44 research outputs found
O processo de abrasileiramento da igreja dos alemães
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas. Programa de Pós-Graduação em HistóriaAnálise, sob a perspectiva da História Oral, da trajetória da Igreja Evangélica de Confissão Luterana no Brasil (IECLB) em busca de sua identidade em terras brasileiras, iniciada após o baque da nacionalização e da II Guerra. A pesquisa recorre a autores, historiadores - eclesiásticos ou não - que trilharam o passado dessa igreja, quando ela se entendia como de rito alemão. Mas também se vale, e quer "salvar" a memória de atores, ainda vivos, que estiveram no palco do processo de abrasileiramento dessa igreja que até os dias de hoje ainda é vista, em muitos lugares do Sul do Brasil, como dos alemães. É bem verdade que ela substituiu o alemão pelo português no seu linguajar litúrgico. Para tanto, recebeu, porém, mais impulsos de fora do que foi a sua vontade interior de falar o português, inserir-se na realidade brasileira e trocar o adjetivo lutherano por luterano
Estudio de la actividad biológica de alquilglicerol lípidos análogos a compuestos de origen marino contra aislados de origen clínico
Get PDFIn the present study we evaluated the anti-biofilm and anti-quorum sensing activity against 8 clinical isolates and 3 reference strains, using 15 alkylglycerols obtained by chemical synthesis, they are analogous to those present in nature. In the first step, we evaluated the growth inhibitory activity of each alkylglycerol in order to quantify the anti-biofilm activity using 50%, 25% and 12.5% of the MIC concentration through broth microdilution. Using the modified Stepanovic protocol, we obtained biolfilm inhibitions of up 83.9%, which varied according to the alkylglycerol concentration and bacterial genus. The production of violacein of Chromobacterium violaceum ATCC 12472 was used for demonstrating the anti-quourum sensing activity by mean of diffusion on agar and broth microdilution techniques, depending on concentration and technique used; some alkylglycerols led to a lower production of violacein from C. violaceum ATCC 12472.
According to these results, alkylglycerols showed different effects depending on their concentration, the microorganism used and the number of chain carbon atoms
and their unsaturation’s.En el presente estudio se evaluó la capacidad anti-biofilm y anti-quorum sensing contra 8 aislados clínicos y 3 cepas de referencia, usando 15 alquilglicerol lípidos obtenidos mediante síntesis química, los cuales son análogos a los presentes en la naturaleza.
En primer lugar, se evaluó la capacidad inhibitoria de crecimiento de cada compuesto, para luego cuantificar la actividad anti-biofilm usando concentraciones al 50%, 25% y 12.5% de la MIC, mediante la técnica de microdilución en caldo. Utilizando el protocolo de Stepanovic modificado, se obtuvieron inhibiciones de la biopelícula de hasta un 83,9% las cuales variaban de acuerdo a la concentración y al género bacteriano. La producción de violaceína por parte de Chromobacterium violaceum ATCC 12472, fue usada para comprobar la actividad anti-quorum sensing mediante las técnicas de difusión en agar y microdilución en caldo; de acuerdo a la concentración y a la metodología usada, algunos compuestos generaban una disminución en la producción de violaceína por parte de la bacteria. De acuerdo a los resultados de los ensayos presentados en el documento, los alquilglicerol lípidos presentaron diferentes efectos dependientes de la concentración del compuesto, el microorganismo usado y el número de átomos de carbono e insaturaciones de la moléculaProductos Naturales Vegetales Bioactivos y Química EcológicaMagíster en Ciencias - Microbiología. Línea de Investigación: Comunicación BacterianaMaestrí
Development of a continuous culture tool for the generation of mutants induced by exposure to antibacterial agents
No full textilustraciones, fotografías, gráficas, tablasSe estableció un cultivo continuo operado al 50% de la velocidad específica de
crecimiento (= 0,2587 h
-1 para E. coli; = 0,2695 h
-1 para S. aureus), una vez
estabilizado el crecimiento se adicionó Piperacilina/ Tazobactam a una concentración
equivalente al 50% de la MIC hasta alcanzar la estabilidad de crecimiento. Luego, se
