36 research outputs found
Coleus amboinicus extract increases transforming growth factor-1β expression in Wistar rats with cisplatin-induced nephropathy
Get PDFBackground and Aim: Coleus amboinicus (CA) plants are known to exert antibacterial and anti-inflammatory effects and demonstrate antiproliferative effects against cancer cells. This study aimed to investigate the activity of CA extract on the expression of transforming growth factor-1β (TGF-1β) in cisplatin-induced nephropathy in Wistar rats (Rattus norvegicus).
Materials and Methods: CA was obtained from fresh leaves of CA and was extracted using 96% ethanol maceration. This blinded, controlled, randomized post-test study assigned 24 Wistar rats to three groups (n=8). Negative controls received normal saline (P0), nephropathy was induced in rats by cisplatin (5 mg/kg, IP) (P1), and treated with ethanolic coleus extract (500 mg/kg, PO) (P2), respectively, for 7 days. Nephropathy was induced on the 4th day. All rats were sacrificed on the 8th day for blood and kidney sample collection. Concentrations of blood urea nitrogen (BUN), creatinine, and alkaline phosphatase were analyzed using colorimetric analysis. A semi-quantitative analysis was performed on sectioned kidneys to determine the numbers of positive cells for TGF-1β expression and to evaluate structural and functional alterations in the kidneys using histopathological and immunohistochemical staining.
Results: The concentrations of BUN, creatinine, and alkaline phosphatase from blood samples in the treatment group were significantly lower than those of the control group (p<0.05). Morphological evaluation of the tubular interstitium and glomeruli revealed that necrotic, degenerating, and infiltration of cells significantly decreased in the treatment group compared to the control group (p<0.05). The mean immunostaining scores indicating the presence of TGF-1β were 7.8 in the ethanolic coleus extract group, 3 in the induction group, and 2.3 in the control group. The expression scores for TGF-β1 were significantly different between the ethanolic coleus extract treatment and control group (p<0.05).
Conclusion: Our results suggest that in Wistar rats with cisplatin-induced nephropathy, CA extract inhibits pathological lesions by regulating the renal expression of TGF-1β in areas containing the renal tubules and glomeruli
MEKANISME KERJA EKSTRAK ETANOL BATANG TORBANGUN (Coleus amboinicus) TERHADAP MODEL NEFROPATI AKIBAT INDUKSI ASAM URAT PADA TIKUS PUTIH (Rattus norvegicus)
Get PDFIntroduction. Torbangun (Coleus amboinicus, CA) extracts are known to have
anti-oxidant activity, anti-platelet aggregations, anti-bacterial, anticancer and antiinflammation.
Aim. The purposed to study the mechanism of Coleus amboinicus (CA) stem
extracts in Wistar rats with uric acid-oxonic acid induced nephropathy, by
analyzing the levels of Glutathione Peroxidase (GPx) activity, Interleukin-1β (IL-
1β), Transforming growth factor-β1 (TGF-β1) expression and macrophage CD 68
(ED-1).
Method. Fifty-six male Wistar rats (Rattus norvegicus), 3 months with 150-200 g
body weight, were allocated into four groups (n=14). The control group received
placebo, treatment group were administered orally with uric acid 1,5% and oxonic
acid 2% (P1) and received 500 mg/kg BW of the CA stem extracts (P2) and
received 5 mg/kg BW quercetin (P3) by oral, respectively for 35 days. Blood
serums collected for analysis of cystatin C (Cys-C), creatinine and BUN
concentrations. All groups were sacrificed to collect kidney organ for measuring
of GPx activity, IL-1β expression, TGF-β1 expression was conducted by Avidin-
Horseradish Peroxidase (HRP) Sandwich-ELISA. Kidney organ was collected to
histopathological analyzed by Hematoxylin-eosin and Periodic Acid Schiff
staining. Immunohistochemistry is conducted for identification of CD 68
expression. The levels of GPx, IL-1β, TGF-β1, CD 68 and Cys-C concentrations
were analyzed by statistic independent test.
