8,135 research outputs found
Edson Detregiachi Filho: entrevista 1702
No full textEntrevista com Edson Detregiachi Filho, autor do livro A evasão escolar na educação tecnológica: o embate entre percepções subjetivas e objetivas, publicado pela Editora Unesp.Universidade Estadual Paulista (UNESP), Pós-Graduação em Educação, Faculdade de Filosofia e Ciências (FFC), Maríli
066 - Edson Costa Filho
No full textColorado State University - Civil and Environmental Engineering.Includes bibliographical references.Better irrigation water management requires accurate estimates of crop water use. Modeling evapotranspiration (ET) using the surface energy balance approach and remote sensing data has been showing promising results, but the complex nature of heat and momentum transfers among land, plants, and atmosphere has imposed a challenge towards obtaining more accurate crop water consumptive use results. The project aims to improve estimates of crop ET by improving the estimation of sensible heat (H), the most critical component of the surface energy balance, through a combined application of remote sensing data and an aerodynamic temperature approach
The biology and ecology of the Antarctic limpet Nacella concinna
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Previous issue date: 2015Instituto Nacional de Ciência e Tecnologia Antártico de Pesquisas Ambientais (INCT-APA)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Physiological studies suggest that Antarctic marine organisms are adversely affected by rising global temperatures and ocean acidification and have poor prospects for survival. However, according to ecological studies, their vulnerability might be less severe than initially thought. Thus, a realistic forecast of species survival and Antarctic biodiversity should be based on studies from a variety of species under consideration of ecological factors. The limpet Nacella concinna is often found in the rocky intertidal and sublittoral zones of the Antarctic Peninsula and adjacent subantarctic islands. This review summarizes most of the available information on the biology of this limpet, one of the most conspicuous invertebrates of the intertidal zone. There is some evidence that adult N. concinna are physiologically flexible and can acclimate to 3 A degrees C. However, the requirements of the larval stage are poorly known, thus precluding realistic predictions of how elevated temperatures will affect N. concinna populations. Data on physiological performance (righting ability, tenacity and radula rasping rate) under different temperatures could provide a useful baseline for further field investigations on the effects of warming. The species could be used as model organism for investigating the biological effects of ongoing global warming on slow-growing Antarctic ectotherms. Nacella concinna might also be a better biomonitor for polycyclic aromatic hydrocarbons than other Antarctic mollusks.[Suda, Cecilia N. K.; Vani, Gannabathula S.; de Oliveira, Mariana Feijo; Rodrigues, Edson, Jr.; Rodrigues, Edson; Lavrado, Helena P.] Univ Fed Rio de Janeiro, Natl Inst Sci & Technol Antarctic Environm Res IN, Headquarters, BR-21941902 Rio De Janeiro, RJ, Brazil[Suda, Cecilia N. K.; Vani, Gannabathula S.; de Oliveira, Mariana Feijo; Rodrigues, Edson] Universidade de Taubaté (Unitau), Basic Biosci Inst, BR-12030180 Taubate, SP, Brazil[de Oliveira, Mariana Feijo; Rodrigues, Edson, Jr.] Univ Fed Parana, Dept Cell Biol, BR-81990970 Curitiba, Parana, Brazil[Lavrado, Helena P.] Univ Fed Rio de Janeiro, Dept Marine Biol, BR-21941902 Rio De Janeiro, RJ, Brazi
Arginine metabolism of the Antarctic Bivalve Laternula elliptica (King & Broderip, 1831): an ecophysiological approach
