44 research outputs found
Potensi Mangiferin dari Mangga (Mangifera indica Linn) dalam Pencegahan Relaps Pasca Perawatan Ortodonti (Kajian Ekspresi MMP 8, dan TGF β)
No full textLatar Belakang : Relaps merupakan masalah yang masih sering terjadi dan berakibat penurunan keberhasilan perawatan ortodonti. Remodeling tulang sangat membantu pada perawatan ortodonti, terutama untuk mencegah relaps hasil perawatan. Osteoblas dan osteoklas memiliki peranan yang sangat penting pada saat proses remodeling tulang. MMP-8 merupakan kolagenase yang dapat merusak substrat matrik ekstra seluler yaitu kolagen I, II, III yang berperan penting pada degradasi jaringan ligamen periodontal.Penarikan kemotaktik osteoblas atau prekursornya ke daerah defek resorbsi merupakan awal pembentukan tulang. Proses ini dimediasi oleh faktor lokal yang diproduksi selama proses resorpsi, salah satunya adalah TGF-β. Mangiferin memiliki efek imunomodulasi, menghambat pembentukan osteoklas dan resorpsi tulang melalui supresi aktivasi RANKL yang menginduksi NF-kB dan ERK. NF-kB telah terbukti memainkan peran sangat penting dalam osteoklastogenesis. Supresi aktivasi NF-kB akan menghambat pembentukan osteoklas.
Tujuan Penelitian : Membuktikan mangiferin (Mangifera indica Linn) berpotensi membantu proses remodeling tulang untuk mencegah relaps pasca perawatan ortodonti.
Metode Penelitian : Penelitian ini terdiri dari tahap deskriptif ekploratif dan penelitian kuantitatif. Mangiferin terstandarisasi dilakukan pembuatan hidrogel dengan konsentrasi 6.25% dan 12.5%.Sampel penelitian adalah tikus putih jantan galur wistar (Rattus novergicus) dengan umur 3-4 bulan dan memiliki bobot badan 200-250 gram. Dibagi dalam 3 kelompok : Kontrol negatif (tanpa diberi perlakuan), kontrol positif (dipasang nickel titanium closed coil tanpa aplikasi mangiferin), dan kelompok perlakuan (dipasang nickel titanium closed coil diberikan mangiferin aplikasi mangiferin dengan konsentrasi 6.25% dan 12.5 %). Pemasangan nickel titanium closed coil selama 10 hari dan kemudian dilepas. Setelah dilepas diaplikasikan hydrogel mangiferin 2x sehari selama 14 hari didekapitasi pada hari ke 1,3,5,7 dan 14.Media MHA digunakan untuk menguji daya absorbsi dan penetrasi mangiferin.Pengukuran viskositas dan pH mangiferin menggunakan viscometer Ostwald.Dilakukan pengukuran jarak biometrik gigi sebelum dan sesudah pelepasan alat. Pemeriksaan histologi dengan pewarnaan hematoxylin dan eosin. Pemeriksaan degradasi tulang dilakukan dengan Scanning Electron Microscope dianalisis dengan Energy Dispersive X-Ray Spectroscopy pemeriksaan reaktivitas protein MMP-8 dan TGF-β dengan Enzyme linked immunosorbent assay. Pelacakan MMP-8 dan TGF-β dengan ultraviolet-visible.