Results. Coleus amboinicus (CA) stem extract by Thin Layer Chromatography
has a relative fraction of flavonoids, terpenes, saponins, polyphenols and
alkaloids. By in silico analyzed, quercetin as a marker compound of CA stem
extract has stronger bind to the TGF-β1receptor (PDB code: 4X0M) than its of
3WA_601 ligand. Induction with uric acid- oxonic acid for 35 days has proven to
causes nephropathy with indicated of elevated BUN, creatinine and cystatin-C
levels and necrotic lesions of tubular membrane and glomerular kidney, in P1, P2,
and P3 groups. The research results showed that CA stem extract at a dose of 500
mg/kg BW decreased the activity of GPx, IL-1β expressions and CD 68 not
significantly (p> 0.05) and increased of TGF-β1 expression significantly (p≤0.05)
in P2 and P3 groups than the P1 group. This study was concluded that torbangun
stem extract is proven to inhibit on uric acid-induced nephropathy, however, a
mechanism of that inhibitory activity could not explain clearly in this research
Effect of Piper crocatum Extract on Kidney Histopathology in Alloxan-Induced Wistar White Rats (Rattus norvegicus)
No full textThe prevalence of diabetes mellitus is steadily increasing. According to the International Diabetes Federation in 2021, the number of diabetes sufferers in Indonesia has rapidly increased in the last ten years. This study aims to determine the effect of red betel leaf extract (Piper crocatum) on kidney histopathology of Wistar white rats (Rattus norvegicus) induced by alloxan. A total of 25 white male Wistar rats aged ± 3 months were used in this study. All samples were divided randomly into five treatment groups, namely: (K-) healthy rats that were only given distilled water; (K+) given a single dose of alloxan 120 mg/kg bw/intraperitoneal; (P1) rats given alloxan 120 mg/kgbw/intraperitoneally and red betel leaf extract 100 mg/kg bw/orally; (P2) rats given alloxan 120 mg/kg bw/intraperitoneally and red betel leaf extract 150 mg/kg bw/orally; (P3) rats were given alloxan 120 mg/kgbw/intraperitoneally and red betel leaf extract 200 mg/kg bw/orally. Treatment was given for 14 days. Blood glucose levels were measured using the Glucometer (EZ Smart) colorimeter method. On the day 15th, all rat were euthanized and necropsied to remove the kidneys and then made into preparations with HE staining. The histopathological lesions observed were necrosis, degeneration, and inflammatory cell infiltration. Histopathological observations were carried out using a microscope with 40x magnification. Data resulting from observations were analyzed using SPSS 20 with Kruskal-Wallis and Mann-Whitney (P≤0.05). It revealed that red betel leaf extract has a potential ability to reduce the effects of hyperglycemia, and by inducing 2% of red betel leaf extract at dose of 100 mg/kg bw, 150 mg/kg bw, and 200 mg/kg bw could reduce necrosis, inflammatory cell infiltration, and degeneration significantly in the kidneys of white rats
Efek anti radang ekstrak torbangun (Coleus amboinicus) pada induksi cisplatin tikus putih wistar
Get PDFPenelitian ini bertujuan menganalisis aktivitas ekstrak torbangun (Coleus amboinicus) terhadap biokimia darah dan gambaran histopatologi ginjal pada induksi cisplatin pada tikus putih Wistar (Rattus norvegicus). Penelitian ini menggunakan 24 tikus putih galur Wistar yang terbagi menjadi 3 kelompok yaitu kelompok kontrol negatif dengan pemberian plasebo (P0), kelompok kontrol positif berupa induksi cisplatin dengan pemberian CMC-Na 0.1 % (P1), dan kelompok induksi cisplatin dengan pemberian ekstrak torbangun 500 mg/ kg bb (P2). Pembuatan nefropati pada kelompok perlakuan dengan injeksi intra peritoneal hari ke-4 dosis tunggal cisplatin 5 mg/kg bb. Seluruh kelompok tikus dikorbankan dan diambil sampel darah untuk diukur kadar blood urea nitrogen (BUN), serum kreatinin (SK) dan alkalin phospatase (ALP) sebagai tes fungsi ginjal. Sampel organ fiksasi dengan BNF 10 % untuk histopatologi. Hasil menunjukkan