6,100 research outputs found
Erratum to: Proximal humeral fractures in elderly patients (Aging Clinical and Experimental Research, (2013), 25, S1, (85-87), 10.1007/s40520-013-0078-x)
No full textIn the original publication of the article, the name of the third author was incorrectly published as C. Capra. However, the correct name should read as P. Capra
L'Anatolia / G. Capra
No full textL'Anatolia / G. Capra
Torino : Pubblicazione dell'Italica gens, 1920
139 p. : ill. ; 22 cm
Tit. della cop
A first glance on the epigenome of Capra hircus
No full textDNA methylation and microRNAs (miRNA) are two important forms of epigenetic modifications that play an important role in gene regulation in animals. Methylation at the carbon 5 position of cytosine residues is a fundamental layer of cellular differentiation through the control of transcriptional potential. MiRNA are small noncoding RNA molecules that regulate gene expression. Complete DNA methylomes for several organisms are now available; at the present, methylome of the domestic goat is unexplored. There is also still limited knowledge about miRNAs expression profiles in small ruminant species. Therefore, to contribute information on epigenetic modification in Capra hircus, we analysed the methylome and the miRNA population of three tissues (hypothalamus, pituitary and ovary) from 3 adult Saanen goats. We used Methylated DNA binding domain sequencing with enrichment of methylated DNA fragments and next generation sequencing. We produced least 23 million reads per sample, which were aligned to the goat reference genome. Further analyses were performed to identify peaks corresponding to hyper-methylated regions. We sequenced miRNAs expressed in the three tissues with Illumina high-throughput sequencing. Reads were mapped on the Capra hircus reference genome and both known and novel miRNAs, and miRNA target sites were identified using information collected in miRBase and using specific bioinformatic tools. This study produced a comprehensive miRNA profile related to the biology of goat. Furthermore, this is the first work dealing with methylome in Capra hircus: our preliminary results could provide new information for a deeper comprehension of epigenetic mechanisms of this species
The analysis of the methylome of Capra hircus
No full textMethylation at the carbon 5 position of cytosine residues is among the best studied epigenetic modification, and a fundamental layer of cellular differentiation through the control of transcriptional potential. DNA methylation patterns can be inherited and influenced by environment, diet and aging, and disrupted in diseases. Complete DNA methylomes for several organisms are now available, helping to clarify the evolutionary story of this epigenetic mark and its distribution in key genomic elements. At the present, methylome of the domestic goat, Capra hircus is unexplored, and hence to contribute information on epigenetic modification in the species, we analyzed the methylome of two tissues, ovary and hypothalamus, from 3 adult Saanen goats.
DNA methylation patterns are increasingly studied using high throughput sequencing methods. In order to evaluate differentially methylated region, we used Methylated DNA binding domain sequencing (MBD-seq), with enrichment of methylated DNA fragments and next generation sequencing (NGS - Hiseq 2000 Illumina).
We produced least 23 million reads per sample, which were aligned to the goat reference genome with a minimum success rate of 97.5%. Further analyses were performed to identify peaks corresponding to hyper-methylated regions. Matching the methylation pattern of hypothalamus versus ovaries of the three goats under study we found 4687 methylated fragments showing significant differences (P < 0.001) between tissues. Chromosomes 19 and 23 showed the highest density of methylated fragment, respectively every 0.56 Mb and 0.52 Mb in ovaries. Chromosomes 19 and 25 had the highest density of methylated fragment in the hypothalamus, every 0.58 Mb for both the chromosomes. The chromosome with least methylated fragments was the X, with a statistically significant (P < 0.001) methylated fragment every 9.38 Mb in the ovaries and 6.42 Mb in the hypothalamus. This is the first work dealing with methylome in Capra hircus: the outcoming pioneering preliminary results could be helpful for a deeper comprehension of the complex epigenetic machinery
DNA Methylation and Gene Expression Levels in Hypothalamus and Ovary of Capra hircus Across the Genome
No full textHypothalamus and ovary are two key organs of the female reproduction system of mammals. Their role in controlling ovulation is well known, but the epigenetic mechanisms behind it, such as DNA methylation, remain unclear. Goats are an important source of milk, meat and fiber, especially in developing countries. Despite this, among economically relevant livestock species, they are still poorly investigated from a genomic point of view. The aim of this work is to find a relation between expression levels and methylation levels, in order to understand how methylation peaks in different gene regions affect gene expression in goat hypothalamus and ovary. Genome-wide methylated CpG detection and gene expression analysis in hypothalamus and ovary of three adult Saanen goats were performed. Animals in the study were managed according to the existing European Directive 2010/63/EU on the protection of animals used for scientific purposes. For the methylome analysis DNA binding domain sequencing (MBD-seq) with enrichment of methylated DNA fragments was performed. Gene expression was evaluated by RNA-Seq analysis. Sequencing was accomplished with Hiseq 2000 Illumina. Around 23-37 million raw sequencing reads were generated from each sample for the methylome analysis. Methylation distribution was investigated in six different genomic regions: promoter, intron, exon, downstream of gene, distal and intergenic. Matching the methylation pattern in hypothalamus and ovaries in contrast to their transcriptome allowed the identification of genomic regions in which methylation peaks most affect gene expression. Hypothalamus showed a highly significant negative correlation (P<0.001) between methylation peaks in promoter and in downstream regions and gene expression. A positive correlation (P<0.001) was observed within exons. Conversely, ovary did not show any significant consistent correlation between gene expression and methylation. This work provides evidence for a clearer understanding of the epigenetic mechanisms underlying gene regulation. A more accurate annotation of the goat genome will be necessary for a deeper insight in the role of DNA methylation in gene expression in Capra hircus, a candidate model species for other mammals
