción de inóculo -- Elaboración del sustrato -- Incubación -- Fructificación -- Cosecha -- Resultados y discusión -- Aislamiento y activación de cepas -- Producción de inóculo e incubación -- Fructificación y cosecha -- Selectividad biológica de macrohongos -- Conclusiones -- Referencias.PregradoBiólogo(a)Universidad de CaldasMicologí
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