bahwa pemberian ekstrak Coleus amboinicus menurunkan konsentrasi BUN, serum kreatinin, dan alkalin phospatase pada kelompok dengan ekstrak torbangun (P2) dibandingkan dengan kelompok kontrol positif (p<0,05). Analisis histopatologi terlihat perdarahan multifokal, infiltrasi interstisial, adanya adesi membrane capsula glomerulus dan nekrotik tubular secara difus pada kelompok kontrol positif dengan induksi cisplatin (P1). Kerusakan pada glomerulus dan tubulus ginjal lebih ringan pada kelompok yang diberi eksrak torbangun (P2). Berdasarkan hasil penelitian dapat disimpulkan pemberian ekstrak torbangun dapat menormalkan gambaran biokimia darah dan menghambat kerusakan ginjal dengan cara menghambat keradangan pada induksi cisplatin pada tikus putih
Ethical document
No full textDocument Form for Ethical Testing on Experimental Animals Conducted by the Animal Ethics Committee of the Faculty of Veterinary Medicine, Wijaya Kusuma University, Surabaya</p
Uji Antipiretik Patch Ekstrak Etanol Alang-Alang dengan Enhancer SPAN-80 terhadapTemperatur dan Jumlah Neutrofil pada Tikus Putih
Get PDFAlang-alang dikenal sebagai tanaman pengganggu, namun alang-alang memiliki banyak manfaat di bidang kesehatan, salah satunya sebagai penurun demam karena alang-alang mengandung flavonoid. Flavonoid bekerja menghambat enzim siklooksigenase yang bertanggung jawab atas produksi mediator inflamasi sehingga suhu tubuh akan kembali menjadi normal. Alang-alang diformulasikan dalam bentuk sediaan patch dengan penambahan enhancer Span-80 sebagai peningkat penetrasi. Tujuan dari penelitian ini adalah untuk menganalisis efektivitas sediaan patch ekstrak etanol Imperata cylindrica dengan dan tanpa penambahan enhancer Span-80 terhadap temperatur dan jumlah neutrofil tikus putih yang diinduksi vaksin DPT. Hewan perlakuan sebanyak 30 ekor tikus yang dibagi secara acak menjadi 5 kelompok perlakuan yaitu P0 (pemberian makan dan minum), P1 (parasetamol sirup), P2 (patch tanpa ekstrak etanol alang-alang), P3 (patch ekstrak etanol alang-alang tanpa enhancer), dan P4 (patch ekstrak etanol alang-alang dengan enhancer). Pada penelitian ini, dilakukan pengamatan temperatur awal tubuh tikus, setelah diinduksi vaksin DPT pada menit ke-15, 30, 45, 60, 120, 180, 240, 300 dan jumlah neutrofil pada menit ke-0, 30, 60, 120, 180, 240, 300. Hasil penelitian menunjukkan bahwa patch ekstrak etanol alangalang dapat menurunkan temperatur tubuh namun penurunan jumlah neutrofil tikus putih tidak berbeda bermakna antara patch ekstrak etanol alang-alang dengan atau tanpa enhancer
Study of Thitonia Diversifolia Extract in Histomorphology of Pancreas and Interleukin-1beta expression on Aloxan Induced Wistar Rats
Get PDFThe purpose of this study is to determine effect of antiinflammation of Thitonia diversifolia (TD) extracts in histopathology liver and pancretic tissue on alloxan-induced in Wistar rat. Thirty two male Wistar rats (Rattus norvegicus) with a body weight (bw)150-200 g in 3 month of age, were allocated into four groups, with eight animals per group. The control group received normal saline (P0), positive control group received CMC Na 0.01% (P1), the treatment group were received 100 mg/kg bw of the TD extracts (P2) and received 100 mg/kg bw of the metformin (P3) respectively for 7 days. Bloods serum collected for measuring of blood glucose (BG). cholesterol and insulin (INS) concentrations. The levels of insulin (INS) concentrations were analysed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. All animal were sacrified for tissue staining with haematoxylin eosin. Thitonia diversifolia extract significantly decreased the level of blood glucoseand choelsterol concentrations compared to the postive control group (p<0.05). The level of INS could be detected by its level significantly increased in TD treatment group (p<0.05). Histopathological showed that induction of alloxan could effect severe multifocal degeneration, vacuolisation, cell inflammation, acute langerhan cells injury with destruction cells. Immunohistochemical staining labeled with IL-1β monoclonal antibodies (Mab) showed lowering expression of IL-1β reveal as brownish color aggregates on the langerhans cells in the TD treatment group. This study was concluded that TD extract is inhibited hepatic tissue injuries and decreased destruction in langerhan cells by decreasing IL-1β expression on alloxan-induced rats
