719 research outputs found
PMCA Applications for Prion Detection in Peripheral Tissues of Patients with Variant Creutzfeldt-Jakob Disease
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PMCA Applications for Prion Detection in Peripheral Tissues of Patients with Variant Creutzfeldt-Jakob Disease
by Giorgio Giaccone
and Fabio Moda
* [ORCID]
Fondazione IRCCS Istituto Neurologico Carlo Besta, Division of Neurology 5—Neuropathology, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(3), 405; https://doi.org/10.3390/biom10030405
Submission received: 13 February 2020 / Revised: 2 March 2020 / Accepted: 5 March 2020 / Published: 5 March 2020
(This article belongs to the Special Issue Prion Disease Biomarkers: Recent Advances)
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Abstract
Prion diseases are neurodegenerative and invariably fatal conditions that affect humans and animals. In particular, Creutzfeldt-Jakob disease (CJD) and bovine spongiform encephalopathy (BSE) are paradigmatic forms of human and animal prion diseases, respectively. Human exposure to BSE through contaminated food caused the appearance of the new variant form of CJD (vCJD). These diseases are caused by an abnormal prion protein named PrPSc (or prion), which accumulates in the brain and leads to the onset of the disease. Their definite diagnosis can be formulated only at post-mortem after biochemical and neuropathological identification of PrPSc. Thanks to the advent of an innovative technique named protein misfolding cyclic amplification (PMCA), traces of PrPSc, undetectable with the standard diagnostic techniques, were found in peripheral tissues of patients with vCJD, even at preclinical stages. The technology is currently being used in specialized laboratories and can be exploited for helping physicians in formulating an early and definite diagnosis of vCJD using peripheral tissues. However, this assay is currently unable to detect prions associated with the sporadic CJD (sCJD) forms, which are more frequent than vCJD. This review will focus on the most recent advances and applications of PMCA in the field of vCJD and other human prion disease diagnosis
Pengaruh Metode Ekstraksi Dan Media Semai Terhadap Viabilitas Benih Jabon Merah (Anthocepalus Macrophyllus)
No full textPenelitian ini bertujuan untuk mengetahui pengaruh Metode Ekstraksi dan Media Semai yang tepat terhadap viabilitas benih Jabon Merah (Anthocepalus macrophyllus), dan mengetahui pengaruh interaksi dari metode ekstraksi dengan media semai. Penelitian ini menggunakan Rancangan Acak Lengkap Pola Faktorial dengan dua faktor yaitu metode ekstraksi (A) dan media semai (B). factor Metode Ekstraksi terdiri dari taraf perlakuan yaitu metode ekstraksi basah (A1), metode ekstraksi kering (A2) dan faktor media semai terdiri dari taraf perlakuan yaitu media semai pasir (B1), media semai tanah (B2) dan media semai cocopeat (B3). Dengan tiga ulangan pada masing-masing taraf perlakuan. Penelitian ini menunjukan bahwa pengaruh metode ekstraksi yang paling baik adalah metode ekstraksi basah (A1) dan media semai yang paling baik adalah media semai tanah (B2). Interaksi dari metode ekstraksi dan media semai yang paling baik dan cocok adalah metode ekstraksi basah dan media semai tanah (A1B2), karena dapat meningkatkan viabilitas dari benih Jabon Merah (Anthocepalus macrophyllus)
Moda Fluida e Harry Styles: expressão e transgressão na moda
No full textThis final project aims to present a fashion brand called Volare, created by the author, whose intention is to enter the fluid fashion niche. It also involves the development of a capsule collection with 12 looks inspired by the binary style of British singer Harry Styles, mainly in his distinct stage costumes. Based on bibliographic studies by authors such as Diana Crane (2006), Lipovetsky (2009), Reed, and Young (2022), among others, the binary notion of wearables and some approximations and divergences of genders over the centuries were identified. Finally, a fashion editorial will be produced with the pieces of 3 looks created and developed by the author, proposing to enhance transgression in fashion, freedom of experimentation and identity, and communication through wearables.O presente trabalho de conclusão de curso tem como objetivo a apresentação de uma marca de moda denominada Volare criada pela autora, cuja intenção é a inserção no nicho de moda fluida. Ainda, envolve o desenvolvimento de uma coleção cápsula com 12 looks, inspirada no estilo binário do cantor britânico Harry Styles, principalmente, em seus figurinos de palco distintos. Com base em estudos bibliográficos de autores como Diana Crane (2006), Lipovetsky (2009), Reed e Young (2022), entre outros, foram identificadas a noção binária dos vestíveis e algumas aproximações e divergências dos gêneros ao longo dos séculos. Por fim, será realizado um editorial de moda com as peças de 3 looks criados e desenvolvidos pela autora, propondo potencializar a transgressão na moda, liberdade de experimentação e identidade, e comunicação através dos vestíveis
