60,987 research outputs found
A distributed amplifier system for bilayer lipid membrane (BLM) arrays with noise and individual offset cancellation
No full textLipid bilayer membrane (BLM) arrays are required for high throughput analysis, for example drug screening or advanced DNA sequencing. Complex microfluidic devices are being developed but these are restricted in terms of array size and structure or have integrated electronic sensing with limited noise performance. We present a compact and scalable multichannel electrophysiology platform based on a hybrid approach that combines integrated state-of-the-art microelectronics with low-cost disposable fluidics providing a platform for high-quality parallel single ion channel recording. Specifically, we have developed a new integrated circuit amplifier based on a novel noise cancellation scheme that eliminates flicker noise derived from devices under test and amplifiers. The system is demonstrated through the simultaneous recording of ion channel activity from eight bilayer membranes. The platform is scalable and could be extended to much larger array sizes, limited only by electronic data decimation and communication capabilities
PIWULANG MORAL ING LAMPAHAN RINGGIT “LAMPAHAN BRATAYUDA-III RANJAPAN PEJAHIPUN ANGKAWIJAYA ANGGITANIPUN KAMAJAYA SAHA U. J. KATIJO WP.”
No full textAncasing panaliten inggih menika: (1) ngandharaken wujuding piwulang moral, (2) ngandharaken jinising piwulang moral, saha (3) ngandharaken caranipun ngandharaken piwulang moral wonten ing lampahan ringgit kanthi irahirahan “Lampahan Bratayuda-III Ranjapan Pejahipun Angkawijaya Anggitanipun Kamajaya saha U. J. Katijo Wp.” Panaliten menika kanthi metode deskripsi tumrap piwulang moral-ipun. Data ing panaliten menika awujud tembung, frasa, ukara, saha paragraf. Dene sumber data-nipun inggih menika lampahan ringgit kanthi irah-irahan “Lampahan Bratayuda-III Ranjapan Pejahipun Angkawijaya Anggitanipun Kamajaya saha U. J. Katijo Wp.”. Data kakempalaken kanthi migunakaken teknik maos saha cathet. Kanthi pambiyantuning kartu data, data dipun-klasifikasi-kaken dados 3 werni inggih menika: wujuding piwulang moral, jinising piwulang moral, saha cara ngandharaken piwulang moral, sarta dipun-analisis kanthi deskriptif. Pengesahan data panaliten kanthi ngginakaken validitas semantis saha reliabilitas pengamatan ulang utawi stabilitas (intrarater). Salajengipun, kanthi panaliten menika kapanggihaken: (1) wujud piwulang moral gayutanipun manungsa kaliyan Gusti Allah, antawisipun pitados dhateng pepesthen, donga panyuwunan, sukur, pasrah, mituhu, eling, tansah kaluberan berkah, (2) wujud piwulang moral gayutanipun manungsa kaliyan sasami manungsa, antawisipun boten kenging srakah, sagah ambelani kulawarga, bangsa, lan nagari, enget kabecikanipun tiyang sanes, ana rembug becik dirembug, tut wuri handayani, kedah gadhah unggah-ungguh, kedah siyaga, sendika ing dhawuh, aja adigang adigung adiguna, gumatos, kedah imbang, tanggap ing sasmita, betah pambiyantuning sanes, boten kenging curang, aja mung melik gebyar, angon mangsa, tresna asih, boten waton ngendika, kedah paring pitutur, tepa slira, (3) wujud piwulang moral gayutanipun manungsa kaliyan alam, antawisipun boten kenging ngrisak alam, saha (4) gayutanipun manungsa kaliyan dhiri piyambak, antawisipun kedah tatag, boten kenging sumelang & sedih, ayem tentrem, mawas dhiri, waskitha, manteb, trengginas, kendel, kedah sabar sareh, boten kenging cupet ing nalar, waspada, kedah gadhah gegayuhan, titen. Dene cara ngandharaken piwulang moral ing panaliten menika kanthi cara langsung saha cara boten langsung
EVALUATION OF HISTONE DEACETYLASE INHIBITOR EFFECTS ON THYROID CANCER
No full textAbstract
Histone deacetylases (HDACs) play a crucial role in the proper regulation of cellular functions through their connection with chromatin and transcriptional regulation. Alterations in HDAC activity have been reported in several types of cancer encouraging development of HDAC inhibitors (HDACis) for cancer treatment. The antitumor activity of HDACi has been demonstrated, in clinical trials, in both solid and non solid neoplasias at doses well tolerated by patients. However, the molecular basis for their tumor selectivity is unknown. Anaplastic thyroid carcinoma (ATC) is one of the most aggressive malignancies, having a poor prognosis and being refractory to conventional chemo- and radiotherapy. To the aim to find an innovative therapy for the treatment of ATCs, we studied the effects of two potent HDACis, SAHA and MS-275, on rat thyroid cell lines transformed by the v-ras-Ki oncogene which is frequently mutated in ATCs. We show that: i) HDAC 1 and HDAC 2 are overexpressed in anaplastic thyroid carcinomas compared to normal thyroid; ii) SAHA and MS-275 induce apoptosis selectively in completely transformed rat thyroid cells; iii) TNF-related apoptosis-inducing ligand (TRAIL) is the main mediator of cell death induced by SAHA; iv) SAHA stabilize TRAIL protein by affecting its proteasome-mediated degradation
Insight on the presence of dimethylammonium cation within anionic metal-organic supramolecular host: structural, Hirshfeld surface, optical and theoretical analysis
