288,824 research outputs found
Anthropometric and physiological changes in elite female water polo players during a training year
No full textThe aim of the current study was to monitor physiological and anthropometric characteristics of elite female water polo players within the periodized training year. Fourteen subjects participated in the current study. However, only six subjects (Mean ± SD: Age 22.8 ± 3.7 years, Height 171.0 ± 10.8 cm, Body Mass 66.3 ± 4.7 kg) completed all sessions and thus were used for subsequent analyses. Subjects undertook testing in the general preparation, specific preparation and competition phases, with the final session being during the peaking component of the competition phase. Laboratory-based physiological measurements comprised peak oxygen uptake, anaerobic power, leg power, strength and flexibility, while anthropometric measurements included body fat percentage. Sport-specific tests involved the Multistage Swimming Shuttle Test (MSST) and the 30-second crossbar jumps. A repeated measures ANOVA revealed significant differences between testing sessions for body mass (F3,15 = 4.025, P = 0.028), body fat (F3,15 = 9.194, P = 0.001), MSST (F3,15 = 5.050, P = 0.017) and crossbar jumps (F2,10= 16.034, P = 0.001). No statistically significant differences were found for any other variables. The results of the study suggest that changes in anthropometric characteristics and performance parameters of elite female water polo players over a periodized training year occur with no changes in laboratory-based physiological measurements
Comparison of two anaerobic water polo-specific tests with the Wingate test
No full textThe purpose of the current study was to compare 2 water polo–specific tests—the 14 3 25-m swims (SWIM) and the 30-second crossbar jumps (30CJ)—with a laboratory-based test of anaerobic power, the Wingate Anaerobic Test (WAnT). Thirteen elite women’s water polo players (mean 6 SD: age 22.0 6 4.4 years, height 168.7 6 7.9 cm, body mass 65.9 6 6.1 kg, body fat 23.6 6 3.5 %, maximum oxygen uptake 51.4 6 4.5 mlkg21min21) participated in the study. The SWIM involved 14 repeated ‘‘all-out’’ sprints every 30 seconds. Swimming time was recorded, and sprint velocity, mean velocity (Vmean), and the gradient of the linear regression equation (GRADIENT) were calculated. The 30CJ involved repeated in-water water polo jumps and touching the goal crossbar with both hands. The number of touches in 30 seconds was recorded. Additionally, the subjects completed a 30-second WAnT, and mean power (Mp) and fatigue index (FI) were calculated. Kendall tau (t) rank correlation was used to examine for correlation between ranks. Significance level was set at p # 0.05. No significant correlation was found between any of the measures of the WAnT and the 2 sport-specific tests. It was suggested that the WAnT may not be an appropriate evaluation tool for anaerobic power assessment of water polo players, stressing the importance of sport-specific tests
STIL binding to Polo-box 3 of PLK4 regulates centriole duplication
No full textPolo-like kinases (PLK) are eukaryotic regulators of cell cycle progression, mitosis and cytokinesis; PLK4 is a master regulator of centriole duplication. Here, we demonstrate that the SCL/TAL1 interrupting locus (STIL) protein interacts via its coiled-coil region (STIL-CC) with PLK4 in vivo. STIL-CC is the first identified interaction partner of Polo-box 3 (PB3) of PLK4 and also uses a secondary interaction site in the PLK4 L1 region. Structure determination of free PLK4-PB3 and its STIL-CC complex via NMR and crystallography reveals a novel mode of Polo-box-peptide interaction mimicking coiled-coil formation. In vivo analysis of structure-guided STIL mutants reveals distinct binding modes to PLK4-PB3 and L1, as well as interplay of STIL oligomerization with PLK4 binding. We suggest that the STIL-CC/PLK4 interaction mediates PLK4 activation as well as stabilization of centriolar PLK4 and plays a key role in centriole duplication
Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
No full textWe have investigated the function of mitotic kinesin-like protein (MKlp) 2, a kinesin localized to the central spindle, and demonstrate that its depletion results in a failure of cleavage furrow ingression and cytokinesis, and disrupts localization of polo-like kinase 1 (Plk1). MKlp2 is a target for Plk1, and phosphorylated MKlp2 binds to the polo box domain of Plk1. Plk1 also binds directly to microtubules and targets to the central spindle via its polo box domain, and this interaction controls the activity of Plk1 toward MKlp2. An antibody to the neck region of MKlp2 that prevents phosphorylation of MKlp2 by Plk1 causes a cytokinesis defect when introduced into cells. We propose that phosphorylation of MKlp2 by Plk1 is necessary for the spatial restriction of Plk1 to the central spindle during anaphase and telophase, and the complex of these two proteins is required for cytokinesis
