7,508 research outputs found
Transformacion(es) del trabajo: Tiempo(s) de precariedad(es) y resistencia(s)”. Algunas aproximaciones desde Latinoamérica
No full textReseña del libro “Transformacion(es) del trabajo: Tiempo(s) de precariedad(es) y resistencia(s)”. Algunas aproximaciones desde Latinoamérica”Rodríguez LL., María Angélica; Vidal M., Paula (2003)Escuela de Trabajo Social Universidad Católica Silva Henríquez y Espacio Editorial, Buenos Aires, Argentina.</jats:p
Transformacion(es) del trabajo: Tiempo(s) de precariedad(es) y resistencia(s)". Algunas aproximaciones desde Latinoamérica
No full textReseña del libro "Transformacion(es) del trabajo: Tiempo(s) de precariedad(es) y resistencia(s)". Algunas aproximaciones desde Latinoamérica"Rodríguez LL., María Angélica; Vidal M., Paula (2003)Escuela de Trabajo Social Universidad Católica Silva Henríquez y Espacio Editorial, Buenos Aires, Argentina
Sustainable agriculture – Poland and Portugal
No full textPapers from communications presented at the Workshop “Agriculture
Sustainability, Poland-Portugal”, held in the University of Évora,
Portugal, December, 2013
Estudo da viabilidade técnica da determinação do limite de liquidez dos solos finos pela mesa de consistência
No full textMonografiaA determinação do Limite de Liquidez dos solos (LL) pelo aparelho de Casagrande é prática corriqueira dos laboratórios de solos. Tal aparato, proposto há mais de 60 anos, apresenta erros decorrentes da influência do operador sobre a realização do ensaio. Com vistas a contribuir para a redução destes erros experimentais, este trabalho avalia, de forma inovadora, a metodologia de determinação da consistência das argamassas pela mesa de consistência (Flow Table) como alternativa ao aparelho de Casagrande para determinação do LL de solos finos. Para tanto, estuda-se aqui o comportamento de 04 solos finos obtidos no município de Mossoró/RN, ensaiadas na mesa de consistência e analisadas segundo metodologia proposta por Hyun-Ki, Cortes e Santamarina, (2007) e Wang e Hu (2005) que descrevem algebricamente tal fenômeno. Considerando a equação de Hyun-Ki, Cortes e Santamarina (2007) e considerando que a resistência ao cisalhamento não drenada dos solos no LL tem um valor aproximadamente igual a 2,5kPa, Bicalho et al. (2017), calculou-se o LL pelo método da mesa de consistência. Os resultados obtidos para os 04 solos ensaiados foram satisfatórios, segundo critério de Suits et al. (2007), apresentando erro inferior a 10% quando comparado com valores experimentais obtidos pelo método tradicional de Casagrande. Concluiu-se que há viabilidade na determinação do LL pela mesa de consistência. Dada a natureza inovadora da análise de solos pela mesa de consistência, sua implementação carece de estudos que corroborem os resultados aqui encontradosTrabalho não financiado por agência de fomento, ou autofinanciad
Enna rioja Silva, 2013, new species
No full textEnna rioja new species Figs 1–7 Type material: Holotype: female from San Martin, Rioja, Paitoja, Peru [06°03’S, 77 ° 10 ’W], 10.X. 2008, C. Albujar (MUSM 505344). Paratype: one female, same locality, date and collector as holotype, deposited at FMNH. Etymology. The specific name is a noun in apposition taken from type locality. Diagnosis. The female of E. rioja sp. nov. resembles those of E. baeza (Silva et al., 2008: 93, figs 78, 79) by the small rounded projection on the head of spermathecae (HS) and E. osaensis (Silva et al., 2012: 57, fig. 6) by the presence of a large accessory spermathecae (AS) and by the shape of the projected scape (SC) of the middle field of the epigynum (MF), but can be distinguished from both species by the slightly projected lateral lobes (LL) (Fig. 5) and by the large and conspicuous accessory spermathecae and by the elliptical shape of head of spermathecae (HS) (Fig. 6). Note. Ten species of Enna were described from Peru and Enna huanuco Silva, Lise & Carico, 2008 is the only one with the female still unknown. In this paper, the female from San Martin, Peru is described as separate species and not grouped with E. huanuco, since the locations where they have been found are not close together (San Martin and Huanuco, respectively), distant about 400km apart and males and females have not been collected together from the same area. Both species (E. rioja