aumentó la concentración de Piperacilina/ Tazobactam a 75% de la MIC hasta
estabilidad y finalmente se incrementó hasta 1MIC. Con la herramienta propuesta, se
logró la generación de microorganismos mutantes, resistentes a Piperacilina/
Tazobactam (32 veces más resistentes tanto para E. coli como para S. aureus), con el
cultivo continuo en funcionamiento por aproximadamente 55 horas, de las cuales más o
menos 40 horas correspondieron a exposición antibiótica, lo que es un tiempo
relativamente corto y requiriendo un gasto bajo de material (caldo de cultivo gastado por
hora) con una alta producción de biomasa (microorganismos resistentes a Piperacilina/
Tazobactam).A continuous culture operated at 50% of the specific growth rate (= 0,2587 h
-1
for E. coli;
= 0,2695 h
-1
for S. aureus) was established. Once growth had stabilized,
Piperacillin/Tazobactam was added to a concentration equivalent to 50% of the MIC until
growth stability is reached. Piperacillin/Tazobactam concentration was then increased to
75% of the MIC until stable and finally increased to 1MIC. With the proposed tool, the
generation of mutant microorganisms resistant to Piperacillin/Tazobactam (32 times more
resistant to both E. coli and S. aureus) was achieved, with continuous culture in operation
for approximately 55 hours, of which more or less 40 hours corresponded to antibiotic
exposure, which is a relatively short time and requires a low expenditure of material
(culture broth spent per hour) with a high production of biomass (Piperacillin/Tazobactam
resistant microorganisms).MaestríaMagíster en Ciencias - FarmacologíaFarmacologí
Contemporary theory of metaphor: an analysis of lakoffian echoes in Brazil
Get PDFDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Comunicação e Expressão, Programa de Pós-Graduação em Letras/Inglês e Literatura Correspondente, Florianópolis, 2010Nos últimos trinta anos, a metáfora tem sido observada sob aspectos mais psicológicos e cognitivos, tornando-se objeto de interesse central nas ciências humanas, principalmente na psicologia cognitiva que tem desenvolvido uma grande quantidade de pesquisas dedicadas ao processo de compreensão da metáfora. O objetivo deste estudo é contribuir com um mapeamento das diferentes perspectivas dos pesquisadores brasileiros dedicados à Teoria Contemporânea da Metáfora. Os dados consistem de trezentos e cinco estudos empíricos publicados entre 1998 e março, 2010. A análise mostra que (1) São Paulo foi o estado brasileiro mais frutífero a desenvolver pesquisas no tema e PUC-SP foi a universidade com o maior número de publicações, seguida pela UFJF e a UFSC. Também o GEIM - Grupo de Estudos da Indeterminação e da Metáfora - demonstrou ser o grupo de pesquisa com contribuições mais significantes para o campo; (2) a maioria dos estudos foi conduzida em português como língua materna - principalmente os baseados em Lingüística de Corpus; (3) a investigação brasileira tem sido conduzida de forma qualitativa e possui abundantes pesquisas etnográficas e de ação; (4) há uma tendência em favor do uso da metodologia de Lingüística de Corpus com ênfase no uso das ferramentas fornecidas pelos Laboratórios de língua brasileiros; (5) existe um leque de investigadores que têm observado a metáfora conceitual sob uma perspectiva mais filosófica e semântica; (6) a maioria dos pesquisadores selecionados neste estudo analisaram a indeterminação de significados na língua (real) em uso; (7) Metaphors we live by (Lakoff & Johnson 1980) e sua tradução ao português - pelo GEIM - demonstrou ser indispensável na literatura brasileira. Resultados gerais sugerem que Brasil é um campo fértil e tem explorado a abordagem Lakoffiana da metáfora nas mais diversas áreas de estudo
An Approach to Enable Human–3D Object Interaction Through Voice Commands in an Immersive Virtual Environment
