346 research outputs found
Letter from The Dominguez Estate Company to Mr. K.F. Nance, April 1, 1942
No full textAgreeing to the proposal that Mr. Nance take over the Shimono lease
Tyr-199 and charged residues of pharaonis Phoborhodopsin are important for the interaction with its transducer.
No full textpharaonis Phoborhodopsin (ppR; also pharaonis sensory rhodopsin II, psRII) is a retinal protein in Natronobacterium pharaonis and is a receptor of negative phototaxis. It forms a complex with its transducer, pHtrII, in membranes and transmits light signals by protein-protein interaction. Tyr-199 is conserved completely in phoborhodopsins among a variety of archaea, but it is replaced by Val (for bacteriorhodopsin) and Phe (for sensory rhodopsin I). Previously, we (Sudo, Y., M. Iwamoto, K. Shimono, and N. Kamo, submitted for publication) showed that analysis of flash-photolysis data of a complex between D75N and the truncated pHtrII (t-Htr) give a good estimate of the dissociation constant KD in the dark. To investigate the importance of Tyr-199, KD of double mutants of D75N/Y199F or D75N/Y199V with t-Htr was estimated by flash-photolysis and was ~10-fold larger than that of D75N, showing the significant contribution of Tyr-199 to binding. The KD of the D75N/t-Htr complex increased with decreasing pH, and the data fitted well with the Henderson-Hasselbach equation with a single pKa of 3.86 ± 0.02. This suggests that certain deprotonated carboxyls at the surface of the transducer (possibly Asp-102, Asp-104, and Asp-106) are needed for the binding
Condições de venda e adaptação de lentes de contato nos estabelecimentos óticos do município de Florianópolis
No full textTrabalho de Conclusão de Curso - Universidade Federal de Santa Catarina. Curso de Medicina. Dapartamento de Clínica Cirúrgica
Figure 8 in Developmental stages and growth of Pseudocaligus fugu Yamaguti, 1936 (Copepoda: Siphonostomatoida: Caligidae) host-specific to Puffer
No full textFigure 8. Pseudocaligus fugu, fourth chalimus stage. Female (A–N), male (O–R). (A, O) Habitus, dorsal view; (B) frontal filament; (C) left caudal ramus, ventral view; (D) antennule, antenna and postantennary process in situ; (E) distal segment of antennule; (F) maxillule; (G) maxilla; (H) maxilliped; (I) right leg 1, anterior surface; (J) endopod of right leg 1, anterior surface; (K) leg 2, anterior surface; (L) leg 3, anterior surface; (M, Q) left leg 4; (N, R) leg 5; (P) antenna and postantennary process. Scales in mm.Published as part of Ohtsuka, Susumu, Takami, Ikuo, Maran, B.A. Venmathi, Ogawa, Kazuo, Shimono, Takaki, Fujita, Yusuke, Asakawa, Manabu & Boxshall, Geoffrey A., 2009, Developmental stages and growth of Pseudocaligus fugu Yamaguti, 1936 (Copepoda: Siphonostomatoida: Caligidae) host-specific to Puffer, pp. 1779-1804 in Journal of Natural History 43 (29-30) on page 1791, DOI: 10.1080/00222930902993757, http://zenodo.org/record/521666
Characteristics of splenic CD8+ T cell exhaustion in patients with hepatitis C
No full textSummary: There is increasing interest in the role of T cell exhaustion and it is well known that the natural history of chronic hepatitis C virus infection (HCV) is modulated by CD8+ T cell immunobiology. There are many pathways that alter the presence of exhaustive T cells and, in particular, they are functionally impaired by inhibitory receptors, such as programmed death-1 (PD-1) and T cell immunoglobulin and mucin domain-containing protein 3 (Tim-3). We obtained spleen, liver and peripheral blood (before and after splenectomy) lymphoid cells from 25 patients with HCV-related cirrhosis undergoing liver transplantation for end-stage disease or splenectomy for portal hypertension. In all samples we performed an extensive phenotypic study of exhaustion markers [PD-1, Tim-3, interferon (IFN)-γ) and their ligands (PD-L1, PD-L2, galectin-9] in CD8+ T cell subpopulations (both total and HCV-specific) and in antigen-presenting cells (APC; monocytes and dendritic cells). In the spleen, total and HCV-specific CD8+ T cells demonstrated enhanced markers of exhaustion, predominantly in the effector memory subpopulation. Similarly, splenic APC over-expressed inhibitory receptor ligands when compared to peripheral blood. Finally, when peripheral blood CD8+ T cells were compared before and after splenectomy, markers of exhaustion were reduced in splenic CD8+ T cells and APC. Our data in HCV-related cirrhosis suggest that CD8+ T cells in the spleen manifest a significantly higher exhaustion compared to peripheral blood and may thus contribute to the failure to control HCV. Counteracting this process may contribute to inducing an effective immune response to HCV
Association between a photo-intermediate of a M-lacking mutant D75N of pharaonis phoborhodopsin and its cognate transducer.
No full textPharaonis phoborhodopsin (ppR or pharaonis sensory rhodopsin II) is a receptor of the negative phototaxis of Natronobacterium pharaonis and forms a complex with its transducer pHtrII in membranes. Flash-photolyis of a D75N mutant did not yield the M-intermediate, but an O-like intermediate is observed in a ms time range. We examined the interaction between the D75N of ppR and t-Htr (truncated pHtrII). These formed a complex in the presence of 0.1% n-dodecyl-β-maltoside, and the association accelerated the decay of the O of D75N from 15 to 56 s−1. From the decay time constants under varying ratios of D75N and t-Htr, n, the molar ratio of D75N/t-Htr in the complex, and KD, the dissociation constant, were estimated. The value of n was unity and KD was estimated to 146 nM. This KD value can be considered to be the association between the photo-intermediate and t-Htr, which is deduced by the method of estimation. Previously we (Photochem. Photobiol. 74 (2001) 489) reported a KD of 15 μM for the interaction between the wild-type and t-Htr by means of the change in M-decay rates. Therefore, this value should be the KD value for the interaction between M of the wild-type and t-Htr
Letter from The Dominguez Estate Company, to Mr. M. Shimono , February 19, 1942
No full textRegarding attached acreage lease
N Uptake Ability under Interrupted N Supply in Day⁄Night in Response to Transpiration Demand in rice
No full textjournal articl
Author Correction: Efficient communication dynamics on macro-connectome, and the propagation speed
No full textA correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.</jats:p
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