161,300 research outputs found
Transforming growth factor-alpha stimulates proliferation of rat sertoli cells
No full textThe number of Sertoli cells is positively correlated with the number of germ cells produced in the testis, but the regulation of Sertoli cell proliferation and final density is poorly understood. Using non-aggregated Sertoli cells from 8 to 9-day-old rat testes, highly enriched by lectin binding, we explored effects of Sertoli cell growth factor candidates in vitro. Proliferation was assessed by H-3-thymidine incorporation, bromodeoxyuridine labeling and supravital staining, and FSH was used as positive control. Transforming growth Factor-alpha (TGF-alpha) was found to stimulate Sertoli cell proliferation in a dose-dependent manner. Epidermal growth factor (EGF) and betacellulin mimicked the effect, demonstrating specificity of the response as they share receptors with TGF-alpha. Insulin-like growth factor I and II, acidic and basic fibroblast growth factor and stem cell factor lacked significant stimulatory effects. We conclude that EGF/TGF-alpha is a growth factor for Sertoli cells in vitro, possibly contributing to paracrine regulation of Sertoli cell proliferation in vivo. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved
Interleukin-1 is a potent paracrine growth and differentiation factor for immature rat Sertoli cells
No full textVariations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Aplicabilidade dos frameworks de implementa??o da gest?o do conhecimento : estudo de caso em empresas do setor cal?adista
No full textMade available in DSpace on 2015-04-14T14:52:05Z (GMT). No. of bitstreams: 1
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Previous issue date: 2009-08-18Esta ? uma pesquisa qualitativa, sendo um estudo de caso m?ltiplo, onde se realizou an?lise de documentos e entrevistas em profundidade, com corte transversal. Ela tem como base o estudo de seis frameworks de implementa??o da gest?o do conhecimento, que s?o ferramentas que direcionam as empresas a encontrarem o melhor caminho para a solu??o dos seus problemas. Essa pesquisa foi aplicada em tr?s empresas do setor cal?adista. Como resultados a que a pesquisa chegou, pode-se dizer: o n?mero de categorias com mais dificuldades apresentadas pelas empresas de grande porte foi menor, j? o n?mero de categorias com mais dificuldades apresentadas pela empresa de m?dio porte foi maior; as empresas de grande porte apresentaram mais facilidades em pontos relevantes, como estrat?gia, suporte da lideran?a, sistemas de informa??o, comunica??o e estrutura; as empresas de grande porte apresentaram ambas tr?s categorias com mais dificuldades, sendo que a empresa A apresentou mais dificuldades em capital humano, sistema de recompensas e treinamento, enquanto o caso piloto apresentou mais dificuldades em capital humano, sistema de recompensas e cultura. Ao se comparar o quadro final com o quadro dos frameworks que serviram de base para essa pesquisa, percebe-se que as empresas A, B e caso piloto n?o se encaixam em nenhum framework, o que permite concluir que nenhuma das empresas tem de fato a gest?o do conhecimento implementada em suas empresas. Isto porque as empresas capacitadas, que seriam a empresa A e o caso piloto, ambas tiveram o n?mero de entrevistados que apresentou facilidades igual ao n?mero de entrevistados que apresentou dificuldades no t?pico processo, que aparece em todos os frameworks, o que n?o permite concluir se as empresas apresentam mais facilidade ou mais dificuldade em rela??o a esse t?pico
Promovendo à saúde ao atleta de voleibol: a multidimensionalidade na gestão do cuidado de saúde e enfermagem
No full textTese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências da Saúde, Programa de Pós-Graduação em Enfermagem, Florianópolis, 2013.A presente tese de doutorado está inserida na linha de pesquisa Políticas, Gestão e Avaliação do Cuidado em Saúde e Enfermagem do Grupo de Estudos e Pesquisas em Administração, Gerência do Cuidado e Gestão Educacional em Enfermagem e Saúde - GEPADES. Trata-se de um estudo envolvendo a gestão do cuidado a saúde e enfermagem do atleta de voleibol de alto rendimento. O ambiente esportivo, em especial o voleibol, mostrou-se como um terreno fértil para a realização de um estudo que abrangesse a saúde do jogador de voleibol, visto que esse espaço é carregado de especificidades e peculiaridades no seu enredo, sendo transversalizado por inúmeros condicionantes e determinantes que perpassam o contexto social e da saúde do atleta. A enfermagem é uma profissão que permea diferentes campos de atuação do ciclo do viver humano, que promove a saúde das pessoas e com isso amplia os horizontes de seus profissionais. Assim, as questões de pesquisa foram: Como o atleta de