Hasil : Hasil uji One Way Anova menunjukkan mangiferin memiliki kemampuan daya absorbsi yang tinggi dan sangat dipengaruhi oleh konsentrasi dari mangiferin (p<0,01) dengan uji korelasi Pearson yang sangat kuat (r=0,98). Viskositas menentukan derajat absorbsi mangiferin untuk mempengaruhi remodeling tulang. Hasil uji Kruskall Wallis terlihat konsentrasi mangiferin memiliki pengaruh yang kuat terhadap perubahan jarak biometrik, dengan uji korelasi Spearman (r=0,8) dan terdapat perbedaan yang bermakna keduanya (p<0.05). Peningkatan osteoblas yang reaktif lebih tinggi dibandingkan dengan osteoklas menandakan terjadi remodeling tulang pasca perawatan ortodonti. Profil osteoblas dan osteoklas keduanya berbeda bermakna (p<0.05). Profil permukaan tulang mengalami kekompakan dan ditemukan sejumlah matrik tulang pada semua konsentrasi mangiferin. Peningkatan pembentukan matrik tulang sejalan dengan penurunan degradasi kalsium tulang. Penurunan protein MMP-8 dan peningkatan TGF –β. Protein MMP-8 terlacak pada panjang gelombang 250-300 nm. Sedangkan TGF-β pada panjang gelombang 200-245 nm
Kesimpulan : Mangiferin memiliki daya absorbsi dan viscositas yang baik sehingga efektivitas dalam mengkontrol produksi osteoblas dan osteoklas, mencegah degradasi kalsium, meningkatkan pembentukan matrik tulang. Mangiferin memiliki kemampuan terhadap peningkatan ekspresi MMP-8 dan peningkatan TGF-β sehingga berpotensi merangsang pembentukan tulang baru lebih cepat pada proses remodeling tulang untuk mencegah relaps pasca perawatan ortodonti.Background: Relapse is still a common problem causing the decrease in success rate of orthodontic treatment. Bone remodeling is essential in orthodontic treatment, especially in preventing relapse in treatment results. Osteoblasts and osteoclasts have an important role in bone remodeling. MMP-8 is a collagenase destructive to extracellular matrix substrate, i.e. collagen I, II, III, playing important role in the degradation of periodontal ligament tissues. The chemotacticalattraction of osteoblast or its precursors to the resorption defect area is the beginning of bone formation. This process is mediated by local factors released during the resorption process, one of which is TGF-β.Mangiferin has an immunomodulating effect, inhibits osteoclast formation and bone resorption through suppression of RANKL activation which induces NF-κB and ERK. NF-κB has been shown to play a very important role in osteoclastogenesis. Suppression of NF-kB activation will inhibit osteoclast formation.
Objectives :To prove the potential of mangiferin (Mangifera indicaLinn) assisting bone remodeling process in preventing relapse after orthodontic treatment.
Methods:This research consists of descriptive explorative phase and quantitative research. Standardized mangiferin was made hydrogel with a concentration of 6.25% and 12.5%. The sample of this research is Wistar strain male rats (Rattus novergicus) with 3-4 months of age and 200-250 gram body weight. Divided into 3 groups: Negative control (without treatment), positive control (mounted nickel titanium closed coil without the application of mangiferin), and treatment group (mounted nickel titanium closed coil were given mangiferin with mangiferin applications with concentrations of 6.25% and 12.5%). Nickel titanium closed coil installation for 10 days and then removed. After being released, mangiferin hydrogel was applied 2x a day for 14 days, decapitated on days 1.3.5.7 and 14. MHA media was used to test the absorption and penetration of mangiferin. Measurement of viscosity and pH of mangiferin using the Ostwald viscometer. Biometric distances were measured before and after the removal of the tool. Histological examination with hematoxylin and eosin staining. Examination of bone degradation is done by Scanning Electron Microscope analyzed with Energy Dispersive X-Ray Spectroscopy Examination of MMP-8 Protein Reactivity and TGF-β with Enzyme linked immunosorbent assay. MMP-8 and TGF-β tracking with ultraviolet-visible.
Results: One Way Anova test results showed that mangiferin has a high absorption ability and is strongly influenced by the concentration of mangiferin (p <0.01) with the Pearson correlation test which is very strong (r = 0.98). Viscosity determines the degree of absorption of mangiferin to affect bone remodeling. Kruskall Wallis test results showed that the concentration of mangiferin had a strong influence on changes in biometric distances, with the Spearman correlation test (r = 0.8) and there were significant differences in both (p <0.05). Increased reactive osteoblasts higher than osteoclasts indicate bone remodeling after orthodontic treatment. Both osteoblasts and osteoclasts were significantly different (p <0.05). The bone surface profile is compacted and a number of bone matrices are found at all mangiferin concentrations. Increased bone matrix formation is in line with decreased bone calcium degradation. Increased MMP-8 protein and TGF -β. MMP-8 proteins are tracked at wavelengths of 250-300 nm. Whereas TGF-β at wavelengths of 200-245 nm.
