11,802 research outputs found
Sources of diversity of carbapenem resistance levels in Klebsiella pneumoniae carrying blaVIM-1
No full textObjectives: To elucidate the mechanisms responsible for the diversity of β-lactam resistance phenotypes among isolates of a VIM-1-producing Klebsiella pneumoniae (VPKP) strain that is endemic in Greek hospitals. Methods: Five VPKP clinical isolates were studied. MICs of β-lactams were determined by agar dilution. PFGE of Xba I-digested genomic DNA was used for typing. Profiles of outer membrane proteins (OMPs) were determined by SDS-PAGE. Selected isolates were transformed with a plasmid encoding the Omp36K porin. β-Lactamase activities were analysed by IEF and imipenem hydrolysis was assessed by spectrophotometry. VIM-1-encoding, self-transmissible plasmids were characterized by replicon typing, RFLP and hybridization with bla VIM - and IS26-specific probes. Characterization of integrons was performed by PCR, cloning and sequencing. Results: Isolates exhibited highly similar PFGE patterns. Imipenem MICs were 2, 4, 16, 32 and 64 mg/L. The isolate with the highest imipenem MIC (Vipm-64) lacked a 36 kDa OMP. Expression of a cloned OmpK36 in this isolate reduced the imipenem MIC to susceptibility levels. Imipenem-hydrolysing activity was significantly higher in Vipm-16 as compared with the other isolates that expressed similar amounts of VIM-1. All isolates transferred β-lactam resistance to Escherichia coli through conjugative, IncN plasmids that exhibited differences in the RFLP and hybridization patterns with bla VIM - and IS 26 -specific probes. The Vipm-16 plasmid, mediating the higher imipenem MICs among transconjugants, carried two copies of bla VIM-1. Cloning and sequencing showed In-e541-like integrons truncated at the 5′CS by insertion of IS 26 elements at two different positions. Conclusions: A VIM-1-producing strain of K. pneumoniae has evolved through OMP alterations and rearrangements in the bla VIM-1-carrying plasmid probably mediated by IS 26, generating isolates with imipenem MICs ranging from susceptibility to resistance. © 2006 Oxford University Press
Multidrug and broad-spectrum cephalosporin resistance among Salmonella enterica serotype Enteritidis clinical isolates in southern Italy
No full textFrom 1992 to 1997, only six sporadic isolates of Salmonella enterica serotype Enteritidis from patients with cases of gastroenteritis in southern Italy exhibited resistance to broad-spectrum cephalosporins. Five isolates produced SHV-12, and one isolate encoded a class C β-lactamase. The blaSHV-12 gene was located in at least two different self-transferable plasmids, one of which also carried a novel class 1 integron
Implementasi Protokol Keamanan Dan Segmentasi Jaringan Dalam Project Pembangunan WLAN Untuk PT Pan Pacific Insurance
No full textSecurity of data and network devices is essential in the development of a computer network, either in the creation of new computer networks or in the development of existing computer networks. PT. Pan Pacific Insurance as a company that holds large amounts of data along with its supporting computer network's infrastructure, focuses on security aspects in addition to performance aspects in its computer network. To get the flexibility of its computer network, the company developed a wireless local area network (WLAN) in line with the relocation of its head office. The Existing company's network needs to be improved with a WLAN network, so it does not only improve employee performance and provides the best security for company data. For those purposes, the authors applied the use of Cisco Wireless LAN Controller (WLC), Cisco Access Point and applied the WPA+WPA2 PSK security protocol for the SSID used by employees. Using this protocol when employees want to connect to the network wireless, they will be forced to type the password that has been set. In addition to implementing the security protocol, the author also applies the Web Policy Authentication security protocol for the SSID used by guests. So that guests who want to connect to a wireless network will be directed to a web browser to be asked to enter a username and password first. A series of security protocols, also added with network segmentation on each SSID to use a different network address. Thus, the essence of network security and performance is expected to increase. From the results of the tests carried out, obtained security guarantees on the PT. Pan Pacific Insurance successfully directs every user connected to the network as part of the security protocol implemented. Furthermore, with network segmentation, user traffic is more controlled because guests get their network segmentation, so they don't interfere with network traffic for users or company staff in company operations
Titanium oxide adhesion layer for high temperature annealed Si/Si3N4/TiOx/Pt/LiCoO2 battery structures
No full textThis work describes the influence of a high annealing temperature of about
700C on the Si(substrate)/Si3N4/TiOx/Pt/LiCoO2 multilayer system for the
fabrication of all-solid-state lithium ion thin film microbatteries. Such microbatteries
typically utilize lithium cobalt oxide (LiCoO2) as cathode material
with a platinum (Pt) current collector. Silicon nitride (Si3N4) is used to act as a
barrier against Li diffusion into the substrate. For a good adherence between
Si3N4 and Pt, commonly titanium (Ti) is used as intermediate layer. However,
to achieve crystalline LiCoO2 the multilayer system has to be annealed at high
temperature. This post-treatment initiates Ti diffusion into the Pt-collector
and an oxidation to TiOx, leading to volume expansion and adhesion failures.