La ricomposizione ambientale delle aree di cava a cielo aperto: il ruolo delle pedotecnologie
No full text“Paremiologia e tradizione sentenziosa nella Zucca del Doni in spagnolo”
No full textAlcune caratteristiche della traduzione spagnola della Zucca di Anton Francesco Doni (entrambe Venezia, Marcolini 1551) opportunamente evidenziate nel presente saggio dimostrano quali sono stati i due punti di riferimento principali del traduttore per quanto riguarda la trasposizione delle frasi proverbiali e di altri tipi di detti presenti nel testo originale: da un lato, la ricca tradizione popolare paremiologica che si ritrova nel refranero ma anche in un ampio ventaglio di testi che fanno del proverbio un’importante modalità espressiva, e dall’altro l’antico filone sapienziale, ancora vigente nella tradizione scritta. Proverbi e sentenze sono storicamente caratterizzati da tratti comuni ma, come si vedrà, pure da differenze, sebbene un buon numero di testi medievali non pare considerare rilevante l’aspetto differenziale; tanto i primi come le seconde sono presenti sia nella cultura dell’epoca sia nella traduzione qui studiata, ma in quest’ultima è precisa la linea che li separa, come si dimostra nel presente lavoro. Questa consapevolezza del traduttore è un fatto nuovo, che prelude alla visione moderna del fenomeno.The Zucca en Spañol, a XVIth century translation of Anton Francesco Doni’s Zucca (both Venezia, Marcolini, 1551), shows that the translator was familiar with folk proverbs and sayings of cultivated origins. The tradition of the 'refranero' (collections of proverbs and its popular counterpart, the 'refranes') and that of literary works that incorporated proverbs to the characters’ utterances were well established at that time; moreover, the medieval sententious literature was still very popular in Castile. Learned literature and popular tradition were often mixed in the same texts, while proverbs (together with refranes) and wise sayings were regarded as synonimous concepts. Our translator, on the contrary, proves himself conscious of the difference between these two types of quotes: an attitude that will became more and more widespread in the modern era
DNA methylation pattern of hypothalamus and ovary in Capra hircus
No full textOne of the key determinants in the control of gene expression in mammals, and the most commoncovalent modification of DNA in eukaryotes, is methylation at the carbon 5 of cytosine residues.DNA methylation patterns can be inherited and influenced by environment, diet and aging, and disrupted in diseases. Although methylomes from several tissues were investigated in some species, goats are still unexplored.We analysedthe methylome of hypothalamus and ovary from 3 adult Saanen goats, trying to take the first steps on the potential epigenetic involvement in goat biology.
In order to evaluate differentially methylated regions, we used Methylated DNA binding domain sequencing (MBD-seq), with enrichment of methylated DNA fragments and next generation sequencing (NGS- Hiseq2000 Illumina).We produced atleast 20 million reads per sample, covering an average of about 30% of the goat genome. Further analyses were performed to identify peaks corresponding to hyper-methylated regions. Chromosomes 12 and 20 showed the lowest density of methylated fragments on both tissues, while chromosomes 18 and 19 the highest.
We also investigated methylation distribution in the different genomic regions: promoter, intron, exon, downstream of gene, distal and intergenic. Introns showed the highest methylation frequency on both hypothalamus (34.6% on the total of the region detected) and ovary (39.1%).Matching the methylation pattern of hypothalamus versus ovaries of the three goats under study we looked for the biological and molecular pathways involving genes with tissue-specific methylation peaks. Pathways with the highest p-values (P < 0.001) affect RNA binding in ovary, and regulation of the immune system processes in hypothalamus.
This is the first work dealing with a global methylation pattern in Capra hircus: our pioneering results could be helpful for a deeper comprehension of the complex epigenetic machinery in this species
L’”indice pedoergonico” a supporto della programmazione insediativa territoriale e della progettazione urbana: un caso-studio nel bacino del Sarno in provincia di Napoli.
No full textP. R. Moore-Dewey's "Pregiudizio e Orgoglio": An Italian Re-Make of Austen's "Pride and Prejudicce"
No full textVirtually since its publication in 1814, Jane Austen's most famous novel, Pride and Prejudice, has enjoyed great success and has been the object of countless re-workings, re-mediations and adaptations on global scale. This essay aims at discussing a special instance of this phenomenon: an Italian re-make entitled Pregiudizio e Orgoglio (“Prejudice and Pride”), and written by Austen enthusiast and admirer, P.R. Moore-Dewey (pseudonym of Patrizia Murreddu). Appeared in Italy in 2012, to coincide with the bicentenary celebrations of its model and source, this title constitutes a genuine literary effort that deserves the attention of Austen enthusiasts, fans, and also scholars for its many merits, in particular that of being an intricate patchwork of quotations and, thus, a thoroughly post-modern work.
In this essay, I intend to examine Pregiudizio e Orgoglio's emblematic significance by outlining the numerous aspects that make it such a noteworthy adaptation of Austen. In particular, it is my intention to analyze the change of perspective adopted by the author, who decided to re-write Austen's tale through the point of view of Mr. Darcy and his sister Georgiana. Moreover, it is my intention to look at some of the many intertextual references present in Moore-Dewey's work (taken both from the whole Austen output, and from many other “classic” or “canonical” English writers) and consider the impact of such references and quotations on the reading experience. Finally, I will consider the extensive use of paratextual devices, which contribute to define this reworking a particularly relevant literary manifestation
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