954 research outputs found

    Invariable biomass-specific primary production of taxonomically discrete picoeukaryote groups across the Atlantic Ocean

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    Oceanic photosynthetic picoeukaryotes (< 3 µm) are responsible for > 40% of total primary production at low latitudes such as the North-Eastern tropical Atlantic. In the world ocean, warmed by climate changes, the expected gradual shift towards smaller primary producers could render the role of photosynthetic picoeukaryotes even more important than they are today. Little is still known, however, about how the taxonomic composition of this highly diverse group affects primary production at the basin scale. Here, we combined flow cytometric cell sorting, NaH14CO3 radiotracer incubations and class-specific fluorescence in situ hybridization (FISH) probes to determine cell- and biomass-specific inorganic carbon fixation rates and taxonomic composition of two major photosynthetic picoeukaryote groups on a ?7500-km-long latitudinal transect across the Atlantic Ocean (Atlantic Meridional Transect, AMT19). We show that even though larger cells have, on average, cell-specific CO2 uptake rates ?5 times higher than the smaller ones, the average biomass-specific uptake is statistically similar for both groups. On the other hand, even at a high taxonomic level, i.e. class, the contributions to both groups by Prymnesiophyceae, Chrysophyceae and Pelagophyceae are significantly different (P < 0.001 in all cases). We therefore conclude that these group's carbon fixation rates are independent of the taxonomic composition of photosynthetic picoeukaryotes across the Atlantic Ocean. Because the above applies across different oceanic regions the diversity changes seem to be a secondary factor determining primary production

    Financial Summit: J.R. Briggs & Dave Briggs

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    Dave and J.R. Briggs speak on stewardship of finances. Dave Briggs currently serves as the stewardship director at Central Christian Church of Arizona. Previously he served in a similar role at Willowcreek Church and prior to that was a finance manager for GE for 27 years. He has developed numerous financial seminars and classes and regularly speaks at churches and conferences around the country. Dave and his wife Debbie had two sons attend Taylor and served for three years on the Taylor Parents’ Cabinet. J.R. Briggs wears a variety of ministry hats. On a local level, he serves as one of the pastors of The Renew Community. He is also the Founder and Director of Kairos Partnerships and Director of Leadership and Congregation Formation for the Ecclesia Network. He is a life coach, consultant, frequent speaker, and author of seven books. He and his wife Megan, along with their two sons Carter and Bennett, live in the Philadelphia area

    Rex J. Rowley

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    Audio recording of the 10/06/13 UNLV Libraries Author Series event featuring Rex. J. Rowley, author of Everyday Las Vegas: Local Life in a Tourist Town. Includes remarks by Libraries Dean Patricia Iannuzzi, CGR Director Dave Schwartz, and Rowley

    Analysis of photosynthetic picoeukaryote community structure along an extended Ellett Line transect in the northern North Atlantic reveals a dominance of novel prymnesiophyte and prasinophyte phylotypes

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    Photosynthetic picoeukaryotes (PPEs) of a size <3 mu m can contribute significantly to primary production. Here, PPE community structure was analysed along an extended Ellett Line transect, an area in the North Atlantic well studied by physical oceanographers but largely neglected in the field of microalgal ecology. Distribution patterns of specific PPE classes were determined using dot-blot hybridization analysis, while the taxonomic composition of specific PPE classes was revealed by phylogenetic analysis of plastid 16S rRNA gene sequences. In addition, we performed fluorescent in situ hybridization (FISH) analysis of seawater samples collected along the transect to provide a PCR-independent survey of class level PPE distribution patterns. We found the PPE community was dominated by members of the Prymnesiophyceae, Prasinophyceae and Mamiellophyceae. Interestingly, phylogenetic analysis revealed several novel Prymnesiophyceae and Prasinophyceae phylotypes (with only 85-96% identity to neighbouring sequences) within lineages for which cultured counterparts are unknow

    In situ interactions between photosynthetic picoeukaryotes and bacterioplankton in the Atlantic Ocean: evidence for mixotrophy

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    Heterotrophic bacterioplankton, cyanobacteria and phototrophic picoeukaryotes (< 5 μm in size) numerically dominate planktonic oceanic communities. While feeding on bacterioplankton is often attributed to aplastidic protists, recent evidence suggests that phototrophic picoeukaryotes could be important bacterivores. Here, we present direct visual evidence from the surface mixed layer of the Atlantic Ocean that bacterioplankton are internalized by phototrophic picoeukaryotes. In situ interactions of phototrophic picoeukaryotes and bacterioplankton (specifically Prochlorococcus cyanobacteria and the SAR11 clade) were investigated using a combination of flow cytometric cell sorting and dual tyramide signal amplification fluorescence in situ hybridization. Using this method, we observed plastidic Prymnesiophyceae and Chrysophyceae cells containing Prochlorococcus, and to a lesser extent SAR11 cells. These microscopic observations of in situ microbial trophic interactions demonstrate the frequency and likely selectivity of phototrophic picoeukaryote bacterivory in the surface mixed layer of both the North and South Atlantic subtropical gyres and adjacent equatorial region, broadening our views on the ecological role of the smallest oceanic plastidic protists