Conclusion: mangiferin has good absorption and viscosity power so that it is effective in controlling osteoblast and osteoclast production, preventing calcium degradation, increasing bone matrix formation. Mangiferin has the ability to increase expression of MMP-8 and increase TGF-β so that it has the potential to stimulate new bone formation more quickly in the process of bone remodeling to prevent relapse after orthodontic treatment.209 HalamanDisertasi Dokto
Nanosilika dari Sekam Padi sebagai Penguat Lapisan Opak pada Retainer Gigi Tiruan Jembatan Keramik-Logam
No full textRice husks are organic waste that their utilization remains not optimum in Indonesia.
Given that a relatively high silica content within the waste, then these husks have a
potential usage to be source of silica as a reinforcing material for opaque porcelain
powder in Metal-Ceramic retainer of Fixed Partial Dental Prostheses (FPDs). This
study aims to use the nanosilica synthesized from rice husks as a reinforcer on opaque
porcelain powder in the preparation of metal-ceramic samples. The nanosilica was
synthesized from rice husks via sol-gel methods with the presence of 1N HCl and the
sonication process with ultrasonic homogenizer for 270 minutes. The nanosilica was
then used as the reinforcer into the commercial opaque porcelain powder with variation
of mixture i.e., 0.5%; 1%; 1.5%; 2%; 2.5%; 3% and without mixing. Furthermore,
morphological characterizations were carried out through SEM, TEM and PSA, and
the physico-chemical properties were determined through FT-IR, XRD, XRF and
SEM- EDX instrument. A samples of Co-Cr metal measured with dimension of 25 x
3 x 0.5 mm was prepared to 140 samples. The veneering of opaque and dentine
porcelain with dimension of 8 x 3 x 1.1 mm on metal samples were carried out via
manually-handed lay out method, and then the coated metal samples were placed for
sintering treatment at 920-950°C. The mechanical properties were characterized via
flexural, compression, shear, and hardness tests. The FT-IR results confirmed the
presence of silica in the form of siloxane, silanol and xiloxy groups at wavenumbers
of 1063 cm-1, 960 cm-1, and 793 cm-1 respectively; the XRD results showed 2θ of silica
with an angle of 22.7° amorphous structure; XRF results obtain pure silica of
99.50%; while the PSA results show a nanosilica particle size of 28.0 ± 9.6 nm; SEM
and TEM result depicted spherical shapes that agglomerate with one to another to form
clusters; and the SEM-EDX results confirmed the highest amount of silica in sample
with 1% of ratio. The results of statistical analysis showed a significant effect on
mechanical properties of metal-ceramic samples through the mixing of nanosilica with
ratio 0.5%; 1% and 1.5% (p<0.05). The nanosilica can be synthesized from rice husks
via sol-gel and sonication methods, then mixing with opaque porcelain powder can
increase the mechanical properties of metal-ceramic samples.137 HalamanDisertasi Dokto
Mekanisme Ekstrak Etanol dan N-Heksan Daun Pirdot (Saurauia vulcani Korth.) sebagai Analgetika – Antiinflamasi pada Pergerakan Gigi Ortodonti terhadap Tikus Wistar (Rattus norvegicus)
Full text linkAn orthodontic pain due to aseptic inflammation came from orthodontic
mechanics. NSAIDs and steroids are some options to reduce pain even though it has
a negative effect. Secondary metabolites of Saurauia vulcani Korth. has an
analgetic and anti-inflammatory potential. Aims, observing the analgetic and antiinflammatory
mechanism of ethanol and n-hexane extract and their effect on the
quality of orthodontic tooth movement. Research methods: The experiment with
controls was carried out in three stages; from a phytochemistry study of the ethanol
extract of pirdot leaves (EEDP) and n-hexane extract of pirtdot leaves (EnHDP);
The next stage was in-vivo study which analyzed the effectiveness of EEDP and
EnHDP analgetic with paracetamol as control and anti-inflammatory potential to
body with natrium diclofenac as control, the third stage was for orthodontic tooth
movement both with paracetamol and natirum diclofenac as controls. Groups were
based on the dosage of EEDP and EnHDP, started from 50-400mg/kgBW and the
groups were reduced based on the effectiveness dosage at each level of the study.
