1,721,681 research outputs found
Aspetti medico-legali del gioco del rugby
No full textil gico del rugby è considerato altamente pericoloso per gli aspetti traumatici, ma alcune lesioni possono caratterizzare lo sport in questione
PRODUZIONE DI LINEE TRASFORMATE DI ARABIDOPSIS CHE SOVRAESPRIMONO UN GENE DI ORZO INDUCIBILE DA STRESS
No full textUniformità ed obiettività nell'inquadramento medico-legale del danno psichico: una proposta metodologica
No full textIl danno psichico rappresente un aspetto del tutto peculiare in merito al suo rilevamento ed accertamento
PHYSIOLOGICAL ANALYSIS OF YEAST SACCHAROMYCES CEREVISIAE CELLS BY FLOW CYTOMETRY DURING EXPOSURE TO FURFURAL, AN IMPORTANT FERMENTATION INHIBITOR
No full textSaccharomyces cerevisiae is the preferred microorganism in the ethanol fermentation industry from
renewable resources such as lignocellulosic biomass. In the process of obtaining monomeric sugars
from lignocellulose trough a combination of physical, chemical and enzymatic treatments of biomass
formation of inhibitory compounds occurs among wich furfural is the most abundant (Almeida et al,
2007). S. cerevisiae is relatively tolerant to furfural because it is capable of converting it to the
corresponding alcohol with lower inhibitory capability by NAD(P)H dependent reactions
(Taherzadeh et al, 1999). Unfortunately these mechanisms of detoxification compete for key
enzymes and cofactors needed to bra nch carbon flow to respiration and to ethanol production.
Several studies have reported that furfural decreased yeast viability, specific growth rate and
volumetric fermentation rate. A recent work (Allen et al, 2010) has shown that furfural induced
reactive oxygen species (ROS) accumulation and cellular damage in S. cerevisiae. However the toxicity mechanism of furfural on yeast cells is not yet fully understood.
In our study we have conducted a flow cytometry analysis on yeast cells from CEN-PK family in
response to treatment with different concentrations of furfural. Exposure to high levels of inhibitor
did not caused ROS accumulation but resulted in increased intracellular depolarization and
accumulation of dead cells. These results may shed light on the mechanism of toxicity of furfural in
yeast and might be useful to improve the inhibitor resistance in S. cerevisiae
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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