15 research outputs found
Development of a disposable electrochemical sensor for detection of cholesterol using differential pulse voltammetry
No full textIn this study, a sensitive and selective electrochemical sensor was fabricated by usinga screen printed carbon electrode (SPCE), multi-walled carbon nanotubes (MWCNTs) and beta-cyclodextrin (beta-CD) for detecting cholesterol. MWCNTs were functionalized with benzoic acid moiety by employing diazonium salt chemistry, and, subsequently, a thin film of functionalized CNTs were coated on the surface of SPCE. Afterwards, beta-CD was immobilized on functionalized MWCNTs modified SPCE which acts as a host to recognize guest (cholesterol) molecule specifically. Under the optimal experimental conditions and using differential pulse voltammetry (DPV) as transduction technique the sensor was able to detect cholesterol level ranges from 1 nM to 3 mu M, with a detection limit of 0.5 nM. Specificity of the developed sensor towards target analyte (cholesterol) was confirmed in the presence of common interfering species including glucose, uric acid and ascorbic acid. The applicability of proposed sensor was also demonstrated for cholesterol determination in human serum samples with good recovery results (94-96%) and maximum RSD (relative standard deviation) of 4.5%. (C) 2018 Elsevier B.V. All rights reserved.HEC (Higher Education Commission of Pakistan)-NRPU project [20-4286/RD/HEC/2014]This work was carried out under the HEC (Higher Education Commission of Pakistan)-NRPU project; No. 20-4286/R&D/HEC/2014 of Akhtar Hayat
Nano-Engineered Biomimetic Optical Sensors for Glucose Monitoring in Diabetes
No full textDiabetes is a rapidly growing disease that can be monitored at an individual level by controlling the blood glucose level, hence minimizing the negative impact of the disease. Significant research efforts have been focused on the design of novel and improved technologies to overcome the limitations of existing glucose analysis methods. In this context, nanotechnology has enabled the diagnosis at the single cell and molecular level with the possibility of incorporation in advanced molecular diagnostic biochips. Recent years have witnessed the exploration and synthesis of various types of nanomaterials with enzyme-like properties, with their subsequent integration into the design of biomimetic optical sensors for glucose monitoring. This review paper will provide insights on the type, nature and synthesis of different biomimetic nanomaterials. Moreover, recent developments in the integration of these nanomaterials for optical glucose biosensing will be highlighted, with a final discussion on the challenges that must be addressed for successful implementation of these nano-devices in the clinical applications is presented
Multifunctional Smart ZnSe-Nanostructure-Based Fluorescent Aptasensor for the Detection of Ochratoxin A
No full textHerein, we present a comprehensive investigation of rationally designed zinc selenide (ZnSe) nanostructures to achieve highly negatively charged ZnSe nanostructures. A Microwave-assisted hydrothermal synthesis method was used to synthesize three types of ZnSe nanostructures, i.e., nanorods, µ-spheres and nanoclusters, as characterized by a zeta potential analyzer, X-ray diffraction (XRD), scanning electron microscopy (SEM), Raman spectroscopy and BET, which were labeled as type A, B and C. Three different solvents were used for the synthesis of type A, B and C ZnSe nanostructures, keeping other synthesis conditions such as temperature, pressure and precursors ratio constant. Based on two heating time intervals, 6 and 9 h, types A, B and C were further divided into types A6, A9, B6, B9, C6 and C9. ZnSe nanostructures were further evaluated based on their fluorescent quenching efficiency. The maximum fluorescence quenching effect was exhibited by the ZnSe-B6 type, which can be attributed to its highly negative surface charge that favored its strong interaction with cationic dye Rhodamine B (Rh-B). Further, the optimized ZnSe-B6 was used to fabricate an aptasensor for the detection of a food-based toxin, ochratoxin-A (OTA). The developed aptasensor exhibited a limit of detection of 0.07 ng/L with a wide linear range of 0.1 to 200 ng/L
Electrospinning of graphene-oxide onto screen printed electrodes for heavy metal biosensor
No full textInternational audienc
One Step Assembly of Thin Films of Carbon Nanotubes on Screen Printed Interface for Electrochemical Aptasensing of Breast Cancer Biomarker