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Previous issue date: 2009Universidade Federal do Rio de Janeiro (UFRJ)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Ministério do Meio Ambiente (MMA)Secretariat of the Inter-Ministry Commission for Marine Resources (SeCIRM)The potential aerobic ATP-generating pathway and the argininolytic capacity of the Antarctic bivalve Laternula elliptica in its main tissues were measured by the specific activity of the enzymes malate dehydrogenase (MDH), citrate synthase (CS) and arginase. The kidney showed the major potential for aerobic ATP-generating pathway and argininolytic capacity. High levels of CS and MDH activities indicated that renal tissue can be involved in activities that require a lot of energy such as excretion of metabolic end products, amino acids catabolism or even gluconeogenic activities related to inter-tissue metabolism. The fact that kidneys are the main site for arginase activity is very unusual for mollusks and could be related to the living habits of L. elliptica. Genetic expression of the L. elliptica renal arginase could be controlling the levels of l-arginine and forming urea in the excretory organ, which may not have its physiological functions directly affected by the seasonal retraction of its siphons. Compared to the bivalve Dreissena polymorpha, renal arginase of L. elliptica is more resistant to inhibition by copper and cadmium. This could be related to naturally high levels of these metals in the Antarctic marine environment and its bioaccumulation in the renal tissue of L. elliptica, as a probable advantage to its environmental adaptation. Different from other Antarctic animals that feed on Krill, the arginase of L. elliptica is much more sensitive to fluoride inhibition. However, diet composition of L. elliptica would be expected to be variable site to site and its high sensitivity to fluoride inhibition may be a matter of concern in areas near ornithogenic soils subjected to ice-melting processes.[Rodrigues, Edson; da Silva Santos, Marcela Rosana; Rodrigues Junior, Edson; Gannabathula, Vani] Universidade de Taubaté (Unitau), Biochem Lab, Basic Inst Biosci, BR-12030180 Taubate, SP, Brazil[Lavrado, Helena Passeri] Univ Fed Rio de Janeiro, UFRJ, CCS, BR-21949900 Rio De Janeiro, Brazi
Interactions of temperature, salinity and diesel oil on antioxidant defense enzymes of the limpet Nacella concinna
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Previous issue date: 2015Ministério do Meio Ambiente (MMA)Ministério da Ciência, Tecnologia e Inovação (MCTI)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Secretariat of the Inter-ministerial Commission for the Resources of the Sea (SeCIRM)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Instituto Nacional de Ciência e Tecnologia Antártico de Pesquisas Ambientais (INCT-APA)The intertidal and subtidal environments in the Antarctic Peninsula are vulnerable to pollutants, such as diesel oil, a commonly used fuel. Nacelle concinna is capable of accumulating polycyclic aromatic hydrocarbons and is a potential biomonitor of diesel oil contamination. This work investigates the interaction of diesel oil, temperature and salinity on the activity of antioxidants enzymes defense of the gills, foot muscle and digestive glands. Upregulation of superoxide dismutase occurred in the three tissues by warming. The foot muscle catalase and the gill glutathione reductase were upregulated only by diesel. The inability to upregulate catalase and glutathione S-transferase in the digestive gland, as well as the increase of lipoperoxidation, suggested that this gland is more susceptible to the deleterious effects from oxidative stress. (C) 2015 Elsevier Ltd. All rights reserved.[de Oliveira, Mariana Feijo; Rodrigues Junior, Edson; Donatti, Lucelia] Univ Fed Parana, Dept Cell Biol, BR-81530130 Curitiba, Parana, Brazil[de Oliveira, Mariana Feijo; Rodrigues Junior, Edson; Suda, Cecilia N. K.; Vani, Gannabathula S.; Rodrigues, Edson] Universidade de Taubaté (Unitau), Basic Biosci Inst, BR-12030180 Taubate, SP, Brazil[Lavrado, Helena P.] Univ Fed Rio de Janeiro, Dept Marine Biol, BR-21941902 Rio De Janeiro, RJ, Brazi
Temas atuais de direito das famílias
No full text- Divulgação dos SUMÁRIOS das obras recentemente incorporadas ao acervo da Biblioteca Ministro Oscar Saraiva do STJ. Em respeito à Lei de Direitos Autorais, não disponibilizamos a obra na íntegra.- Localização na estante: 347.6(81) T278d- Walsir Edson Rodrigues Júnior é coordenador da obra