Pengaruh Jenis Tanah terhadap Semai Mahoni Daun Besar (Swietenia Macrophylla)
No full textBig Leaf Mahogany (Swietenia macrophylla) is a tropical plant commonly found growing wild in teak forests and areas near the coast. This plant is also known for its resilience, meaning it can withstand harsh and arid soil conditions. Even without watering for months, mahogany can still survive. The propagation of this plant can be done through seeds, as well as through air layering or grafting. When used for medicinal purposes, it should not be treated with chemical (inorganic) fertilizers or pesticides. The significance of this research lies in providing valuable information to relevant institutions about the suitable soil type for propagating Big Leaf Mahogany (Swietenia macrophylla) seedlings, ensuring high-quality seedlings for reforestation or afforestation efforts. The research was conducted at the Greenhouse of the Faculty of Agriculture from March to June 2023. The objective of this study was to determine the effect of different soil types on the growth of Big Leaf Mahogany (Swietenia macrophylla) seedlings. The experimental design used in this research was a Completely Randomized Design (CRD) with 2 (two) treatments and 3 (three) replications. The treatments consisted of: A (Regosol soil) and A2 (Podsolik soil). The research results indicate that Regosol soil type provided better average growth results for Big Leaf Mahogany (Swietenia macrophylla) seedlings
La moda en el museo. El caso de la moda argentina
No full textFashion had a more frequent appearance in museums and spaces for the exhibition of art in recent years. In the fashion shows, the culture of the past and present is collected, exhibited and investigated. This essay narrates the interest of museums in fashion and puts the experience of Argentine fashion in context, with references to exhibitions by Fridl Loos, Dalila Puzzovio, Mary Tapia, Gino Bogani, the under movement, the generation of author design and the case of Malba Moda in order to explore the potential of fashion in museums to reflect on history through the ages and its creators.La moda tuvo una aparición más frecuente en los museos y espacios destinados a la exhibición de arte en los últimos años. En las muestras de moda se recopila, exhibe e investiga la cultura del pasado y del presente. Este ensayo narra el interés de los museos por la moda y pone en contexto la experiencia de la moda argentina, con referencias a las exhibiciones de Fridl Loos, Dalila Puzzovio, Mary Tapia, Gino Bogani, el movimiento under, la generación de diseño de autor y el caso de Malba Moda a modo de explorar el potencial de la moda en los museos para reflexionar la historia a través de las épocas y sus creadores.A moda teve um aparecimento mais frequente em museus e espaços de exposição de arte nos últimos anos. Nos desfiles, a cultura do passado e do presente é coletada, exibida e investigada. Este ensaio narra o interesse dos museus pela moda e contextualiza a experiência da moda argentina, com referências a exposições de Fridl Loos, Dalila Puzzovio, Mary Tapia, Gino Bogani, o submovimento, a geração do design autor e o caso da Malba Moda como forma de explorar as potencialidades da moda nos museus para refletir sobre a história ao longo dos tempos e seus criadores
EKO-EDUKASI PENANAMAN POHON DAN KEMANFAATANNYA UNTUK EKOSISTEM LINGKUNGAN BAGI SISWA PENDIDIKAN DASAR
No full textThe tree planting program aims to maintain environmental balance and sustainability to create environmental health and balance the present and future water systems. The tree planting eco-education program is an environmental education effort that has become a popular community movement. This Community Service Program (PKM) aims to increase awareness of 9-year primary education students, especially elementary school students. The extension method uses simple teaching aids in short videos and presentations containing children's animated images regarding tree planting eco-education and its benefits for environmental sustainability, especially water management on the small islands of Maluku Province. The PKM solution measures primary education students' knowledge and attitudes regarding tree planting and its benefits in maintaining partner areas' environmental ecosystem and water management (hydro-orological system). The results of the assessment criteria for students' knowledge and attitudes regarding tree planting and its benefits for the environmental ecosystem are included in the medium category. The expected result of the PKM program is that it can educate students at SD Negeri 300 Central Maluku to improve the knowledge and attitudes of students in the middle category who are increasing in partner areas.
