205 research outputs found

    Cloning and expression of three ladA-type alkane monooxygenase genes from an extremely thermophilic alkane-degrading bacterium Geobacillus thermoleovorans B23

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    An extremely thermophilic bacterium, Geobacillus thermoleovorans B23, is capable of degrading a broad range of alkanes (with carbon chain lengths ranging between C11 and C32) at 70 A degrees C. Whole-genome sequence analysis revealed that unlike most alkane-degrading bacteria, strain B23 does not possess an alkB-type alkane monooxygenase gene. Instead, it possesses a cluster of three ladA-type genes, ladA alpha(B23), ladA beta(B23), and ladB (B23), on its chromosome, whose protein products share significant amino acid sequence identities, 49.8, 34.4, and 22.7 %, respectively, with that of ladA alkane monooxygenase gene found on a plasmid of Geobacillus thermodetrificans NG 80-2. Each of the three genes, ladA alpha(B23), ladA beta(B23), and ladB (B23), was heterologously expressed individually in an alkB1 deletion mutant strain, Pseudomonas fluorescens KOB2 Delta 1. It was found that all three genes were functional in P. fluorescens KOB2 Delta 1, and partially restored alkane degradation activity. In this study, we suggest that G. thermoleovorans B23 utilizes multiple LadA-type alkane monooxygenases for the degradation of a broad range of alkanes

    Reflecting the theatralization of life in the prospects of transhumanism

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    The crisis state of society at the beginning of the 21st century is increasingly described as chaos. At the same time, the desire of people to theatricalization of many types of their activities remains unchanged. This gives grounds to consider the theatricality of being as a sociocultural constant, immanently inherent in humanity. The fact that it persists even during periods of cardinal changes, social transformations, civilizational shifts, breaking cultural stereotypes, destruction of traditional institutions and discreditation of established ideals can be explained by the fact that theatricalization of life allows people to cope with sociopsychological discomfort and existential fears, caused by the feeling of total chaos. At the same time, people are faced with the problem of increasingly complex self-identification, which is largely facilitated by the ideas of transhumanism, which actively shatter the already weak notions of what a human being is. One of the approaches to solving this scientific problem is proposed to consider human activity in the context of theatricality of being. The purpose of the article is to comprehend the practice of theatricalization of various types of human activity in the context of the ideas of transhumanism. The methodological strategy of this research is based on the concept of theatricality of socio-communicative manifestations of culture. Analysis of the theoretical material showed that in the conditions of transhumanism, the development of criteria that make it possible to determine whether a human is still a human or has already turned into something else becomes an urgent task. One of these markers is proposed to consider the desire of a human to theatricalization of various types of their activities. Being one of the sociocultural constants immanently inherent in humanity, theatricality will be present in people's lives as long as they remain human, as long as they feel the need to articulate their social communication

