87 research outputs found
Changes in thiol/disulfide homeostasis in patients with chronic kidney disease
No full textThiol/disulfide homeostasis (TDH) is a new marker of oxidative stress. In this study, we would like to determine the changes in TDH in hemodialysis (HD) patients with chronic kidney disease (CKD). This cross-sectional study was conducted in the Nephrology Clinic of Konya Training and Research Hospital. A total of 197 individuals including 75 HD patients, 41 end stage renal disease (ESRD) patients (having stage 3-5 CKD but not receiving hemodialysis), and 81 healthy controls were enrolled in the study. Serum native thiol, total thiol, and disulfide levels were measured with a new method developed by Erel and Neselioglu. It was determined that there was a statistically significant difference in the mean age, body mass index (BMI), modification of diet in renal disease (MDRD), and creatinine level between the three groups (p [Med-Science 2020; 9(1.000): 201-4
Assessment of tumor biomarkers based on a Turkish city hospital's data
No full textBiomarkers can be objectively measured and evaluate the processes of normal biological pathogenic, and pharmacological answers to medications. This study aimed to evaluate the Cancer antigen 15-3 (CA 15-3); Cancer antigen 19-9 (CA 19-9); Cancer antigen 72-4 (CA 72-4); Cancer antigen 125 (CA 125); Carcinoembryonic antigen (CEA); Neuron-specific enolase (NSE), and their test requests in Ankara Bilkent City Hospital. All the results recorded between 01/01/2022 and 31/12/2022 were acquired from our hospital laboratory information system.136,043 tests, consisting of 28,132 CA 15-3, 37,543 CA 19-9, 3135 CA 72-4, 29,996 CA125, 36,788 CEA, and 449 NSE tests, were enrolled. The frequency of positive tumor markers were as follows: CA 15-3 U/mL (N=996, 3.5%), CA 19-9 U/mL (N=4716,12.6%), CA 72-4 U/mL (N=174, 5.6 %), CA125 U/mL (N=3488, 11.6%), CEA ng/mL (N=1699, 4.6%), NSE IU/ mL (N=396, 88.2%). Statistical differences in CA 15-3 U/mL(p [Med-Science 2025; 14(3.000): 864-9
Thiol/disulfide homeostasis in patients with ankylosing spondylitis
No full textAnkylosing spondylitis (AS) is a chronic inflammatory disease. In many inflammatory diseases, increased production of pro-inflammatory cytokines is associated with an increase in oxidative stress mediators. Thiol/disulfide homeostasis is a marker for oxidative stress. The aim of this study was to examine the dynamic thiol/disulfide homeostasis in AS. Sixty-nine patients with AS and 60 age- and sex-matched controls were included in the study. The Bath Ankylosing Spondylitis Disease Activity Index (BASDAI) and visual analogue scale (VAS) were used to determine the disease activity. Native thiol, total thiol, and disulfide levels were measured with a novel automated method recently described by Erel and Neselioglu. The aforementioned method is also optionally manual spectrophotometric assay. The total thiol levels were significantly lower in the AS group compared with the control group (p = 0.03). When the patients were divided into active (n = 35) and inactive (n = 34) subgroups using BASDAI scores, the native plasma thiol and total thiol levels were significantly lower in the active AS patients compared to the inactive AS patients (p = 0.02, p = 0.03 respectively). There was a negative correlation between the plasma native thiol levels and VAS, BASDAI scores. Thiol/disulfide homeostasis may be used for elucidating the effects of oxidative stress in AS. Understanding the role of thiol/disulfide homeostasis in AS might provide new therapeutic intervention strategies for patients
An Investigation of Oxidative Stress and Thiol/Disulphide Homeostasis in Graves’ Disease
No full textBackground and objectives: The aim of this study was to research oxidative stress and thiol/disulphide homeostasis in Graves’ patients. Materials and Methods: The study included 33 Graves’ patients (research group) and 35 healthy subjects (control group). Serum oxidative stress and thiol/disulphide homeostasis (a new and automated spectrophotometric method developed by Erel and Neselioglu) parameters were studied and compared between the groups. Results: The native and total thiol levels and the native thiol/total thiol ratio were lower in patients with Graves’ disease compared to the control group (p < 0.001, p < 0.001, and p = 0.006, respectively). TOS (total antioxidant status), PC (protein carbonyl), OSI (Oxidative stress index), and disulphide/native thiol and disulphide/total thiol ratios were determined to be higher in the Graves’ disease group than in the control group (p < 0.001, p = 0.001, p = 0.001, p = 0.004, and p = 0.006, respectively). In the Graves’ disease group, the free triiodothyronine (FT3) and free thyroxine (FT4) levels were significantly positively correlated with impaired thiol/disulphide homeostasis and oxidative stress parameters (p < 0.05). Conclusion: The results of the current study demonstrated that oxidative stress and thiol/disulphide homeostasis increased towards disulphide formation due to thiol oxidation in Graves’ disease. In addition, a positive correlation of FT3 and FT4 was observed with oxidative stress parameters and impaired thiol/disulphide homeostasis
Thiol/disulfide homeostasis in patients with telogen effluvium: is oxidative stress important in the pathogenesis of telogen effluvium?
No full textObjective: The aim of this study was to assess the correlation between telogen effluvium (TE) with the new oxidative stress (OS) indicator of thiol/disulfide balance and to research the role of OS in the pathogenesis of TE. Methods: Our study included 101 patients with TE diagnosis and 39 healthy individuals. Serum thiol/disulfide was measured with a new automated spectrometric method developed by Erel and Neselioglu, and results were compared statistically. Results: Among the six thiol/disulfide parameters, there were statistically significant differences for native thiol, total thiol, disulfide, disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol studied in the patient and control groups (P = 0.042, 0.044, < 0.001, respectively). Conclusions: Based on the results of this study, it can be said that OS is closely associated with TE pathogenesis. There is a need for new studies that will show the possible effects of OS on TE pathogenesis and research different OS markers in addition to thiol/disulfide parameters
Pediatric Carbon Monoxide Poisoning Effects of Hyperbaric Oxygen Therapy on Thiol/Disulfide Balance
No full textObjectives Carbon monoxide (CO) poisoning remains the foremost cause of poisoning worldwide. This study aimed to investigate the effects of hyperbaric oxygen therapy (HBOT) and normobaric oxygen therapy (NBOT) on thiol/disulfide homeostasis in children with CO intoxication. Methods Eighty-one children aged 0 to 18 years with CO intoxication were included in this cross-sectional study. No changes were made in the routine clinical evaluation and treatment practices of the patients. Thirty-two children who received HBOT and 49 children who received NBOT were compared for serum native thiol, disulfide, and total thiol levels, as well as for the changes in disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol ratios before and after treatment. Results Antioxidant levels, such as native thiol and total thiol, were significantly decreased in patients who received HBOT and increased in those who received NBOT (P = 0.02 and P = 0.01, respectively). There was no statistically significant difference between the 2 groups concerning the change of native thiol/total thiol ratios (P = 0.07). In addition, there was no significant difference regarding changes in disulfide, disulfide/native thiol, and disulfide/total thiol levels before and after treatment (P = 0.39, P = 0.07, and P = 0.07, respectively). Conclusions Although thiol-disulfide balance is maintained in patients treated with HBOT, antioxidant levels decrease significantly compared with NBOT. Despite efficiency of HBOT in CO intoxication, oxidative stress and reperfusion injury due to hyperoxygenation should be considered in the treatment of HBOT
Development of a New Colorimetric, Kinetic and Automated Ceruloplasmin Ferroxidase Activity Measurement Method