To solve this adhesion problem, we introduce titanium oxide (TiOx) as an
adhesion layer, avoiding the diffusion during the annealing process. LiCoO2,
Pt and Si3N4 layers were deposited by magnetron sputtering and the TiOx
layer by thermal oxidation of Ti layers deposited by e-beam technique. Asdeposited
and annealed multilayer systems using various TiOx layer thicknesses
were studied by scanning electron microscopy (SEM) and time-of-flight
secondary ion mass spectrometry (ToF-SIMS) and x-ray photoelectron spectroscopy
(XPS). The results revealed that an annealing process at temperature
of 700C leads to different interactions of Ti atoms between the layers, for
various TiOx layer thicknesses (25–45 nm).This work was financially supported by FEDER/ COMPETE and FCT funds with the Projects PTDC/ EEA-ELC/114713/2009, PEst-C/QUI/UI0686/2013 and UID/EEA/04436/2013, first author scholarship SFRH/BPD/95905/2013 and second author scholarship SFRH/BD/78217/2011. This work was carried out with the support of the Karlsruhe Nano Micro Facility (KNMF, www.kit.edu/knmf), a Helmholtz Research Infrastructure at Karlsruhe Institute of Technology (KIT, www.kit.edu)
PROSEDUR PENGAJUAN DOKUMEN PROYEK PADA PT FIBERHOME TECHNOLOGIES INDONESIA SURABAYA
No full textThe purpose of this study was to determine the submission procedure of project's documents in PT FiberHome Technologies Indonesia. The method used in this study is a direct approach, the author does not just reading and taking references from the books of the project but as a project secretary, authors also took a part in the project's document submission process. The results showed that the procedures for submission of project's documents in PT FiberHome Technologies Indonesia has been running welL. Procedures for the submission project's document in PT FiberHome Technologies Indonesia are as follows: Permitting, Preparation of drawings and material needs, Implementation, Ready for Service, Project controlling,
Acceptance Test
STRATEGI BISNIS PT. POS INDONESIA
No full textPT. Pos Indonesia is a company focusing on the delivery of packages and documents worldwide. Now we know that many courier companies and forwarding serve delivery services. So that PT. Pos Indonesia can survive amid competition, the author will formulate a strategy. But earlier, the authors need to analyze internal and external factors. The author will use a SWOT analysis to complete this study. This method is quite flexible in various state companies. The results of this study PT. Pos Indonesia are advised to use its strength to deal with the threats and opportunities. The strategies implemented by utilizing the factors of internal forces that exist to grab opportunities PT. Pos Indonesia in order to increase business. Based on the author analysis, an IFE score of 2.35 and EFE score of 2.24 and be in quadrant V, stability
Effect of Pt bottom electrode texture selection on the tetragonality and physical properties of Ba 0.8Sr 0.2TiO 3 thin films produced by pulsed laser deposition
No full textThe effect of platinum (Pt) bottom electrode texture on the tetragonality, dielectric, ferroelectric, and polarization switching response of pulsed laser deposited Ba 0.8Sr 0.2TiO 3 (BST) thin films has been studied. The x-ray diffraction and Raman analysis revealed the higher tetragonality of BST films when they were grown on higher (111) textured Pt layer. The properties like dielectric permittivity, polarization, switching time, and leakage currents were found to be correlated to tetragonality and orientation of the BST films. The polarization current was observed to be higher in BST films on Pt