voleibol de alto rendimento vem experienciando o cuidado a saúde? E, quais significados atribuem a essa vivência na perspectiva da complexidade? O objetivo central do estudo foi de construir uma matriz de conhecimentos substantivos sobre o fenômeno que abrange a gestão do cuidado de saúde e enfermagem do atleta de voleibol de alto rendimento na perspectiva do pensamento complexo, a partir da compreensão dos significados atribuídos pelos referidos atletas sobre o cuidado a sua saúde. Para tal ancorou-se no referencial teórico do paradigma da complexidade, no percurso metodológico da Grounded Theory ou Teoria Fundamentada nos Dados (TFD), a qual considera que as pessoas agem de acordo com o propósito interativo, seguindo seu próprio entendimento dentro de uma situação. Assim, considerou-se o cenário de relações, interações e associações peculiares do viver do12atleta, e a amostra teórica constituiu-se de 34 participantes entrevistados (atletas, ex-atletas, técnicos e dirigentes), distribuídos em 3 grupos amostrais, tendo como período de realização da coleta dos dados de fevereiro a setembro de 2013. Os resultados obtidos estão apresentados na forma de 4 manuscritos, assim organizados: no primeiro manuscrito, foi realizado uma profunda e refinada busca nas bases PUBMED, CINAHL, LILACS e SciELO num intervalo de tempo de 7 anos (janeiro de 2007 a agosto de 2013), quando pode-se certificar que a literatura envolvendo voleibol, cuidado e enfermagem é incipiente, praticamente inexistente, configurando um excelente campo para inserção da enfermagem; o segundo manuscrito, aponta que a gestão do voleibol transita entre o profissionalismo e o amadorismo, apresentando-se frágil, carente em estrutura e organização, comprometendo assim a organização de um sistema de gestão de cuidado a saúde do atleta; o terceiro manuscrito, conduz a uma viagem pelos significados atribuídos pelos atletas a saúde, ao cuidado e a doença, constituindo o trinômio saúde-cuidado-doença no voleibol, ficou evidenciado que os jogadores possuem uma visão segmentada sobre o trinômio, explicitando o entendimento de que o corpo é considerado apenas o instrumento de trabalho e não o instrumento do viver; no quarto e último manuscrito, descreve a configuração da tese de forma geral, perpassa pela origem e confecção das 4 categorias emergidas no estudo e suas inter-relações com as subcategorias, até chegar na configuração do fenômenos central ?Gestão do cuidado no contexto do jogador de voleibol de alto rendimento: (sobre)vivendo na multidimensionalidade do ambiente esportivo?. A partir de uma reflexão crítica sobre o conjunto dos manuscritos, evidencia-se que a gestão do cuidado a saúde e enfermagem no voleibol é um campo muito denso e intenso, porém ainda um pouco inóspito, que carece de maior atenção e valorização pela importância que representa no cenário esportivo. No conjunto dos resultados finais dessa tese, pode-se afirmar que "A gestão do cuidado a saúde e enfermagem ao atleta de voleibol de alto rendimento é complexa, dinâmica, pró-ativa, interdependente e perpassa por dessemelhantes interações entre atores sociais envolvidos no contexto esportivo", permitindo assim, a organização de um sistema de gestão de cuidado a saúde e enfermagem que contemple a totalidade do atleta de voleibol de alto rendimento.Abstract : The present doctorate's thesis is inserted in the line of research Politics, Management and Healthcare Evaluation in Health and Nursing in the Study and Research Group in Administration, Care Management and Educational Management in Nursing and Health - GEPADES (acronym in Portuguese). This is a study involving healthcare and nursing management in high performance volleyball athletes. The sports environment, especially the volleyball, showed itself a productive area in order to carry out a study which would cover the volleyball players? health, once this space is full of specificities and peculiarities in its plot, being involved by several conditioning and determining factors which are part of the social and health player's context. Nursing is a profession which permeates different playing fields in the cycle of human living, which promotes people's health and this way it amplifies their professionals' horizons. Thus, the questions in the research were: How has the high performance volleyball athlete been experiencing healthcare? And, which meanings do they attribute to this living in the perspective of complexity? The main goal in the study was to build a matrix of substantive knowledge about the phenomenon which covers healthcare and nursing management of a high performance athlete in a complex thought perspective, from the understanding on the attributed meanings by the mentioned athletes about the care to their health. In order to do