The third stage was to analyze the effectiveness both extracts in analgetics, antiinflammatories
and their effects on orthodontic tooth movement with the closed
coil spring force from the first molar to the insicors. OTM study were analyzed the
proinflammatory cytokine (TNF-α), neuropeptide (Substance-P), cortisone
hormone, and the alveolar maxillae histological study was in hematoxylin-eosin
and immunohistochemistry staining (Tartrate-Resistant Acid Phosphatase-5
(TRAP5) dan Bone Morphogenetic Protein 9 (BMP9)) for cellulars quantification.
Result, EEDP’s secondary metabolites were flavonoids, saponins, tannins,
glycosides, and steroids/triterpenoids; EnHDP’s was steroid/triterpenoid. Total
Phenol and Flavonoid Content (TPC and TFC) were higher for EEDP than
EnHDP. The effectiveness from the writhing test showed the best analgetic efficacy
at EEDP 200gr/kgBW group (91,12%). The phase 1 of formalin test was best in
EnHDP 400gr/kgBW group (97.62%) and EEDP 200gr/kgBW group (100%) at
phase 2. The AUC’s value of edema test was below the control group at the EEDP
group and it coincidentally found for the plantar rats’ histological analysis. Followup
studies of analgesics on orthodontic force showed that both pirdot leaf extracts
can reduce the TNF- α, the substance-P, and the cortisone levels. Furthermore,
EEDP and EnHDP also accelerated the tooth movement, which supported by an
increase of the osteoclast numbers, but only EEDP has better effect to osteoblast
growth. Conclusion: EEDP and EnHDP provided analgetic and anti-inflammatory
effects through several in-vivo preliminary and OTM tests in this study, it also
enhanced rate of the tooth movement.183 HalamanDisertasi Dokto
Inventory, Konservasi, dan Pengembangan Potensi SertaManfaat Tumbuhan Sumatera Secara Berkesinambungan IV;Penelitian Kimia Tumbuhan Rendah Sumatera
No full text
Kajian Kimia Dari Fraksi Etil Asetat Kulit Batang Kayu Pahit (Picrasma Javanica Bl.)
No full text Senyawa utama N-1 dari fraksi etil asetat kulit batang kayu pahit (Picrasma Javanica Bl) telah diisolasi dan dikarakterisasi dengan kromatografi kolom dan dilanjutkan dengan kromatografi preparatif. elusidasi struktur dan analisis data spektrum yang digunakan ultraviolet (UV) spektroskopi, infra merah (IR), 1H RMI (Resonansi Magnet Inti), 13C RMI, Massa, COSY (Correlated Spectroscopy), HSQC (Heteronuclear Single Quantum Correlation), HMBC (Heteronuclear Multiple Bond Correlation) dan studi literatur menunjukkan bahwa senyawa yang isolasi adalah Javanicin A. </jats:p
Effect of Pharmacist Presence to Pharmaceutical Service at Pharmacies of Padang City, Indonesia)
No full textStandar pelayanan kefarmasian merupakan tolok ukur yang digunakan khususnya apoteker dalam melaksanakan pelayanan kefarmasian. Penelitian ini bertujuan melihat pengaruh kehadiran apoteker terhadap pelayanan kefarmasian, bersifat cross sectional menggunakan kuisioner bulan Febuari – Agustus 2015. Sampel penelitian 90 apoteker di Apotek diambil secara random. Hasil penelitian menunjukkan bahwa Persentase kehadiran apoteker di apotek kota Padang sebesar 58,67% dan Pelayanan Kefarmasian dikategorikan kurang (pemeriksaan resep 57,06%, penyiapan resep 55,12%, dan penyerahan resep 51,89%) dengan persentase rata-rata 54,69%. Hasil pengujian analisis regresi linear dapat diketahui pengaruh X terhadap Y positif. Pengujian Koefisien Product moment menunjukkan hubungan X terhadap Y kuat dimana : r=0,910, dalam pengujian koefisen determinasi menunjukkan bahwa X mempunyai pengaruh tinggi terhadap Y yaitu sebesar 82,8% dan 17,2% dipengaruhi oleh faktor lain. Berdasarkan tabel uji F test sebesar 422.579 dengan signifikansi 0,000. Karena tingkat signifikan < 0,05, maka dapat disimpulkan bahwa variabel Kehadiran Apoteker mempunyai pengaruh terhadap Pelayanan Kefarmasian.</jats:p
