8,038 research outputs found
Pioneers of Library Movement in Pakistan
No full textThe paper aims to describe in brief the contribution of seven leaders of Pakistan librarianship, viz. K.B. Khalifa M. Asadullah, Prof. Dr. Abdul Moid, Dr. Abdus Subuh Qasimi, Muhammad Shafi, Fazal Elahi, Khawaja Nur Elahi and S. V. Hussain. The early library developments are given for better understanding of the role of these leaders
Circulating tumor DNA and liquid biopsy: opportunities, challenges, and recent advances in detection technologies.
No full textCell-free DNA (cfDNA) refers to short fragments of acellular nucleic acids detectable in almost all body fluids, including blood, and is involved in various physiological and pathological phenomena such as immunity, coagulation, aging, and cancer. In cancer patients, a fraction of hematogenous cfDNA originates from tumors, termed circulating tumor DNA (ctDNA), and may carry the same mutations and genetic alterations as those of a primary tumor. Thus, ctDNA potentially provides an opportunity for noninvasive assessment of cancer. Recent advances in ctDNA analysis methods will potentially lead to the development of a liquid biopsy tool for the diagnosis, prognosis, therapy response monitoring, and tracking the rise of new mutant sub-clones in cancer patients. Over the past few decades, cancer-specific mutations in ctDNA have been detected using a variety of untargeted methods such as digital karyotyping, personalized analysis of rearranged ends (PARE), whole-genome sequencing of ctDNA, and targeted approaches such as conventional and digital PCR-based methods and deep sequencing-based technologies. More recently, several chip-based electrochemical sensors have been developed for the analysis of ctDNA in patient samples. This paper aims to comprehensively review the diagnostic, prognostic, and predictive potential of ctDNA as a minimally invasive liquid biopsy for cancer patients. We also present an overview of current advances in the analytical sensitivity and accuracy of ctDNA analysis methods as well as biological and technical challenges, which need to be resolved for the integration of ctDNA analysis into routine clinical practice
An amplification-free electrochemical detection of exosomal miRNA-21 in serum samples
No full textRecent evidence suggests that small non-coding RNAs or microRNA (miRNA)s encapsulated in exosomes represent an important mechanism of communication between the cells. Exosomal miRNAs play an important role in oncogenesis via stimulating cell to cell communication and facilitating metastasis in cancers. Despite progressive advances, current methods for exosomal miRNA detection most rely on labor intensive sequencing approaches which are often prone to amplification bias and require costly and bulky equipment. Herein, we report an electrochemical approach for detection of cancer-derived exosomal miRNAs in human serum samples by selectively isolating the target miRNA using magnetic beads pre-functionalized with capture probes and then directly absorbing the targets onto the gold electrode surface. The level of adsorbed miRNA is detected electrochemically in the presence of the [Fe(CN)6]4-/3- redox system. This method enabled an excellent detection sensitivity of 1.0 pM with a relative standard deviation (%RSD) of <5.5% in cancer cells and serum samples (n = 8) collected from patients with colorectal adenocarcinoma. We believe that our approach could be useful for the quantification of exosomal miRNA in clinical samples.Griffith Sciences, School of Natural SciencesNo Full Tex
Detecting DNA methylation for cancer diagnostics and prognostics
No full textDNA methylation is an epigenetic process that has roles in many normal cellular processes and dysregulation of which can result in diseases such as cancer. The most well studied form of mammalian DNA methylation is the addition of a methyl group to the number 5 carbon of cytosine in CpG dinucleotides. Gene regulatory elements, such as gene promoters and enhancers associated with dense CpGs, are sensitive to DNA methylation silencing. Multiple studies have shown that promoter methylation profiling of cancer genes may be useful as biomarkers of cancer. DNA methylation information has the potential to provide information on a patient's cancer subtype, treatment response and prognosis. Current DNA methylation detection techniques can be broadly grouped into sodium bisulfite, restriction enzyme and methylated DNA enrichment based techniques. However, techniques for detection DNA methylation have largely been tailored to research purposes and may not be well suited for routine clinical use. In this chapter, some of these common DNA methylation detection methods are reviewed for their suitability in diagnostics. Also discussed are ways how some of these techniques may be or have been adapted for clinical and point-of-care applications. Emerging techniques that have evolved from classical research methods are also introduced