sp. nov. and E. huanuco Silva, Lise & Carico, 2008) also present some morphological differences: carapace colouration (E. huanuco is dark brown with lateral brownish bands and E. rioja sp. nov. has a dark brown carapace without bands) and carapace dimensions (most Enna males have a longer and wider carapace than females, but the female of E. rioja sp. nov. has a longer and wider carapace compared to the male specimen of E. huanuco). Description. Female (Holotype, San Martin, Peru, MUSM 505344). Total length 10.50. Carapace 5.58 long, 4.78 wide, dark brown, fovea marked (Figs 1, 2). Clypeus dark brown, 0.46 high (Fig. 4). Anterior eye row straight, 1.51 wide; posterior 2.35 wide (Fig. 4). Eye diameters, interdistances, and median ocular quadrangle: AME 0.21, ALE 0.18, PME 0.31, PLE 0.18; AME-AME 0.18, AME-ALE 0.16, PME-PME 0.65, PME-PLE 0.49, OQA 0.65, OQP 1.20, OQH 0.71. Chelicerae reddish-brown, bristly (Fig. 4); promargin and retromargin of fang furrow with three teeth equidistant and equal in size. Sternum yellow, with scattered setae (Fig. 3); 2.66 long, 2.52 wide. Labium dark brown, yellow distally (Fig. 3), 1.06 long, 0.93 wide. Legs light brown with dark brown annuli on femora (Figs 1, 2), relative length: IV-I-II-III, I—femur 6.25 / tibia-patella 8.64 / metatarsus 6.65 / tarsus 2.66 / total 24.20; II— 6.38 / 8.37 / 6.11 / 2.55 / 23.41; III— 5.18 / 5.45 / 4.12 / 2.12 / 16.87; IV— 6.65 / 7.98 / 7.31 / 2.92 / 24.85. Ventral pairs of macrosetae on tibiae: I– 4; II– 3; III– 3; IV– 3. Abdomen 4.92 long, gray with two pairs of sigillae (Fig. 1). Venter light brown, bristly (Fig. 3). Epigynum with a projected scape (SC) (Fig. 5). Head of spermathecae (HS) rounded and with a rounded whitish projection (Fig. 6, HS). Accessory spermathecae conspicuous and rounded (Fig. 6, AS). Distribution. Known only from the type locality (Peru) (Fig. 7). Acknowledgments I wish to thank Diana Silva-Dávila (MUSM) for the loan of the material. The images were taken in Petra Sierwald’s lab at the Field Museum, Robin Delapena (FMNH) graciously helped obtaining the photos of the female genitalia.Published as part of Cruz Da Silva, Estevam L., 2013, A new Peruvian species of Enna (Araneae, Lycosoidea, Trechaleidae), pp. 467-470 in Zootaxa 3701 (4) on pages 467-470, DOI: 10.11646/zootaxa.3701.4.6, http://zenodo.org/record/21940
La Silva 5.4 de Estacio: Plegaria al sueño
No full textEl objeto de este artículo es ofrecer una nueva interpretación literaria de la Silva de Estacio al dios Sueño (silu V. 4). Del examen minucioso de los principales motivos de la Silva y de su tradición literaria se derivan dos conclusiones principales: 1) el poeta en su dolor se identifica poéticamente con Orfeo, según sugiere el motivo de la conmiseración de la Naturaleza (vv. 3b-6) y el de los astros contemplando su llanto (vv. 7-10); 2) el dios Somnus que Estacio invoca asume atributos propios de Mercurio y el poeta solicita de él no tanto un sueño profundo como una muerte tranquila. Ambas conclusiones sugieren que la causa del insomnio del poeta sea el dolor por la muerte de un ser querido, probablemente su mujer. Desde un punto de vista genérico la Silva manipula tres tradiciones: la himno crético, la del epicedio y la de la poesía erótica.The purpose of this paper is to offer a new literary reading of Statius Silvae V 4 (Somnus), on the basis of a detailed survey of the main motifs of the poem and their literary tradition. Two main points are concluded: 1) the poet identifies himself with Orpheus, as is suggested by the theme of Nature’s sympathy for him (ll. 3b-6) and by the scene of the heavenly bodies witnessing his grief (ll. 7-10); 2) the god Somnus to whom Statius prays is virtually identified with Mercurius: Statius asks him not for a deep sleep but for a sweet death (ll. 16-19). Both conclusions strongly suggest that the biographical cause of Statius’ sleeplessness is his grief for the loss of a close acquaintance, most likely his wife. From a generic point of view, the Silva takes up three different literary traditions: that of the kletic hymn, the epicedion and the erotic poetry (specially elegy)
Antimicrobial peptide LL-37 and its effects on stemness in different cancer cells