COORDENADOR DE POLO: UMA DISCUSSÃO ACERCA DOS CONHECIMENTOS NECESSÁRIOS À UMA BOA GESTÃO
No full textO presente trabalho tem como objetivo apresentar e discutir uma série de competências complementares às exigidas legalmente que auxiliarão a figura do Coordenador de Polo no processo de gerenciamento de um Polo de Apoio Presencial. Entende-se que, apenas com os pré-requisitos mínimos exigidos por lei, que privilegiam os aspectos acadêmicos em detrimento de conhecimentos de práticas de gestão, os Coordenadores de Polo podem não se mostrar preparados para o exercício de suas funções. Desta forma, num cenário de expressivo crescimento de cursos de Educação a Distância (EaD), não tendo as competências técnicas necessárias, os coordenadores de Polos poderão não suprir as necessidades do Polo, conseqüentemente, não atenderão as expectativas, demandas e necessidades dos alunos, professores e da própria comunidade. Sugere-se, então, alguns processos de gestão que poderão auxiliá-los em suas tarefas, a saber: gestão estratégica, gestão da infra-estrutura, gestão de pessoas, gestão de processos, gestão de marketing. Os pré-requisitos básicos para a contratação de um coordenador de Polo não exigem, a certificação dos conhecimentos nas áreas de gestão, mas a necessidade imposta pelo meio, cada vez mais requer tais conhecimentos
O polo de apoio presencial e o desenvolvimento socioeconômico: uma leitura do entorno
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia e Gestão do Conhecimento, Florianópolis, 2015.A expansão da Educação a Distância no Brasil acontece de forma constante. Através de sua principal característica, na qual professores e alunos estão separados no tempo e no espaço físico, torna-se necessária a criação dos polos de apoio presencial. Com a implantação dos polos, além da oportunidade de acesso ao ensino superior, percebe-se a promoção do desenvolvimento socioeconômico na comunidade em que o mesmo foi implantado. Nesse sentido, esta dissertação tem como objetivo identificar os fatores de transformação no desenvolvimento socioeconômico provocado no entorno do polo de apoio presencial após sua implantação. Para a realização da pesquisa, quanto aos objetivos, classificou-se como descritiva e exploratória. A abordagem adotada para a análise dos dados foi a qualitativa e quantitativa e, para a coleta de dados foi realizado levantamento bibliográfico, documental e aplicação de questionário. O método de análise de dados da pesquisa foi o dedutivo. Os participantes da pesquisa foram coordenadores de polo de apoio presencial do Estado de Santa Catarina que oferecem cursos na modalidade a distância através do Sistema UAB. Após a organização dos dados coletados, os mesmos foram analisados de forma dedutiva, no qual foi possível observar que, independente do índice de desenvolvimento humano do município as organizações produtivas locais sofreram algum tipo de mudança após a implantação do polo. Constatou-se que fatores como o acesso ao ensino superior público, a oportunidade de formação profissional e o crescimento econômico ocasionados após a presença do polo, refletiram no desenvolvimento socioeconômico do seu entorno. Por fim, considera-se que a disseminação do conhecimento, ocasionada pela implantação do polo de apoio presencial, contribui para que a comunidade local adquira um novo olhar sobre o polo, reconhecendo este como elemento que contribui para o desenvolvimento socioeconômico local.Abstract : The expansion of the Distance Education in Brazil happens steadily. Through its main feature, in which teachers and students are separated in time and physical space, it is necessary to create the ad hoc support poles. With the implementation of the poles, and the opportunity to access to higher education, the promotion of socio-economic development is perceived in the community in which it was implemented. In this sense, this work aims to identify the factors of transformation in the socioeconomic development caused surrounding the presence support pole after its implementation. For the research, the aims, was classified as descriptive and exploratory. The approach adopted for the analysis of data was qualitative and quantitative, and for data collection was performed literature review, document and questionnaire. The method of research data analysis was deductive. Survey participants were engineers on-site support pole of the State of Santa Catarina offering courses in the distance through the UAB System. After the organization of the data collected, they were analyzed as deductions, in which it was observed that, regardless of the human development index of the municipality local productive organizations experienced some sort of change after the implementation of polo. It was found that factors such as access to public higher education, vocational training opportunities and caused economic growth after the presence of polo, reflected in socio-economic development of their surroundings. Finally, it is considered that the dissemination of knowledge, caused by the deployment of classroom support pole, contributes to the local community acquires a new look at the pole, recognizing this as an element that contributes to local socio-economic development
