1,568 research outputs found
Dimer-dimer stacking interactions are important for nucleic acid binding by the archaeal chromatin protein Alba
No full textArchaea use a variety of small basic proteins to package their DNA. One of the most widespread and highly conserved is the Alba (Sso10b) protein. Alba interacts with both DNA and RNA in vitro, and we show in the present study that it binds more tightly to dsDNA (double-stranded DNA) than to either ssDNA (single-stranded DNA) or RNA. The Alba protein is dimeric in solution, and forms distinct ordered complexes with DNA that have been visualized by electron microscopy studies; these studies suggest that, on binding dsDNA, the protein forms extended helical protein fibres. An end-to-end association of consecutive Alba dimers is suggested by the presence of a dimer-dimer interface in crystal structures of Alba from several species, and by the strong conservation of the interface residues, centred on Are and Phe(60). In the present study we map perturbation of the polypeptide backbone of Alba upon binding to DNA and RNA by NMR, and demonstrate the central role of Phe(60) in forming the dimer dimer interface. Site-directed spin labelling and pulsed ESR are used to confirm that an end-to-end, dimer dimer interaction forms in the presence of dsDNA.Peer reviewe
First person – Alba Delrio-Lorenzo
No full textFirst Person is a series of interviews with the first authors of a selection of papers published in Journal of Cell Science, helping early-career researchers promote themselves alongside their papers. Alba Delrio-Lorenzo is first author on ‘Sarcoplasmic reticulum Ca2+ decreases with age and correlates with the decline in muscle function in Drosophila’, published in JCS. Alba is a PhD student in the lab of Javier García-Sancho and María Teresa Alonso at the Instituto de Biología y Genética Molecular (IBGM), University of Valladolid, Spain, investigating the molecular mechanisms implicated in aging, particularly muscle aging.Peer reviewe
Marianella Morena and Lola Arias: memory and testimony from the poetics of the real
No full textEste artículo construye un diálogo entre la poética de las teatristas Marianella Morena
(Uruguay) y Lola Arias (Argentina), quienes desarrollan su labor en un contexto geográfico hermanado, la escena rioplatense. Las propuestas seleccionadas aúnan dos elementos
comunes: el tratamiento escénico de la memoria sobre la violencia dictatorial y su trabajo
desde las prácticas de lo real. Según postulamos, el análisis de Antígona Oriental de Morena
y Campo minado de Arias posibilita reflexionar sobre las vías de trabajo en la construcción
memorial desde la convocatoria del testimonio y la afectividad como mecanismo artístico
y relacional con el público.This article constructs a dialogue between the poetics of the theatrical artists Marianella
Morena (Uruguay) and Lola Arias (Argentina), who develop their work in a twinned geographical context, the Rio de la Plata scene. The selected proposals combine two common
elements: the scenic treatment of the memory of the dictatorial violence and their work
from the practices “of the real”. As we postulate, the analysis of Antígona Oriental by Morena
and Campo Minado by Arias makes it possible to reflect on the ways of working in the construction of memory from the call for testimony and affectivity as an artistic and relational
mechanism with the audience
Conocer y valorar el patrimonio cultural de Sierra Morena : un proyecto del IAPH y ADIT-Sierra Morena
No full textDurante 2008 el Instituto Andaluz de Patrimonio Histórico formalizó la colaboración con la Asociación para el Desarrollo de Sierra Morena Cordobesa y con la Asociación para el Desarrollo Integral del Territorio de Sierra Morena (ADITMorena) con el objetivo de fomentar e incentivar la gestión sostenible del patrimonio cultural en el territorio con la participación de los agentes sociales. El artículo presenta los criterios teóricos y metodológicos de partida junto a todas las acciones realizadas en las distintas fases del proyecto: localización y consulta de fuentes, desarrollo del trabajo de campo y diseño de dos itinerarios turístico-culturales por Sierra Morena. Se finaliza con el deseo de continuar con este tipo de iniciativas que apuestan por el conocimiento y la preservación del legado patrimonial y por convertir los bienes patrimoniales en recursos que repercutan positivamente en la calidad de vida de la población y en la mejora de los proyectos de desarrollo
local directamente relacionados con el patrimonio cultural
The Author/Translator Interactional Process. A Case Study
No full textSee Naples and Kill (1988) is a lively and colourful novel by the con-temporary English writer, Gregory Dowling, translated into Italian in 2015.
