59 research outputs found
Genome Sequencing of Strain Vm263, a Hypotoxic Strain of Valsa mali Causing Apple Valsa Canker
No full textApple valsa canker, caused by Valsa mali, has seriously endangered the sustainable development of the apple industry in China. Our previous study revealed that low-temperature induction increased the pathogenicity of strain Vm263, which is a hypotoxic strain of V. mali causing apple valsa canker. However, the regulatory mechanism of this phenomenon was still unclear. In this study, whole genome sequencing of strain Vm263 was performed using a combination of long-read single-molecule real-time sequencing technology (Pacific Biosciences) and MGISEQ sequencing. The genome size of strain Vm263 was 42.83 Mb and the assembly N50 was 5.09 Mb. The genome provides a valuable resource for facilitating our understanding of the response of V. mali to low temperature. [Figure: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license
O TEOR REVELADOR DO TÍTULO E DAS EPÍGRAFES EM DE PROFUNDIS, VALSA LENTA
No full textThis article presents an analisys of the title and the epigraphs found in the book De Profundis Valsa Lenta by the Portuguese author José Cardoso Pires, aiming at verifying the importance of these elements which bring light not only to the entire book through its title, but also to each chapter begun with an epigraph.Este artigo apresenta uma análise do título e das epígrafes encontradas na obra De Profundis Valsa Lenta do autor português José Cardoso Pires, visando à verifi cação da importância desses elementos que trazem luz não só ao livro inteiro através do título, mas, também, a cada capítulo iniciado com uma epígrafe
UM FAUSTO BRASILEIRO? UMA ANÁLISE INTERTEXTUAL DE VALSA PARA BRUNO STEIN
No full textEste artigo consiste numa análise crítica intertextual do romance Valsa para Bruno Stein (1986), do escritor gaúcho Charles Kiefer, cuja narrativa apresenta desde o início diversas citações do livro Fausto (1808), do escritor alemão Johann Wolfgang von Goethe. O objetivo deste trabalho foi procurar compreender o efeito de sentido dessas citações, que podem ser consideradas pistas para outras interpretações possíveis, certamente diferentes daquelas que ignoram os elementos intertextuais recorrentes no texto. Considerando as referências à obra de Goethe como um importante elemento intertextual deslocado, analisou-se qual a relação existente entre ambas as narrativas, que significados as citações de Fausto produzem no romance em questão e se estas ratificam ou subvertem a narrativa goethiana.A BRAZILIAN FAUST? AN INTERTEXTUAL ANALYSIS OF VALSA PARA BRUNO STEINThis paper is an intertextual analysis of the Brazilian book Valsa para Bruno Stein (1986), written by the Gaucho author Charles Kiefer, which narrative presents, since the beginning, many quotations from the book Faust (1808), written by the German author Johann Wolfgang von Goethe. The aim of this work is to comprehend the meaning of these quotations in this context, which can be considered like clues to other possible interpretations, certainly different from that ones that ignore these intertextual elements. Considering these references to Faust as an important intertextual dislocated element, it was analyzed what was the relationship between both narratives, which meanings were produced by these quotations in the Brazilian book and if these quotations confirm or subvert the Goethean narrative
Um olhar sobre a dramaturgia e três propostas de direção de valsa nº 6
No full textO nosso objeto de estudo é a peça Valsa n° 6, de Nelson Rodrigues. Inicialmente, discorre-se acerca do contexto de criação da obra a partir da peça Vestido de Noiva, de Nelson Rodrigues, quando o teatro brasileiro passa por modificações estruturais, tanto no que diz respeito à dramaturgia quanto à interpretação e à direção. Investigam-se os elementos que influenciaram na construção do enredo de Valsa n° 6 e a sua primeira montagem. A seguir, examina-se a direção de três montagens da peça, incluindo a de estreia, em 1951. Utiliza-se como fonte de pesquisa obras a respeito do autor, da peça, e, no que se refere à direção, fontes que descrevem as montagens de 1951, de 2012 e de 2017. O propósito visa alcançar um conhecimento significativo da obra do dramaturgo Nelson Rodrigues e das propostas de montagem a que tivemos acesso.Our object of study is the piece Valsa n° 6, by Nelson Rodrigues. Initially, the context of creation of the work is discussed based on the play Vestido de Noiva, by Nelson Rodrigues, when Brazilian theater undergoes structural changes, both in terms of dramaturgy and interpretation and direction. The elements that influenced the construction of the plot of Waltz n° 6 and its first montage are investigated. Next, the direction of three stagings of the play is examined, including the premiere, in 1951. As a source of research, works about the author, the play, and, with regard to the direction, sources that describe the stagings of 1951, 2012 and 2017. The purpose is to achieve a significant knowledge of the playwright Nelson Rodrigues' work and the staging proposals to which we had access
Gα proteins Gvm2 and Gvm3 regulate vegetative growth, asexual development, and pathogenicityon apple in Valsa mali.
