8,723 research outputs found
The acidic cluster of the CK2 site of the cation-dependent mannose 6-phosphate receptor (CD-MPR) but not its phosphorylation is required for GGA1 and AP-1 binding
No full textLysosomal biogenesis depends on proper transport of lysosomal enzymes by the cation-dependent mannose 6-phosphate receptor (CD-MPR) from the trans-Golgi network (TGN) to endosomes. Trafficking of the CD-MPR is mediated by sorting signals in its cytoplasmic tail. GGA1 (Golgi-localizing, gamma-ear-containing, ARF-binding protein-1) binds to CD-MPR in the TGN and targets the receptor to clathrin-coated pits for transport from the TGN to endosomes. The motif of the CD-MPR that interacts with GGA1 was shown to be (DXXLL65)-D-61. Reports on increased affinity of cargo, when phosphorylated by casein kinase 2 (CK2), to GGAs focused our interest on the effect of the CD-MPR CK2 site on binding to GGA1. Here we demonstrate that Glu(58) and Glu(59) of the CK2 site are essential for high affinity GGA1 binding in vitro, whereas the phosphorylation of Ser(57) of the CD-MPR has no influence on receptor binding to GGA1. Furthermore, the in vivo interaction between GGA1 and CD-MPR was abolished only when all residues involved in GGA1 binding were mutated, namely, Glu(58), Glu(59), Asp(61), Leu(64), and Leu(65). In contrast, the binding of adaptor protein-1 (AP-1) to CD-MPR required all the glutamates surrounding the phosphorylation site, namely, Glu(55), Glu(56), Glu(58), and Glu(59), but like GGA1 binding, was independent of the phosphorylation of Ser(57). The binding affinity of GGA1 to the CD-MPR was found to be 2.4-fold higher than that of AP-1. This could regulate the binding of the two proteins to the partly overlapping sorting signals, allowing AP-1 binding to the CD-MPR only when GGA1 is released upon autoinhibition by phosphorylation
Jonathan Ned Katz Author Event: The Daring Life and Dangerous Times of Eve Adam
No full text“The Daring Life and Dangerous Times of Eve Adams,” interview with author, Jonathan Ned Katz, moderated by Emily Weiner (WWU) and organized by Congregation Beth Israel
The problematic syndrome of right temporal lobe atrophy: Unweaving the phenotypic rainbow
No full textChristopher R. S. Belder, Anthipa Chokesuwattanaskul, Charles R. Marshall, Chris J. D. Hardy, Jonathan D. Rohrer, and Jason D. Warre
Contemporary Literature. Analysis of Jonathan Bazzi's novels
No full textopenDopo una breve panoramica della letteratura italiana degli ultimi vent’anni si analizzano i due romanzi di Jonathan Bazzi "Febbre" e "Corpi minori" dai punti di vista formale, stilistico e tematico. Si discute inoltre il rapporto tra social media, autofiction e autore; nel capitolo 4 si riporta l'intervista che Bazzi ci ha gentilmente concesso, in cui questi argomenti vengono ripresi.