Following the tradition of translation studies (Venuti 2000, Bass-nett 2002, Cronin 2006), this paper analyses the rewriting process of literary translation, considering in particular the fruitful but sometimes tense and even conflictual relationship between writer and translator.
The translation of the novel See Naples and Kill was an ongoing rewriting process entailing a constant dialogue between the writer and the translator. Therefore, the study aims at answering two main ques-tions: what happens if the rewriting process of translation is constant-ly questioned by the author? What happens if the author has a good mastery of the target language and s/he is her/himself a translator?
By exploring the relationship between translation and re-creation, the research focuses on the differences and similarities between the primary creation (source text) and the secondary creation (target text), and aims to verify in which way the dialogic encounter of two different personalities and cultures does not make them merge but, by retaining their own uniqueness, leads eventually to their mutually en-riching each other. A comparative analysis of the source text and the different drafts of the translated version accompanied by the author’s comments will shed light on the tense author-translator relationship in the specific case under investigation and how both actors handle this tension in order to create a new work resulting from the (dis)agreement of the two parties
Lope después de Lope : su difusión y fortuna, de la «Fama póstuma» a nuestros días.
No full textCada época, cada momento histórico, ha tenido a un Lope de Vega. No es que la obra del Fénix cambiase tras su muerte, sino que las distintas generaciones de escritores y lectores se han acercado a su producción sin poder abstraerse de las preocupaciones ideológicas y estéticas propias de su tiempo.
En este libro, formado por trece trabajos de reconocidos investigadores de distintas generaciones, aspiramos a trazar algunas de las líneas principales que fueron construyendo una imagen poliédrica del escritor, en más de una ocasión velada por juicios propios de la época. El itinerario propuesto abarca desde las más tempranas imitaciones poéticas hasta la eclosión artística que supuso el redescubrimiento de Lope de Vega 1935 con motivo del Tricentenario (puestas en escena, recreaciones musicales, difusión en artículos de prensa...). Tampoco se olvidan por el camino la escurridiza recepción sobre las tablas y entre los estudiosos que se dio en los siglos XVIII y XIX, ni menos aún la actual puesta en escena. Con la visión de conjunto sobre el tema que aquí proponemos, se puede apreciar mucho mejor la construcción de la imagen de Lope de Vega como un mito, a la vez escritor laureado e ilustre desconocido para los ilustrados, dramaturgo de referencia y nuevo objeto de estudio para los eruditos y los hombres y mujeres de teatro del siglo XIX, icono cultural y constante fuente de inspiración para la sociedad contemporánea y actual.UK Research and InnovationDepto. de Literaturas Hispánicas y BibliografíaFac. de FilologíaTRUEpu
A ALBA e a luta pela hegemonia no continente americano: uma topologia do discurso bolivariano
No full textDissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Filosofia e Ciências Humanas. Programa de Pós-graduação em Sociologia PolíticaO objetivo da dissertação é analisar o movimento bolivariano, liderado pela Venezuela, enquanto projeto de construção de uma nova hegemonia na América Latina e o Caribe. Tal projeto se constrói discursivamente em oposição à histórica hegemonia pan-americana liderada pelos Estados Unidos (EUA). Para isso é estudada a ALBA (Aliança Bolivariana para os Povos de nossa América) como organização internacional que facilita a alineação de países e sociedades latino-americanos em relação ao movimento bolivariano, gerando novas modalidades de construção de sentido, assim como novas vias e regras de relacionamento internacional. A pesquisa analisa os discursos de mandatários nas reuniões de Cúpula da ALBA, e, apelando à teoria do discurso de Ernesto Laclau, identifica nesse conjunto os três passos previstos pelo autor na configuração de um desafio hegemônico: (1) o estabelecimento de um antagonismo face ao imperialismo dos EUA que é identificado como o poder hegemônico institucionalizado, e a dicotomização do espaço social americano através da construção de uma fronteira entre esse poder e o resto da comunidade (América Latina e o Caribe); (2) a articulação contingente entre diversas demandas sociais, características da América Latina e do Caribe, cuja insatisfação é atribuída ao imperialismo dos EUA; e (3) a formação de um campo popular, através da expressão positiva dessas demandas numa nova identidade, no caso a identidade bolivariana. A pesquisa conclui que o movimento bolivariano consegue gerar elementos para permitir uma nova lógica de construção de sentido, configurando assim