368 research outputs found
A Bibliography of Ryuji Hattori, Japan at War and Peace:Shidehara Kijūrō and the Making of Modern Diplomacy(Canberra: Australian National University Press, 2021)
No full textapplication/pdfThe author recently published a book, Japan at War and Peace: Shidehara Kijūrō and the Making of
Modern Diplomacy (Canberra: Australian National University Press, 2021). It is open-access in multiple
formats at the URL https://press.anu.edu.au/publications/japan-war-and-peace. This book is based on
Ryuji Hattori, Zōhoban Shidehara Kijūrō: Gaikō to Minshushugi [Shidehara Kijūrō: Diplomacy and
Democracy, enlarged edition] (Tokyo: Yoshida Shoten, 2017). The English version was updated with a new
introduction and other information. However, due to the word limit, the bibliography was omitted.
Therefore, this paper provides that bibliography for the benefit of its readers.departmental bulletin pape
Involvement of the NADPH oxidase 2 pathway in renal oxidative stress in Aqp11-/- mice
No full textAquaporin-11 (AQP11) is an intracellular AQP. Several studies with Aqp11-/- mice have shown that AQP11 has a role in normal development of the kidney after birth. Our previous studies have suggested that alteration of oxygen homeostasis may be involved in the kidney injury caused by AQP11 deficiency, although the underlying mechanism is largely unknown. To clarify this issue, we examined genes that are related to oxygen homeostasis in Aqp11-/- mice. Among 62 genes that are involved in oxygen homeostasis, 35 were upregulated by more than 2-fold in Aqp11-/- mice in comparison with wild-type mice. Pathway analysis using these genes extracted the pathway responsible for production of reactive oxygen species in macrophages. As expression of the genes involved in the NADPH oxidase 2 (NOX2) complex was dramatically increased by more than 14-fold, we further analyzed NOX2 at the protein level. Immunoblotting analysis demonstrated a dramatic increase of NOX2 protein in the kidney of Aqp11-/- mice, and immunohistochemistry showed that NOX2 protein and a marker protein for macrophages were increased in the renal interstitium. These results indicate that NOX2-induced oxidative stress accompanied by macrophage infiltration plays an important role in alteration of oxygen homeostasis in Aqp11-/- mice.Yuya Hoshino, Hiroko Sonoda, Ryuji Nishimura, Kazuya Mori, Kenichi Ishibashi, Masahiro Ikeda,
Involvement of the NADPH oxidase 2 pathway in renal oxidative stress in Aqp11-/- mice,
Biochemistry and Biophysics Reports, Volume 17, 2019, Pages 169-176, ISSN 2405-5808,
https://doi.org/10.1016/j.bbrep.2019.01.003
Involvement of the NADPH oxidase 2 pathway in renal oxidative stress in Aqp11-/- mice
No full textAquaporin-11 (AQP11) is an intracellular AQP. Several studies with Aqp11-/- mice have shown that AQP11 has a role in normal development of the kidney after birth. Our previous studies have suggested that alteration of oxygen homeostasis may be involved in the kidney injury caused by AQP11 deficiency, although the underlying mechanism is largely unknown. To clarify this issue, we examined genes that are related to oxygen homeostasis in Aqp11-/- mice. Among 62 genes that are involved in oxygen homeostasis, 35 were upregulated by more than 2-fold in Aqp11-/- mice in comparison with wild-type mice. Pathway analysis using these genes extracted the pathway responsible for production of reactive oxygen species in macrophages. As expression of the genes involved in the NADPH oxidase 2 (NOX2) complex was dramatically increased by more than 14-fold, we further analyzed NOX2 at the protein level. Immunoblotting analysis demonstrated a dramatic increase of NOX2 protein in the kidney of Aqp11-/- mice, and immunohistochemistry showed that NOX2 protein and a marker protein for macrophages were increased in the renal interstitium. These results indicate that NOX2-induced oxidative stress accompanied by macrophage infiltration plays an important role in alteration of oxygen homeostasis in Aqp11-/- mice.Yuya Hoshino, Hiroko Sonoda, Ryuji Nishimura, Kazuya Mori, Kenichi Ishibashi, Masahiro Ikeda,
Involvement of the NADPH oxidase 2 pathway in renal oxidative stress in Aqp11-/- mice,
Biochemistry and Biophysics Reports, Volume 17, 2019, Pages 169-176, ISSN 2405-5808,
https://doi.org/10.1016/j.bbrep.2019.01.003
Interaction of homologues of Hsp70 and Cpn60 with ferredoxin-NADP+ reductase upon its import into chloroplasts
