12 research outputs found
Uji Aktivitas Antikanker Ekstrak Mentimun Laut (Holothuroidea) dan Bintang Ular Laut (Ophiuroidea) Terhadap Sel Kanker Serviks HeLa
No full textKanker merupakan penyakit yang memiliki prevalensi tinggi di dunia dan dapat berujung pada kematian. Salah satu jenis kanker yang sering menyerang wanita adalah kanker serviks. Mentimun laut dan bintang ular laut diketahui mengandung senyawa yang berpotensi dalam farmakologis, salah satunya sebagai antikanker. Penelitian ini bertujuan untuk mengetahui aktivitas antikanker dari ekstrak air mentimun laut dan bintang ular lautterhadap sel kanker serviks HeLa. Metode yang dilakukan dalam penelitian ini adalah pemberian ekstrak mentimun laut dan bintang ular laut pada sel kanker serviks HeLa. Parameter yang diuji meliputi viabilitas sel menggunakan reagen WST-1, kemampuan menginduksi apoptosis sel menggunakan pewarna AnnexinV/PI, dan penghambatan siklus sel menggunakan pewarna PI dengan analisa flow cytometry. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 replikasi. Perlakuan yang dilakukan yakni kontrol (0 μg/mL), dosis I (500 μg/mL), dosis II (250 μg/mL), dan dosis III (100 μg/mL). Analisis data hasil analisa flow cytometry menggunakan software CellQuestPro. Data viabilitas sel dianalisis dengan One way Anova dengan uji lanjutan tukey (α = 0.05) pada minitab 16. Hasil dari uji viabilitas sel menunjukan bahwa kedua ekstrak mampu menghambat pertumbuhan sel HeLa. Mentimun laut dapat menghambat siklus sel HeLa pada fase G2/M, sedangkan bintang ular laut pada fase G0/G1. Kedua esktrak mampu menginduksi apoptosis pada sel HeLa. Kesimpulan yang diperoleh adalah mentimun laut dan bintang ular laut berpotensi sebagai antikanker pada sel HeLa
Antibody production of wild-type and enzyme V279F variants of PAF-AH as a risk factor for Cardiovascular disease
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The role of Moringa oliefera-Albumin combination as a traditional medicine to control the development of SDF-1 and TER-119+VLA-4+ in diabetes mice models
No full textProtective layer β-cyclodextrin within peanut (Arachis hypogaea L.) shells’ nanoparticles enhances intracellular stable fluorescence for bioimaging applications: An in vitro and in silico study
No full textOwing to their excellent fluorescence behavior in the past decades, carbon nanoparticles have gained remarkable attention for a wide range of biomedical applications. However, the synthesis and solubility of carbon nanoparticles in an aqueous solution remains a challenging area of study. In this work, we synthesized carbon nanoparticles from peanut (Arachis hypogaea L.) shells with a protected layer of β-cyclodextrin (β-CD) as a highly stable fluorescent material for bioimaging applications. These nanoparticles possess highly stable photoluminescence and optical absorption properties in an aqueous solution. Additionally, in vitro kinetic stability confirmed that β-CD-coated carbon nanoparticles are stable under serum-rich environments and exhibit antihemolytic properties. Predominantly, in vitro cytotoxicity studies clearly demonstrated that β-CD-coated carbon nanoparticles possess cytotoxicity, rapid intracellular uptake, and stable fluorescence under HeLa cells. Moreover, an in-silico study confirmed that β-CD-coated carbon nanoparticles could specifically interact with the target protein of CDK-2, which plays a role in inhibiting cancer cell growth. Hence, this new study has shown that in tracking cancer cells, high-value-added peanut (Arachis hypogaea L.) shells with a β-CD protective layer are effective in improving solubility and intracellular fluorescence
Think-Aloud Protocol as a Method for Exploring and Understanding the Argumentation Skills of Pre-Service Science Teachers