Marco Polo: the character, the person, the narrator
No full textopenL’indagine organizzata in tre capitoli ha sviluppato il legame tra Marco Polo e il Devisement du monde e la cultura contemporanea. A questo macro-tema se ne aggiungono altri volti ad illuminare ogni zona d’ombra di una tematica apparentemente enorme. L’avvio della discussione si apre con una necessaria visione storica della persona Marco Polo. All’interno del primo capitolo si troverà un’attenta analisi del legame tra storia e realtà contemporanea, predisponendo delle "vite potenziali" di Marco Polo. Questo avvio mostrerà dunque le caratteristiche attribuite nella società contemporanea a Marco. Il secondo capitolo introdurrà il Devisement du monde in un’analisi di alcune tematiche d’interesse riguardanti il carattere dell’opera: il meraviglioso, lo “straniamento”, la sua funzione. A seguito di queste caratteristiche “biografiche” dell’opera, esattamente come nel precedente capitolo, si è predisposta una ricostruzione “reale”, filologica, dell’opera seguita da una costruzione potenziale, "popuar". Compresi dunque i legami tra Marco Polo, la sua opera e la nostra cultura contemporanea, compresa dunque complessivamente l’essenzialità del tutto marcopoliano, attuato a più riprese in modi artistici differenti, è stato necessario indagare all’interno del Devisement du monde le motivazioni di questa visione a tratti distorta, a tratti “separata” -tra personaggio, persona e narratore- rappresentata a più riprese nelle opere contemporanee. Il terzo ed ultimo capitolo, dunque, si predispone come il più innovativo, amministrato da un’interpretazione dell’opera che pone le sue basi appunto da una tripartizione intrinseca nell’opera: “Polo narratore”, “Polo persona”, “Polo personaggio”. La tripartizione verte a costituire dei poli funzionali che si diversificano, e, come definito nell’introduzione al capitolo, non definiscono solamente la figura di Marco, la quale certamente però è di rilievo. La suddivisione triadica delle funzioni risulterà ben visibile e seguirà un’altra tripartizione del testo, quella tematica: I. L’iniziazione, dove si osserva un Polo personaggio in crescita costante affidato all’insegnamento dei parenti Niccolò e Matteo oltre al costituirsi della sua vocazione “adamica”; II. Il viaggio, dove Polo personaggio (diviso tra Matteo-Niccolò-Marco) vedrà la sua totale formazione, amplificando i propri interessi, avvicinandosi di più a corte e permettendo al Polo narratore una narrazione più proficua e dettagliata; III. Il Gran Khan, dove Polo personaggio viene qui impersonato da Kublai. Kublai sarà diverso nella fisionomia, nell’agire ma le caratteristiche assegnateli saranno complementari a quelle di Marco, il Polo personaggio è mantenuto nella sua essenza e Marco e Kublai rappresentano i due mondi simili ma destinati ad essere divisi. Infine alla partizione triadica se ne potrebbe aggiungere un’ultima, la quale però, non crea un gruppo a sé a causa di un recupero vario e indistinto di ciò che in I, II e III era già stato osservato, terminando però l’opera solamente con una parziale vittoria della missione adamica. La conclusione definitiva è dunque simile al termine di un lungo viaggio, l’attraversare la cultura contemporanea ha dimostrato la particolare presenza di un Marco Polo diverso per ogni opera, sottolineandone però alcune caratteristiche rivedibili poi in un’attenta analisi dell’opera, non solo caratteriale ma anche funzionale.The investigation organised in three chapters developed the link between Marco Polo and the Devisement du monde and contemporary culture. This macro-theme is supplemented by others aimed at illuminating every grey area of an apparently enormous subject. The discussion opens with a necessary historical view of the person Marco Polo, referring to some biographical essays on the Venetian. Within the first chapter, subdivided into sub-chapters, one will find a careful analysis of the link between history and contemporary reality, viewing each phase of Marco Polo's life in the adaptations made by writers, filmmakers or cartoonists in contemporary reality. The chapter thus follows in parallel the 'real', historical reconstruction and the construction of a potential life of Marco Polo. This start will therefore show the characteristics attributed in contemporary society to Marco, a young man in the making who becomes the wise and strong connoisseur of the other, of the different.
