1,396,729 research outputs found
Insights into the Musa genome: Syntenic relationships to rice and between Musa species
No full textAbstract Background Musa species (Zingiberaceae, Zingiberales) including bananas and plantains are collectively the fourth most important crop in developing countries. Knowledge concerning Musa genome structure and the origin of distinct cultivars has greatly increased over the last few years. Until now, however, no large-scale analyses of Musa genomic sequence have been conducted. This study compares genomic sequence in two Musa species with orthologous regions in the rice genome. Results We produced 1.4 Mb of Musa sequence from 13 BAC clones, annotated and analyzed them along with 4 previously sequenced BACs. The 443 predicted genes revealed that Zingiberales genes share GC content and distribution characteristics with eudicot and Poaceae genomes. Comparison with rice revealed microsynteny regions that have persisted since the divergence of the Commelinid orders Poales and Zingiberales at least 117 Mya. The previously hypothesized large-scale duplication event in the common ancestor of major cereal lineages within the Poaceae was verified. The divergence time distributions for Musa-Zingiber (Zingiberaceae, Zingiberales) orthologs and paralogs provide strong evidence for a large-scale duplication event in the Musa lineage after its divergence from the Zingiberaceae approximately 61 Mya. Comparisons of genomic regions from M. acuminata and M. balbisiana revealed highly conserved genome structure, and indicated that these genomes diverged circa 4.6 Mya. Conclusion These results point to the utility of comparative analyses between distantly-related monocot species such as rice and Musa for improving our understanding of monocot genome evolution. Sequencing the genome of M. acuminata would provide a strong foundation for comparative genomics in the monocots. In addition a genome sequence would aid genomic and genetic analyses of cultivated Musa polyploid genotypes in research aimed at localizing and cloning genes controlling important agronomic traits for breeding purposes.</p
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Musa Barkeji on Closing the Breast Cancer Survival Gap for Black Women
No full textIn this vital episode of Voices of Resilience, host Mary Mosoeunyane speaks with Mr. Musa Barkeji, Consultant General and Oncoplastic Surgeon at Chelsea and Westminster NHS Foundation Trust and Honorary Senior Lecturer at Imperial College London. With over two decades of experience in breast cancer surgery, Mr. Barkeji sheds light on why cancer outcomes for Black women remain disproportionately poor — and what must change to close the survival gap.
Drawing on both research and frontline clinical practice, Musa explains the complex web of factors that contribute to these disparities: from late-stage diagnoses and limited awareness to cultural taboos, mistrust of the healthcare system, and underrepresentation in clinical trials. He speaks candidly about the myths and fears that still surround cancer in Black communities — including the silence that often delays lifesaving treatment — and urges a collective effort to normalise open conversations about breast health.
Mary and Musa also explore the transformative role of oncoplastic surgery — a field that combines cancer treatment with reconstructive techniques to preserve body image and confidence. He offers powerful insights into how culturally sensitive outreach, community engagement through churches and grassroots groups, and earlier screening could dramatically improve outcomes for Black women.
This episode is both educational and urgent — a call to action for awareness, early detection, and compassion in cancer care. As Musa reminds us, “Breast cancer is real — but survival depends on how soon we act.