    Социально-философские основания театрализации политической сферы

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    Мета дослідження – виявлення і осмислення соціально-філософських засад театралізації політичної сфери. Виходячи з гіпотези про те, що театралізація сприяє підвищенню ефективності комунікацій в сфері політики, в якості методологічної стратегії дослідження обрано концепцію театральності соціокомунікативних проявів культури (на базі методів соціокультурного аналізу). Цей підхід дозволив з’ясувати, що поєднання в політичних «виставах» елементів гри, перформансу, ритуалу, карнавалу, маскараду сприяє: приверненню уваги опонентів; демонстрації солідарності із протестним рухом; формуванню іміджу відповідно до ролі / амплуа, яку політик виконує у політичному «театрі»; створенню додаткових каналів комунікації політика із «публікою» (народом, електоратом). В якості одного з таких каналів розглядаються костюмні прикраси і аксесуари, які є не лише доповненням до іміджу політика, а й важливим елементом драматургії. Дозволяючи заміняти пряме повідомлення на знаково-символьну форму, прикраси сприяють наданню процесу комунікації ігрового, театрального формату. Це, з одного боку, пожвавлює процес спілкування політика із «публікою», сприяє розширенню аудиторії, але, з іншого боку – привносить в процес передачі інформації елемент інтерпретації. Цією обставиною активно користуються мас-медіа, посилюючи свою роль у політичному «театрі». З’ясовано також, що певною мірою театральність завжди була притаманна політиці. Проте театральне не завжди в ній превалює: прагнення до театралізації зростає по мірі розділення політичної сфери на публічну і «приховану». Посилення театральних проявів у сучасній політичній сфері вказує на те, що саме така тенденція набуває розвитку в соціальних відносинах. Це в свою чергу обумовлює появу нових технологій і форматів комунікації в системі «влада – суспільство».Public policy is always focused on finding the most effective ways to communicate with the broad masses (society, population, electorate). Historical experience shows that one of such methods is the introduction of theatrical elements into the communication process. The purpose of this study is to identify and comprehend the social and philosophical foundations of the theatricalization of the political sphere. The concept of theatricality of sociocommunicative manifestations of culture was chosen as a methodological research strategy (based on methods of sociocultural analysis). This approach made is possible to find out that the combination of the elements of the game, performance, ritual, carnival, masquerade in political “spectacles” helps to: attract the attention of opponents; demonstrations of solidarity with the protest movement; the formation of the image of the politician in accordance with the role that he plays in the political “theater”; creating additional channels of communication policy with the “public”. One of these channels is costume jewelry and accessories, which are not only an addition to the image of a politician, but also an important dramatic element. By allowing the replacement of a direct message with a sign-symbolic form, jewelry helps to impart a gaming, theatrical format to the communication process. On the one hand, it helps to expand the audience, but, on the other hand, it introduces an element of interpretation into the process of information transfer. This circumstance is used by the mass media, strengthening its role in the political “theater”. It has also been established that to a certain extent theatricality has always been inherent in politics. But the theatrical component does not always prevail in it: the desire for theatricality increases with the division of the political sphere into public and “hidden”. Strengthening theatrical manifestations in the modern political sphere indicates that this is the trend that is developing in social relations. This, in turn, determines the emergence of new technologies and communication formats in the system “power – society”.Цель исследования – выявление и осмысление социально-философских оснований театрализации политической сферы. Исходя из гипотезы о том, что театрализация способствует повышению эффективности коммуникаций в сфере политики, в качестве методологической стратегии исследования выбрана концепция театральности социокоммуникативных проявлений культуры (на базе методов социокультурного анализа). Этот подход позволил выяснить, что сочетание в политических «спектаклях» элементов игры, перформанса, ритуала, карнавала, маскарада способствует: привлечению внимания оппонентов; демонстрации солидарности с протестным движением; формированию имиджа политика в соответствии с ролью / амплуа, которую он исполняет в политическом «театре»; созданию дополнительных каналов коммуникации политика с «публикой» (народом, электоратом). В качестве одного из таких каналов рассматриваются костюмные украшения и аксессуары, которые являются не только дополнением к имиджу политика, но и важным элементом драматургии. Позволяя заменять прямое сообщение на знаково-символьную форму, украшения способствуют приданию процессу коммуникации игрового, театрального формата. Это, с одной стороны, способствует расширению аудитории, но, с другой стороны – привносит в процесс передачи информации элемент интерпретации. Этим обстоятельством пользуются масс-медиа, усиливая свою роль в политическом «театре». Установлено также, что в определённой степени театральность всегда была присуща политике. Но театральное не всегда в ней превалирует: стремление к театрализации возрастает по мере разделения политической сферы на публичную и «скрытую». Усиление театральных проявлений в современной политической сфере указывает та то, что именно такая тенденция получает развитие в социальных отношениях. Это, в свою очередь, обусловливает появление новых технологий и форматов коммуникации в системе «власть – общество»