No full textAbstractA homologue of the 70-kDa heat-shock protein (Hsp70) was purified from pumpkin chloroplasts. The molecular mass of the purified protein was approximately 75 kDa and its N-terminal amino acid sequence was very similar to those of homologues of Hsp70 from bacterial cells and from the mitochondrial matrix and stroma of pea chloroplasts. The purified homologue of Hsp70 was found in the stroma of chloroplasts. To investigate the role(s) of the homologue of Hsp70 in the chloroplast stroma, we examined the possibility that the homologue of Hsp70 might interact with newly imported proteins to assist in their maturation (for example, in their folding and assembly). Ferredoxin NADP+ reductase (FNR) imported into chloroplasts in vitro could be immunoprecipitated with antisera raised against the homologue of Hsp70 from pumpkin chloroplasts and against GroEL from Escherichia coli, which is a bacterial homologue of chaperonin 60 (Cpn60), in an ATP-dependent manner, an indication that newly imported FNR interacts physically with homologues of Hsp70 and Cpn60 in chloroplasts. Time-course analysis of the import of FNR showed that imported FNR interacts transiently with the homologue of Hsp70 and that the association of FNR with the homologue of Hsp70 precedes that with the homologue of Cpn60. These results suggest that homologues of Hsp70 and Cpn60 in chloroplasts might sequentially assist in the maturation of newly imported FNR in an ATP-dependent manner
Techniques used in the Crystallization of the Earth: The Ryuji Kitagawa Mineral Collection exhibit
No full text富山市科学博物館では，平成27年7月18日から9月6日まで特別展「地球の結晶～北川隆司鉱物コレクション～」を開催した．本展では，北川隆司氏のコレクションを中心に，色や結晶の美しい鉱物標本約300点と㈲J．C．BAR所有の宝石標本約100点を展示した．本展では，鉱物の色や結晶の形の美しさを伝えるとともに，鉱物の様々な楽しみ方を提案することを狙いとし展示を構成した．展示の構成・デザインには，（１）来場者に展示の意図が伝わること，（２）当館の来館者層（幼児～小学生＋その保護者）にあった内容であること，（３）特に鉱物に興味をもたない来場者でも楽しめる展示であることに注意した．
本展開催期間中，来場者に本展の感想を問うアンケートを実施した．その結果，印象に残った展示として「宝石」を筆頭に，「光る石や色が変わる石」「鉱物の色」「鉱物の形」と回答した方が多く，自由記述においても，「きれいだった」といった感想が多く見られ，本展の狙いは概ね達成したと言える．For exhibits held at museums, curators plan and create exhibits matched to the nature of the exhibit venue and the visitors expected, carefully structuring the exhibit to suit the museum in question. The standard theory of exhibits has been discussed in various literature, but there have been few elucidations of the subtle exhibition techniques employed by curators. From July 18, 2015 through September 6, 2015, the Toyama Science Museum held an exhibit entitled: Crystallization of the Earth: The Ryuji Kitagawa Mineral Collection. This paper explains the exhibitional techniques that the author did in the exhibition. Further, the results of a survey distributed to visitors are used to assess future issues surrounding how exhibition techniques are evaluated by the public.departmental bulletin pape
Noninvasive Mechanochemical Imaging in Unconstrained Caenorhabditis elegans
No full textPhysical forces are transduced into chemical reactions, thereby ultimately making a large impact on the whole-animal level phenotypes such as homeostasis, development and behavior. To understand mechano-chemical transduction, mechanical input should be quantitatively delivered with controllable vibration properties–frequency, amplitude and duration, and its chemical output should be noninvasively quantified in an unconstrained animal. However, such an experimental system has not been established so far. Here, we develop a noninvasive and unconstrained mechanochemical imaging microscopy. This microscopy enables us to evoke nano-scale nonlocalized vibrations with controllable vibration properties using a piezoelectric acoustic transducer system and quantify calcium response of a freely moving C. elegans at a single cell resolution. Using this microscopy, we clearly detected the calcium response of a single interneuron during C. elegans escape response to nano-scale vibration. Thus, this microscopy will facilitate understanding of in vivo mechanochemical physiology in the future
In Situ Evaluation of the Polymer Concentration Distribution of Microphase-Separated Polyelectrolyte Hydrogels by the Microelectrode Technique
No full textThe heterogeneous structure that exists in virtually all hydrogels has a significant influence on the resulting strength and toughness. While the internal structure has been observed with electron microscopy, it is difficult to measure the in situ local polymer concentration in the native swollen state. In this study, a modified microelectrode technique (MET) was employed to measure the Donnan potential of a heterogeneous hydrogel with a phase-separated structure. With this method, we succeeded in observing quantitative in situ polymer concentrations ranging from 10.2 mu mol/L to several hundred mmol/L. From the obtained concentration profiles, we could successfully evaluate the internal phase-separated structure with a resolution of less than 0.8 mu m. Using MET, we could estimate the average activity coefficient of the hydrogel, and we found a difference in concentration between the dense and sparse phases. We demonstrate that MET is a powerful method that can locally and quantitatively measure the polyelectrolyte concentration distribution within hydrogels. Furthermore, this method can be applied to cells and organs in vivo due to their similarities with polyelectrolytes. Enabling the in situ determination of the internal structures of biomaterials could have important implications toward the characterization of damaged and diseased tissues on the local scale
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