The Complete Muhammad Ali
No full textIncluding material and photographs not included in most of the 100 other books about the champion, Ishmael Reed's The Complete Muhammad Ali is more than just a biography-it is a fascinating portrait of the 20th century and the beginning of the 21st. An honest, balanced portrayal of Ali, the book includes voices that have been omitted from other books. It charts Ali's evolution from Black Nationalism to a universalism, but does not discount the Nation of Islam and Black Nationalism's important influence on his intellectual development. Filipino American author Emil Guillermo speaks about how "The Thrilla' In Manila" brought the Philippines into the 20th century. Fans of Muhammad Ali, boxing fans, and those interested in modern African American history and the Nation of Islam will be fascinated by this biography by an accomplished American author.Intro -- DEDICATION -- INTRODUCTION -- The Curious History of an Icon -- CHAPTER 1 -- CHAPTER 2 -- CHAPTER 3 -- CHAPTER 4 -- CHAPTER 5 -- CHAPTER 6 -- CHAPTER 7 -- Did the Secret Government Fear a U.S. Muslim/Overseas Muslim Alliance? -- CHAPTER 8 -- CHAPTER 9 -- The Break Between the Prophet and his Disciple -- CHAPTER 10 -- CHAPTER 11 -- CHAPTER 12 -- The GOAT (Greatest Of All Time): Ali or Louis? -- CHAPTER 13 -- The Nation of Islam, the Mob, Showdowns in Canada and Sonny Liston -- CHAPTER 14 -- CHAPTER 15 -- The Taunts: Marketing or Racism? -- CHAPTER 16 -- CHAPTER 17 -- CHAPTER 18 -- CHAPTER 19 -- Boxing and the Brain -- CHAPTER 20 -- Ali's Feet -- CHAPTER 21 -- Mr. Dick -- CHAPTER 22 -- CHAPTER 23 -- The Opening Ceremonies, November 2005 -- CHAPTER 24 -- December 2005, Las Vegas -- CHAPTER 25 -- CHAPTER 26 -- June 16, 2004 -- CHAPTER 27 -- CHAPTER 28 -- CHAPTER 29 -- Aix-en-Provence -- CHAPTER 30 -- Ali as a Black Nationalist -- San Francisco, January 2004 Black Liberation Book Fair -- CHAPTER 31 -- January 31, 2004 -- CHAPTER 32 -- October 2005, Chicago -- CHAPTER 33 -- Why Ali remained with Elijah instead of following Malcolm -- CHAPTER 34 -- CHAPTER 35 -- February 4, 2006, Oakland, California -- CHAPTER 36 -- Like Zeus Descending from Mount Olympus -- CHAPTER 37 -- CHAPTER 38 -- Tuesday, February 28, 2006, New York -- CHAPTER 39 -- Bigger Than Boxing -- CHAPTER 40 -- Tribes Gallery, New York, April 2006 -- CHAPTER 41 -- June 2006, Louisville, Kentucky -- CHAPTER 42 -- CHAPTER 43 -- CHAPTER 45 -- Bad Company -- CHAPTER 46 -- Coxson, A Very Charming Rogue -- CHAPTER 47 -- Ali and the largest embezzlement scheme in Wells Fargo history -- CHAPTER 48 -- CHAPTER 49 -- "Lonnie is a stabilizing force."-Harry Belafonte -- October 29, 2006 -- CHAPTER 50 -- Abdul Rahman -- CHAPTER 51 -- CHAPTER 52 -- CHAPTER 53How Will Ali Be Remembered? New York, January 8, 2005 -- CHAPTER 54 -- CONCLUSION -- AFTERWORD -- Boxers' Rights? -- BIBLIOGRAPHY -- MUHAMMAD ALI -- ISLAM AND NATION OF ISLAM -- BOXING -- RELATED SUBJECTS -- ALSO AVAILABLE FROM BARAKA BOOKSIncluding material and photographs not included in most of the 100 other books about the champion, Ishmael Reed's The Complete Muhammad Ali is more than just a biography-it is a fascinating portrait of the 20th century and the beginning of the 21st. An honest, balanced portrayal of Ali, the book includes voices that have been omitted from other books. It charts Ali's evolution from Black Nationalism to a universalism, but does not discount the Nation of Islam and Black Nationalism's important influence on his intellectual development. Filipino American author Emil Guillermo speaks about how "The Thrilla' In Manila" brought the Philippines into the 20th century. Fans of Muhammad Ali, boxing fans, and those interested in modern African American history and the Nation of Islam will be fascinated by this biography by an accomplished American author.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Quantum dot-based sensitive detection of disease specific exosome in serum