Si individuano alcune differenze che i testi mostrano rispetto alla letteratura moderna, e gli aspetti che hanno in comune con quella contemporanea; nel fare questo si accennano quindi alcune caratteristiche della società che li ha prodotti.The paper starts off with a brief overview of the contemporary Italian literature; then the reader is guided through an analysis of Jonathan Bazzi's novels, "Febbre" ("Fever") and "Corpi minori" ("Minor bodies"), both translated in English and published by Scribe. The relationship between author, autofiction and social media will also be discussed; in chapter four the reader will find the interview Bazzi kindly granted us
Administration and Curricula of the Introductory Graduate Music Research Course
No full textThe introductory research course is an integral part of many graduate music programs, yet there have been few studies that discuss its curricula across institutions. A questionnaire was sent to instructors of the course to identify shared pedagogical approaches among North American schools of music. The survey was divided into sections that prompted respondents to identify issues discussed in the course, including the types and titles of resources, research methodologies, and library use topics. With a response rate of over 40 percent, the survey also contains valuable data concerning the professional identifications of instructors, assignments used for grading, common textbooks, perception of the course’s efficacy, and more. Shared features of the course included the importance of electronic resources; the minimal use of Internet-mediated instruction formats; a strong preference for English-language materials; and a focus on resources such as databases, style guides, collected works, monuments of music, and thematic catalogs over and above others such as repertoire guides, discographies, directories, and iconographies.Peer reviewedThis publication first appeared in Notes Volume 71, Number 3, March 2015, pp. 448-478. This material may not be copied or reposted without explicit permission. Copyright 2015, Jonathan Sauceda
Citizen participation in news
No full textThe process of producing news has changed significantly due to the advent of the Web, which has enabled the increasing involvement of citizens in news production. This trend has been given many names, including participatory journalism, produsage, and crowd-sourced journalism, but these terms are ambiguous and have been applied inconsistently, making comparison of news systems difficult. In particular, it is problematic to distinguish the levels of citizen involvement, and therefore the extent to which news production has genuinely been opened up. In this paper we perform an analysis of 32 online news systems, comparing them in terms of how much power they give to citizens at each stage of the news production process. Our analysis reveals a diverse landscape of news systems and shows that they defy simplistic categorisation, but it also provides the means to compare different approaches in a systematic and meaningful way. We combine this with four case studies of individual stories to explore the ways that news stories can move and evolve across this landscape. Our conclusions are that online news systems are complex and interdependent, and that most do not involve citizens to the extent that the terms used to describe them imply
The Effect of Female Leadership on Contracting from Capitol Hill to Main Street
No full textCode and data to replicate results in: "The Effect of Female Leadership on Contracting from Capitol Hill to Main Street" by Jonathan Brogaard, Nataliya Gerasimova, and Maximilian Rohrer, forthcoming in the Journal of Financial Economics. Please see the README file for details on files and code provided.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV
To what extent is Lemuel Gulliver in Gulliver’s Travels by Jonathan Swift a reflection of the writer with regard to political and religious views, and attitudes toward women and the concept of family?
No full textThis extended essay is an examination of the extent to which the protagonist Lemuel Gulliver in Gulliver’s Travels is a reflection of Jonathan Swift. It involves the exploration of this research question in terms of politics, religion, attitude to women and family; with references to this piece of literature and some secondary resources when necessary. The quotations from published literary criticism are either refuted by examples from the novel or supported in the light of evidence from the novel. Other secondary resources include Swift’s two other prose works, The Modest Proposal and A Letter to a Very Young Lady on Her Marriage, which are referred to briefly for clarification of the evidence. The purpose of this study is to analyse in what ways and to what extent the protagonist is an author-surrogate in the abovementioned ways.
This essay is comprised of two sections, namely “politics and religion” and “women and family”, each focusing on a particular aspect of the investigation. In the first section, Swift’s political and religious standpoint is discussed extensively in order to correctly evaluate Gulliver’s paradigm. By making connections between the beliefs of the author and those of Gulliver, the relation between the two is established to support the claim of this essay. In the second section, the female figures in the novel and Gulliver’s perception of them are inspected. The plot is also taken into consideration in this part of the inquiry although the central focus is on the persona.
In the conclusion, it is validated that Gulliver is a reflection of Jonathan Swift with regard to political and religious vision, and attitude towards women and family, by juxtaposing and assembling the main elements of personification of Gulliver and Jonathan Swift’s personal ideas and experiences
The Effect of Female Leadership on Contracting from Capitol Hill to Main Street
No full textCode and data to replicate results in: "The Effect of Female Leadership on Contracting from Capitol Hill to Main Street" by Jonathan Brogaard, Nataliya Gerasimova, and Maximilian Rohrer, forthcoming in the Journal of Financial Economics. Please see the README file for details on files and code provided.THIS DATASET IS ARCHIVED AT DANS/EASY, BUT NOT ACCESSIBLE HERE. TO VIEW A LIST OF FILES AND ACCESS THE FILES IN THIS DATASET CLICK ON THE DOI-LINK ABOV
Exome sequencing reveals a novel partial deletion in the progranulin gene causing primary progressive aphasia.
No full textAbstract not availableJonathan D Rohrer, Jonathan Beck, Vincent Plagnol, Elizabeth Gordon, Tammaryn Lashley, Tamas Revesz, John C Janssen, Nick C Fox, Jason D Warren, Martin N Rossor, Simon Mead, Jonathan M Schot
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