1,354,165 research outputs found
SAMDI Mass Spectrometry-Enabled High-Throughput Optimization of a Traceless Petasis Reaction
No full textDevelopment of the self-assembled
monolayer/MALDI mass spectrometry
(SAMDI) platform to enable a high-throughput optimization of a traceless
Petasis reaction is described. More than 1800 unique reactions were
conducted simultaneously on an array of self-assembled monolayers
of alkanethiolates on gold to arrive at optimized conditions, which
were then successfully transferred to the solution phase. The utility
of this reaction was validated by the efficient synthesis of a variety
of di- and trisubstituted allenes
ANALISIS PROFESIONALISME PEGAWAI PT BANK X PADA DIVISI LEGAL COLLATERAL DOCUMENT MANAGEMENT I (LCDM I)
No full textMEIRYANI MUTIARA SAMDI. 2019. 8143165171. Analisis Profesionalisme Pegawai PT Bank X Divisi Legal Collateral Document Management I (LCDM I). Program Studi D3 Administrasi Perkantoran. Fakultas Ekonomi. Universitas Negeri Jakarta.
This paper aims to determine the professionalism of PT Bank X employee in division Legal Collateral Document Management I (LCDM I).The method used in this research is descriptive analysis, with data collection methods through observation and literature.
From writing this paper result can concluded that PT Bank X in division LCDM I has employees who have the ability, education, and expertise are not enough, but it must be accompanied by having a professionalism attitude to work. The non-professionalism of employees at PT Bank X being unprofessional in their attitude while they work or when they are with leader. This results impact to performance of employee in the office not being optimal which the company should be able to achieve.
Key Words : Employee, Professiona
Reactivity of aluminum sulfate and silica in molten alkalimetal sulfates in order to prepare mullite
No full textWith the aim of preparing mullite, reactions between aluminum sulfate and silica in appropriate proportions and molten sulfate media M2SO4 (M=Na and/or K) were performed at different temperatures. The powders obtained were characterized by XRD, FT-IR, SEM and TEM. The reactivity was the same in Na2SO4 and (K,Na)2SO4 media. The best results in terms of yield (98.3%) and weight of mullite produced (95%) were obtained in Na2SO4 at 950 °C. The mullite phase exhibits an acicular morphology (75×0.75 μm) and a specific surface area close to 20 m2/g. In K2SO4 medium, a potassium alumino silicate is formed as well as mullite
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Profiling Protease Activity in Laundry Detergents with Peptide Arrays and SAMDI Mass Spectrometry
No full textLaundry detergent formulations include
proteases to digest and
clean protein-based stains. Methods that can profile protease activity
and specificity in detergents could be important in guiding the development
of engineered proteases for these applications. The work reported
here uses peptide arrays with analysis by self-assembled monolayers
for matrix-assisted laser desorption/ionization (SAMDI) mass spectrometry
to analyze protease activity across a 324-peptide library for five
commercial laundry detergents. The results showed differences in cleavage
activity across different brands. The combination of peptide arrays
and SAMDI mass spectrometry provides a rapid and reliable technique
for analyzing protease activities in laundry detergents and has the
potential to play an important role in the development of new proteases
and detergent formulations
High-Throughput Screening of Small Molecule Libraries using SAMDI Mass Spectrometry
No full textHigh-throughput screening is a common strategy used to identify compounds that modulate biochemical activities, but many approaches depend on cumbersome fluorescent reporters or antibodies and often produce false-positive hits. The development of “label-free” assays addresses many of these limitations, but current approaches still lack the throughput needed for applications in drug discovery. This paper describes a high-throughput, label-free assay that combines self-assembled monolayers with mass spectrometry, in a technique called SAMDI, as a tool for screening libraries of 100 000 compounds in one day. This method is fast, has high discrimination, and is amenable to a broad range of chemical and biological applications
Traceless Immobilization of Analytes for High-Throughput Experiments with SAMDI Mass Spectrometry
No full textLabel-free assays, and particularly
those based on the combination
of mass spectroscopy with surface chemistries, enable high-throughput
experiments of a broad range of reactions. However, these methods
can still require the incorporation of functional groups that allow
immobilization of reactants and products to surfaces prior to analysis.
In this paper, we report a traceless method for attaching molecules
to a self-assembled monolayer for matrix-assisted laser desorption
and ionization (SAMDI) mass spectrometry. This method uses monolayers
that are functionalized with a 3-trifluoromethyl-3-phenyl-diazirine
group that liberates nitrogen when irradiated and gives a carbene
that inserts into a wide range of bonds to covalently immobilize molecules.
Analysis of the monolayer with SAMDI then reveals peaks for each of
the adducts formed from molecules in the sample. This method is applied
to characterize a P450 drug metabolizing enzyme and to monitor a Suzuki–Miyaura
coupling chemical reaction and is important because modification of
the substrates with a functional group would alter their activities.
This method will be important for high-throughput experiments in many
areas, including reaction discovery and optimization
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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