2,909 research outputs found
Significantly decreased hypoxanthine levels in bone marrow plasma of aplastic anemia patients revealed by 800 MHz NMR based metabolomics analysis
No full textBackground: Compared to plasma, the hypoxanthine (HX) concentration is three times higher in the human bone marrow [R1] and its availability is crucial to regulate the de novo purine synthesis in human bone marrow in vivo [R1]. Studies have shown that circulatory HX levels in the plasma increase in patients with solid tumors and leukemia [R2]. Aplastic anemia (AA) is rare disorder of bone marrow failure characterized by hypocellularity and peripheral cytopenias. As purine biosynthesis is essential for normal cell growth and proliferation, we hypothesized that bone marrow HX levels will be decreased in aplastic anemia (AA) patients and may reset back partially in AA patients showing clinical improvement.
Aim: To compare the HX levels in bone marrow plasma samples of AA patients and control subjects and further to check its metabolic reprogramming in AA patients showing clinical improvement.
Methods: The HX levels of bone marrow plasma metabolites were estimated for 56 AA patients and 20 control subjects using 800 MHz NMR spectroscopy. The NMR spectra were analyzed using NMR suite of commercial software (CHENOMX). The levels of altered metabolites in AA patients w.r.t control were estimated and the values are reported as Mean ± SD (SD is standard deviation). The diagnostic potential is evaluated employing standard receiver operating characteristic (ROC) curve analysis.
Results: The present study aims to compare the bone marrow plasma metabolic profiles of hypoxanthine between age and sex matched AA patients (N=40; mean age 32.31±16.03, male/female ratio of 22/18) and follow-up AA patients (N=16) with respect to age matched control subjects (N=20, mean age=39.5±18.48 years, male/female ratio of 11/9). The comparison revealed that the HX levels are significantly decreased in AA patients (HX=3.62 ± 3.17) compared to NC subjects (HX = 20.89 ± 12.73) and improved slightly in follow-up patients (HX=6.21 ±4.77, however no statistically significant change compared to AA patients). Further, the circulatory HX levels exhibit significant diagnostic potential with area under ROC (AUROC) curve values equal to 0.96 [95%CI = 0.92–1.00].
Concluding remarks: The depleted HX levels in the bone marrow plasma suggested altered/compromised purine biosynthesis/metabolism in AA patients and future studies are warranted to underscore its role in the disease pathobiology.
Reference:
[R1] King, M.E., Honeysett, J.M. and Howell, S.B., 1983. Regulation of de novo purine synthesis in human bone marrow mononuclear cells by hypoxanthine. The Journal of clinical investigation, 72(3), pp.965-970.
[R2] Wung WE, Howell SB. Hypoxanthine concentrations in normal subjects and patients with solid tumors and leukemia. Cancer research. 1984 Jul;44(7):3144-8.Compared to control subjects, the multivariate statistical analysis revealed that there are significantly decreased levels of hypoxanthine, citrate, lactate and glutamine in the bone marrow plasma of AA patients. On the other hand, the circulatory levels of acetate and pyruvate were significantly increased in the bone marrow plasma samples of AA patients compared to control subjects.
The investigation is an extension of our previous study
Srivastava, Jyotika, Shobhita Katiyar, Chandra P. Chaturvedi, and Soniya Nityanand. "Extracellular vesicles from bone marrow mesenchymal stromal cells of severe aplastic anemia patients attenuate hematopoietic functions of CD34+ hematopoietic stem and progenitor cells." Cell Biology International 46, no. 11 (2022): 1970-1976
Data for Gupta et al., "Estimating the Meridional Extent of Adiabatic Mixing in the Stratosphere using Age-of-Air", JGR:Atmospheres,
No full textModel data and post-processed data supporting the creation of the manuscript "Estimating the Meridional Extent of Adiabatic Mixing in the Stratosphere using Age-of-Air" submitted to JGR:Atmospheres in August 2022.
1) The netCDF files created through post-processing of full model data in FORTRAN are shared in the /data/ directory. These file contains the zonal mean circulation statistics based on Gupta et al. (2020), age-of-air transport diagnostics based on Linz et al. (2021), and the novel \Gamma-\Theta circulation streamfunction introduced in this study. The /data/ directory also contains MATLAB .mat data files for the transport diagnostics obtained from WACCM. 150 days of actual GFDL-FV3 model data in the northern hemisphere, between 0.1 hPa-500 hPa pressure levels is also provided to support external computations and validation.
2) The Jupyter notebook used for final computation and figures production is provided in .ipynb, .html and .pdf formats in /code/. All the files referred to in the notebook are stored in the /data/ directory.
Corresponding author : Aman Gupta, [email protected], [email protected], [email protected]
Corrigendum: Capital Inflows and House Prices: Aggregate and Regional Evidence from China
No full textIn the paper ‘Capital Inflows and House Prices: Aggregate and Regional Evidence from China’ by H. An, et al., printed in the December 2016 issue, there was a missing acknowledgement section for funding resources.
On page 451, the acknowledgement section should appear after the corresponding information as:
“Correspondence: Rakesh Gupta, Department of Accounting, Finance and Economics, Griffith Business School, Griffith University, Nathan Campus QLD 4111. [email protected]
*This work was financially supported by the Humanities and Social Science Foundation of Ministry of Education of China (16YJA790001).”