Insiden Hewan Qurban sebagai Vektor Penular Penyakit Cacing Hati (Fasciolosis) di Surabaya
Get PDFQurban animals slaughtering in ied qurban commonly increases the demand of livestock and thusautomatically escalate the livestock traffic. as well as the level of prevention against transmission of diseases. The aim of this research is to determine the possibility of qurban animals as vector of fasciolosis transmission in Surabaya through the prevalence of fasciolosis among qurban animals and the magnitude of the economic losses due to the damage of the liver. Research was performed during ied qurban 1436h on september 24th 2015 upon a total Samples of 384 qurban animals. Fasciolosis was taken as a diagnoseBased on the findings of fasciolain liver during inspection and their eggs in the faeces upon laboratory examination. overall, the prevalence of fasciolosis among all types of qurban animals in Surabaya in 2015 based on liver inspection was 18,5%, meanwhile fecal examination was 15,1%. In detail, qurban cattle showed 28,1% and 24.6%, whereas goats revealed 10,8% and 7.5% prevalences of fasciolosis due to the findings of fasciola on liver inspection and eggs on faeces examination respectively. Economic losses due to liver damage was calculated around Rp. 6.962.362.500,-
Study of doking molecule flavonoid Coleus amboinicus in TGF-1b receptor and lowering MDA concentration on Cisplatin-induce Wistar Rats
No full textThe aims of this study is to evaluate molecular docking of flavonoid Coleus amboinicus (CA) extracts in transforming growth factor-1b and lowering MDA concentration on cisplatin-induced in Wistar rat. Eighteen male Wistar rats (Rattus norvegicus), 3 months of age with a body weight (BW) of 150-200 g, were allocated into three groups, with six animals per group. The control group received aquadest (P0), the treatment group were treated with single doses of cisplatin (5 mg/kg bw., ip) (P1) and received 100 mg/kg bw of the CA extracts (P2) respectively for 7 days. Bloods collected for analysis of serum alkaline phospatase (AP), Blood Nitrogen Urea (BUN) and Malondialdehid (MDA) concentrations. The levels of Malondialdehid (MDA) concentrations were analysed by Avidin-Horseradish Peroxidase (HRP) Sandwich-ELISA. All groups were sacrified for histopathology. Coleus amboinicus extract significantly decreased the level of AP, BUN and MDA concentrations compared to the control group (p<0.05). The level of MDA could be detected by its level significantly decreased in CA treatment group (p<0.05). Coleus amboinicus (CA) extract has a flavonoid as a marker compound of CA extract has stronger bind to the TGF-β1receptor than its of 3WA_601 ligand in silico analyzed. In histopathological examination showed that cisplatin-induced could alter severe multifocal hemorrhage, interstitial congestion, cell inflammatory, acute glomerular and tubular injury with necrotic cells. Immunohistochemical staining labeled with TGF-1β monoclonal antibodies (Mab) showed marked expression of brownish color aggregates on the surface of tubular epithelial cells and around glomerular mesangial cells in the CA treatment group. This study was concluded that CA extract is inhibited renal tissue injuries by lowering MDA and increasing TGF-1b expression on cisplatin-induced rats. Flavonoid as marker of CA extract has stronger bind to TGF-1b receptor by in silico
White blood cell differential count
No full textWhite blood cell differential count result in diabetic-induced rat with treatment by zinc oxide Tithonia diversifolia nanoparticle emulsion</p