    Social Work with Victims of Violence in Home Environment

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    KUČEROVÁ, Lada. Sociální práce s oběťmi násilí v domácím prostředí. Zlín: Univerzita Tomáše Bati ve Zlíně: Fakulta humanitních studií, obor Sociální pedagogika Brno, 2014. Bakalářská práce. Ve své bakalářské práci se autorka zabývá tématem domácího násilí z pohledu sociální práce. V teoretické části vymezuje základní charakteristiku pojmů souvisejících s problematikou domácího násilí, objasňuje, kdo je pachatelem a kdo obětí domácího násilí, vymezuje pojem krizová intervence její etapy, formy i právní aspekty. Součástí práce je legislativa zabývající se problematikou domácího násilí v České republice, organizace, které pomáhají řešit domácí násilí, se zaměřením na město Znojmo. V praktické části autorka využije případových studií, kazuistik, kdy oběťmi i svědky domácího násilí byli dospělí, děti i senioři.KUČEROVÁ, Lada. Social Work with Victims of Violence in Home Environment. Zlín: Tomas Bata University in Zlín, Faculty of Humanities, Social Pedagogy Brno, 2014. Bachelor's thesis. The author deals with the issue of domestic violence from the point of view of social work in the thesis. In the theoretical part the author defines basic characteristics of terms related to the issue of domestic violence, clarifies who is the perpetrator and who is the victim of domestic violence and defines the term crisis intervention its stages, forms and legal aspects. It includes legislation dealing with the issue of domestic violence in the Czech Republic and organizations which help deal with domestic violence focusing on the town of Znojmo. In the practical part the author uses case studies and case reports, in which the victims and witnesses of domestic violence were adults, children and seniors. In the conclusion the author provides an analysis, evaluation and comparison of individual case reports, in order to compare and find possible similarities and differences between individual cases.Katedra pedagogiky a psychologi

    Interactive simulations in the acquisition of 9th grade physics topic “How do we use electricity in everyday life?

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    Pieredzes darbs Interaktīvās simulācijas 9. klases fizikas temata "Kā mēs ikdienā izmantojam elektrību?" apguvē. Darba autore: Lada Bumbure, darba vadītāja: Dr. phys. Inese Dudareva. Darbs ir uzrakstīts latviešu valodā uz 52 lappusēm, ietver 30 attēlus, 3 tabulas, 31 literatūras avotu un 1 pielikumu. Darba mērķis ir apkopot un izvērtēt pieredzi par interaktīvo simulāciju praktisko pielietošanu 9. klases fizikas temata “Kā mēs ikdienā izmantojam elektrību?” apguvē. Darbā tiek veikta zinātniskās un metodiskās literatūras apkopošana par interaktīvo simulāciju lietošanu mācību procesā, apkopota un izvērtēta autores praktiskā pieredze temata plānošanā, mācību procesa organizēšanā un skolēnu snieguma vērtēšanā, kā arī apkopoti metodiski ieteikumi temata "Kā mēs ikdienā izmantojam elektrību?" apguvē, izmantojot interaktīvās simulācijas.Teacher’s experience work Interactive simulations in the acquisition of 9th grade physics topic “How do we use electricity in everyday life?". Author of the work: Lada Bumbure, supervisor: Dr. phys. Inese Dudareva. The work is written in Latvian on 52 pages, includes 30 pictures, 3 tables, 31 literary sources and 1 appendix. The aim of the work is to collect and evaluate the experience of the practical application of interactive simulations in the acquisition of 9th grade physics topic "How do we use electricity in everyday life?". The work collects scientific and methodical literature on the use of interactive simulations in the learning process, collects and evaluates the author's practical experience in planning the topic, organizing the learning process, and evaluating the performance of students, as well as collects methodological recommendations on the topic "How do we use electricity every day?" acquisition through interactive simulations