    From Pedrolino to a Pierrot: The Origin, Ancestry and Ambivalence of the British Pierrot Troupe

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    In this article, the author considers the British development of the seaside Pierrot troupe, arguing that its construction is consistent with the notion of invented tradition, and the associated concerns with identity and nationality. Tracing the history of the character from its origins as Pedrolino in the commedia dell’arte, the article considers the traditional and novel elements of the British form. This also allows a brief account of the origin and aesthetics of the British tradition. Reflecting on the synthesis of the archaic and contemporary dimensions of the form, the author proposes that the new structure constructed an ambivalent class of character. The composition of both troupes and audiences was drawn from across the range of social strata. Through its collectivity and its treatment of contemporary social themes, it is argued the British Pierrot troupe approached and negotiated questions of a cultural and national identity in the late-Victorian period. Dave Calvert is a Senior Lecturer at the University of Huddersfield, UK. His research interests include street theatre, Applied Theatre and learning disabled performance. He is also a member of The Pierrotters, the last remaining seaside Pierrot troupe

    Charles Kelly with A. R. Mortenson, Dave Bigler, and E. L. Cooley

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    A. R. Mortenson, Dave Bigler, E. L. Cooley presented an American Association for State and Local History award to Charles Kelly at Utah Westerner\u27s meeting. Charles Kelly was a printer, artist, author, historian, the first superintendent of Capitol Reef National Park

    Charles Kelly with A. R. Mortenson, Dave Bigler, and E. L. Cooley

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    A. R. Mortenson, Dave Bigler, E. L. Cooley presented an American Association for State and Local History award to Charles Kelly at Utah Westerner\u27s meeting. Charles Kelly was a printer, artist, author, historian, the first superintendent of Capitol Reef National Park

    Basin-scale distribution patterns of picocyanobacterial lineages in the Atlantic Ocean

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    Marine picocyanobacteria of the genera Prochlorococcus and Synechococcus are major contributors to oceanic primary production. The genera are genetically diverse, comprising several known ecotypes or lineages. However, little is known of the distribution of these lineages over large geographic areas. Here, we analysed the relative abundance of Prochlorococcus ecotypes and Synechococcus lineages at the ocean basin scale along an Atlantic Meridional Transect (AMT) using dot blot hybridization and fluorescence in situ hybridization (FISH) techniques. The transect covered several contrasting oceanic provinces (gyres, upwelling, temperate regions) as well as environmentally 'equivalent' regions in the northern and southern hemisphere (northern and southern gyres and temperate regions). Flow cytometric data revealed a discrete separation in abundance of major picocyanobacterial genera. Prochlorococcus reached highest abundance in oligotrophic regions, while more mesotrophic waters were dominated by Synechococcus. Individual genetic lineages of both Prochlorococcus and Synechococcus showed highly similar distributions in corresponding regions in the northern and southern hemisphere. In addition, Prochlorococcus showed a distinctive depth distribution, with HLI and HLII ecotypes near the surface and co-occurring LL ecotypes further down in the water column. Conversely, Synechococcus generally revealed no obvious depth preference, but did show highly specific distribution at the horizontal scale, with clades I and IV particularly dominating temperate, mesotrophic waters in both the northern and southern hemispheres. The data clearly reveal that specific picocyanobacterial lineages proliferate in similar oceanic provinces separated by large spatial scales. Furthermore, comparison with an earlier AMT dataset suggests that basin scale distribution patterns for Prochlorococcus ecotypes are remarkably reproducible from year to year

    Advisory committee process and program design : low carbon fuel standards

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    Final Report -- Appendix A. Summary of Advisory Committee Input -- Appendix B. Lifecycle Analysis -- Appendix C. Infrastructure Cost Assumptions Memorandum -- Appendix D. Economic Analysis -- Appendix E. Comparable Economic Studies in Other States -- Appendix F. Compliance Scenario Documentation -- Appendix G. Indirect Land Use Change Comparative Analysis -- Appendix H. Fuels Assessment Discussion Paper -- Appendix I. Oregon Biomass Assessment -- Appendix J. Credit and Deficit Calculations -- Appendix K. Review of Biodiesel and Renewable Diesel Use Considerations.principal authors: Sue Langston, David Collier, Cory Ann Wind, Dave Nordberg, Carrie Ann Capp, Wendy Simons.Title from PDF cover (viewed on April 20, 2020)."11-AQ-004."This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references.Mode of access: Internet from the Oregon Government Publications Collection.Text in English
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