The author apologises for this error and any confusion it may have caused.No Full Tex
First person – Akash Gupta
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Biology Open, helping early-career researchers promote themselves alongside their papers. Akash Gupta is first author on ‘A novel and cost-effective ex vivo orthotopic model for the study of human breast cancer in mouse mammary gland organ culture’, published in BiO. Akash conducted the research described in this article while a PhD Scholar in Rajendra Mehta's lab at IIT Research Institute, Chicago, USA. He is now an assistant research scientist in the lab of Syreeta L. Tilghman at the University of Arizona, Department of Medicine, Tucson, USA, investigating drug efficacy modeling using human organoids culture for the treatment of cancers
Engineering materials : research, applications and advances / author, K.M. Gupta.
No full text"A CRC title."Includes bibliographical references and index.596 p.
Universal Statistical Properties of Inertial-particle Trajectories in Three-dimensional, Homogeneous, Isotropic, Fluid Turbulence
No full textWe obtain new universal statistical properties of heavy-particle trajectories in three-dimensional, statistically steady, homogeneous, and isotropic turbulent flows by direct numerical simulations. We show that the probability distribution functions (PDFs) P(Φ), of the angle Φ between the Eulerian velocity u and the particle velocity v, at a point and time, scales as P(Φ) ∼Φ−, with a new universal exponent ≃ 4
An application of the ABS LX algorithm to multiple sequence alignement
No full textIl problema del multiple sequence alignement porta alla soluzione di sistemi lineari malcondizionati, per il cui trattamento si è usato con esiti positivi il metodo implicito LOX della classe ABSWe present an application of ABS algorithms for multiple sequence alignment (MSA). The Markov decision process (MDP) based model leads to a linear programming problem (LPP), whose solution is linked to a suggested alignment. The important features of our work include the facility of alignment of multiple sequences simultaneously and no limit for the length of the sequences. Our goal here is to avoid the excessive computing time, needed by dynamic programming based algorithms for alignment of a large number of sequences. In an attempt to demonstrate the integration of the ABS approach with complex mathematical frameworks, we apply the ABS implicit LX algorithm to elucidate the LPP, constructed with the assistance of MDP. The MDP applied for MSA is a pragmatic approach and entails a scope for future work. Programming is done in the MATLAB environment
Serum albumin predicts survival in Indian adult diffuse large B cell lymphoma patients in the rituximab era
No full textObjective: The present study was done to evaluate the prognostic impact of the National Comprehensive Cancer Network-International Prognostic Index (NCCN-IPI) and serum albumin levels in the treatment outcome of Indian diffuse large B-cell lymphoma (DLBCL) patients in the rituximab era. Patients and Methods: We retrospectively analyzed the data (2013–2016) of 135 newly diagnosed DLBCL cases ≥18 years of age. All patients received Rituximab-Cyclophosphamide, Adriamycin, Vincristine, Prednisone (R-CHOP) chemotherapy. The analysis was carried out to assess the overall survival (OS) and progression-free survival (PFS) and the prognostic factors predicting the outcome. Results: Of the 135 patients in the study, 89 (65.9%) had B-symptoms, 20 (14.8%) had bulky disease, 79 (58.5%) had advanced disease (Stage III and IV), and 29 (21.5%) had primary extranodal involvement. Serum albumin ≤3.5 g% was present in 71 (52.6%) patients. About 74 (54.8%) cases were risk stratified to NCCN-IPI high-intermediate-risk group, while 18 (13.3%) patients were categorized into high-risk group. The median PFS and OS of our study cohort were 19 months (95% confidence interval [CI] = 2.59–35.4) and 38 months (95% CI = 9.02–55.68), respectively. Serum albumin ≤3.5 g/dl was significantly associated with poor OS (hazard ratio [HR] = 3.99, 95% CI = 2.25–7.07, P < 0.001) and PFS (HR = 3.71, 95% CI = 2.20–6.26, P < 0.002). Similarly, low NCCN-IPI (<4) was significantly associated with improved OS (HR = 0.21, 95% CI = 0.09–0.47, P < 0.005) and PFS (HR = 0.19, 95% CI = 0.09–0.41, P < 0.001), respectively. These two factors (serum albumin and NCCN-IPI) retained their prognostic significance with respect to OS and PFS in the multivariate analysis. Conclusion: The NCCN-IPI prognostic model and serum albumin levels have independent prognostic significance in Indian DLBCL patients. Serum albumin is a readily available, easy to standardize, and cheap investigation requiring no specialized expertise and holds promise for being incorporated in future DLBCL prognostic risk models
Mutual-Friction Coefficients in Two-Dimensional Superfluids: From the Gross-Pitaevskii equation to the Hall-Vinen-Bekharevich-Khalatnikov Two-fluid Model
No full textWe start from the two-dimensional Gross-Pitaevskii equation (GPE) and develop algorithms for the ab-initio determination of the temperature (T) dependence of the mutual-friction coefficients, α and α, and the normal-fluid density Pn, which appear as parameters in the Hall-Vinen-Bekharevich-Khalatnikov (HVBK) two-fluid model for a superfluid. In the second part of our study, we elucidate the statistical properties of two-dimensional, homogeneous, isotropic superfluid turbulence in the simplified HVBK model, with values for the mutual-friction coefficients that are comparable to those we obtain from the first part of our study
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