    Dramatic works of Lada Kastelan

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    U diplomskome je radu prikazano dramsko stvaralaštvo Lade Kaštelan. Budući da je riječ o autorici koja je u hrvatsku dramsku književnost ušla s generacijom koju povjesničari književnosti najčešće nazivaju sintagmom suvremena hrvatska drama ili mlada hrvatska drama, pojašnjen je termin postmodernizam te su iznesena važna obilježja suvremene hrvatske drame. S obzirom na to da se Lada Kaštelan ističe kao jedna od začetnica ženskoga pisma u suvremenoj hrvatskoj drami i kazalištu, autorica je smještena u kontekst hrvatskoga dramskog ženskog stvaralaštva. Predstavljena je njezina prva knjiga dramskih tekstova – Pred vratima Hada, dok je najveći dio rada posvećen dramama A tek se vjenčali, Adagio, Posljednja karika i Giga i njezini, sabranima u autoričinoj drugoj zbirci – Četiri drame. Prikazan je jedinstveni dramski svijet koji autorica ostvaruje u spomenutoj zbirci te su sve četiri drame sagledane u kontekstu suvremenoga hrvatskog ženskog pisma. Središnji dio rada posvećen je postmodernističkim elementima u ciklusu Četiri drame. Riječ je o ženskim likovima u dramama koji su prikazani kroz obiteljske i bračne odnose, zatim o postmodernističkome oblikovanju dramskoga vremena i prostora koje je ostvareno prožimanjem realne i irealne razine, te o intertekstualnosti u dramama budući da se Lada Kaštelan u Posljednjoj karici i u Gigi i njezinima poslužila predlošcima iz hrvatske književnosti. Spomenuti su elementi analizirani u svakoj od četiriju drama i iscrpno obrađeni u zasebnim poglavljima. Analiza pokazuje da su upravo oni, iako neponovljivi i u svakoj drami oblikovani na jedinstveni način, presudni za poznavanje autoričine poetike i za razumijevanje njezinih drama. Rad potvrđuje važnost Lade Kaštelan za suvremeno dramsko stvaralaštvo.This master’s thesis deals with dramatic works of Lada Kastelan. Since she is an author who appeared in Croatian dramatic literature together with a generation which literary historians usually call modern Croatian drama or young Croatian drama, this thesis will explain the term postmodernism and it will state the important features of modern Croatian drama. Lada Kastelan stands out as a pioneer of women’s writing in modern Croatian drama and theatre; therefore, she will be placed into the context of Croatian female dramatic production. The thesis presents her first book of dramatic texts – Pred vratima Hada, but the majority of this thesis is dedicated to plays A tek se vjenčali, Adagio, Posljednja karika and Giga i njezini, which are united in the author's second collection – Četiri drame. These plays show a unique dramatic world and all four of them are analyzed in the context of modern Croatian women's writing. The middle part is dedicated to postmodern elements in Četiri dame. Plays deal with female characters that are described through their family and matrimonial relationships. It also deals with postmodernist shaping of dramatic time and space which is realized through the interweaving of the real and the unreal and with intertextuality in Lada Kastelan’s plays since she used templates from Croatian literature in her plays Posljednja karika and Giga i njezini. The aforementioned elements are analyzed in all four plays and dealt with in separate chapters. The analysis shows that those elements, even though unrepeatable and shaped in a unique way in every play, are crucial in understanding the author's poetics and plays. The thesis confirms the importance of Lada Kastelan for modern dramatic production

    Identifying the Meaning of Kafa'ah in Marriage (Analysis of the Views of Community Leaders of Gampong Lada, Mutiara Timur Subdistrict, Pidie Regency): Identifikasi Makna Kafa’ah Dalam Perkawinan (Analisis Pandangan Tokoh Masyarakat Gampong Lada Kecamatan Mutiara Timur Kabupaten Pidie)

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    Kafa'ah is one of the important things in marriage, this is intended to produce harmony in the household so that it can be nurtured and the creation of a happy and harmonious household. Islam provides guidelines for choosing a partner based on four important criteria, namely religion, wealth, descent, and beauty. The different views were expressed by community leaders in Gampong Lada Village, namely expanding the meaning of kafa'ah in marriage by adding educational, position and job criteria in interpreting the meaning of kafa'ah. The problem in this thesis is how the meaning and criteria of kafa'ah in marriage and how the community leaders of Gampong Lada interpret kafa'ah and its criteria in marriage. The type of research used by the author in compiling this thesis is descriptive qualitative research. The results of this study indicate that the community leaders of Gampong Lada Village are very understanding about the meaning of kafa'ah in marriage, because the community leaders of Gampong Lada are very knowledgeable about religious knowledge. Gampong Lada community leaders interpret kafa'ah as equality, equivalence, and harmony between the prospective groom and the prospective bride when they want to get married. And the community leaders of Gampong Lada are of the view that for now the aspects of property, education, work, and position are very relevant in terms of kafa'ah, but even so, community leaders in Gampong Lada Village, Mutiara Timur District, and Pidie Regency do not rule out the religious aspect of the kafa issue. At this point, the community leaders of Gampong Pepper view that the religious aspect as the most important aspect of the meaning of kafa'ah in marriage