estruturalmente um campo de luta pela hegemonia. Porém, também são destacados vários problemas nessa tarefa, especialmente ligados à identificação de diversas unidades com o bolivarianismo.The dissertation research the Bolivarian Movement, headed by Venezuela, as a new hegemonic project, still under construction, at Latin America and the Caribbean. This hegemonic project is discursively constructed in opposition to the historic Pan American hegemony, led by the U.S. In this way, the Bolivarian Alliance for the Peoples of Our America (ALBA) is approached as an international organization that seeks to align the Latin American countries and societies behind the Bolivarian Movment. The ALBA generates new ways of sense-making, as well as new norms of international relations. The research analyzes the speeches at the ALBA's presidential summits, using the Ernesto Laclau's Discourse Theory, and identifying the three steps proposed by this author to the hegemonic construction: Firstly, the establishment of an antagonism against U.S. imperialism and the dicotomization of the hemispheric social space, through the definition of a boundary between the powerful country and the rest of continental community (Latina America and the Caribe). Secondly, the contingent articulation of a variety of social demands, which dissatisfaction are ascribed to the U.S. imperialism. Thirdly, the formation of a new popular field, through the positive expression on a new identity of this unified set of demands, in this case the Bolivarian identity. The dissertation concludes that the Bolivarian movement manages a new logic of sense-making, and therefore places the hegemonic struggle in a new structural field. However, it also identified some problems on this task, particularly in the difficulty to construct a Bolivarian identity shared by various social unities (countries, societies and social sectors)
Venezuela tra Alba e Mercosur. Un ruolo ambivalente
No full textGiacomo Pellini, "Venezuela tra Alba e Mercosur", in: Visioni LatinoAmericane, 11 (2014), pp.48-62L’autore esamina l’attivismo di Hugo Châvez nei processi di integrazione latinoamericana soffermandosi in particolare sull’Alba e il Mercosur. Mette a confronto, da una parte, la leadership esercitata dal Venezuela all’interno dell’Alba e dall’altra il ruolo di secondo piano del Venezuela all’interno del Mercosur. Evidenzia come per il Paese sia più vantaggiosa la permanenza, seppur con un ruolo meno incisivo, nel Mercosur che non la leadership nell’Alba. El autor examina el activismo de Hugo Châvez en los procesos de integración en América Latina, con especial énfasis en el Alba y en el Mercosur. Compara, por un lado, el liderazgo ejercido por Venezuela en el Alba y, por el otro, su rol secundario en el Mercosur. Muestra cómo para el Pais sea mâs ventajosa la retención, aunque con un rol de menor importancia, en el Mercosur, respecto al liderazgo en el Alba. The author examines the activism of Hugo Chàvez in the main integration processes in Latin America, Alba and Mercosur. He examines, on one hand, the leadership that Venezuela practices within the Alba and, on the other hand, the secondary role of Venezuela within Mercosur. He explains that for the country is more advantageous to remain inside the Mercosur, despite the secondary role, than to exercise the leadership within the Alba
Policy analysis and public policy in the private sector
No full textThis chapter provides a description of policy analysis in the private sector. The author introduces the topic with an informative description of the organizations that have been historically created to represent businessmen and businesswomen. Thereafter, Alba presents the main research centres that have been established by private actors with the aim of getting accurate information about political and economic trends. Alba then shows how business has developed some capacity to do policy analysis in the areas of its interest and explains the various mechanisms that businesses employ in their attempts to negotiate with state actors, and thus to influence policymaking processes.</p
Parfums de Philippe Claudel, un abécédaire des odeurs.
No full textAfter having investigated in many of his novels the human soul and his weaknesses, Philippe Claudel gives readers, in his latest book entitled Parfums, the possibility to follow him in a journey backwards, which recalls his olfactory memories. We will try to show how, starting with those reminiscences, the author brings us to discover his world. Philippe Claudel materialize those scents, whether pleasant or unpleasant into images, turning them into snapshots. It will be impossible in this paper to detail all the scents that inhabit the sensory world of Claudel, however, we will attempt to trace in this kaleidoscope of perfumes the aspects and issues with which the man and the writer must face
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