No full textTumor-derived exosomes have emerged as promising cancer biomarkers due to their unique composition and functions. Herein, we report a stripping voltammetric immunoassay for the electrochemical detection of disease-specific exosomes using quantum dots as signal amplifiers. The assay involves three subsequent steps where bulk exosome populations are initially magnetically captured on magnetic beads by a generic tetraspanin antibody (e.g., CD9 or CD63) followed by the identification of disease-specific exosomes using cancer-related. Here, we used CdSe quantum dot (CdSeQD) functionalised-biotinylated HER-2 and FAM134B antibodies as breast and colon cancer markers. After magnetic washing and purification steps, acid dissolution of CdSeQDs and subsequent anodic stripping voltammetric quantification of Cd2+ were carried out at the bare glassy carbon working electrode. This method enabled sensitive detection of 100 exosomes per μL with a relative standard deviation (%RSD) of <5.5% in cancer cell lines and a small cohort of serum samples (n = 9) collected from patients with colorectal adenocarcinoma. We believe that our approach could potentially represent an effective bioassay for the quantification of disease-specific exosomes in clinical samples.Griffith Sciences, School of Natural SciencesNo Full Tex
Nilai Pendidikan Karakter Dalam Kitab Washaya Al-Aba’ Li Al-Abna Karya Muhammad Syakir Al-Iskandari
No full textThis research aims to identify the character education values contained in the book Washaya al-Aba' li al-Abna Muhammad Syakir and the role of his opinion on the richness of character education values. It is hoped that this research can be used as a source or reference in efforts to develop education and character as a whole, especially character education according to Islam. It can also be used as a reference to facilitate understanding of character education and as a reference for relevant research in the future. This research method is qualitative and uses a narrative biography method. By using a descriptive presentation approach, this autobiography of the character's thoughts combines the ideas of character education from his work. Library research, or library research, is a research method that involves books, articles, notes and electronic media. with primary sources from the book Washaya al Aba Li al-Abna and secondary sources from study books. In this research, data collection was carried out through the documentation method, which includes searching for data through variables such as notes, books, and articles, among others. The author used a content analysis study to analyze the data; they analyzed the data textually based on the contents of the book. The research results show that the book Washaya al Aba Li al-Abna contains 17 educational character values, divided into two categories: 10 character values fall into the moral category and 7 character values fall into the performance category. Faith and piety, love and obedience to the Messenger of Allah, respect for parents, respect for teachers, truth or honesty, nobility or self-respect, patience, sincerity and a simple life are moral characters. Performance characteristics also include trust, discipline, hard work, never giving up, love of the country, interest in reading or literacy knowledge, and concern for the environment
Biography of Muhammad Baba
No full textCe manuscrit est une biographie du savant islamique Muhammad Baba du village de Doumga, au Sénégal. Elle fait partie d'une série de biographies que l'auteur a écrites sur les dirigeants islamiques en Afrique de l'Ouest.This manuscript is a biography of Islamic scholarMuhammad Baba of the village of Doumga, in Senegal. It is one of a series of biographies that the author has written about Islamic leaders in West Africa
Some Letters by Muhammad al-Ghāli Ba
No full textCe manuscrit comprend des correspondances et un article. Les correspondances étaient destinées à plusieurs personnes. L'article a été réécrit lorsque l'auteur, Muhammad al-Ghali, travaillait à la radio nationale mauritanienne. L'article traite de la situation politique de la Mauritanie dans les années 80. Il discute des relations entre les partis politiques existants en Mauritanie.This manuscript includes correspondences and one article. The correspondences had been destined to several people. The article was written back when the author, Muhammad al-Ghali, was working at Mauritania' s National Radio. The article addresses the political situation of Mauritania in the 1980s. It discusses the relationship between the existing political parties in Mauritanian
- …