No full textNowadays, the Metaverse is facing many challenges. In this context, Virtual Reality (VR) applications allowing voice-based human–3D object interactions are limited due to the current hardware/software limitations. In fact, adopting Automated Speech Recognition (ASR) systems to interact with 3D objects in VR applications through users’ voice commands presents significant challenges due to the hardware and software limitations of headset devices. This paper aims to bridge this gap by proposing a methodology to address these issues. In particular, starting from a Mel-Frequency Cepstral Coefficient (MFCC) extraction algorithm able to capture the unique characteristics of the user’s voice, we pass it as input to a Convolutional Neural Network (CNN) model. After that, in order to integrate the CNN model with a VR application running on a standalone headset, such as Oculus Quest, we converted it into an Open Neural Network Exchange (ONNX) format, i.e., a Machine Learning (ML) interoperability open standard format. The proposed system demonstrates good performance and represents a foundation for the development of user-centric, effective computing systems, enhancing accessibility to VR environments through voice-based commands. Experiments demonstrate that a native CNN model developed through TensorFlow presents comparable performances with respect to the corresponding CNN model converted into the ONNX format, paving the way towards the development of VR applications running in headsets controlled through the user’s voice
La función notarial y la prueba testimonial: ¿posibilidades para la descongestión judicial?
Get PDFFrente al objetivo general establecido en el presente trabajo, se puede considerar que el testimonio ante notaría, como prueba anticipada, no se puede aplicar, en el entendido que al procurar la verdad de los hechos y ante la imposibilidad fáctica de que un juez pueda practicarla, no es plausible que se pudiera practicar, pues ello implicaría traspasar las fronteras de la función notarial e invadir, en consecuencia, la órbita jurisdiccional. Si bien el escenario puesto por el Consejo de Estado se daba en otro contexto, el estudio no logra contemplar con firmeza la aplicabilidad ante notarios, ya que estos no tendrían las competencias jurídicas y condiciones técnicas para dar fe de lo que se diga en el testimonio para que, ulteriormente, sea el juez quien, bajo su función jurisdiccional, determine si es simplemente un indicio, una prueba sumaria o plena prueba. Ahora, la prueba testimonial anticipada con fines judiciales permite entender también que existen criterios, reglas y principios que se deben conservar como parte integral del derecho al debido proceso. En este sentido, se concluye que el testimonio como medio de prueba, debe cumplir con todos los criterios legales y jurisprudenciales analizados en el presente trabajo, entre los que se identifica que se deben llevar a cabo bajo gravedad de juramento; que se garantice la aplicación de los principios de inmediación, concentración y contradicción; que si el testimonio no es ratificado en sede judicial durante el proceso, se entiende únicamente como prueba sumaria o como indicio; que el testigo necesariamente debe ser un tercero extraño al proceso, es decir que no represente intereses para las partes y se pueda tachar; que en el testimonio se deben exponer de forma clara las circunstancia de tiempo, modo y lugar de los hechos objeto de ulterior demanda o denuncia; y que se puedan practicar en aquellos casos en los cuales se encuentren personas gravemente enfermas. Por último, se puede concluir que la prueba testimonial anticipada con fines judiciales es un derecho constitucional que tienen las partes, pero que el mismo se debe contemplar bajo premisas y eventos específicos que, excepcionalmente (como el propuesto en el acápite anterior), se deberían contemplar para su aplicación ante notarios, siempre bajo criterios constitucionales y legales que favorezcan el debido proceso en el marco del derecho administrativo.Faced with the general objective established in the present work, it can be considered that the testimony before notary, as advance evidence, can not be applied, in the understanding that in seeking the truth of the facts and the factual impossibility that a judge can practice it , it is not plausible that it could be practiced, since this would imply crossing the borders of the notarial function and invading, as a consequence, the jurisdictional orbit. Although the scenario put by the State Council was in another context, the study fails to firmly contemplate the applicability before notaries, since they would not have the legal