epitaxial layer and it exhibits exponential dependence on the electric field. The voltage-current measurements displayed Ohmic behavior of leakage current irrespective of Pt texture for low voltages (up to 1 V), whereas at higher voltages the conduction mechanism was found to be dependent on texture selection of bottom Pt electrode. © 2012 American Institute of Physics.The author J.P.B.S. thanks FCT for the financial support (Grant SFRH/BD/44861/2008). K.C.S. thanks FCT for Postdoc Grant (SFRH/BPD/68489/2010). J.M.S. thanks FCT for the financial support (Grant SFRH/BPD/64850/2009).Peer Reviewe
PROSES KOMUNIKASI DI PT INTI SELERA ASIA PADA RESTORAN BENTOYA SUPERMALL SURABAYA
No full textCommunication is a transfering process of ideas and concept between two persons or more. Communications is success when both of them have the same interpretation of the message. A big company must have an effective communication network to makean expeditious processes of work, to improve performance of work and make a good working relationship between employees.
In this report, the author is discussing about the process of communication in PT Inti Selera Asia, and the author was as a cashier. The cashier is an information
center whichsend all the messages to other divisions or to the customers.
The communication process is the implementation of the transfering information (message) from one person to another. In PT Asia Inti Selera, a communication process has been very good. Considering the owner have some facilitates the communication media that used in the company. Control of communication are always monitored, and there is always an evaluation like a regular meeting
Co/Ni/Pt(111)的磁性研究
No full text[[abstract]]Experiments were performed in situ in a stainless ultrahigh vacuum (UHV) chamber. In situ magneto-optical Kerr effect (MOKE) was used to study the magnetic properties of the
system.
I study the magnetic properties of ultrathin Co films on Pt(111) with Ni buffer layers. We prepare dCo Co/1 ML Ni/Pt(111), and the Kerr signals measured at room temperature. The polar and longitudinal Kerr signal was not exist for 0 ~ 3 ML Co/1 ML Ni/Pt(111). The easy axis of the magnetization for 4&5 ML Co/ Pt(111) is in-plane. We find the same result, when dCo Co/1 ML Ni/Pt(111) was prepare at 450K, and the Kerr signals measured at 450K or at room temperature. If we prepare dCo Co/1 ML Ni/Pt(111) at RT, and the Kerr signals measured at 450K. The easy axis of the magnetization for 1 ML Co/ Pt(111) is out-of-plane. The polar and longitudinal Kerr signal was not exist for 2&3 ML Co/1 ML Ni/Pt(111). The easy axis of the magnetization for 4&5 ML Co/ Pt(111) is in-plane. The variation of AES of 1 ML Co/1 ML Ni/Pt(111) as a function of sample temperature. We find the mixing of Ni and Co layers occurs at 420K. The formation of Co-Pt alloy causes the perpendicular magnetic anisotropy.
I also study the magnetic properties of ultrathin Ni films on Pt(111) with Co buffer layers. The easy axis of the magnetization for 1 ML Co/ Pt(111) is out of plane. We were surprised that only the polar Kerr signals were observed when dNi 24 ML. Studies of the magnetic properties showed that the easy axis of the magnetization changed from the cant to the out-of-plane direction when the Co buffer layer was inserted on Ni/Pt(111).
After different temperature annealing, cause the different competition alloy. The competition in the alloy formations between Co-Pt and Ni-Pt in the Co/Ni/Pt(111) and Ni/Co/ Pt(111) system is also interesting, cause the different magnetic properties. I also try to understand that.
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