No full textA new supramolecular metal-organic complex, [(DMA)(+){Fe(IDA)(2)}(-)] (where H(2)IDA = iminodiacetic acid and DMA = dimethylammonium cation) (1), has been synthesized solvothermally at 90 degrees C from reaction of ferric(III) chloride hexahydrate with nitrilotriacetic acid (H(3)NTA) in stoichiometric ratio. The complex was characterized by single crystal X-ray diffraction (SCXRD) along with spectroscopic analyses. During the reaction process, H(3)NTA is degraded into IDA(2-) ligand. Structural analysis reveals that 1 is mononuclear and crystallizes in the monoclinic space group C2/c. Within the [Fe(IDA)(2)](-) unit, Fe3+ shows six-coordinate distorted octahedral geometry. The [Fe(IDA)(2)](-) units are connected by strong N1-H1 center dot center dot center dot O4 hydrogen bonds to form anionic 2D supramolecular layers which are further connected by weak C-H center dot center dot center dot O hydrogen bonds to form a 3D metal-organic supramolecular host (MOSH) structure having 1D supramolecular channels along the crystallographic c-axis. DMA cations are present within the supramolecular channels through N2-H2 center dot center dot center dot O2 hydrogen bonding interactions. Hirshfeld surface analysis and corresponding 2D fingerprint plots indicate that O center dot center dot center dot H interactions are the major supramolecular interactions present between MOSH and guest DMA cation. DFT calculations reveal that the HOMO and LUMO of the [Fe(IDA)(2)](-) unit are composed of mixed metal-ligand orbitals. Both the absorption and emission spectra of the complex were studied in aqueous phase and the result was correlated with the TDDFT study
A Dynamic Subfilter-scale Stress Model for Large Eddy Simulations Based on Physical Flow Scales
No full textWe propose a new definition of the length scale in an eddy-viscosity model for large-eddy simulations (LES). This formulation extends and generalizes a previous proposal [Piomelli, Rouhi and Geurts, Proc. ETMM10, 2014], in which the LES length scale was expressed in terms of the integral length-scale of turbulence determined by the flow characteristics and explicitly decoupled from the simulation grid; this approach was named Integral Length-Scale Approximation (ILSA). As in the original ILSA, the model coefficient was determined by the user, and required to maintain a desired contribution of the unresolved, subfilter scales (SFS) to the global transport. We propose a local formulation (local ILSA) in which the model coefficient is local in space, allowing a precise control over SFS activity as a function of location. This new formulation preserves the properties of the global model; application to channel flow and backward-facing step verifies its features and accuracy
Large-eddy simulation of a separated flow with a sub-filter scale model based on the integral length-scale
No full textA new sub-filter scale model for large-eddy simulations, which uses a length-scale proportional to the integral scale of the turbulence instead of the grid resolution to parametrize the modelled stresses, will be assessed in the prediction of the flow of a boundary-layer over a rough surface, which includes separation and reattachment
Near Wall PIV-Measurements on the Windward Slope of a Hill
No full textThe turbulent flow over periodic hills was measured near to the wall, using planar Particle-Image-Velocimetry (PIV) at high spatial resolution. Our focus is on the near wall turbulence structure on the windward slope of the hill. For large-eddy simulation (LES) we suspect that, if this was not predicted accurately, it affects the prediction of the velocity profiles over the hill crest which in turn will affect the recirculation length downstream of the hill. Regarding the time averaged velocities, we were able to resolve the linear viscous region of the boundary layer. The velocity distribution and also the Reynolds stress does not comply with the law of the wall as it is valid for a turbulent boundary layer at equilibrium
Energy dissipation and flux laws for unsteady turbulence
No full textDirect Numerical Simulations of spatially periodic unsteady turbulence show that the high Reynolds number scalings of the instantaneous energy dissipation rate and interscale energy flux at intermediate wavenumbers are qualitatively different from the well-known cornerstone scalings of equilibrium turbulence where and are time-dependent rms velocity and integral length-scales. Instead, they both scale as where and are length and velocity scales characterizing initial/overall unsteady turbulence conditions
Direct numerical simulation of turbulent Couette-Poiseuille flow with zero skin friction
No full textThe near-wall scaling of mean velocity U(y) is addressed for the case of zero skin friction on one wall of a fully turbulent channel flow. The present DNS results can be added to the evidence in support of the conjecture that U is proportional to √yw in the region just above the wall at which the mean shear dU/dy = 0
Real-space Manifestations of Bottlenecks in Turbulence Spectra
No full textAn energy-spectrum bottleneck, a bump in the turbulence spectrum between the inertial and dissipation ranges, is shown to occur in the non-turbulent, one-dimensional, hyperviscous Burgers equation and found to be the Fourier-space signature of oscillations in the real-space velocity, which are explained by boundary-layer-expansion techniques. Pseudospectral simulations are used to show that such oscillations occur in velocity correlation functions in one- and three-dimensional hyperviscous hydrodynamical equations that display genuine turbulence
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