No full textThin films of organic moiety functionalized carbon nanotubes (CNTs) from a very well-dispersed aqueous solution were designed on a screen printed transducer surface through a single step directed assembly methodology. Very high density of CNTs was obtained on the screen printed electrode surface, with the formation of a thin and uniform layer on transducer substrate. Functionalized CNTs were characterized by X-ray diffraction spectroscopy (XRD), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and Brunauer–Emmett– Teller (BET) surface area analyzer methodologies, while CNT coated screen printed transducer platform was analyzed by scanning electron microscopy (SEM), atomic force microscopy (AFM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The proposed methodology makes use of a minimum amount of CNTs and toxic solvents, and is successfully demonstrated to form thin films over macroscopic areas of screen printed carbon transducer surface. The CNT coated screen printed transducer surface was integrated in the fabrication of electrochemical aptasensors for breast cancer biomarker analysis. This CNT coated platform can be applied to immobilize enzymes, antibodies and DNA in the construction of biosensor for a broad spectrum of applications
Protic ionic liquids as a versatile modulator and stabilizer in regulating artificial peroxidase activity of carbon materials for glucose colorimetric sensing
No full textInternational audienc
THE CONCEPT OF MORAL EDUCATION IN THE QUR'AN IN SDIT DARUL HUFFADZ BEKASI REGENCY
No full textThis study aimed to explain the concept of al-Qur'an moral education at SDIT Darul Huffadz Bekasi. The research method used by the author is a type of qualitative research through a field research approach, by collecting data or materials related to the theme of the discussion and the problem, taken from trusted sources through structured interviews as well as observation and documentation, then analyzed coherently, describing all aspects involved and contained in the verses of the Qur'an which are interpreted and describe the meaning contained in the Qur'an. To support this research, the author uses supporting sources from other literature such as books, scientific journals, and so on related to the discussion. The study results stated that SDIT Darul Huffadz Bekasi has a character-building program through Islamic Religious Education with the methods used in its implementation, namely the habit method, the exemplary method and the punishment method.ABSTRACT: The purpose of this study was: to determine the concept of moral education in the Qur'an at SDIT Darul Huffadz Bekasi Regency. The research method used by the author is a type of qualitative research through library research, by collecting data or materials related to the theme of the discussion and the problem, which is taken from library sources, then analyzed by the taḫlili method, namely the method of interpreting the verses of the Qur'an which is carried out by describing all aspects contained in the interpreted verses of the Qur'an and describing the descriptions of the meanings contained therein. To support this research, the author uses the main sources of interpretation books, including Tafsir Jami' al-Bayan an Ta'wil Ayi al-Qur'an (Tafsir ath-Thabari) by Abu Ja'far Muhammad bin Jarir Ath-Thabari, Tafsir al-Tabari, Azhar by Abdul malik Abdulkarim Amrullah (Hamka), and Tafsir al-Misbah by M. Quraish Shihab.Based on the results of this study, the authors found that in Surah al-An'ām Verses 151-153 contained the values of moral education, including: the value of monotheism, birrul wālidain, protection of offspring, maintaining self-respect, protection of the soul, maintaining (loving ) orphans, honest and fair in commerce and in testimony, keeping promises, and being obedient and obedient to regulations. Some of these educational values can be implemented in schools and in everyday life by using several methods including the mau'izhah method (advice), the uswatun asanah (exemplary) method, the ta'wîd method (habituation), and the tarġîb and tarhîb methods
RutheniumII(η6-arene) Complexes of Thiourea Derivatives: Synthesis, Characterization and Urease Inhibition
No full textRuII(arene) complexes have emerged as a versatile class of compounds to design metallodrugs as potential treatment for a wide range of diseases including cancer and malaria. They feature modes of action that involve classic DNA binding like platinum anticancer drugs, may covalent binding to proteins, or multimodal biological activity. Herein, we report the synthesis and urease inhibition activity of RuII(arene) complexes of the general formula [RuII(η6-p-cymene)(L)Cl2] and [RuII(η6-p-cymene)(PPh3)(L)Cl]PF6 with S-donor systems (L) based on heterocyclic thiourea derivatives. The compounds were characterized by 1H-, 13C{1H}- and 31P{1H}-NMR spectroscopy, as well as elemental analysis. The crystal structure of [chlorido(η6-p-cymene)(imidazolidine-2-thione)(triphenylphosphine)ruthenium(II)] hexafluorophosphate 11 was determined by X-ray diffraction analysis. A signal in the range 175–183 ppm in the 13C{1H}-NMR spectrum indicates the presence of a thione rather than a thiolate. This observation was also confirmed in the solid state by X-ray diffraction analysis of 11 which shows a C=S bond length of 1.720 Å. The compounds were tested for urease inhibitory activity and the thiourea-derived ligands exhibited moderate activity, whereas their corresponding Ru(arene) complexes were not active