Keywords: benefits of tree planting, small island, elementary school students, water management
Abstrak
Program penanaman pohon merupakan upaya menjaga keseimbangan dan kelestarian lingkungan guna mewujudkan kesehatan lingkungan dan menyeimbangkan tata air masa kini dan masa depan. Program eko-edukasi penanaman pohon merupakan sebuah upaya pendidikan lingkungan yang menjadi gerakan komunitas yang populer. Program Pengabdian Kepada Masyarakat (PKM) ini bertujuan untuk meningkatkan penyadartahuan siswa pendidikan dasar 9 tahun terutama siswa Sekolah Dasar. Metode penyuluhan menggunakan alat peraga sederhana berupa video singkat dan presentasi yang berisi gambar animasi anak mengenai eko-edukasi penanaman pohon dan kemanfaatannya bagi kelestarian lingkungan dan khususnya tata air di pulau kecil Provinsi Maluku. Solusi PKM yang ditawarkan adalah mengukur pengetahuan dan sikap siswa pendidikan dasar mengenai penanaman pohon dan kemanfaatannya dalam menjaga ekosistem lingkungan dan tata air (sistem hidro-orologi) wilayah mitra. Hasil penilaian kriteria untuk pengetahuan dan sikap siswa mengenai penanaman pohon dan kemanfaatannya bagi ekosistem lingkungan termasuk dalam kategori sedang. Hasil yang diharapkan dari program PKM adalah program PKM ini dapat mengedukasi siswa SD Negeri 300 Maluku Tengah meningkatkan pengetahuan dan sikap siswa-siswi yang kategori sedang semakin meningkat di wilayah mitra.
Kata Kunci: kemanfaatan penanaman pohon, pulau kecil, siswa sekolah dasar, tata ai
Derecho de la moda: un concepto entre varios conceptos
No full textThis paper seeks to achieve a definition of Fashion Law. For this, the author has chosen to deconstruct the different elements of the term, explaining each one of them. The deconstruction determines what is Law for the author, what is Fashion and the use of connectors. To specify the task, the Fashion industry and the meaning generated by the use of binding prepositions in the case of Fashion Law and Fashion Law are analyzed. Thoughts, definitions and opinions of the legal sector around the world are collected as an additional contribution. The conclusion provided by the explanation comes from the construction of a concept with the chosen elements of deconstruction.En este trabajo se busca lograr una definición del Derecho de la Moda. Para ello el camino elegido es la deconstrucción de los distintos elementos del término explicando cada uno de ellos. Se determina qué es para la autora el Derecho, qué es la Moda y el uso de los conectores. Para concretar el cometido se analiza la industria de la Moda y el significado que genera el uso de preposiciones vinculantes en el caso de Derecho de Moda y Derecho a la Moda. Se recogen pensamientos, definiciones y opiniones del sector jurídico de alrededor del mundo como aporte adicional. La conclusión que provee la definición del Derecho de la Moda proviene de la construcción de un concepto con los elementos elegidos de la deconstrucción
Consumo de Moda: A Relação Pessoa-Objeto
No full textThis is an interview with Prof. Ana Paula Celso de Miranda on the launch of the second edition of the book “Fashion Consumption: a Person-Object Relationship”. The author talks about her motivation for this work and her 20 years of academic trajectory in the field of fashion. The news this edition brings are flagged by Ana Paula in the interview. In addition to the partnership with the stylist Ronaldo Fraga, in this edition the author counts with the contribution of Prof. Olga Pepece and Profº Eduardo Maciel. The author indicates who is meant to read her book, points out some paths in fashion research and talks about the work she has been developing.Esta é uma entrevista com a Profª Ana Paula Celso de Miranda sobre o lançamento da segunda edição do livro “Consumo de Moda: a Relação Pessoa-Objeto”. A autora fala sobre sua motivação para a escrita dessa obra e seus 20 anos de trajetória acadêmica no campo da moda. As novidades que a edição traz são sinalizadas por Ana Paula na entrevista. Além da parceria com o estilista Ronaldo Fraga, nessa edição a autora conta com a contribuição da Profª Olga Pepece e do Profº Eduardo Maciel. A entrevistada indica a quem se destina a leitura de seu livro, aponta caminhos para a pesquisa de moda e fala sobre o trabalho que vem desenvolvendo
¿Puede la semiótica contribuir a la comprensión de la moda?