The second chapter will introduce the Devisement du monde in an analysis of certain themes of interest concerning the work's character: the marvellous, the 'estrangement', its function. Following these 'biographical' characteristics of the work, exactly as in the previous chapter, a 'real', philological reconstruction of the work has been prepared, followed by a potential construction, where the characters previously observed are not distorted but functionalised for a contemporary narration in films, TV series or narrative texts.
Having thus understood the links between Marco Polo, his work and our contemporary culture, having thus comprehensively understood the essentiality of the whole of Marco Polo, implemented on several occasions in different artistic ways, it was necessary to investigate within the Devisement du monde the motivations for this sometimes distorted, sometimes 'separate' vision -between character, person and narrator- represented on several occasions in contemporary works.
The third and final chapter, therefore, sets itself up as the most innovative, administered by an interpretation of the work that is based precisely on an intrinsic tripartition in the work: "Polo narrator", "Polo person", "Polo character". The tripartition aims at constituting functional poles that diversify, and, as defined in the introduction to the chapter, do not only define the figure of Marco, which is certainly prominent. The triadic subdivision of functions will be clearly visible and will follow another tripartition of the text, the thematic one: I. The initiation, where we observe a constantly growing Polo character entrusted to the teaching of his relatives Niccolò and Matteo as well as the constitution of his "adamic" vocation; II. The journey, where Polo the character (divided between Matteo-Niccolò-Marco) will see his total formation, amplifying his interests, getting closer to the court and allowing Polo the narrator a more fruitful and detailed narration; III. The Great Khan, where Polo is here impersonated by Kubilai. Kubilai will be different in physiognomy, in acting but the characteristics assigned to him will be complementary to those of Marco, the Polo character is maintained in his essence and Marco and Kubilai represent the two worlds that are similar but destined to be divided. Finally, a last partition could be added to the triadic partition, which, however, does not create a group in its own right due to a varied and indistinct recovery of what had already been observed in I, II and III, only ending the work with a partial victory of the Adamic mission.
The final conclusion is therefore similar to the end of a long journey, the traversal of contemporary culture has shown the particular presence of a different Marco Polo in each work, emphasising, however, certain characteristics that can later be revised in a careful analysis of the work, not only characteristically but also functionally
Polo like kinase 2 tumour suppressor and cancer biomarker: new perspectives on drug sensitivity/resistance in ovarian cancer
No full textThe polo-like kinase PLK2 has recently been identified as a potential theranostic marker in the management of chemotherapy sensitive cancers. The methylation status of the PLK2 CpG island varies with sensitivity to paclitaxel and platinum in ovarian cancer cell lines. Importantly, extrapolation of these in vitro data to the clinical setting confirms that the methylation status of the PLK2 CpG island predicts outcomes in patients treated with carboplatin and paclitaxel chemotherapy. A second cell cycle regulator, p57Kip2, is also subject to epigenetic silencing in carboplatin resistance in vitro and in vivo, emphasising that cell cycle regulators are important determinants of sensitivity to chemotherapeutic agents and providing insights into the phenomenon of collateral drug sensitivity in oncology. Understanding the mechanistic basis and identification of robust biomarkers to predict collateral sensitivity may inform optimal use of chemotherapy in patients receiving multiple lines of treatment
Polo-like Kinase 1 Inhibition in <i>KRAS</i>-Mutated Metastatic Colorectal Cancer
No full textSummary:
Inhibition of Polo-like kinase 1 (Plk1) is a promising new target and therapeutic strategy in metastatic colorectal cancer, especially those with KRAS mutations. New data support further development of onvansertib, and highlights the role of circulating tumor DNA in phase I clinical trials.
See related article by Ahn et al., p. 2039</p
Studio di fattibilità per polo industriale aziendale
No full textElaborazione di uno strumento di valutazione preliminare circa la fattibilità tecnica e procedurale di un intervento di riorganizzazione e potenziamento del polo industriale "San Leonardo" di Parma. Strumento concepito come propedeutico alla programmazione di successive fasi di progettazione costruzione e gestione dell'opera al fine di garantire gli strumenti operativi e metodologici per la programmazione di un intervento di qualità e tecnicamente valido, nel rispetto del miglior rapporto fra i benefici e i costi globali di costruzione, manutenzione e gestione
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