powers and technical conditions to attest to what is said in the testimony so that, later, it is the judge who, under his jurisdictional function, determines if it is simply an indication, a summary test or full proof. Now, the anticipated testimonial evidence for judicial purposes also allows us to understand that there are criteria, rules and principles that must be kept as an integral part of the right to due process. In this sense, it is concluded that the testimony as a means of proof, must comply with all the legal and jurisprudential criteria analyzed in this work, among which it is identified that they must be carried out under the seriousness of an oath; that the application of the principles of immediacy, concentration and contradiction be guaranteed; that if the testimony is not ratified in the courtroom during the process, it is understood only as summary evidence or as evidence; that the witness must necessarily be a third party alien to the process, that is, that it does not represent interests for the parties and can be crossed out; that in the testimony the circumstance of time, manner and place of the events subject to further demand or denunciation must be clearly stated; and that can be practiced in those cases in which people are seriously ill. Finally, it can be concluded that the anticipated testimonial evidence for judicial purposes is a constitutional right that the parties have, but that it must be contemplated under specific premises and events that, exceptionally (as proposed in the previous section), should be contemplate for its application before notaries, always under constitutional and legal criteria that favor due process within the framework of administrative law
Os Estudantes e o mundo da política: análise das experiências políticas dos estudantes da Universidade de São Carlos da Guatemala na perspectiva do drama ritual
Get PDFDissertação (mestrado) - Unversidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas, Programa de Pós-Graduação em Antropologia Social, Florianópolis, 2011A presente dissertação consiste numa etnografia das experiências políticas entre os estudantes da Universidade de São Carlos da Guatemala. Nela, festas, greves, caminhadas, processos eletivos e outros acontecimentos em que os estudantes disputam o poder, dentro da Universidade e fora dela, são analisados como eventos críticos que expressam dramaticamente os principais elementos que configuram o seu "mundo da política" e, ao mesmo tempo, contribuem para a configuração dos estudantes universitários sancarlistas como sujeitos políticos. A perspectiva segundo a qual são analisadas as experiências focaliza a noção de drama social, conforme desenvolvida pelo antropólogo Victor Turner. O trabalho busca, por um lado, contribuir para uma compreensão mais aprofundada da atividade política estudantil universitária a partir do modo como os próprios sujeitos compreendem e experimentam o seu mundo social, dos sentidos que lhe atribuem e das formas como interagem nele; por outro lado, pretende despertar o interesse analítico sobre estes sujeitos que têm sido historicamente identificados como atores fundamentais na vida política da Guatemala.This dissertation is an ethnography on the political experiences among students of the University of San Carlos de Guatemala. In her holidays, strikes, walks, elective procedures and other events where students vying for power within the university and beyond are addressed as critical events that dramatically express the main elements that form their "world politics" and at the same time, contribute in shaping college students sancarlistas as political subjects. The view from where they are analyzed the experiences focuses the notion of social drama, as developed by the anthropologist Victor Turner. The work aims on the one hand, contribute to a deeper understanding of political activity from university student as the subjects themselves understand and experience their social world, the meanings attributed to it and the ways they interact in it, while, furthermore, analytical arouse interest on these subjects that have historically been identified as key actors in the political life of Guatemala
Sciopemyia apicalis Chaves Junior, Shimabukuro & Andrade 2022, sp. n.