Iktibar taubat Nabi Musa As
No full textRencana membincangkan hikmah taubat nabi Musa sebagaimana yang disebut al-Quran melalui surah al-Qasa
Major achievements of the EC MUSA project
No full textInternational audienceMaturity of severe accident (SA) codes, progress in the computational methods, and computer infrastructures were considered a sound platform to conduct, for the first time in SA modelling, a systematic and broad application of Uncertainty and Sensitivity Analysis (UaSA) in this domain. The overall objective of the HORIZON-2020 project on "Management and Uncertainties of Severe Accidents (MUSA)" was to quantify the uncertainties of SA integral codes when modelling reactor and spent fuel pool (SFP) accident scenarios of Gen II and Gen III reactor designs for the prediction of the radiological source term. This paper presents the major achievements accomplished by MUSA. To name a few: a database on input parameter uncertainties has been assembled; SA codes and statistical tools, either in-house built or existing ones, have been properly coupled by scripting or interfaces; major specific challenges have been identified and different solutions have been worked out; and, applications to several reactor types and SFP scenarios have shown interesting insights of this simulation approach, particularly when dealing with Source Term variables as figures of merit. No less important, major challenges were found and are here discussed as needs to be addressed before achieving an effective implementation of UaSA in the SA domain
Analysis of non-TIR NBS-LRR resistance gene analogs in <it>Musa acuminata </it>Colla: Isolation, RFLP marker development, and physical mapping
No full textAbstract Background Many commercial banana varieties lack sources of resistance to pests and diseases, as a consequence of sterility and narrow genetic background. Fertile wild relatives, by contrast, possess greater variability and represent potential sources of disease resistance genes (R-genes). The largest known family of plant R-genes encode proteins with nucleotide-binding site (NBS) and C-terminal leucine-rich repeat (LRR) domains. Conserved motifs in such genes in diverse plant species offer a means for isolation of candidate genes in banana which may be involved in plant defence. Results A computational strategy was developed for unbiased conserved motif discovery in NBS and LRR domains in R-genes and homologues in monocotyledonous plant species. Degenerate PCR primers targeting conserved motifs were tested on the wild cultivar Musa acuminata subsp. burmannicoides, var. Calcutta 4, which is resistant to a number of fungal pathogens and nematodes. One hundred and seventy four resistance gene analogs (RGAs) were amplified and assembled into 52 contiguous sequences. Motifs present were typical of the non-TIR NBS-LRR RGA subfamily. A phylogenetic analysis of deduced amino-acid sequences for 33 RGAs with contiguous open reading frames (ORFs), together with RGAs from Arabidopsis thaliana and Oryza sativa, grouped most Musa RGAs within monocotyledon-specific clades. RFLP-RGA markers were developed, with 12 displaying distinct polymorphisms in parentals and F1 progeny of a diploid M. acuminata mapping population. Eighty eight BAC clones were identified in M. acuminata Calcutta 4, M. acuminata Grande Naine, and M. balbisiana Pisang Klutuk Wulung BAC libraries when hybridized to two RGA probes. Multiple copy RGAs were common within BAC clones, potentially representing variation reservoirs for evolution of new R-gene specificities. Conclusion This is the first large scale analysis of NBS-LRR RGAs in M. acuminata Calcutta 4. Contig sequences were deposited in GenBank and assigned numbers ER935972 – ER936023. RGA sequences and isolated BACs are a valuable resource for R-gene discovery, and in future applications will provide insight into the organization and evolution of NBS-LRR R-genes in the Musa A and B genome. The developed RFLP-RGA markers are applicable for genetic map development and marker assisted selection for defined traits such as pest and disease resistance.</p
Agrobacterium tumefaciens transformation of Musa species
No full textMethods are provided for transforming Musa plants. In particular, methods for wounding meristematic Musa plant tissue to facilitate access of Agrobacterium tumefaciens comprising genetically-engineered T-DNA is provided. The methods may be used to transform the plant to produce pharmaceutical products or to alter the phenotypic trait of the fruit of the plant.U
Musa Jarullah Bigiyev
No full textMusa Jārullāh Bigiyev was a Muslim Tatar religious scholar, journalist, politician, educator and a prolific writer, who devoted his entire life to reconciling Islam with modern progress. He published more than sixty books in Arabic and Old Ottoman dealing with the issues of Islamic jurisprudence, theology, sciences of the Qur’an, sciences of the hadith, literature, economics, law, politics and history
Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp)
No full textABSTRACT. We searched the genome of Mycosphaerella fijiensis for molecular markers that would allow population genetics analysis of this plant pathogen. M. fijiensis, the causal agent of banana leaf streak disease, also known as black Sigatoka, is the most devastating pathogen attacking bananas (Musa spp). Recently, the entire genome sequence of M. fijiensis became available. We screened this database for VNTR markers. Forty-two primer pairs were selected for validation, based on repeat type and length and the number of repeat units. Five VNTR markers showing multiple alleles were validated with a reference set of isolates from different parts of the world and a population from a banana plantation in Costa Rica. Polymorphism information content values varied from 0.6414 to 0.7544 for the reference set and from 0.0400 and 0.7373 for the population set. Eighty percent of the polymorphism information content values were above 0.60, indicating that the markers are highly informative. These markers allowed robust scoring of agarose gels and proved to be useful for variability and population genetics studies. In conclusion, the strategy we developed to identify and validate VNTR markers is an efficient means to incorporate markers that can be used for fungicide resistance management and to develop breeding strategies to control banana black leaf streak disease. This is the first report of VNTR-minisatellites from the M. fijiensis genome sequence. Key words: Molecular markers; VNTRs; Genetic diversity; Population genetics; Black Sigatok
The Diary of Musa Mai Almajirai
No full textDate created: unknown. The entire manuscript is available for download below as a single PDF file. Because of the large size of this manuscript, it is also available in three partial PDF files. In addition, each page is available as a separate, larger, JPG file. If higher-resolution JP2 files are needed (WARNING: files average 11-14MB in size), please contact [email protected]. Fieldwork Team: Mustapha Kurfi (PI, Hausa Ajami Scholar), Abdurra'uf Hashim (Research Assistant) and Bara'u Musa (Research Assistant). Technical Team: Vika Zafrin (Institutional Repository Librarian, Boston University Libraries), Dr. Fallou Ngom (Director, African Language Program), Dr. Peter Quella (Assistant Director, African Studies Center), and Zachary Gersten (Coordinator, African Language Program). This collection of Hausa Ajami materials is copied as part of the African Studies Center's African Ajami Library. This project is funded by the Boston University African Studies Center. We thank Prof. Tim Longman, Director of the African Studies Center, and the entire African Studies Team for their support. Access Condition and Copyright: The materials are subject to copyright. Access is for research and educational purposes only. Materials are not to be reproduced without written permission. Citation: Kurfi, Mustapha and Ngom, Fallou. 2015. African Ajami Library: Digital Preservation of Hausa Ajami Manuscripts of Nigeria. Boston: Boston University Library: http://hdl.handle.net/2144/11726 For Inquires: Please, contact Professor Fallou Ngom ([email protected]).The material, entitled Kundin Malam Musa Mai Almajirai (The Diary of Malam Musa Mai Almajirai), is a collection of Hausa Ajami Islamic medicine manuscripts that belongs to the family of Malam Musa Mai Almajirai (born in 1932). Many of the materials are khatims (magical squares) that serve to identify client's ailments and appropriate treatments. These cover many aspects of the human endeavor, ranging from love and relationships, winning court cases, healing unidentified ailments, treatments for body pains and aches, and recipes for braveness and popularity. Certain materials in this collection are hard to comprehend, except by the authors and trained practitioners, as many of the recipes are deliberately not included. This omission is similar to modern day password, thus, a built-in security measure that denies unauthorized access. The collection contains 201 pages in total
Toli Masjid
No full textLocated on the road leading to Golconda fort from the Purana Pul. It was built by Musa Khan, mahaldar, chamberlain of Abdullah Qutb Shah in 1671. Built on a 6 foot elevated terrace, with a flight of steps on north, south and east. The mosque is divided into two halls, the outer hall has five arched openings while the inner hall has three. Two sixty foot minarets flank the two sides of the mosque. Musa Khan, who superintended construction of the Mecca Mosque was given a commission of one Toli or Damri, equaling one fourth of a pice per rupee when he constructed the Mecca Mosque. The mosque is so named after the commission the builder received for Mecca Mosque construction.exterior, exterior view from east, 192
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