No full textArgumentation is a core component of critical thinking, one of the essential 21st-century skills. However, exploring this skill remains a challenge, often due to limitations in the available assessment methods. This study aims to offer a solution by utilizing the think-aloud protocol to explore and better understand the argumentation skills of Pre-Service Science Teachers (PSTs). A total of 30 PSTs from a state university in Indonesia participated in the research. Data collection and analysis through the think-aloud protocol were conducted in several stages: procedural briefing, implementation, coding and video analysis, and data interpretation. The findings revealed that the think-aloud protocol was effective in providing deeper insights into argumentation skills and yielded more detailed and accurate data across five key components: claims, evidence, reasoning, counterarguments, and rebuttals. On average, the PSTs demonstrated beginner-level argumentation skills, with the strongest performance in the claim component and weaker results in supporting aspects. Specifically, 5 participants were categorized as proficient, 9 as advanced, 6 as intermediate, and 10 as beginners. These results highlight the need for more effective learning strategies that provide PSTs with greater opportunities to practice constructing and critiquing arguments
Swietenia mahagoni Leaves Ethanolic Extract: In vitro anti-Oxidant Activity, Active Compound Identification and in silico Prediction as AKT-1 and MDM2 Protein Inhibitor
No full textيدعم الارتباط الوثيق بين الممارسات التقليدية والخصائص الدوائية لهذه النباتات استمرار استخدامها في علاج مختلف الحالات الصحية. قيّمت هذه الدراسة وتوقعت المركب النشط في المستخلص الإيثانولي لأوراق سويتينيا ماهاغوني وفعاليته في تثبيط نمو الخلايا السرطانية. شمل التحليل قياس تثبيط الجذور الحرة DPPH، ومحتوى الفينول والفلافونويد الكلي، وتقييم تشابه الأدوية، ودراسات الالتحام الجزيئي. تشير النتائج إلى أن المستخلص الإيثانولي لأوراق S. mahagoni يُظهر خصائص مضادة للأكسدة نظرًا لاحتوائه على مركبات فينولية وفلافونويدية مثل الكيرسيترين، و(+)-ar-Turmerone، وهايبروسيد، والتي تُلبي أيضًا معايير ليبينسكي. بالإضافة إلى ذلك، قد تعمل هذه المركبات كمثبطات لـ MDM2 أو AKT-1، مما قد يُعيق MDM2 وAKT-1 ويُحفز موت الخلايا المبرمج في الخلايا السرطانية. ينبغي إجراء المزيد من الأبحاث في المختبر للتحقق من فعالية المركبات المدروسة.The strong correlation between traditional practices and the pharmacological properties of these plants supports their continued use in treating various health conditions. This study evaluated and predicted the active compound in the ethanolic extract of Swietenia mahagoni leaves and their potency for inhibiting cancer cell growth. The analysis included measuring DPPH free radical inhibition, total phenolic and flavonoid content, drug-likeness evaluation, and molecular docking studies. Findings suggest that the ethanolic extract of S. mahagoni leaves ethanolic extract exhibits antioxidant properties due to its content of phenolic and flavonoid compounds such as Quercitrin, (+)-ar-Turmerone, and Hyperoside, which also meet Lipinski's criteria. Additionally, these compounds might act as inhibitors of MDM2 or AKT-1, potentially blocking MDM2 and AKT-1 and inducing apoptosis in cancer cells. Further research should be conducted in vitro to validate the activity of the studied compounds
Pelatihan Guru IPA SMP dalam Membuat Lembar Kerja Peserta Didik Berbasis Culturally Responsive Teaching: Upaya Mewujudkan Implementasi Kurikulum Merdeka sebagai Kurikulum Nasional
No full textKegiatan ini bertujuan untuk meningkatkan pengetahuan dan keterampilan guru IPA SMP dalam menyusun Lembar Kerja Peserta Didik (LKPD) berbasis Culturally Responsive Teaching (CRT) untuk mendukung implementasi Kurikulum Merdeka. Pelaksanaan melibatkan 45 guru anggota MGMP IPA Kabupaten Pasuruan menggunakan desain one-group pretest-posttest. Metode pelatihan terdiri dari blended learning yang mencakup workshop luring dan pendampingan daring melalui Google Classroom. Kegiatan melibatkan pengenalan CRT, praktik penyusunan LKPD, hingga pengunggahan karya di Platform Merdeka Mengajar. Hasil menunjukkan peningkatan signifikan pada pengetahuan guru, dengan skor rata-rata posttest berada di kategori sangat baik (55,56%). Keterampilan guru dalam menyusun LKPD berbasis CRT juga meningkat, terutama dalam aspek integrasi budaya lokal dan strategi pembelajaran. Pembahasan mengungkapkan perlunya pendampingan berkelanjutan untuk penguasaan prinsip CRT. Rekomendasi mencakup penguatan pelatihan pada aspek integrasi budaya dan kolaborasi guru-peserta didik untuk efektivitas pembelajaran berbasis budaya.