Metabolic responses of the Antarctic fishes Notothenia rossii and Notothenia coriiceps to sewage pollution
No full textMade available in DSpace on 2019-09-12T16:53:28Z (GMT). No. of bitstreams: 0
Previous issue date: 2015Ministério do Meio Ambiente (MMA)Ministério da Ciência, Tecnologia e Inovação (MCTI)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Secretariat of the Inter-ministerial Commission for the Resources of the Sea (SeCIRM)Instituto Nacional de Ciência e Tecnologia Antártico de Pesquisas Ambientais (INCT-APA)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)The present study aimed to assess the sewage effects of the Brazilian Antarctic Station Comandante Ferraz, Admiralty Bay, King George Island, on the hepatic metabolism (energetic, antioxidant, and arginase levels) and levels of plasma constituents of two Antarctic fish species Notothenia rossii and N. coriiceps. The bioassays were conducted under controlled temperature (0 degrees C) and salinity (35 psu), exposing the fish for 96 h, to sewage effluent diluted in seawater to 0.5 % (v/v). Liver homogenates were tested for the specific activities of the enzymes glucose-6-phosphatase (G6Pase), glycogen phosphorylase (GPase), hexokinase, citrate synthase, lactate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, superoxide dismutase, glutathione reductase, catalase, and arginase. Plasma levels of glucose, triacylglycerides, cholesterol, total protein, albumin, chloride, magnesium, calcium, and inorganic phosphate were also determined. In N. rossii, the decrease in citrate synthase and the increase in G6Pase and GPase suggested that the sewage effluent activated glycogenolysis and hepatic gluconeogenesis, whereas is N. coriiceps, only G6Pase levels were increased. In N. rossii, sewage effluent induced hypertriglyceridemia without modulating glucose plasma levels, in contrast to N. coriiceps, which developed hypoglycemia without elevating plasma triglyceride levels. The decrease in glutathione reductase levels in N. coriiceps and in superoxide dismutase and catalase in N. rossii suggest that these two species are susceptible to oxidative stress stemming from the production of reactive oxygen species. An increase in magnesium in N. rossii and a decrease in N. coriiceps showed that sewage effluent compromised the control of plasma levels of this cation. Although phylogenetically close, both species of Antarctic fish exhibited different metabolic responses to the sewage effluent, with N. coriiceps showing greater susceptibility to the toxic effects of the pollutants. The present study suggests that the biochemical responses of these two species are potential indicators of metabolic changes caused by sewage effluents.[Rodrigues, Edson, Jr.; Feijo-Oliveira, Mariana; Kawagoe Suda, Cecilia Nohome; Vani, Gannabathula Sree; Rodrigues, Edson] Universidade de Taubaté (Unitau), Inst Basic Biosci, BR-12030180 Taubate, SP, Brazil[Donatti, Lucelia] Univ Fed Parana, Dept Cell Biol, BR-81530130 Curitiba, PR, Brazil[Lavrado, Helena Passeri] Univ Fed Rio de Janeiro, Dept Marine Biol, BR-21941902 Rio De Janeiro, RJ, Brazi
Análise de metabólitos secundários em culturas de Pestalotiopsis sp e Aspergillus aculeatus.
No full textSesquiterpenos da classe dos cariofilenos são compostos
químicos já isolados de fungos do gênero Pestalotiopsis no nosso grupo de
estudos. No presente trabalho foram desenvolvidas metodologias de cultivo
e análise de espécies de Pestalotiopsis, isoladas das plantas Pinus taeda,
Swietenia macrophyla, e Khaya invorenses. Diferentes procedimentos de
extração e análise desses extratos, empregando LC/MS e GC/MS, foram
otimizados. Esses procedimentos visaram a detecção desses sesquiterpenos
sem a necessidade de isolamento. Posteriormente foi delineada uma curva
de produção desses compostos variando-se o tempo de cultivo, para
posterior quantificação. A produção de compostos voláteis produzidos
pelas diferentes espécies de Pestalotiopsis, foi avaliada usando a técnica de
extração por hidrodestilação (através do extrator de Clevenger). Um
sesquiterpeno cariofileno foi o principal composto identificado.