    Tanaman Lada di Langkat Abad XIX Sampai XX

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    The Thesis entitled "Pepper Plantations In Langkat in the century of XIX untill XX. This is a history of plantation studies related to the society economic life of people who cultivated pepper plants in the Langkat region. Research methods used in the implementation of research studies is a historical method, which consists of heuristics, criticism, interpretation, and historiography. In the heuristic stage, the author has collected data from colonial period books, reports, and archives. Furthermore, once the data is collected, it is verified through internal and external criticism, so that all data is assembled to form a past construction. Until the final stages of writing in the form of a thesis. The Researchs aims to describe how early pepper plants in Langkat, the pattern of pepper cultivation, peaks and decline of pepper plants in Langkat in the XIX century until the XX century. The geographical condition of the Langkat region became an important factor in the beginning of pepper cultivation. In addition, the distribution and access to land is not a problem, coupled with the relatively small production costs, so the capital required in this pepper planting business is not too large. In its development, although pepper cultivation efforts were initially less developed, the occurrence of international trade dynamics made the pepper commodity market share more attention. Interestingly, the high demand from the international market for pepper commodities, especially from the Malay peninsula, has made pepper entrepreneurs and farmers competing in planting and producing the crops. This then makes the pepper production rate has increased significantly. In addition, the development of pepper planting in Langkat indirectly also led to changes in demographic composition of the population until the emergence of new areas that have characteristic identity characteristic. In subsequent developments, the decline in pepper production in Langkat generally caused by various factors, such as: Falling demand due to high price conditions. This is also not separated from the condition of crop failure by farmers where pepper crops result damaged of pest attack. In addition, the condition is further compounded by the opening of East Sumatra region into a large onderneming economic region.Skripsi yang berjudul “Tanaman Lada Di Langkat Abad XIX Sampai XX ini adalah sebuah kajian sejarah perkebunan yang berhubungan dengan kehidupan sosialekonomi masyarakat yang membudidayakan tanaman lada di wilayah Langkat. Metode penelitian yang digunakan dalam pelaksanaan kajian penelitiannya adalah metode sejarah, yang terdiri dari heuristik, kritik, intepretasi, dan historiografi. Dalam tahap heuristik, penulis telah mengumpulkan data dari buku-buku, laporan, dan arsip-arsip masa kolonial. Selanjutnya pada saat setelah data terkumpul, kemudian diverifikasi melalui kritik intern dan ekstern, sehingga seluruh data dirangkai hingga membentuk suatu konstruksi masa lampau. Hingga pada tahap akhir dilakukan penulisan dalam bentuk skripsi. Penelitian ini bertujuan untuk mendeskripsikan bagaimana awal tanaman lada di Langkat, pola budidaya tanaman lada, puncak dan kemunduran tanaman lada di Langkat pada abad ke XIX sampai abad XX. Kondisi geografis wilayah Langkat menjadi faktor penting awalnya usaha penanaman lada. Selain daripada itu, persebaran dan akses terhadap lahan bukanlah sebuah permasalahan, ditambah lagi dengan biaya produksi yang relatif kecil, sehingga modal yang dibutuhkan dalam usaha penanaman lada ini tidak terlalu besar. Dalam perkembangannya, meskipun usaha penanaman lada awalnya kurang begitu berkembang, namun terjadinya dinamika perdagangan internasioanl membuat pangsa pasar komoditi lada mendapat perhatian lebih. Menariknya adalah permintaan yang tinggi dari pasar internasional akan komoditi lada terutama dari kawasan semenanjung Malaya, membuat para pengusaha dan petani lada berlombalomba dalam menanam dan memproduksi tanaman tersebut. Hal tersebut kemudian membuat angka produksi lada mengalami peningkatan yang cukup signifikan. Di samping itu, berkembangnya usaha penanaman lada di Langkat secara tidak langsung juga menyebabkan perubahan komposisi demografi penduduk hingga munculnya daerah-daerah baru yang memiliki karakteristik identitas yang khas. Dalam perkembangan selanjutnya, terjadinya penurunan angka produksi lada di Langkat umumnya disebakan berbagai faktor, seperti: Permintaan yang turun disebabkan kondisi harga yang cukup tinggi. Hal ini juga tidak terlepas dari kondisi gagal panen oleh petani dimana tanaman lada rusak akibat serangan hama. Selain itu, kondisi tersebut makin diperparah dengan dibukanya daerah Sumatera Timur menjadi kawasan ekonomi onderneming secara besar-besaran.Skripsi Sarjan