Training of Junior High School Science Teachers in Creating Student Worksheets Based on Culturally Responsive Teaching: Efforts to Realize the Implementation of the Independent Curriculum as a National Curriculum
Kegiatan ini bertujuan untuk meningkatkan pengetahuan dan keterampilan guru IPA SMP dalam This program aimed to enhance junior high school science teachers' knowledge and skills in developing Student Worksheets (LKPD) based on Culturally Responsive Teaching (CRT) to support the implementation of the Independent Curriculum. The program engaged 45 teachers from the MGMP IPA in Pasuruan Regency, using a one-group pretest-posttest design. The training method combined blended learning, including face-to-face workshops and online mentoring via Google Classroom. Activities included CRT introduction, LKPD design practice, and uploading teacher-created worksheets to the Merdeka Mengajar Platform. The results showed a significant improvement in teachers’ knowledge, with an average posttest score categorized as excellent (55.56%). Teachers’ skills in developing CRT-based LKPD also improved, especially in integrating local cultural values and implementing teaching strategies. The discussion highlighted the need for continuous mentoring to deepen understanding of CRT principles. Recommendations include strengthening training in cultural integration aspects and fostering teacher-student collaboration for more effective culturally-based learning.
Preparation of Virgin Coconut Oil (VCO) Using the Pineapple Enzymatic Method
No full textVirgin Coconut Oil (VCO) merupakan minyak nabati yang diekstrak dari endosperma kelapa segar dan matang tanpa penyulingan kimia atau panas tinggi. VCO dapat diproduksi dengan beberapa metode, salah satunya adalah metode enzimatik nanas. Hasil produksi VCO enzimatik nanas memiliki keunggulan dibandingkan metode lainnya. Nanas merupakan tanaman yang relatif mudah dibudidayakan di Indonesia, namun pemanfaatan nanas masih belum maksimal, biasanya hanya sebagai olahan makanan atau minuman yang dapat dikonsumsi langsung. Penelitian ini bertujuan untuk mengetahui cara produksi VCO menggunakan metode enzimatik dari buah nanas dan parameter fisik VCO yang dihasilkan. Metode yang digunakan dalam penelitian ini adalah metode campuran. Hasil penelitian ini menghasilkan minyak sebanyak 40 ml dengan kejernihan bening, aroma sedikit menyengat khas kelapa, dan memiliki rasa khas minyak kelapa
The V279F polymorphism might change protein character and immunogenicity in Lp-PLA2 protein
No full textBackground: Lipoprotein-associated phospholipase A2 (Lp-PLA2) plays a strong role in the occurrence of certain cardiovascular disease processes. Polymorphisms at the protein level are also estimated to correlate with increased risk factors for heart attacks. One such polymorphism is the V279F polymorphism in Lp-PLA2 which results in a change in enzyme performance capability. This in turn implies a reduced risk of acute myocardial infarct (AMI) in Korean and Indonesian subpopulations. Aim: This study aimed to analyze changes in protein properties, structure, energy stability, epitope, and immunogenicity that are due to the substitution of the Valine (V) amino acid at position 279 to Phenylalanine (F) in the Lp-PLA2 protein. Methodology: The role of Lp-PLA2 in the cardiovascular process and in AMI was analyzed based on the protein-protein network according to the BioGRID, MENTHA, and STRING databases. Protein properties and energy stability were examined by FoldX; this was followed by identification of epitope using ElliPro. The immunogenicity was evaluated in vivo by injecting the protein into mice and subsequently measuring their antibody production using the ELISA technique. Results: The substitution of Valine for Phenylalanine was predicted to increase protein stability and epitope shifts. Further studies on animal experiments exhibit that the 279V variant is able to induce IgG production better than the 279F variant. Conclusion: Based on these data, it can be concluded that the V279F polymorphism influences the surface structure, energy stability, epitope and immunogenicity of the Lp-PLA2 protein. The changes in the immunogenicity and epitope shift indicated that the protein is valuable as a biomarker for use in acute myocardial infarct. The results of this study provide an opportunity to develop monoclonal antibodies that are specifically able to identify V279F polymorphisms as a predictor of the risk of acute myocardial infarct. Keywords: Epitope shifting, Cardiovascular, PAF-AH, Phospholipase A2, PLA2G