Um outro estudo dos metabólitos secundários produzidos pelo
fungo Aspergillus aculeatus, isolado da planta hospedeira Melia azedarach,
foi feito tendo como alvo pré-determinado os policetídeos aromáticos
diméricos como as Aurasperonas A e B, Fonsesinona A e B, Orlandina e
Kotanina. Uma curva de produção desses policetídeos mostrou que os
monômeros não são detectados nos tempos iniciais de cultivo, indicando
uma rápida dimerização no meio ou a sua biossíntese já na forma
dimerizada. Experimentos de biotransformação mostrou que o fungo possui
capacidade de modificar certos substratos, quando cultivado em meios
artificiais.Cariophylene sesquiterpenes are chemical compounds isolated
from fungi of the Pestalotiopis genus in early studies developed in our
research group. In the present work, a cultivation and analysis methodology
were developed for these compounds in Pestalotiopsis, isolated from plants
Pinus taeda, Swietenia macrophyla and Khaya invorenses. Different
procedures were optimized for the extraction and analysis of the extracts
employing liquid and gas chromatography coupled with mass spectrometry
techniques (LC/MS and GC/MS). These studies had the intention of to
detect the sesquiterpenes in fungal extracts without theys of isolation, and
to check their production profile during a fixed cultivation period. The
production of volatile organic compounds by the different species of
Pestalotiospsis was also evaluated using hydrodestillation in Clevenger
apparatus. The sesquiterpene cariophyllene was the main compound
identified.
Another study involving the secondary metabolites produced
by Aspergillus aculeatus, a fungus isolated from the host plant Melia
azedarach, was carried out. The target compounds in these studies were the
dimeric aromatic poliketides Aurasperones A and B, Fonsesinones A and
B, Orlandine and Kotanin. A poliketide production curve, obtained using
HPLC, showed that the monomers are not detected during the early stage of
development, indicating that probably the dimers are rapidly formed by the
fungus or they are biosynthesized in the form already dimeric. Experiments
of biotransformation showed that fungi possesss capacity to modify certain
substracts, when cultivated in artificial ways
Evidence of metabolic microevolution of the limpet Nacella concinna to naturally high heavy metal levels in Antarctica
No full textMade available in DSpace on 2019-09-12T16:53:35Z (GMT). No. of bitstreams: 0
Previous issue date: 2017Ministério do Meio Ambiente (MMA)Ministério da Ciência, Tecnologia e Inovação (MCTI)Secretaria da Comissão Interministerial para os Recursos do Mar (SECIRM)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)The gastropod Nacella concinna is the most conspicuous macroinvertebrate of the intertidal zone of the Antarctic Peninsula and adjacent islands. Naturally high levels of copper and cadmium in coastal marine ecosystems are accumulated in N. concinna tissues. We aimed to study the effects of metal cations on N. concinna arginase in the context of possible adaptive microevolution. Gills and muscle had the highest argininolytic activity, which was concentrated in the cytosol in both tissues. Gills had the highest levels of arginase and may be involved in the systemic control of L-arginine levels. The relatively high argininolytic activity of the N. concinna muscular foot, with K-M = 25.3 +/- 3.4 mmol L-1, may be involved in the control of L-arginine levels during phosphagen breakdown. N. concinna arginases showed the following preferences for metal cations: Ni2+ > Mn2+ > Co2+ > Cu2+ in muscle and Mn2+ > Cu2+ in gills. Cu2+ activation is a unique characteristic of N. concinna arginases, as copper is a potent arginase inhibitor. Cu2+ partly neutralized N. concinna arginase inhibition by Cd2+, worked synergistically in muscle arginase activation by Co2+ and neutralized muscle arginase activation by Ni2+. Mn2+ was able to activate muscle arginase in the presence of Fe3+ and Pb2+. The selection of arginases that are activated by Cu2+ and resistant to inhibition by Cd2+ in the presence of Cu2+ over evolutionary timescales may have favored N. concinna occupation of copper- and cadmium-rich niches. (C) 2016 Elsevier Inc. All rights reserved.[de Oliveira, Mariana Feijo; Rodrigues Junior, Edson; Kawagoe Suda, Cecilia Nahomi; Vani, Gannabathula Sree; Rodrigues, Edson] Universidade de Taubaté (Unitau), Basic Biosci Inst[Donatti, Lucelia] Univ Fed Parana, Dept Cell Biol, Curitiba, Parana, Brazil[Lavrado, Helena Passeri] Univ Fed Rio de Janeiro, Dept Marine Biol, Rio De Janeiro, Brazi
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