that, the theoretical approach was based on the complexity paradigm, in the methods of Grounded Theory, which considers people to act according to an interactive purpose, following their own understanding inside a situation. This way, the scenario of relationship, interactions and peculiar associations of the athlete's life, and the theoretical sample is consisted of 35 participants who were interviewed (athletes, ex-athletes, coaches and the board of directors), distributed in 3 sample groups, while the period of data collection was from February to September, 2013. The results are presented in the form of 4 manuscripts organized in the following way: in the first manuscript, a14deep and careful search was carried out on the databases PUBMED, CINAHL, LILACS and SciELO in a time interval of 7 years (January, 2007 to August, 2013), when it is possible to certify that the literature involving volleyball, care and nursing is incipient, almost inexistent, making an excellent field for inserting nursing; the second manuscript tells that the volleyball management walks between professionalism and amateurism, showing itself fragile, needy in structure and organization, compromising the organization of a management system of the player's healthcare, the third manuscript leads to a trip through the meaning attributed by the athletes to health, care and disease, constituting the trinomial health-care-disease in volleyball, it was evident that the players have a segmented view about the trinomial, clarifying the understanding that the body is considered only a tool to work and not a living tool; the fourth and last manuscript describes the setting of the thesis in a general way, going through the origin and production of 4 categories emerged in the study and its interrelations with the subcategories, till it gets to the setting of the central phenomena "Caring management in the context of high performance volleyball player: surviving/living in the multidimensionality of sports environment". From a critical reflection about the set of manuscripts, it is possible to stress that the management of healthcare and nursing in volleyball is a very dense and intense field, however, still a little inhospitable which needs more attention and value due to the importance it represents in the sports scenario. In the set of the final results of this thesis, it is possible to affirm that "The healthcare and nursing management to the high performance athlete is complex, dynamic, pro-active, interdependent and has different interactions between social actors involved in the sports context", allowing this way, the organization of a management system of healthcare and nursing which contemplates the whole high performance volleyball athlete
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Larry O. Spencer, Conference Author Presentation
No full textGen. Larry O. Spencer, USAF (Ret.), author of Dark Horse: A Journey from the Horseshoe to the Pentago
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Phytoestrogen resveratrol suppresses steroidogenesis by rat adrenocortical cells by inhibiting cytochrome P450 c21-hydroxylase
No full textBackground and Aim: The phytoestrogen resveratrol is found in grapes, mulberries and peanuts, all of which are consumed regularly by humans. Resveratrol is also used in chemotherapy against cancer and aging and as a cardioprotectant. The aim of the present study was to characterize the effects of resveratrol on rat adrenal steroidogenesis and to study the underlying mechanism. Methods: Adrenocortical cells were isolated from the adrenal glands of normal male rats ( in vitro) and from male rats administered resveratrol in their diet for 12 weeks ( ex vivo). Cells from resveratrol-treated and non-treated rats were tested ex vivo for responsiveness to ACTH and cells from normal rats were tested in vitro for responsiveness to ACTH in the presence and absence of resveratrol. Corticosterone and progesterone production were measured by RIA and expression of steroidogenic enzymes analyzed by PAGE/Western blotting. Results: Corticosterone production was inhibited 47% by 50 mu M resveratrol in vitro and 20% ex vivo, while progesterone production was elevated to 400% of the control value in in vitro experiments. Resveratrol treatment decreased adrenal cytochrome P450 c21-hydroxylase expression in vivo and cell culture conditions. No changes in cell viability or morphology were caused by exposure to resveratrol in both ex vivo and in vitro experiments. Conclusion: Resveratrol suppresses corticosterone production by primary rat adrenocortical cell cultures in vitro and ex vivo by inhibiting cytochrome P450 c21-hydroxylase. Copyright (c) 2005 S. Karger AG, Basel
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