Analisis faktor-faktor yang mempengaruhi pilihan karir mahasiswa farmasi di Universitas Andalas
Full text linkBased on PP 51 of the year 2009, the pharmaceutical practices have very of choice of carreer-field. The purpose of this research is to determine the most important factor that influence the career choices and the carrier priority choosing of pharmacy students. It was a descriptive study which conducted by survey method by using stratified random sampling. The respondents are 85 students, consisting of 1st, 2nd, 3rd and 4th grade students from Faculty of Pharmacy, Andalas University. The results shown that, in term of the profile of respondent, known that the most of the students choices to work at Pharmaceutical Industry, Hospital, University/Research Institute, pharmacy, pharmacy retail and others career-field (such as BPOM, civil servant at ministry of health and enterpreneur) of 48.2%, 35.3 %, 4.7%, 3.5 % 2.4 % and 5.9%, respectively. Based on the descriptive analysis, it was described that work field was the most important criteria in choosing their future career. The others important criterias those also afforded as the affecting factors were geographis, payment/income, flexibility of work-schedule, possibility of career-development and benefit, successively. The results showed that the independent sample t-test in term of gender (i.e. men and women) showed the same three main affecting factors for choosing the career-field (including field of work, geographis as well as payment/income). However, 4th, 5th and 6th factors were showed the different results. In this researh, the work field is the most important for the respondents to decide their future choice and the carrier priority choosing from the respondents is to work in the Pharmaceutical Industry
3,4-DIHYDROXYBENZOIC ACID AND 3,4-DIHYDROXYBENZALDEHYDE FROM THE FERN <i>Trichomanes chinense</i> L.; ISOLATION, ANTIMICROBIAL AND ANTIOXIDANT PROPERTIES
Full text link3,4-dihydroxybenzoic acid (1) and 3,4-dihydroxybenzaldehyde (2) have been isolated from ethyl acetate fraction of methanolic fractions of leaves, stems and roots of the fern Trichomanes chinense L. (Hymenophyllaceae). These two compounds also showed significant antioxidant using DPPH and antimicrobial activities using the disc diffusion assay
Kajian Fitokimia Fraksi Etil Asetat dari Lichen Stereocaulon massartianum Hue. dan Uji Aktivitas Antibakteri dengan Metode KLT-Bioautografi
Full text linkThis research is a continuation study in the inventory of lichens of the genus Stereocaulon which is currently
focused on the Stereocaulon massartianum Hue collected in the rocks of Diatas Lake, West Sumatera, Indonesia. Reports on phytochemical studies and pharmacological activities of this species are still limited, based on literature studies that have been carried out. This research was conducted to determine their secondary metabolites and potential antibacterial activity. The airdried thallus of lichen S. massartianum was macerated successively using n-hexane, ethyl acetate and methanol solvents. The compounds were separated by chromatography and recrystallization methods, then analyzed by spectroscopy (UV-Vis, FTIR, 1 Hand 13C-NMR). Furthermore, the antibacterial activity assay was performed by agar diffusion method on ethyl acetate extract, and TLC-Bioautography for the isolated compound. Three compounds have been isolated from ethyl acetate extract, i.e. atranorin (1), stictic acid (2) and norstictic acid (3). The results of antibacterial assay from the extract showed antagonistic activity against pathogenic bacteria S. aureus, E. faecalis, E. coli, and P. aeruginosa at concentrations of 10 and 20%, while TLC-bioautography of
compound 3 exhibited growth inhibition area in all test bacteria