No full textSciopemyia apicalis Chaves Júnior, Shimabukuro & Andrade sp. n. (Figs 5–6) Diagnosis. Male: preapical papilla on flagellomere III present; gonocoxite without basal tuft of setae; flagellomere I ≥ 405 µm; aedeagal ducts ≤ 440 µm; epandrial lobe ≤ 157 µm; presence of one apical spine and upper external spine at the apical level in the gonostyle. Male holotype. Head (Fig. 5A) 330 (320–330, n = 6) in length, 264 (255–304, n = 6) in width. Arrangement of deciduous bristles in the occiput region forming an “X” shape; clypeus 119 (107–118, n = 7) in length; eyes 154 (133–159, n = 6) in length, 96 (70–93, n = 6) in width; interocular distance 122 (101–125, n = 5). Pharynx with streaks and no teeth (Fig. 5B). Cibarium without teeth (Fig. 5B). Labrum-epipharynx (LE) 136 (142–148, n = 6) (Fig. 5C). Incomplete antenna on type specimen, description based on paratype (Nº 32847). Flagellomere XIII and FXIV absent in all specimens. Antenna (Figs 5D–F, 6A–I): flagellomere length (F): FI 442 (349–474, n = 6), FII 225 (172–228, n = 6), FIII 229 (172–228, n = 6), FXIII (lost), FXIV (lost). Ascoids: with short posterior spur and atrophied peduncle, anterior spur is long in FI and reaches the level of the preapical papilla (Fig. 5D) but does not reach FII; ascoids: implanted at the same level as the internal; flagellomere FX, FXI and FXII absent in type specimen, description based on paratype (Nº 32847). Presence of preapical papilla on FI– FIII (Figs 5D–F); papilla absent on FIV–FVI (Figs 6A–C); presence of one papilla on FVII, FVIII and FIX (Figs 6D–F), two papillae on FX (Fig. 6G), three papillae on FXI (Fig. 6H); presence of four papillae on FXII (Fig. 6I); spiniform preapical papilla on FIX (Fig. 6F) and FXI (Figs 6H). Presence of simple setae on FVII – FXII (Figs 6A–I). Palpi (P) (Fig. 5G), palpal segment length: PI 32 (29–35, n = 6), PII 58 (55–64, n = 6), PIII 101 (92–116, n = 6), PIV 61 (64–70, n = 6) and PV 128 (107–130, n = 5). Palpal formula: 1-2-4-3-5 (n = 5); PIII with Newstead’s sensilla scattered along the segment (Fig. 5G). Labial suture complete (Fig. 5A). Cervix. Ventro-cervical sensilla absent. Cervical sclerites bearing a pair of spiniform sensilla. Thorax. Mesonotum 409 (372–457, n = 6) in length; pronotoum, anepisternum, metanotum and postnotum light brown, paratergite and pleura off–white. One proepimeral setae; 9–11 upper anepisternal setae. Setae present on the anterior margin of the katepisternum. Wing (Fig. 6J): 1,794 (1,461 –1,905, n = 5) in length, 445 (416–525, n = 5) in width; veins: R 5 1,091 (1,036 –1,128, n = 5); alfa 399 (320–404, n = 5); beta 246 (197–284, n = 5); gamma 197 (194–255, n = 5); delta 90 (41–113, n = 5); pi 95 (51–117, n = 5). Legs (anterior, median, posterior): Coxae: 279 (288–316, n = 6), 279 (288–302, n = 6), 279 (293–316, n = 6); Femur: 759 (642–774, n = 3), 715 (598–737, n = 5), 723 (657–795, n = 4); Tibia: 1,147 (943–1,221, n = 3), 1,258 (1,091 –1,406, n = 5), 1,276 (1,147 –1,480, n = 3); Tarsomere I: 672 (591–723, n = 3), 715 (650–803, n = 5), 752 (679–854, n = 3). Sum of tarsomeres II+IV+ V (anterior, median, posterior): 644 (584–679, n = 3), 693 (605–715, n = 5), 715 (657–715, n = 3). Abdomen: 1,720 (1,128 –1,720, n = 6) in length; tergal papillae absent. Terminalia (Fig. 6K): gonocoxite 154 (145–154, n = 6) in length, 58 (52–61, n = 6) in width, without basal tuft of setae. Gonostyle 136 (122–133, n = 7) in length, without preapical seta and with four spines: one apical, one upper external that migrates towards the apex, one lower external, and one internal spine. The four spines are well-developed. The