No full textEn "¿Es la moda un síntoma de la cultura?", Lucia Santaella nos ofrece una obra que explora la profunda intersección entre moda y cultura desde una perspectiva semiótica peirceana. La autora, conocida por sus estudios en semiótica y comunicación, investiga cómo la moda trasciende su papel como prenda de vestir y se convierte en una expresión compleja y multifacética de la sociedad. A través de un apasionante enfoque teórico, Santaella analiza las dinámicas culturales, sociales e históricas que dan forma a la moda contemporánea.Em A Moda é Sintoma da Cultura?, Lucia Santaella nos oferece uma obra que explora a profunda intersecção entre moda e cultura a partir de uma perspectiva da semiótica peirceana. A autora, conhecida por seus estudos em semiótica e comunicação, investiga como a moda transcende seu papel de vestimenta e se torna uma expressão complexa e multifacetada da sociedade. Por meio de uma abordagem teórica de folego, Santaella analisa as dinâmicas culturais, sociais e históricas que moldam a moda na contemporaneidade.In "A Moda é Sintoma da Cultura?", Lucia Santaella offers us a work that explores the profound intersection between fashion and culture from a Peircean semiotic perspective. The author, known for her studies in semiotics and communication, investigates how fashion transcends its role as clothing and becomes a complex and multifaceted expression of society. Through a deep theoretical approach, Santaella analyzes the cultural, social and historical dynamics that shape fashion in contemporary times
Editorial de moda
No full textTrabalho de Projeto apresentado à Escola Superior de Artes Aplicadas do Instituto Politécnico de Castelo Branco para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Design do Vestuário e Têxtil,Aplicação do n.º 3 do art.º 2.º da Política de Depósito de Documentos do IPCB.O projeto desenvolvido teve como objetivo explorar e investigar a problemática do consumo conspícuo de moda na Europa, onde a perceção da empresa e do
consumidor estiveram interligadas, de forma a encontrar possíveis soluções que trouxessem benefícios e consciencializassem ambos. Esta problemática abrangeu várias temáticas e foi contextualizada através das mesmas, seja a nível ambiental, social ou na moda, onde se inseriu. Através desta problemática, a autora pretendeu criar um editorial de moda para abordar as causas, os efeitos e as temáticas associadas ao consumo conspícuo.
Pretendeu-se neste projeto a criação de um editorial de moda que mostrasse a realidade do consumismo conspícuo e da busca dos consumidores pela validação e da pressão para permanecer nas tendências.
A metodologia utilizada teve uma abordagem mista, envolvendo elementos qualitativos.Abstract: The developed project aimed to explore and investigate the issue of conspicuous fashion consumption in Europe, where the perception of the company and the consumer were interconnected, in order to find possible solutions that could bring benefits and raise awareness for both. This issue encompassed various themes and was contextualized through them, whether at an environmental, social, or fashion level, where it was situated. Through this issue, the author intended to create a fashion editorial to address the causes, effects, and themes associated with conspicuous consumption.
This project sought to create a fashion editorial that would depict the reality of conspicuous consumerism, the pursuit of validation by consumers, and the pressure to stay on-trend.
The methodology used had a mixed approach, involving qualitative elements
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