No full textIn fungi, heterotrimeric guanine-nucleotide binding proteins (G-proteins) are key elements of signal transduction pathways, which control growth, asexual and sexual development, as well as virulence. In this study, we have identified two genes encoding heterotrimeric G protein alpha subunits, named Gvm2 and Gvm3, from Valsa mali, the causal agent of apple Valsa canker. Characterization of Gvm2 and Gvm3 mutants indicates that Gvm3 may be a crucial regulator of vegetative growth. Deletion of the corresponding gene results in a 20% reduction in growth rate. Besides, Gvm2 and Gvm3 seem to be involved in asexual reproduction, and mutants are hypersensitive to oxidative and cell membrane stresses. Interestingly, both G protein alpha subunits were most probably involved in V. mali virulence. In infection assays using Malus domestica cv. 'Fuji' leaves and twigs, the size of lesions caused by deletion mutants △Gvm2, or △Gvm3 are significantly reduced. Furthermore, many genes encoding hydrolytic enzymes-important virulence factors in V. mali-are expressed at a lower level in these deletion mutants. Our results suggest that Gvm2 and Gvm3 play an important role in virulence probably by regulation of expression of cell wall degrading enzymes. △Gvm2, and △Gvm3 mutants were further analyzed with respect to their impact on the transcript levels of genes in the cAMP/PKA pathway. The expression of the genes encoding adenylate cyclase VmAC, protein kinase A (PKA) regulatory subunit VmPKR, and PKA catalytic subunit VmPKA1 are down-regulated in both mutants. Further analyses indicated that intracellular cAMP level and PKA activity are down-regulated in the △Gvm3 mutant, but are basically unchanged in the △Gvm2 mutant. Overall, our findings indicate that both Gvm2 and Gvm3 play diverse roles in the modulation of vegetative growth, asexual development, and virulence in V. mali
“Think Research” in Everyday Clinical Practice: Fostering Research Culture in Health Care Settings
No full text“No development without research; No research without development” Yvo Nuyens1TThe quest for knowledge has helped mankind evolve over the years, from discovery of fire through Dolly the sheep clone to Chandrayan moon mission, leaving behind a record of discoveries and inventions in science and technology. Each generation can begin the search for knowledge where the last one left off and contribute their share to the benefit of humanity, cutting across culture and boundaries
Think Research" in Everyday Clinical Practice: Fostering Research Culture in Health Care Setting
No full textA valsa nos trópicos: repercussões de um fenômeno europeu na literatura brasileira do século XIX
No full textResumo: Partindo de uma observação feita na obra Raízes do Brasil (1936), de Sérgio Buarque de Holanda, na qual o autor reflete sobre a disparidade entre a sociedade brasileira do século XIX e as ideias do liberalismo europeu, Roberto Schwarz desenvolve em Ao vencedor as batatas (1977) uma crítica ao Romantismo brasileiro, que teria sido montado sobre uma comédia ideológica, diferente da europeia, representando uma idealização que não correspondeu à realidade. O presente artigo revê esses conceitos a partir da cena de baile da obra Senhora (1875), de José de Alencar. Observa-se a presença da valsa na literatura brasileira oitocentista, objetivando analisar de que maneira esse fenômeno europeu se manifestou em território brasileiro e como o imaginário em torno do baile foi apropriado e relido pelo autor. O artigo coloca a literatura brasileira em diálogo com a literatura europeia produzida no mesmo período. Ao ponto de vista de Roberto Schwarz acrescenta-se o estudo feito por Maria Cecília de Moraes Pinto (1999) que investiga as influências europeias de Alencar e o seu empenho em fazer uma literatura nacional.Palavras-chave: valsa na Europa e no Brasil; Romantismo; literatura nacional.Abstract: The piece of writing Raízes do Brasil (1936) by Sérgio Buarque de Holanda, in which the author reflects about the disparities between Brazilian society of the nineteenth century and the ideas of European liberalism, provides a basis to Roberto Schwarz’s criticism of Brazilian Romanticism in Ao vencedor as batatas (1977). According to Schwarz, different from its European correlate, Brazilian Romanticism would have been based on an ideological comedy, thus representing an idealization that did not correspond to reality. This article revisits such concepts from the perspective of the ballroom dancing scene in Senhora (1875), by José de Alencar. Based on the presence of the waltz in nineteenth century Brazilian literature we analyzed how the European phenomenon manifested in Brazilian territory and how the imaginary constructed around ballroom dancing was appropriated and re-read by Alencar. This work establishes a connection between Brazilian Literature and the literature produced in Europe during the same period. In addition to Roberto Schwartz’s point of view, we included Maria Cecília de Moraes Pinto’s study (1999), which investigated Alencar’s European influences and his efforts in creating a national literature.Keywords: waltz in Europe and Brazil; Romanticism; national literature.</jats:p
Teaching mathematics to able children
No full textThis book enables teachers to effectively meet the needs of their most able mathematicians. Using a tried and tested set of principles developed and used by The Able Children's Education Unit at Brunel University, the author demonstrates how to: identify high mathematical ability in a pupil, plan suitably challenging activities and teach them most effectively within the existing National Numeracy framework, make the most of the classroom resources available, including ICT and external agencies, implement strategies for differentiation, illustrated with real-life classroom examples. A
Clear cell calcifying epithelial odontogenic tumor: A rare case report
No full textThe calcifying epithelial odontogenic tumor (CEOT) is a benign epithelial odontogenic lesion that accounts for less than 1% of all odontogenic tumors. Clear cell variant of CEOT is extremely rare with only fifteen cases documented till date. The occurrences of clear cells may prove to be a sign of increased tumor aggressiveness, indicating the need for a more radical surgical approach
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