No full textOs peptídeos antimicrobianos desempenham papéis protetores críticos em uma gama de doenças humanas, incluindo o câncer. Vários estudos demonstraram funções - tais como proliferação, angiogênese, apoptose e imunomodulação - desses peptídeos em vias cancerígenas cruciais. Investigamos o papel do Peptídeo antimicrobiano LL-37 sobre stemness em câncer de mama (SKBR3) e células de melanoma (A375). Análise por PCR array da expressão diferencial de genes em SKBR3 e A375 com knockdown por siRNA para o mRNA de LL-37 revelou uma regulação negativa de genes relacionados com stemness, incluindo transcriptase reversa da telomerase, forkhead box D3 e para o fator indiferenciado de transcrição de células embrionárias 1, notavelmente em células de câncer de mama.Além disso, as células SKBR3 com knockdown para a expressão de LL-37 mostraram uma diminuição da produção de oncosferas em comparação com controles negativos, enquanto as células A375 exibiram uma produção aumentada. Tomados em conjunto, nossos achados indicam um papel para LL- 37 em stemness, dependendo do tipo de celular analisadoAntimicrobial peptides play critical protective roles in a range of human diseases, including cancer. Multiple studies have demonstrated functions -- such as proliferation, angiogenesis, apoptosis and immunomodulation -- of these peptides in crucial cancer pathways. We investigated the role of the antimicrobial peptide LL-37 on stemness in breast cancer (SKBR3) and melanoma cells (A375). PCR array analysis of differential gene expression in SKBR3 and A375 cancer cell lines downregulated for LL-37 expression by siRNA revealed downregulation of genes related to stemness, including telomerase reverse transcriptase, forkhead box D3 and undifferentiated embryonic cell transcription factor 1, remarkably in breast cancer cells. Furthermore, SKBR3 cells knocked down for LL-37 expression showed a decreased production of oncospheres in comparison with negative controls, while A375 cells exhibited increased production. Taken collectively, our findings indicate a role for LL-37 in cancer cell stemness depending on the cell typ
Natalia LL - artystka neoawangardowa
No full textThe paper shows Natalia Lach-Lachowicz (Natalia LL) as a neo avant-garde artist whose works in a specific maximalistic way are very close to the main currents of avant-garde trends: new mediality (photography), minimalism, conceptualism, performance, bodyart, pop-art, and feminist art. The author of the article concentrates mainly on the mutual influences of conceptualism, consumptionism, and feminism in Natalia LL’s works and pays attention to the emancipatory potential of her works of the seventies and the eighties
Paratrechalea julyae Silva & Lise 2006
No full text<i>Paratrechalea julyae</i> Silva & Lise 2006 <p>Figs 4–11</p> <p> <i>Paratrechalea julyae</i> Silva & Lise 2006: 72, figs 1–6 (Male holotype, Fazenda Paissandu, Mucuri,Bahia, Brazil, 15.VI.1979, A. C. Niella <i>leg</i>., deposited in MCN 10851, examined).</p> <p> <b>Diagnosis.</b> The female of <i>P. julyae</i> is similar to <i>P. saopaulo</i> Carico, 2005 (see Carico 2005: fig. 32) and <i>P. wygodzinskyi</i> (Soares & Camargo, 1948) (see Silva <i>et al</i>. 2006: fig. 19) by the projected shape of the scape of the middle field of epigynum, but can be distinguished by the longer and triangular shape of the scape and the excavated lateral lobes (LL) (Figs 4, 6).</p> <p> <b>Description.</b> Male. Described by Silva <i>et al</i>., 2006: 72, figs 1–6. New remarks: male palpus with excavated spur on tip of guide of median apophysis (Figs 7–9) and prominent retrolateral tibial apophysis (Figs 10–11).</p> <p>Female (São Paulo, Parque Estadual da Serra do Mar, Ubatuba, Brazil IBSP 6989). Total length 7.47. Carapace, 2.90 long, 2.73 wide, light brown with two brownish longitudinal bands; dark brown laterally. Clypeus brownish, 0.24 high. Anterior eye row slightly straight, 0.71 wide; posterior 1.33 wide. Eye diameters, interdistances, and median ocular quadrangle: AME 0.13, ALE 0.12, PME 0.18, PLE 0.20; AME- AME 0.09, AME-ALE 0.06, PME-PME 0.24, PME-PLE 0.27, OQA 0.34, OQP 0.72, OQH 0.55. Chelicerae bristly, yellowish, with a median light brown band, without lateral carina; promargin and retromargin of fang furrow with three teeth equidistant and equal in size. Sternum yellowish, light brown laterally, with irregular median spot; 1.41 long, 1.32 wide. Labium