lower external spine is located at a level closer to the apex spines than the internal. Internal spine located in the apical third of the gonostylus. Paramere (Fig. 6K): dorsal margin 110 (90–133, n = 6) and ventral 128 (124–159, n = 6) in length; the basal level of the bristles in the dorsal margin reaches the apical level of the implantation of the bristles in the ventral margin (Fig. 6K). Parameral sheath sclerotized and coniform. Epandrial lobe 154 (145–177, n = 6) in length, 29 (20–38, n = 6) in width and rounded at the apex. Sperm pump (Fig. 6L) 130 (101–130, n = 6); ejaculatory apodeme 96 (81–101, n = 6); aedeagal ducts with bevelled apex (Fig. 6L), 394 (375–414, n = 6) in length and 3 (3, n = 6) in width; 2,7 times the length of sperm pump. Cercus 96 (107–130, n = 6) in length and 43 (40–58, n = 6) in width. Material examined: Holotype (Nº 32827) and two paratypes (Nº 32832, 32847). Capture location: Fazenda São Geraldo, house of Mr. José Marques dos Santos, municipality of Monsenhor Paulo (21°45′30.14″ S, 45°32′22.15″ W), state of Minas Gerais, Brazil. Collection date: uninformed. Trap: uninformed, Collectors: Antônio Leôncio and Eduardo. Identified as Lu. nordestina. Paratypes from other locations: 1 male (Nº 49201). Capture location: Engenho de São Francisco da Várzea (8º03′27″ S, 34º57′52″ W), municipality of Recife, state of Pernambuco, Brazil. Collection date: 24/ix/1974, from 1:00 p.m. to 4:00 p.m. Trap: manual capture in wild animal burrows (termites) in closed florest. Collectors: João Evangelista da Silva. Determined by Amilcar Vianna Martins as L. nordestina in October 1974. 1 male (Nº 64671). Capture location: “Sítio do Sr. Alencar” municipality of Maranguapé (3º52′58.62″ S, 38º40′36.20″ W), state of Ceará, Brazil. Collection date: 17/x/1983, from 11:00 a.m. to 11:30 a.m. Trap: Manual capture in uninhabited shelters approximately 3 m away from the water collection, feeding on frogs. Collectors: Edelberto Santos Dias. Determined by Alda Lima Falcão and Armando Lemos Ribeiro as L. nordestina. 1 male (Nº 71674). Capture location: municipality of Monte do Carmo (Nº 10º47′12.71″ S, 48º00′03.70″ W), state of Tocantins, Brazil. Collection date: i/1998. Trap: uninformed. Collector: W. A. Andrade. 1 male (Nº 86806). Capture location: Caverna Hall two of Caverna do Arrebenta Bomba, localized in the municipality of Lassance (17º53′22.31″ S, 44º34′53.63″ W), state of Minas Gerais, Brazil. Collection date: 15/iv/2008, from 2:00 p.m. (16/iv/2008) to 2:00 p.m.(17/iv/2008). Trap: uninformed. Collector: uninformed. Determined by Gustavo Mayr de Lima Carvalho as Lu. sordellii in May 2008. Material deposited in the FIOCRUZ/COLFLEB. Female: Unknown. Etymology: the species name refers to the position of the upper external spine, which is located more at the apex of the gonostyle, different from the males of Sc. sordellii. Distribution: BRAZIL. Ceará: Maranguapé; Minas Gerais: Lassance, Monsenhor Paulo, Nova Lima; Pernambuco: Recife; Tocantins: Monte do Carmo. Medical importance: Studies have not yet been conducted on this species regarding vector competence or capacity, or natural infection by parasites.Published as part of Chaves Júnior, Salvador P., Shimabukuro, Paloma H. F. & Andrade, Andrey J., 2022, Description of the female and redescription of the male of Sciopemyia sordellii (Shannon & Del Ponte, 1927), including the description of four new species of the genus Sciopemyia Barretto, 1962 (Diptera: Psychodidae: Phlebotominae) from Brazil, pp. 301-336 in Zootaxa 5195 (4) on pages 312-316, DOI: 10.11646/zootaxa.5195.4.1, http://zenodo.org/record/719873