    Roman Timura Kibirova "Lada ili Radost'" : postkonceptualistskaâ idilliâ

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    In this article the „village” story of Timur Kibirov „Lada or joy”, is analyzed from the point of view of the poetics of post-conceptualism, one of the main representatives of which is Kibirov. A distinctive feature of post-conceptualism is combining the formal techniques of modernism (intertextuality, irony, pastiche) and sincere emotional tone of the author. In his first work of prose the poet uses three interrelated genres or trends – idyll, sentimental novel and village prose, to create a stylistic collage, in which parody, irony and idyllic mode do not contradict but complement each other

    Il ruolo di Igor Bestuzhev-lada nella progettazione istituzionale della previsione a Gorizia e in Italia. Omaggio a Igor Bestuzhev-Lada

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    This tribute to Igor Bestuzhev-Lada takes the form of an analysis of his relationship with Alberto Gasparini: two halves of a whole who carried out a number of initiatives concerning the prediction of the future. In this case, we are interested in those initiatives involving Bestuzhev-Lada which lead to the development of predictive studies in Italy, in particular at the ISIG in Gorizia and at the University of Trieste. As director of the ISIG and of the Department of Human Sciences at the University of Trieste, the author focuses on the efforts to build a network of relations with futurologists and to conduct research on futures studies. Joint initiatives with Bestuzhev-Lada were set up in Madrid, Moscow, Gorizia and Trieste (1990-1992), with exchanges of researchers between Italy and Moscow. The first events (congresses, conferences, summer schools, etc.) started dialogue and thus paved the way for the refoundation of the Italian journal “Futuribili” (1994). The third phase saw the foundation of the International Futures Researches Academy (1999-2000) based in Gorizia, which institutionalised the collaboration with Igor Bestuzhev-Lada and the Russian Futures Studies Academy of Moscow. This actually was a highly complex operation, involving the dissemination in international academic circles of the idea of social prediction, the gathering of support and enrolments, the formulation of initiatives, programmes and of the articles of constitution. The following years saw the launch of activities, first of all at the ISIG in Gorizia and at the RFSA in Moscow, and participation in events by members of IFRA. The activities included the publication of books and of the journal “Fututribili”, complementary research projects in Italy and Russia, joint international summer schools and the organisation of conferences and congresses.L’omaggio a Igor Bestuzhev-Lada è espresso attraverso il percorso delle relazioni tra lo stesso Bestuzhev-Lada e Alberto Gasparini svolte in Italia per descrivere, costruire e cumulare le azioni sviluppate da due metà di un tutto che riguarda la previsione del futuro. La previsione qui considerata riguarda il ruolo che ha giocato Bestuzhev-Lada nello sviluppo degli studi di previsione in Italia, e in particolare a Gorizia, all’ISIG e all’Università di Trieste. L’autore mette in risalto, nella sua qualità di direttore dell’ISIG e del Dipartimento di Scienze dell’Uomo dell’Università di Trieste, lo sforzo di costruire una rete di relazioni con futurologi e di svolgere ricerche sui futures studies. Si hanno iniziative comuni con Bestuzhev-Lada a Madrid, a Mosca, a Gorizia, a Trieste (1990-1992), con scambi di ricercatori dall’Italia a Mosca e viceversa. I primi eventi (convegni, Summer Schools, conferenze, ecc.) aprono il dialogo e quindi conseguentemente la strada a rifondare la rivista italiana “Futuribili” (1994). Una terza fase istituzionalizza la collaborazione con Igor Bestuzhev-Lada e con la “Russian Futures Studies Academy” di Mosca nella fondazione dell’“International Futures Researches Academy” (IFRA) nel 1999-2000, con sede a Gorizia. È un’operazione molto complessa, che vede la diffusione dell’idea per il mondo degli studiosi della previsione sociale, la raccolta delle adesioni, la formulazione delle azioni, dei programmi, e dello Statuto. Gli anni successivi vedono le attività messe in atto, anzitutto con l’ISIG e la RFSA di Mosca, ma anche la partecipazione agli eventi degli aderenti all’IFRA. Vengono così illustrate le attività che nascono: pubblicazione di libri e della rivista “Futuribili”, ricerche complementari in Italia e in Russia, Summer Schools internazionali condivise, organizzazione di Conferenze e di Convegni
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