light brown, darker posteriorly, 0.33 long, 0.49 wide. Legs brownish with light brown annuli on femora, relative length: I-II-IV-III, I – femur 3.32/ tibia-patella 4.31/ metatarsus 3.15/ tarsus 1.90/ total 12.58; II – 3.23/ 4.15/ 2.98/ 1.66/ 12.02; III – 1.82/ 1.74/ 1.49/ 0.83/ 5.88; IV – 2.57/ 2.65/ 2.90/ 1.16/ 9.28. Ventral pairs of macrosetae on tibiae: I-4; II-3; III-3; IV-3. Abdomen, 3.98 long, bristly, grayish, lighter at anterior portion, with scattered setae. Venter grayish, with scattered setae. Middle field of epigynum with projected scape (Figs 4, 6); excavated lateral lobes (LL) (Figs 4, 6), spermathecae rounded at apex (Fig. 5).</p> <p> <b>Other material examined.</b> BRAZIL, <i>Bahia</i>: Camacan (15°24’S, 39°30’W), 1 ♂, no date, CEPLAC <i>leg.</i> (IBSP 15813); Gandu, Fazenda São Rafael (13°44’S, 39°28’W), 1 ♀, 08.V.1969, CEPLAC <i>leg</i>. (IBSP 15810); <i>São Paulo</i>, Parque Estadual da Serra do Mar, Ubatuba (23°26’S, 45°05’W), 1 ♀, IV.1996, L. S. Rocha <i>leg.</i> (IBSP 6989); <i>Rio de Janeiro</i>: Pinheral, Fazenda Santa Helena (22°31’S, 43°59’W), 1 ♂, 2 ♀, (22° 34’S, 44° 21’W), 05–11.XI.1999, A. D. Brescovit <i>et al</i>. <i>leg</i>. (IBSP 52743; 52737), 3 ♂, 4 ♀ (IBSP 52734), 1 ♂ (IBSP 52736); Marambaia, Ilha de Marambaia (22°49’S, 42°56’W), 1 ♂, 2 ♀, 23–25.XI.2007, M. Silveira <i>leg</i>. (IBSP 88603).</p> <p> <b>Distribution.</b> Brazil (Bahia, São Paulo, Rio de Janeiro).</p>Published as part of <i>Cruz, Estevam L., Silva, Da & Lise, Arno A., 2010, Taxonomic notes on the genus Paratrechalea Carico, 2005 (Araneae: Trechaleidae: Trechaleinae), pp. 43-50 in Zootaxa 2667</i> on pages 44-50, DOI: <a href="http://zenodo.org/record/276384">10.5281/zenodo.276384</a>
Investigation of new ligands for the antimicrobial peptide LL-37 using the Phage Display technique
No full textPeptídeos Antimicrobianos (AMPs) são moléculas-chave da imunidade inata. Os mecanismos celulares regulados por tais moléculas e o seu modo de ativação, no entanto, permanecem ainda obscuros. Catelicidinas são uma família de peptídeos antimicrobianos, expressas por células do sistema imune e por células epiteliais do intestino, vias aéreas, sistema uro-genital, superfície ocular e da pele. LL-37 é o único membro da família das catelicidinas em humanos. LL-37 é uma molécula capaz de matar microorganismos por ação direta, sendo também capaz de modular a resposta imune de maneira variada e ainda pouco compreendida. Phage Display é uma técnica de clonagem que se utiliza de uma biblioteca de pequenos peptídeos, expressos na superfície de partículas virais (bacteriófagos), para a captura de molécula-alvo. O presente estudo buscou identificar novos ligantes do peptídeo antimicrobiano LL-37, através da técnica de phage Display. Como resultado, descobrimos que a sequência de aminoácidos GNWSFV se liga ao peptídeo antimicrobiano LL-37. Alinhamento por bioinformática revelou que essa sequência apresenta grande similaridade com uma porção da proteína humana Spinster Homolog 3. Tal resultado desvenda um novo mecanismo de sinalização pelo peptídeo LL-37, podendo levar ao desenvolvimento de novas drogasAntimicrobial Peptides (AMPs) are essential components of the innate immunity. Their cellular mechanisms and mode of activation, however, remain unclear. Cathelicidins are a family of antimicrobial peptides expressed by cells of the immune system and by epithelial cells of the intestine, airways, genitourinary tract, ocular surface, and the skin. In humans, LL-37 is the only member of the cathelicidins family. LL-37 can kill microorganisms directly, being also able to modulate the immune response in various ways that are still poorly understood. Phage Display, a cloning technique, utilizes a library of small peptides, expressed on the surface of bacteriophages, to capture a target molecule. In this study, we aimed to identify new ligands of the antimicrobial peptide LL-37 using the phage display technique. Our results showed that the GNWSFV amino acids sequence binds to the antimicrobial peptide LL-37. Bioinformatics search revealed that this sequence bears great similarity to a portion of the human protein Spinster Homolog 3, uncovering a new LL-37 signaling mechanism that may be exploited to the development of new drug
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