No full textBackground: Ceruloplasmin plays an important role in the regulation of iron metabolism. Ceruloplasmin is an acute-phase protein known to have many metabolic effects. Its activity increases during infection, inflammation, and compensation of oxidation. In the current study, our aim is to develop a new method for the measurement of ferroxidase activity without requiring any chromogen. Methods: Venous blood samples were collected into serum separator tubes. Ferric iron ions formed by the enzyme ferroxidase were measured, both manually and fully automatically, at the 415 nm wavelength without using chromogen. These results were compared to conventional ferroxidase measurement methods and to the immunoturbidimetric ceruloplasmin measurement method. Results: The detection limit of the new assay was 14.8 U/L. The upper limit of the linearity was 1380 U/L. Precision values were calculated for high, medium, and low levels of ferroxidase activity in serum pool. The coefficient of variation was <5% for each level. Conclusion: In the present method, chromogens are not used. With its considerably low cost and short reaction time, this method is able to provide fast results, can be performed easily, and makes accurate measurements
The relationship between thiol-disulfide balance and prostate cancer
No full textThis study aimed to investigatethe possible association of thiol/disulfidehomeostasiswith prostate-specific antigen levelsin prostate cancer patients and to compare the results with a normal healthy population ofa similar age group for the first time in literature. Forty-twopatients (20 patientswith prostate cancer and 22 volunteers) were included in the study. Thiol/disulfide homeostasis tests were measured with an automated method. Albumin, carcinoembryonic antigen (CEA), prostate-specific antigen (PSA), ischemia modified albumin (IMA), total thiol (TT), native thiol (SH), and disulfide (SS) levelsand, thiol-disulfide ratios (disulfide / native thiol, disulfide/total thiol and native thiol/total thiol) were assessed to detect any differences between prostate cancer group and control group. The mean PSA value of the prostate cancer group was 2.56 ng/mL, the mean PSA value of the control group was 1.21 ng/mL, and the mean age of the prostate cancer group was 67.32 years, the mean age of control group was 60.09. Although native thiol and total thiol levels were significantly lower in patients with prostate cancer (p 0.05). In our study, we could not show thiol-disulfide values as a biochemical prognostic factor in patients with prostate cancer. We believe that serum TT, SH, SS concentrations cannot serve as a noninvasive biomarker for prostate cancer. To verify the biochemical role of thiol/disulfide balance, studies need to be done by increasing the number of patients with prostate cancer. [Med-Science 2020; 9(3.000): 779-83
Serum ischemia modified albumin and dynamic thiol/disulfide homeostasis in early- and late-onset preeclampsia
No full textAim: In the present study, we aimed to evaluate serum IMA, IMA/albumin ratio, and DTDH levels in patients with early- and late-onset PE compared to healthy controls.Impaired homeostasis between oxidant and antioxidant mechanisms, inflammatory processes, and endothelial dysfunction play a key role in the pathogenesis of preeclampsia (PE). Serum ischemia modified albumin (IMA) and dynamic thiol/disulfide homeostasis (DTDH) levels are elevated in the presence of inflammation, oxidative stress, and endothelial dysfunction. Material and Methods: A total of 24 patients with early-onset PE and 62 patients with late-onset PE were included.The control group consisted of 46 healthy controls with similar gestational weeks. Serum samples were collected and IMA, albumin, and native, total, and disulfide thiol levels analyzed. Corrected IMA/albumin ratios were also calculated.Results: Disulfide levels, disulfide/native and disulfide/total thiol levels were higher in patients with early-onset PE compared to late-onset patients(p=0.008, p=0.022, and p=0.021, respectively). However, there was no significant difference between the late-onset PE patients and late-onset PE controls. Although there was no significant difference in the IMA levels between the patient and control groups, the IMA/albumin ratio was higher in the early-onset and late-onset PE patients, compared to the control group. However, there was no significant difference between the early-onset and late-onset PE patients.Conclusion: Our study results showed increased disulfide levels, disulfide/native thiol, disulfide/total thiol and IMA/albumin ratio in the early-onset PE patients, indicating increased oxidative stress in the pathogenesis of PE. In the late-onset PE patients, there was an increase only in the IMA/albumin ratio. However, further large-scale, prospective studies are needed to confirm the diagnostic value of these markers in the clinical practice
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